scholarly journals Nanomaterial Carbon-Dots Berbahan Dasar Daun Sirih (Piper Betle L.) Sebagai Antibakteri Terhadap Bakteri S. Mutans dan E. Coli

POSITRON ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 26
Author(s):  
Emi Kurnia Sari ◽  
Danvi Sekartaji ◽  
Athi' Nur Auliati Rahmah ◽  
Wipsar Sunu Brams Dwandaru
Keyword(s):  
Surface State ◽  
Carbon Dots ◽  
Piper Betle ◽  
E Coli

Penelitian ini bertujuan untuk mensintesis, mengkarakterisasi, dan mengetahui peran nanomaterial carbon-dots (Cdots) sebagai antibakteri terhadap bakteri S. mutans dan E. coli. Cdots dibuat dengan bahan dasar daun sirih (Piper Betle L.) menggunakan metode pemanasan oven. Terdapat tiga buah sampel Cdots yang dihasilkan yaitu 0,5 g serbuk daun sirih+aquades (Cdots A), 0,5 g serbuk daun sirih+ekstrak daun sirih (Cdots B), dan 1 g serbuk daun sirih+ekstrak daun sirih (Cdots C). Ketiga sampel memiliki karakteristik yang hampir sama, yaitu adanya puncak absorbansi pada rentang panjang gelombang 257nm – 320 nm. Daya serap yang tinggi pada rentang ultraviolet (UV) merupakan salah satu sifat yang dimiliki Cdots. Selain itu, ketiga sampel Cdots memiliki pendaran biru-kehijauan (cyan) ketika dikenai laser UV.Hal ini merupakan sifat luminesensCdots yang dapat berpendar pada panjang gelombang cahaya tampak. Cdots tersusun dari core dan surface state yang masing-masing ditunjukkan dengan adanya gugus fungsi C=C dan gugus O-H serta C-O yang terdeteksi oleh uji FTIR dari ketiga sampel Cdots. Pengujian antibakteri menggunakan metode Kirby-Bauer menunjukkan sampel Cdots C memiliki aktivitas antibakteri tertinggi karena memiliki konsentrasi Cdots yang lebih banyak dan bekerja sama dengan ekstrak sirih yang memiliki sifat antibakteri sehingga berpotensi sebagai agen antibakteri dibandingkanekstrak daun sirih murni.

scholarly journals Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis

2020 ◽  
Vol 4 (2) ◽  
pp. 230-236
Author(s):  
Dicky Rizaldi Rambe ◽  
Allaily Allaily ◽  
Cut Intan Novita
Keyword(s):  
Leaf Extract ◽  
Agricultural Product ◽  
Piper Betle ◽  
Product Analysis ◽  
E Coli ◽  
Betel Leaf ◽  
Randomized Design ◽  
Inhibitory Analysis ◽  
Analysis Laboratory ◽  
Range Test

Abstrak. Penelitian tentang Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis telah dilaksanakan di Laboratorium Ilmu dan Teknologi Pengolahan Susu dan Laboratorium Analisis Pangan dan Hasil Pertanian yang dilaksanakan pada 7 September sampai dengan 10 November 2018. Penelitian ini terdiri dari 4 perlakuan dan 5 ulangan. Data yang diperoleh selanjutnya akan diolah menggunakan Rancangan Acak Lengkap, jika didapatkan hasil yang berbeda maka akan dilanjutkan dengan uji Duncan. Perlakuan R1 yaitu penambahan ekstrak daun sirih hijau 15%, R2 yaitu penambahan ekstrak daun sirih hijau 30%, R3 yaitu penambahan ekstrak daun sirih hijau 45% dan R4 yaitu penambahan ekstrak daun sirih hijau 60%. Hasil penelitian menunjukkan bahwa ekstrak daun sirih hijau dengan konsentrasi 15%, 30%, 45% dan 60% mampu menghambat E. coli dan S. aureus. Berdasarkanhasil penelitian ini dapat disimpulkan bahwa ekstrak daun sirih hijau mampu menghambat pertumbuhan E. coli dan S. aureus.Inhibiting Power Analysis of Green Betel Leaf Extract (Piper betle L.) against Microorganism Indicator MastitisAbstract. Research on Inhibitory Analysis of Green Betel Leaf Extract (Piper betle L.) against Mastitis causing Microorganism has been conducted in Milk Processing Science and Technology Laboratory and  Food and Agricultural Product Analysis Laboratory held on September 7 to November 10, 2018. This research consisted of 4 treatments and 5 replications. The data obtained will then be processed using a Completely Randomized Design, if different results are obtained it will be followed by the Duncan Multiple Range Test. The treatment of R1 is the addition of 15% green betel leaf extract, R2, which is the addition of 30% green betel leaf extract, R3, namely the addition of 45% green betel leaf extract and R4 which is the addition of 60% green betel leaf extract. The results showed that green betel leaf extract with a concentration of 15%, 30%, 45% and 60% was able to inhibit E. coli and S. aureus. Based on the results of this study it can be concluded that green betel leaf extract can inhibit the growth of E. coli and S. aureus.

Facile synthesis of N,B-co-doped carbon dots with the gram-scale yield for detection of iron (III) and E. coli

2020 ◽  
Vol 31 (39) ◽  
pp. 395702
Author(s):  
Lixia Zhang ◽  
Zishu Zhang ◽  
Zhiwen Gao ◽  
Yan Xie ◽  
Shuang Shu ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Facile Synthesis ◽  
E Coli ◽  
Doped Carbon Dots ◽  
Co Doped

scholarly journals Antimicrobial Activity and Characterization of Pomegranate Peel-Based Carbon Dots

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Waseem Akhtar Qureshi ◽  
B. Vivekanandan ◽  
J. Altrin Jayaprasath ◽  
Daoud Ali ◽  
Saud Alarifi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Carbon Dots ◽  
Diffusion Method ◽  
Moderate Activity ◽  
E Coli ◽  
Analytical Instruments ◽  
Antimicrobial Efficiency ◽  
Microbial Efficiency ◽  
Ft Ir

This investigation reports the use of agrowaste pomegranate peels as an economical source for the production of fluorescent carbon dots (C-dots) and their potential application as an antimicrobial agent. The carbon dots were prepared through low-temperature carbonization at 200°C for 120 min. The obtained C-dots were found to be small in size and exhibited blue luminescence at 350 nm. Further, the synthesized C-dots were characterized with the help of analytical instruments such as DLS, UV-visible, FT-IR, TEM, and fluorescence spectrophotometer. Antimicrobial activity of the C-dot PP was estimated by the agar diffusion method and MIC. S. aureus and K. pneumoniae are showing susceptibility towards C-dot PP when compared to the standard and showing a moderate activity against P. aeruginosa and resistance towards E. coli. The obtained C dot PPs were found to be around 5-9 nm in size confirmed from DLS analysis and supported by TEM. The synthesized C-dots were investigated to understand their microbial efficiency against pathogens and found to have antimicrobial efficiency. These results suggest that pomegranate peels are a potential source of carbon dots with antimicrobial efficiency.

scholarly journals Antibacterial activity of Piper betle ethanolic extract against ESBL producing Escherichia coli in sepsis induced BALB/c mice

2021 ◽  
Vol 015 (02) ◽  
pp. 01-07
Author(s):  
Shod Abdurrachman Dzulkarnain ◽  
Irene Ratridewi ◽  
Vanianissa Hanareta Hantoko ◽  
Yessy Chairani Puspita Sari ◽  
Dewi Santosaningsih
Keyword(s):  
Escherichia Coli ◽  
Survival Rate ◽  
Alternative Therapy ◽  
Ethanolic Extract ◽  
Antimicrobial Effect ◽  
Dilution Method ◽  
Piper Betle ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Incidence And Mortality

Sepsis is an organ dysfunction caused by dysregulation of immune response towards systemic infection with high incidence and mortality rate. Innovative breakthrough is required to manage sepsis especially extended spectrum beta lactamase (ESBL) producing Escherichia coli (E. coli) induced sepsis. Utilization of Piper betle extract to manage sepsis could be considered as an alternative therapy and should be proven scientifically so meropenem utilization could be reduced. This study aimed to evaluate the antimicrobial effect of Piper betle ethanolic extract in BALB/c mice infected with ESBL producing E. coli. We identified terpene, apiol, polyphenol, eugenol, myristicin, flavonoid, safrole, lynalil acetate, and allyl tetramethoxy benzene in the Piper betle ethanolic extract using thin layer chromatography. The minimum inhibitory concentration of the Piper betle ethanolic extract against ESBL producing E. coli was 3 mg/mL assessed using broth dilution method. We evaluated the effect of Piper betle ethanolic extract to the survival rate of BALB/c mice infected with ESBL producing E. coli. The single dose administration of Piper betle ethanolic extract prolonged the survival rate of the infected BALB/c mice for 6 hours.

scholarly journals Biosynthesis of Zinc Oxide Nanoparticles Using Aqueous Piper betle Leaf Extract and Its Application in Surgical Sutures

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Quynh Mai Thi Tran ◽  
Hong Anh Thi Nguyen ◽  
Van-Dat Doan ◽  
Quang-Hieu Tran ◽  
Van Cuong Nguyen
Keyword(s):  
Developing Countries ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Piper Betle ◽  
Oxide Nanoparticles ◽  
Site Infection ◽  
Zno Nps ◽  
E Coli ◽  
Size And Morphology

Surgical site infection (SSI), mainly caused by Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), is considered the most frequent complication in a surgical patient. Globally, surgical site infection accounts for 2.5%-41.9% and even higher rates in developing countries. SSI affects not only the patient’s health but also the development of society. Like previous reports, a surgical suture increases the hazard of SSI due to its structure. The antibacterial suture is the most effective solution to decrease the SSI. Due to some unique properties, nano-zinc oxide (ZnO NPs) is one of the promising antibacterial agents for coating on the suture. In this study, we aim to synthesize the ZnO NPs using Piper betle leaf extract and used it to coat the suture. The effect of synthesis parameters on the size and morphology of ZnO NPs was studied as well. The UV-Vis spectrum indicated the formation of ZnO NPs with λ max at around 370 nm. The volume of leaf extract plays a role in controlling the size and morphology of zinc oxide nanoparticles. The average particle size of as-synthesized ZnO NPs was around 112 nm with a hexagonal and spherical shape. Other than that, the results proved that ZnO NPs performed a high antibacterial activity against S. aureus and E. coli with its antibacterial effectiveness up to 5 days. The ZnO NP-coated sutures also exhibited a high performance on bacterial inactivation. With key findings, this study made a tremendous contribution to lowering the burden on medical services in terms of medical treatment cost in developing countries.

scholarly journals Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

2021 ◽  
Author(s):  
Elizaveta Sviridova ◽  
Alexandre Barras ◽  
Evgenii Plotnikov ◽  
Antonio Di Martino ◽  
Dominique Deresmes ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Photoelectron Spectroscopy ◽  
Carbon Dots ◽  
Covalent Binding ◽  
Mouse Fibroblast ◽  
Fine Tuning ◽  
Gram Positive ◽  
Biofilm Inhibition ◽  
Gram Negative ◽  
E Coli

In this contribution, we report a facile functionalization method of carbon dots (CDs) by tetraalkylammonium moieties using diazonium chemistry to improve their antibacterial activity against Gram-positive and Gram-negative bacteria. CDs were modified by novel diazonium salts bearing tetraalkylammonium moieties (TAA) with different alkyl chains (C2, C4, C9, C12) for the optimization of antibacterial activity. The functionalized CDs were characterized by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FTIR) spectroscopy, ultraviolet–visible (UV-vis) absorption spectrophotometry and fluorescence spectroscopy to confirm the covalent binding of TAA onto the CDs’ surface. Variation of the alkyl chain allows to reach the strongest antibacterial effect for CDs-C9 towards Gram-positive S. aureus (MIC 3.09±1.10 µg mL-1) and Gram-negative E. coli (MIC 7.93±0.17 µg mL-1) bacteria. In addition, the antibiofilm properties of the CDs-C9 were assessed; full biofilm inhibition was achieved after 6 h (64 µg mL-1) and 2 h (128 µg mL-1) treatment for S. aureus biofilm and >60% suppression of biofilm mass after 6 h (128 µg mL-1) for E. coli biofilm. CDs-C9 demonstrated good biocompatibility on mouse fibroblast (NIH/3T3), HeLa and U-87 MG cell lines for concentrations up to 256 µg mL-1. Collectively, our work highlights the correlation between the surface chemistry of CDs and their antimicrobial performance.

Full-Color Light-Emitting Carbon Dots with a Surface-State-Controlled Luminescence Mechanism

ACS Nano ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. 484-491 ◽  
Author(s):  
Hui Ding ◽  
Shang-Bo Yu ◽  
Ji-Shi Wei ◽  
Huan-Ming Xiong
Keyword(s):  
Surface State ◽  
Carbon Dots ◽  
Light Emitting ◽  
Full Color

scholarly journals Microwave-Assisted Synthesis of Amikacin Modified N,S co-Doped Carbon Dots for Escherichia coli Detection

Chemosensors ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 61
Author(s):  
Fajar Amelia Rachmawati Putri ◽  
Mudasir Mudasir ◽  
Kinichi Morita ◽  
Suherman Suherman
Keyword(s):  
Escherichia Coli ◽  
Carbon Dots ◽  
Limit Of Detection ◽  
Low Cost ◽  
Microwave Assisted Synthesis ◽  
E Coli ◽  
Microwave Assisted ◽  
Average Size ◽  
Doped Carbon Dots ◽  
Co Doped

Fluorescent amikacin modified nitrogen, sulfur co-doped carbon dots (amikacin modified N,S-CDs) were synthesized by a facile and low-cost one-step microwave-assisted specifically for selective detection of Gram-negative bacteria Escherichia coli (E. coli). Amikacin is a semi-synthetic amino glycoside antibiotic and it was employed in this study to increase the fluorescence response of N,S-CDs by providing binding ligand towards E. coli. The effect of thiourea content as the source of nitrogen and sulfur dopants was investigated prior to the preparation of amikacin modified N,S-CDs. The formation of amikacin modified N,S-CDs were characterized by using Fourier transform infrared (FTIR), X-ray diffraction (XRD), Transmission electron microscope (TEM), UV-Vis spectrophotometer, and spectrofluorometer. Amikacin modified N,S-CDs was identified to be successfully synthesized from the wavenumber shift of the C=O stretching mode. Amikacin modified N,S-CDs were amorphous with an average size of 7 nm. Fluorescence spectra showed that the highest intensity was obtained at thiourea content of 50% and amikacin mass of 25 mg. By comparing fluorescence responses of all the investigated amikacin modified N,S-CDs, the limit of detection (LOD) was attained by 25 mg amikacin modified N,S-CDs at 1.526 cfu mL−1.

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