scholarly journals hemical Composition and Biological Activity of Ixiolirion tataricum (Pall.) Schult. & Schult.f. var. tataricum.

2021 ◽  
Vol 1 (1-2) ◽  
pp. 27-34
Author(s):  
Serkan Yigitkan ◽  
Mehmet Akdeniz ◽  
İsmail Yener ◽  
Mehmet Firat ◽  
Kerem Senturk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Life ◽  
Chemical Properties ◽  
Ethanol Extract ◽  
Quinic Acid ◽  
Total Phenolic ◽  
Aerial Parts ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

The role of plants in human life and the importance of plants in the food, drug and cosmetics industry have been increasing nowadays. This study investigates biological and chemical properties of root, branch, leaf, flower and aerial ethanol extracts of Ixiolirion tataricum (Pall.) Schult. & Schult. f. var. tataricum species, consumed as food in the population. The chemical composition of the species has been determined by the LC-MS/MS by specifying total phenolic and flavonoid contents. Antioxidant activity, cholinesterase, urease, tyrosinase, elastase and collagenase inhibitory activities have also been studied. According to the results of LC-MS/MS, quinic acid, protocatechuic acid, p-coumaric acid, and rutin have been detected. The branch ethanol extract contained higher amounts of quininic acid (12.101 µg analyte/g extract) than extracts of leaves, flowers, roots and aerial parts. On the other hand, the leaves ethanol extract exhibited high antioxidant activity in all tests (ABTS: IC50= 57.47±0.86 µg/mL, CUPRAC: A0.5= 66.76±1.86, DPPH: IC50: 119.2±1.52). The species has the potential to be used in the food industry due to its high antioxidant capacity.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

scholarly journals Antioxidant Activity and Total Phenolic Content of Stingless Bee Propolis from Indonesia

2019 ◽  
Vol 63 (1) ◽  
pp. 139-147
Author(s):  
Al Mukhlas Fikri ◽  
Ahmad Sulaeman ◽  
Sri Anna Marliyati ◽  
Mokhamad Fahrudin
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Stingless Bee ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Contents ◽  
South Sulawesi ◽  
Assisted Extraction ◽  
Dpph Method ◽  
Ethanol Extracts

AbstractThe aim of this study was to investigate antioxidant activity, total phenolic compounds and flavonoids of propolis from three provinces of Indonesia with two methods of extraction. Stingless bee propolis from Banten, South Kalimantan, and South Sulawesi was prepared through ultrasound-assisted extraction with water and ethanol solvents, separately. Antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid contents were analyzed by Folin-Ciocalteu colorimetric and aluminum chloride (AlCl3) methods, respectively. The result showed that the antioxidant activity expressed as IC50 of Indonesian propolis ranged from 452.52 to 1027.29 mg/L. Ethanol extracts showed higher antioxidant activity than water extracts. Total phenolic and flavonoids content detected in the samples ranged from 10.00 to 28.65 mg/g GAE and 0.76 to 3.39 mg/g QE, respectively. The ethanol extract of propolis from South Kalimantan had the highest total phenolic contents, while that from South Sulawesi possessed the highest total flavonoids.

scholarly journals Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo

2019 ◽  
Vol 16 (2) ◽  
pp. 64
Author(s):  
Didin Erma Indahyani ◽  
Depi Praharani ◽  
Izzata Barid ◽  
Ari Tri Wanodyo Handayani
Keyword(s):  
Antioxidant Activity ◽  
Brown Seaweed ◽  
Water Extract ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Red Seaweed ◽  
Water Extracts ◽  
High Antioxidant Activity ◽  
Polysaccharide Composition ◽  
Ethanol Extracts

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.

scholarly journals Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L.

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 229-244 ◽  
Author(s):  
Sinem Aydin
Keyword(s):  
Antioxidant Activity ◽  
Brassica Oleracea ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Ft Ir ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Brassica oleracea var. acephala and Ornithogalum umbellatum are two plant species which belong Brassicaceae and Liliaceae, respectively. Both of them are used as food and medicinally. O. umbellatum is used as anticancer, diuretic anti anti emetic. B. oleracea var. acephala is used for gastritis, gastric and duodenal ulcers and preventing some cancer forms such as stomach cancer, breast cancer and lung cancer. The aim of this research was to search antioxidant, antibacterial and antifungal activities and FT-IR analyses of ethanol extracts of stem and flower parts of B. oleracea var. acephala and O. umbellatum. Extracts were obtained by Soxhlet extraction. Ethanol extracts of B. oleracea var. acephala showed higher antibacterial activity than ethanol extracts of O. umbellatum. The most effective antifungal extract was ethanol extract of flower part of O. umbellatum against the fungi Candida. tropicalis and Candida. parapsilosis. Antioxidant activity of the extracts were also studied. The highest total phenolic and flavonoid contents were found in flower part of B. oleracea var. acephala as 50.7?0.007 ?g GAE/mL and stem part of O. umbellatum as 81.37?0.006 ?g CE/mL, respectively. BHT which used as standard antioxidant had higher total antioxidant activity than tested ethanol extracts. DPPH activity of the extracts increased in the following order: Flower part of B. oleracea var. acephala> Stem part of O. umbellatum> Flower part of O. umbellatum> Stem part of B. oleracea var. acephala. These results which obtained from this study demonstrate that B. oleracea var. acephala and O. umbellatum species might be utilized as an alternative to synthetic antioxidans and antimicrobials.

scholarly journals Phenolic Combination and Comparison of Antioxidant Activity in Three Different Alcoholic Extracts of Dracocephalum moldavica L.

2017 ◽  
Vol 5 (3) ◽  
pp. 199 ◽  
Author(s):  
Behnaz Aslanipour ◽  
Reza Heidari ◽  
Neda Farnad
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reducing Ability ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Dracocephalum moldavica L. has been used as both treatment of coronary heart disorders and food supplements. In the present study, determination of total phenolic and flavonoid content in methanol, ethanol and methanol/ethanol extracts of leaf was studied in Iranian Moldavian balm for the first time. Antioxidant activity of the studied plant was also measured. The methanol extract had the highest phenolic and flavonoid content, anthocyanin, DPPH and H2O2 radical scavenging activity. The ethanol extract showed the least amount of all. The methanol/ethanol extract showed the highest amount in two oxides including nitric and superoxide radical scavenging activities; it also showed the highest Ferric Reducing Ability Power. The obtained chromatograms of the plant using High Performance Liquid Chromatography showed that the highest and the lowest found phenolic compounds were caffeic acid and vanilic acid, respectively. The results show that this plant is a suitable natural antioxidant to reduce the oxidative stress in human being.

scholarly journals TOTAL PHENOL AND ANTIOXIDANT POTENTIAL OF FIVE SPECIES OF LAMIACEAE FAMILY

2019 ◽  
Vol 16 (32) ◽  
pp. 239-249
Author(s):  
Sthéfane Guimarães ARAÚJO ◽  
Paula Avelar AMADO ◽  
Maria Eduarda Amaral PINTO ◽  
Ana Hortência Fonsêca CASTRO ◽  
Luciana Alves Rodrigues dos Santos LIMA
Keyword(s):  
Antioxidant Activity ◽  
Volatile Compounds ◽  
Harmful Effect ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Melissa Officinalis ◽  
Ic50 Value ◽  
Ethanol Extracts ◽  
Low Activity

Lamiaceae family has a cosmopolitan distribution, including about 300 genus and 7500 species. Numerous diseases have been related to free radicals overproduction. Antioxidant capacity is related to compounds capable of protecting a biological system against the potentially harmful effect of processes or reactions involving reactive oxygen and nitrogen species. In the present study, the ethanol extracts, obtained by maceration of Melissa officinalis, Mentha sp., Ocimum basilicum, Plectranthus barbatus, and Rosmarinus officinalis were evaluated for antioxidant activity, the total phenolic content was determined, and volatile compounds were identified by Gas Chromatography coupled to Mass Spectrometry (GC-MS). Ethanol extracts of M officinalis, Mentha sp., P. barbatus, and R. officinalis were able to reduce the free radical DPPH with IC50 of 3.81, 11.89, 6.92 and 11.54 μg/ml respectively, presenting better activity than the BHT, used as a standard. However, the ethanol extract of O. basilicum showed low activity and high IC50 value. Total phenols of all extracts ranged from 85.18 to 212.74 μg/ml. The highest phenolic concentration was observed in the ethanol extract of M. officinalis. Analysis of volatile compounds showed the presence of phytol in all extracts, being predominant in P. barbatus and R. officinalis, and also nonadecane and heptadecane in M. officinalis. Fractions obtained from the five species of Lamiaceae also presented antioxidant effect. In the most active fractions were mainly phenolic compounds, such as flavonoids, tannins, and coumarins. These results encourage new studies with these species in order to isolate the compounds responsible for this antioxidant activity so that in the future they can be used as natural antioxidants.

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

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