scholarly journals Macroscopic and Microscopic Evaluation of Galanthus woronowii Losinsk. and Galanthus nivalis L. Homeopathic Crude Herbal Drugs

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Bokov D O. ◽  
Samylina I. A. ◽  
Nikolov S. D.
Keyword(s):  
Herbal Drugs ◽  
Leaf Epidermis ◽  
Outer Scale ◽  
Diagnostic Features ◽  
Linear Dimensions ◽  
Cell Structures ◽  
Cellular Inclusions ◽  
Microscopic Evaluation ◽  
Galanthus Nivalis ◽  
Micro Level

Galanthus woronowii Losinsk. and Galanthus nivalis L. are two snowdrop species that are used in traditional medicine and homeopathy for nervous system disorders treatment. During pharmacognostic research linear dimensions of G. woronowii and G. nivalis homeopathic crude herbal drugs (HomCHD) were determined. The following microscopic anatomical and diagnostic features of snowdrop species were investigated: the structure of the adaxial and abaxial leaf epidermis, peduncle epidermis, the corolla epidermis, external and internal epidermis of outer scale, external and internal epidermis of storage scale, cell size and cellular inclusions (calcium oxalate raphides, starch grains). There are differences in the linear dimensions both in the macro and in the micro level. Overall dimensions of G. woronowii organs are significantly greater than G. nivalis organs, this fact is also reflected in the linear dimensions of the cell structures. The complex of diagnostic features allows the identification of the snowdrop species.

scholarly journals PHARMACOGNOSTIC INVESTIGATION OF GALANTHUS WORONOWII LOSINSK. AND GALANTHUS NIVALIS L. HERBAL PHARMACEUTICAL SUBSTANCES (MICROSCOPIC AND MACROSCOPIC ANALYSIS)

2018 ◽  
Vol 11 (10) ◽  
pp. 212
Author(s):  
Dmitry Olegovich Bokov
Keyword(s):  
Digital Camera ◽  
Leaf Epidermis ◽  
Outer Scale ◽  
Diagnostic Features ◽  
Pharmaceutical Substances ◽  
Linear Dimensions ◽  
Cell Structures ◽  
Cellular Inclusions ◽  
Diagnostic Signs ◽  
Galanthus Nivalis

Objective: Today drug produced from snowdrop species (Galanthus woronowii Losinsk. and Galanthus nivalis L.) used in Russian traditional medicine for nervous and cardiovascular systems disorders treatment.Pharmacognostic study of fresh snowdrop plants including macroscopic and microscopic (morpho-anatomical diagnostic features) evaluation for identification of herbal pharmaceutical substances (HPS).Methods: Macro- and micro-scopic evaluation was carried out according to general pharmacopeial monographs of State Pharmacopoeia of Russian Federation XIII ed., Photographs were obtained by the microscope “Altami 139T” (10× eyepiece and lenses: 4×, 10×, 40×, 100×) with a digital camera eyepiece UCMOS05100KPA; images were processed using Altami Studio program.Results: In a pharmacognostic study of G. nivalis and G. woronowii HPS linear dimensions were determined. Several microscopic diagnostics and anatomical signs of snowdrops were investigated: Adaxial and abaxial leaf epidermis; epidermis of corolla, peduncle; internal and external outer scale epidermis, internal and external storage scale epidermis, and sizes of cells and cellular inclusions (starch grains and calcium oxalate raphides). G. woronowii and G. nivalis HPS possess differences both in the micro and macro levels in the linear dimensions. In general, dimensions of G. nivalis organs are much smaller than G. woronowii ones, this aspect is also expressed in the cell structures linear dimensions. The complex of macro- and micro- diagnostic signs allows to identify the snowdrop species.Conclusion: The results of the investigation can be used in routine quality control and for inclusion of pharmacopeial monographs for snowdrop HPSs.

scholarly journals A preliminary pharmacognostical study on leaves and flowers of Michelia champaca L. Magnoliaceae

2011 ◽  
Vol 3 (2) ◽  
pp. 228-231
Author(s):  
K. N. Geetha ◽  
K. Jeyaprakash ◽  
Y. P. Nagaraja
Keyword(s):  
Amino Acid ◽  
Microscopic Analysis ◽  
Phytochemical Analysis ◽  
Herbal Drugs ◽  
Taxonomic Level ◽  
Cell Structures ◽  
Michelia Champaca

The present investigation was conducted to establish pharmacognostical profile for the leaves and flowers of Michelia champaca L. (Magnoliaceae) in order to establish its complete profile to aid in its identification and avoid confusion in taxonomic level for different species of the same genus. The study included macroscopical, organoleptical, microscopical and preliminary phytochemical analysis of the leaves and flowers. The study of the organoleptical evaluation revealed the presence of colour, odour and texture. The microscopic analysis showed thedifferences in cell structures, arrangement and shape of leaves and flowers. The physical characters of various solvent extracts showed the presence of colour, odour and consistency of the powdered leaves and flowers. Finally, the preliminary phytochemical analysis confirmed for the presence of alkaloids, saponins, tannins, glycosides, carbohydrates, amino acid, flavonoids and sterols in both leaves and flowers. The present findings may be used to establish the authenticity of leaves and flowers of Michelia champaca L. for their proper identification and standardization in order to collect raw plants for the preparation of herbal drugs.

scholarly journals Morpho-anatomical aspects of the firstand-second year leaf epidermis of some Rhododendron L. winter-green species in the Bugsko-Polessky Region

2017 ◽  
Vol 6 (1) ◽  
pp. 14-19
Author(s):  
Yulia Vladimirovna Bondar ◽  
Sergey Vladimirovich Zerkal
Keyword(s):  
Cross Sections ◽  
Botanical Garden ◽  
Leaf Epidermis ◽  
National Academy Of Sciences ◽  
Diagnostic Features ◽  
Adaptation Capacity ◽  
Mass Reproduction ◽  
Leaf Anatomical Structure ◽  
Second Year ◽  
Academy Of Sciences

This paper discusses morphological and anatomical aspects of the first-and-second-year-leaf epidermis of the two winter-green species, genus Rhododendron L.: R. catawbiense Michx. and R. davidsonianum Rehd., grown in the Bugsko-Polessky Region from the seed reproduction of the Central Botanical Garden of the National Academy of Sciences of Belarus. The representatives of the studied genus are promising crops for planting gardens, settlements and interiors. Therefore, the study of leaf anatomy lets to find plants adaptive features to different environmental conditions and identify their adaptation capacity in the new conditions of growth. The paper identified diagnostic features, as well as similarities and differences of morphometric parameters. The research method was a comparative anatomical one. The author made a code of diagnostic features of the leaf anatomical structure, which described the views of cross sections. The character of the natural confinement species lays its mark on the formation of individual elements of the leaves structure, ensuring their successful adaptation to the new conditions of growth. The studies have shown that both species quite successfully acclimatized and are promising for mass reproduction and wider use in the Bugsko-Polessky Region, and this is supported by qualitative and quantitative indicators of the morphology and internal leaf structure.

scholarly journals Deep learning extended depth-of-field microscope for fast and slide-free histology

2020 ◽  
Vol 117 (52) ◽  
pp. 33051-33060
Author(s):  
Lingbo Jin ◽  
Yubo Tang ◽  
Yicheng Wu ◽  
Jackson B. Coole ◽  
Melody T. Tan ◽  
...  
Keyword(s):  
Deep Learning ◽  
Reconstruction Algorithm ◽  
Surgical Margins ◽  
Phase Mask ◽  
Depth Of Field ◽  
Diagnostic Features ◽  
Thin Sections ◽  
Microscopic Evaluation ◽  
Resected Tissue ◽  
Tissue Specimens

Microscopic evaluation of resected tissue plays a central role in the surgical management of cancer. Because optical microscopes have a limited depth-of-field (DOF), resected tissue is either frozen or preserved with chemical fixatives, sliced into thin sections placed on microscope slides, stained, and imaged to determine whether surgical margins are free of tumor cells—a costly and time- and labor-intensive procedure. Here, we introduce a deep-learning extended DOF (DeepDOF) microscope to quickly image large areas of freshly resected tissue to provide histologic-quality images of surgical margins without physical sectioning. The DeepDOF microscope consists of a conventional fluorescence microscope with the simple addition of an inexpensive (less than $10) phase mask inserted in the pupil plane to encode the light field and enhance the depth-invariance of the point-spread function. When used with a jointly optimized image-reconstruction algorithm, diffraction-limited optical performance to resolve subcellular features can be maintained while significantly extending the DOF (200 µm). Data from resected oral surgical specimens show that the DeepDOF microscope can consistently visualize nuclear morphology and other important diagnostic features across highly irregular resected tissue surfaces without serial refocusing. With the capability to quickly scan intact samples with subcellular detail, the DeepDOF microscope can improve tissue sampling during intraoperative tumor-margin assessment, while offering an affordable tool to provide histological information from resected tissue specimens in resource-limited settings.

scholarly journals Standardization of the Ayurvedic Formulation Haridra Khanda Using High-Performance Thin-Layer Chromatography-Densitometry

2008 ◽  
Vol 91 (5) ◽  
pp. 1162-1168 ◽  
Author(s):  
Kedar Kumar Rout ◽  
Sagarika Parida ◽  
Sagar Kumar Mishra
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Curcuma Longa ◽  
Water Soluble ◽  
Trace Metal Analysis ◽  
Cell Structures ◽  
Microscopic Evaluation ◽  
Multiple Wavelengths ◽  
Layer Chromatography ◽  
Instrumental Precision

Abstract The present study aimed to standardize the Ayurvedic preparation Haridra Khanda containing Curcuma longa as a major ingredient. Various physicochemical parameters such as alcohol-soluble extractive, water-soluble extractive, total ash, and acid-insoluble ash were determined according to the Ayurvedic Pharmacopoeia of India. Microscopic evaluation of the formulation revealed the presence of various diagnostic cell structures of C. longa. Trace metal analysis indicated the absence of toxic metals such as As, Cd, Hg, and Pb. High-performance thin-layer chromatographic (HPTLC) fingerprint patterns at multiple wavelengths (254, 366, and 430 nm) identified the number of components present at each wavelength. The bioactive markers curcumin (C1), demethoxycurcumin (C2), and bisdemethoxycurcumin (C3) were quantified by using a simple, rapid, and efficient HPTLC method using plates precoated with silica gel 60F254 stationary phase. The instrumental precision [coefficient of variation (CV)] was 0.51, 0.64, and 0.79% and the repeatability of the method (CV) was 0.89, 1.11, and 0.95%, respectively, for C1 to C3. Limits of detection and quantitation for compounds C1 to C3 were 20, 20, and 15 ng and 50, 40, and 50 ng, respectively. Response was a linear function in the ranges of 50350, 40240, and 50300 ng with correlation coefficient (r) 0.9998, 0.9995, and 0.9992, respectively, for C1 to C3. The mean recovery values of 99.63 (C1), 98.65 (C2), and 98.97% (C3) indicated the excellent accuracy of the method. It is shown that HPTLC can be applied successfully for the marker evaluation of the formulation containing C. longa.

Video-Enhanced Microscopy--Better Visibility of Plant Cell Structures

1982 ◽  
Vol 40 ◽  
pp. 182-185
Author(s):  
N.S. Allen ◽  
R.D. Allen
Keyword(s):  
Diffraction Pattern ◽  
Theoretical Basis ◽  
Numerical Aperture ◽  
Gain Control ◽  
Control Unit ◽  
Camera Control ◽  
Variable Gain ◽  
Contrast Method ◽  
Fine Detail ◽  
Cell Structures

Various methods of video-enhanced microscopy combine TV cameras with light microscopes creating images with improved resolution, contrast and visibility of fine detail, which can be recorded rapidly and relatively inexpensively. The AVEC (Allen Video-enhanced Contrast) method avoids polarizing rectifiers, since the microscope is operated at retardations of λ/9- λ/4, where no anomaly is seen in the Airy diffraction pattern. The iris diaphram is opened fully to match the numerical aperture of the condenser to that of the objective. Under these conditions, no image can be realized either by eye or photographically. Yet the image becomes visible using the Hamamatsu C-1000-01 binary camera, if the camera control unit is equipped with variable gain control and an offset knob (which sets a clamp voltage of a D.C. restoration circuit). The theoretical basis for these improvements has been described.

Ovarian Dysfunction in Fetally Irradiated Beagles

1977 ◽  
Vol 35 ◽  
pp. 658-659
Author(s):  
T. M. Seed ◽  
M. H. Sanderson ◽  
D. L. Gutzeit ◽  
T. E. Fritz ◽  
D. V. Tolle ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Low Dose ◽  
Long Term Effects ◽  
Ovarian Dysfunction ◽  
Dose Rates ◽  
Highly Sensitive ◽  
Microscopic Evaluation ◽  
Ovarian Pathology ◽  
Normal Offspring

The developing mammalian fetus is thought to be highly sensitive to ionizing radiation. However, dose, dose-rate relationships are not well established, especially the long term effects of protracted, low-dose exposure. A previous report (1) has indicated that bred beagle bitches exposed to daily doses of 5 to 35 R 60Co gamma rays throughout gestation can produce viable, seemingly normal offspring. Puppies irradiated in utero are distinguishable from controls only by their smaller size, dental abnormalities, and, in adulthood, by their inability to bear young.We report here our preliminary microscopic evaluation of ovarian pathology in young pups continuously irradiated throughout gestation at daily (22 h/day) dose rates of either 0.4, 1.0, 2.5, or 5.0 R/day of gamma rays from an attenuated 60Co source. Pups from non-irradiated bitches served as controls. Experimental animals were evaluated clinically and hematologically (control + 5.0 R/day pups) at regular intervals.

Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

1991 ◽  
Vol 49 ◽  
pp. 34-35 ◽  
Author(s):  
P. Frayssinet ◽  
J. Hanker ◽  
D. Hardy ◽  
B. Giammara
Keyword(s):  
Hip Prosthesis ◽  
Ethanol Concentration ◽  
Bone Matrix ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Hip Prostheses ◽  
Cellular Inclusions ◽  
Microscopic Studies ◽  
Diamond Saw ◽  
Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

Selection and Preparation of Specific Tissue Regions For Tem Using Large Epoxy-Embedded Blocks

1973 ◽  
Vol 31 ◽  
pp. 324-325 ◽  
Author(s):  
P. M. Lowrie ◽  
W. S. Tyler
Keyword(s):  
Electron Microscopy ◽  
Anatomical Structures ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Embedded Blocks ◽  
Specific Tissue ◽  
Definition Of ◽  
Areas Of Interest ◽  
Selection Of

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).

TEM comparison of osmium vs osmium with potassium ferricyanide secondary fixatives and the impact of secondary fixative temperature on tissue preservation/contrast quality

1996 ◽  
Vol 54 ◽  
pp. 910-911
Author(s):  
J. W. Horn ◽  
B. J. Dovey-Hartman ◽  
V. P. Meador
Keyword(s):  
Osmium Tetroxide ◽  
Potassium Ferricyanide ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Cacodylate Buffer ◽  
Transmission Electron Microscopic ◽  
Glutaraldehyde Solution ◽  
Temperature Impact ◽  
The Impact

Osmium tetroxide (OsO4) is a universally used secondary fixative for routine transmission electron microscopic evaluation of biological specimens. Use of OsO4 results in good ultrastructural preservation and electron density but several factors, such as concentration, length of exposure, and temperature, impact overall results. Potassium ferricyanide, an additive used primarily in combination with OsO4, has mainly been used to enhance the contrast of lipids, glycogen, cell membranes, and membranous organelles. The purpose of this project was to compare the secondary fixative solutions, OsO4 vs. OsO4 with potassium ferricyanide, and secondary fixative temperature for determining which combination gives optimal ultrastructural fixation and enhanced organelle staining/contrast.Fresh rat liver samples were diced to ∼1 mm3 blocks, placed into porous processing capsules/baskets, preserved in buffered 2% formaldehyde/2.5% glutaraldehyde solution, and rinsed with 0.12 M cacodylate buffer (pH 7.2). Tissue processing capsules were separated (3 capsules/secondary fixative.solution) and secondarily fixed (table) for 90 minutes. Tissues were buffer rinsed, dehydrated with ascending concentrations of ethanol solutions, infiltrated, and embedded in epoxy resin.

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