scholarly journals A preliminary pharmacognostical study on leaves and flowers of Michelia champaca L. Magnoliaceae

2011 ◽  
Vol 3 (2) ◽  
pp. 228-231
Author(s):  
K. N. Geetha ◽  
K. Jeyaprakash ◽  
Y. P. Nagaraja
Keyword(s):  
Amino Acid ◽  
Microscopic Analysis ◽  
Phytochemical Analysis ◽  
Herbal Drugs ◽  
Taxonomic Level ◽  
Cell Structures ◽  
Michelia Champaca

The present investigation was conducted to establish pharmacognostical profile for the leaves and flowers of Michelia champaca L. (Magnoliaceae) in order to establish its complete profile to aid in its identification and avoid confusion in taxonomic level for different species of the same genus. The study included macroscopical, organoleptical, microscopical and preliminary phytochemical analysis of the leaves and flowers. The study of the organoleptical evaluation revealed the presence of colour, odour and texture. The microscopic analysis showed thedifferences in cell structures, arrangement and shape of leaves and flowers. The physical characters of various solvent extracts showed the presence of colour, odour and consistency of the powdered leaves and flowers. Finally, the preliminary phytochemical analysis confirmed for the presence of alkaloids, saponins, tannins, glycosides, carbohydrates, amino acid, flavonoids and sterols in both leaves and flowers. The present findings may be used to establish the authenticity of leaves and flowers of Michelia champaca L. for their proper identification and standardization in order to collect raw plants for the preparation of herbal drugs.

Comparative Pharmacognostic, Physicochemical and Phytochemical Studies of Different Samples of Plumbago zeylanica L. Roots

2017 ◽  
Vol 9 (06) ◽  
Author(s):  
Neha Jain ◽  
Mohan Lal Kori
Keyword(s):  
Phytochemical Analysis ◽  
Local Market ◽  
Herbal Drugs ◽  
Disease Treatment ◽  
Organoleptic Evaluation ◽  
Plumbago Zeylanica ◽  
Qualitative And Quantitative ◽  
Crude Drugs ◽  
Phytochemical Studies

The objective of the present study is to evaluate the quality of the marketed and self collected samples of Plumbago zeylanica L. roots on the standardization parameters. This study is planned mainly to confirm changes with quality of drug. Now a day’s more demand of herbal drugs for disease treatment, lack of knowledge of proper methodology and availability are promoting the practices of adulteration and substitution. Thus, the standardization of the plant crude drugs is necessary to maintain their therapeutic efficacy. Comparative studies were carried out to evaluate the standards of P. zeylanica L. with emphasis on organoleptic evaluation, physicochemical and phytochemical analysis. Samples were procured from local market and self collected to determine the qualitative and quantitative variations. The result indicates that self collected sample showed significant results with comparison to marketed sample.

THE EFFECT OF PROCESSING METHODS ON THE NUTRITIONAL QUALITY OF AFRICAN BREADFRUITS (TRECULIA AFRICANA) SEEDS

2020 ◽  
Vol 16 (2) ◽  
pp. 60
Author(s):  
Nwozo Sarah Onyenibe ◽  
Julius Oluwaseun Oluwafunmilola ◽  
Stanley Udogadi Nwawuba
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Quality ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Mineral Contents ◽  
Processing Methods ◽  
African Breadfruit

The extracted seeds of African breadfruit are identified to be extremely healthy whenever it is correctly processed. Therefore, the aim of the present study was to evaluate the effects of processing methods on the nutritional quality of African breadfruit seed. A qualitative phytochemical analysis including: Alkaloid, Flavonoid, Saponin, Tannin, Anthraquinone, Terpenoids, Steroid, and Cardiac Glycosides for the different fraction of African breadfruit seed was performed using a standard method. The result revealed the presence and greater amount of phytochemical for the raw fraction; seven in eight, six in eight for steamed fraction, and four in eight for boiled and roasted respectively. Anti-nutrient, Proximate, and Mineral Content were also conducted using standard methods. The amino acid composition was determined using High-Performance Liquid Chromatography (HPLC). The results of the present study revealed that anti-nutrients including Phytate, Tannins, and Oxalate were significantly p<0.05 reduced in the boiled fraction 5.47±0.15, 3.42±0.02 and 6.89±0.05, and highest in the raw fraction 7.77±0.01, 5.09±0.03 and 9.34±0.14. The proximate composition including; percentage crude fat, Ash, Carbohydrate, Fatty acid, and Energy value were significantly lower p<0.05 in the boiled fraction relative to the other fractions. Mineral contents; calcium, magnesium, sodium, potassium, and phosphorus were also significantly p<0.05 elevated in the boiled fraction relative to the raw, steamed, and roasted fraction. The amino acid composition was highest in the roasted and boiled fraction 57.350 and 56.978, and lowest in the steamed and raw fraction 35.754 and 28.748 respectively. Therefore, boiling (cooking) is encouraged for the preparation of African breadfruit seed.

scholarly journals Pharmacognostic Evaluation of Curcuma longa L. Rhizome and Standardization of its Formulation by HPLC Using Curcumin as Marker

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Abraham A ◽  
Samuel S ◽  
Mathew L
Keyword(s):  
Cross Section ◽  
Curcuma Longa ◽  
Microscopic Analysis ◽  
Fluorescence Analysis ◽  
Phytochemical Analysis ◽  
Diagnostic Features ◽  
Starch Grains ◽  
Qualitative And Quantitative ◽  
Polyherbal Formulation ◽  
Laboratory Procedures

Objective: To evaluate Curcuma longa rhizome by pharmacognostic and phytochemical analysis and standardize one of its formulations by HPLC. The present work includes macroscopic study, microscopic analysis of cross section and powder of rhizome, fluorescence analysis, qualitative and quantitative phytochemical assay and chromatographic fingerprinting of its polyherbal formulation, Pathyashadangam kwath using curcumin as marker carried out as per standard laboratory procedures. The study helps to provide both diagnostic features for identification and preventing adulteration of Curcuma longa L and HPLC chromatogram for standardisation of its formulation. Results: Microscopic analysis revealed the presence of a broad parenchymatous cortex with abundant starch grains in the cross section, spiral vessels, starch grains having hilum towards the narrower end, fibres and cells with oleo resin in powder microscopy. HPLC analysis revealed the presence of three peaks corresponding to curcuminoids in the formulation. Conclusion: Pharmacognostic and phytochemical evaluation can be used for confirming the identity of Curcuma longa rhizome and HPLC using curcumin as marker can be used for standardisation of its formulations.

scholarly journals Protein Digestibility of Cereal Products

Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 199 ◽  
Author(s):  
Iris Joye
Keyword(s):  
Amino Acid ◽  
Protein Quality ◽  
Intact Cell ◽  
Protein Digestibility ◽  
Amino Acid Profile ◽  
Antinutritional Factors ◽  
Internal Factors ◽  
Cereal Products ◽  
Low Protein ◽  
Cell Structures

Protein digestibility is currently a hot research topic and is of big interest to the food industry. Different scoring methods have been developed to describe protein quality. Cereal protein scores are typically low due to a suboptimal amino acid profile and low protein digestibility. Protein digestibility is a result of both external and internal factors. Examples of external factors are physical inaccessibility due to entrapment in e.g., intact cell structures and the presence of antinutritional factors. The main internal factors are the amino acid sequence of the proteins and protein folding and crosslinking. Processing of food is generally designed to increase the overall digestibility through affecting these external and internal factors. However, with proteins, processing may eventually also lead to a decrease in digestibility. In this review, protein digestion and digestibility are discussed with emphasis on the proteins of (pseudo)cereals.

scholarly journals Rho1p-Bni1p-Spa2p Interactions: Implication in Localization of Bni1p at the Bud Site and Regulation of the Actin Cytoskeleton inSaccharomyces cerevisiae

1998 ◽  
Vol 9 (5) ◽  
pp. 1221-1233 ◽  
Author(s):  
Takeshi Fujiwara ◽  
Kazuma Tanaka ◽  
Akihisa Mino ◽  
Mitsuhiro Kikyo ◽  
Kazuo Takahashi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Actin Cytoskeleton ◽  
Binding Protein ◽  
Microscopic Analysis ◽  
Mitogen Activated Protein Kinase ◽  
Gene Encoding ◽  
Amino Acid Region ◽  
Genes Encoding ◽  
Mitogen Activated Protein ◽  
Acid Region

Rho1p is a yeast homolog of mammalian RhoA small GTP-binding protein. Rho1p is localized at the growth sites and required for bud formation. We have recently shown that Bni1p is a potential target of Rho1p and that Bni1p regulates reorganization of the actin cytoskeleton through interactions with profilin, an actin monomer-binding protein. Using the yeast two-hybrid screening system, we cloned a gene encoding a protein that interacted with Bni1p. This protein, Spa2p, was known to be localized at the bud tip and to be implicated in the establishment of cell polarity. The C-terminal 254 amino acid region of Spa2p, Spa2p(1213–1466), directly bound to a 162-amino acid region of Bni1p, Bni1p(826–987). Genetic analyses revealed that both thebni1 and spa2 mutations showed synthetic lethal interactions with mutations in the genes encoding components of the Pkc1p-mitogen-activated protein kinase pathway, in which Pkc1p is another target of Rho1p. Immunofluorescence microscopic analysis showed that Bni1p was localized at the bud tip in wild-type cells. However, in the spa2 mutant, Bni1p was not localized at the bud tip and instead localized diffusely in the cytoplasm. A mutant Bni1p, which lacked the Rho1p-binding region, also failed to be localized at the bud tip. These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p.

scholarly journals A STUDY ON THE STANDARDIZATION PARAMETERS OF CASSIA ANGUSTIFOLIA

2017 ◽  
Vol 10 (7) ◽  
pp. 329 ◽  
Author(s):  
Pragati Khare ◽  
Kamal Kishore ◽  
Dinesh Kumar Sharma
Keyword(s):  
Amino Acid ◽  
Tamil Nadu ◽  
Uv Light ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Pharmacological Properties ◽  
Cassia Angustifolia ◽  
Fluorescence Characteristics

Objective: Now-a-days, the herbal medicines are much efficient for the treatment of various disorders as they have minimal side effects in comparison to the allopathic medicines. Cassia angustifolia, commonly called Senna belongs to the family Leguminosae and is a well-known laxative throughout the world. Senna is mostly found in Tirunelveli, Madurai, and Ramnathpuram districts of Tamil Nadu. Carbohydrates, tannins, alkaloids, flavonoids, and amino acid are the important chemical constituents of C. angustifolia. The objectives of the present study are to investigate various pharmacognostic, phytochemical analysis, and pharmacological properties of C. angustifolia.Methods: The powdered drug was used for estimating the loss on drying, ash values, fluorescence studies, chemical tests, and extractive values. Macroscopic and microscopic studies were also performed.Results: The transverse section (T.S). of leaf showed isobilateral structure along with paracytic stomata, nonlignified unicellular trichomes with warty walls, and fibrovascular bundle. The fluorescence characteristics of leaf powder were studied both in visible light and ultraviolet (UV) light (254 nm and 365 nm) after treatment with various reagents. Senna is composed of carbohydrates, tannins, alkaloids, flavonoids, and amino acid. It was reported that the total ash value was 11.23±0.25 w/w. The acid insoluble ash value was 1.4±0.1% w/w. Water soluble, ethanol, methanol, petroleum ether, and chloroform extractive values were 16.6±0.26% w/w, 3.7±1.75% w/w, 0.83±0.05% w/w, 1.6±0.1% w/w, and 3.2±0.25% w/w, respectively.Conclusion: The main pharmacological activities of Bauhinia variegata are anthelmintic, antiulcer, antitumor, antimicrobial, antidiabetic, anti-inflammatory, antigoitrogenic, and hepatoprotective. The present investigation provides the information on its pharmacognostic, phytochemical analysis, and pharmacological properties.

Phytochemical characterization and cytotoxicity analysis of ayurvedic formulation of drug

2020 ◽  
Vol 11 (3) ◽  
pp. 3164-3170
Author(s):  
Cynthia C ◽  
Horne Iona Averal
Keyword(s):  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Herbal Drugs ◽  
Endocrine Organ ◽  
Liver Disorders ◽  
Plant Drugs ◽  
Vero Cell Lines

The liver is a major endocrine organ which controls the significant metabolic activity in the human body. Treatment of several liver disorders with herbal based plant drugs is popularising nowadays due to their hepatoprotective and nontoxic effect. Livoral, a polyherbal Ayurvedic formulation is one such drug commonly recommended for liver disorders in India. The current work was aimed at studying the phytochemical characterisation and cytotoxic activity of livoral. Phytochemical analysis of extracts of livoral in methanol, hexane, acetone and petroleum ether was studied by TLC method. The cytotoxic activity of livoral was analysed by MTT assay in Vero cell lines at a concentration ranging from 10µg/ml to 500µg/ml. The phytochemical analysis revealed the presence of Phyto compound such as tannins, phenols, steroids, alkaloids, terpenes and carbohydrates in all the four types of livoral extracts. Phytochemicals such as phenols, terpenes and alkaloids were already known for their hepatoprotective activity. MTT assay revealed the nontoxic effect of livoral up to 300 µg/ml concentrations. Thus, based on the results of the phytochemical and cytotoxic analysis, the livoral was proven to be one of the best herbal drugs for liver disorders with hepatoprotective constituents and non-cytotoxic effect.

scholarly journals Presence of an isoform of H+-pyrophosphatase located in the alveolar sacs of a scuticociliate parasite of turbot: physiological consequences

Parasitology ◽  
2016 ◽  
Vol 143 (5) ◽  
pp. 576-587 ◽  
Author(s):  
NATALIA MALLO ◽  
JESÚS LAMAS ◽  
ANA-PAULA DEFELIPE ◽  
MARIA-EUGENIA DECASTRO ◽  
ROSA-ANA SUEIRO ◽  
...  
Keyword(s):  
Amino Acid ◽  
Physiological Role ◽  
Amino Acid Sequences ◽  
Cell Structures ◽  
Gene And Protein Expression ◽  
Conserved Gene ◽  
Eukaryotic Organisms ◽  
Cellular Compartments ◽  
First Time

SUMMARYH+-pyrophosphatases (H+-PPases) are integral membrane proteins that couple pyrophosphate energy to an electrochemical gradient across biological membranes and promote the acidification of cellular compartments. Eukaryotic organisms, essentially plants and protozoan parasites, contain various types of H+-PPases associated with vacuoles, plasma membrane and acidic Ca+2storage organelles called acidocalcisomes. We used Lysotracker Red DND-99 staining to identify two acidic cellular compartments in trophozoites of the marine scuticociliate parasitePhilasterides dicentrarchi: the phagocytic vacuoles and the alveolar sacs. The membranes of these compartments also contain H+-PPase, which may promote acidification of these cell structures. We also demonstrated for the first time that theP. dicentrarchiH+-PPase has two isoforms: H+-PPase 1 and 2. Isoform 2, which is probably generated by splicing, is located in the membranes of the alveolar sacs and has an amino acid motif recognized by the H+-PPase-specific antibody PABHK. The amino acid sequences of different isolates of this ciliate are highly conserved. Gene and protein expression in this isoform are significantly regulated by variations in salinity, indicating a possible physiological role of this enzyme and the alveolar sacs in osmoregulation and salt tolerance inP. dicentrarchi.

scholarly journals Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease

1993 ◽  
Vol 90 (23) ◽  
pp. 11282-11286 ◽  
Author(s):  
K Uéda ◽  
H Fukushima ◽  
E Masliah ◽  
Y Xia ◽  
A Iwai ◽  
...  
Keyword(s):  
Amino Acid ◽  
Alzheimer Disease ◽  
Amyloid Fibrils ◽  
Protein A ◽  
Microscopic Analysis ◽  
Amino Acid Sequences ◽  
Full Length ◽  
Peptide Sequence ◽  
Electron Microscopic ◽  
Hydrophobic Domain

A neuropathological hallmark of Alzheimer disease (AD) is a widespread amyloid deposition. We analyzed the entire amino acid sequences in an amyloid preparation and found, in addition to the major beta/A4-protein (A beta) fragment, two unknown peptides. We raised antibodies against synthetic peptides using subsequences of these peptides. These antibodies immunostained amyloid in neuritic and diffuse plaques as well as vascular amyloid. Electron microscopic analysis demonstrated that the immunostaining was localized on amyloid fibrils. We have isolated an apparently full-length cDNA encoding a 140-amino-acid protein within which two previously unreported amyloid sequences are encoded in tandem in the most hydrophobic domain. We tentatively named this 35-amino acid peptide NAC (non-A beta component of AD amyloid) and its precursor NACP. NAC is the second component, after A beta, identified chemically in the purified AD amyloid preparation. Secondary structure predictions indicate that the NAC peptide sequence has a strong tendency to form beta-structures consistent with its association with amyloid. NACP is detected as a M(r) 19,000 protein in the cytosolic fraction of brain homogenates and comigrates on immunoblots with NACP synthesized in Escherichia coli from NACP cDNA. NACP mRNA is expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver, suggesting its ubiquitous and brain-specific functions. The availability of the cDNA encoding full-length NACP should help to elucidate the mechanisms of amyloidosis in AD.

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