Invitro cell membrane stability study of methanol and aqueous extract of Begonia trichocarpa Dalzell

2020 ◽  
Vol 10 (2) ◽  
pp. 28-32
Author(s):  
Jeyasekhar M P ◽  
Jaslin Edward J
Keyword(s):  
Aqueous Extract ◽  
Blood Test ◽  
Methanol Extract ◽  
Cell Layer ◽  
Diclofenac Sodium ◽  
Stability Study ◽  
Cell Membrane Stability ◽  
Human Volunteers ◽  
Present Evaluation ◽  
Standard Medication

The point of present evaluation was to decide the layer balancing out impact of methanol and aqueous extract of Begonia trichocarpa on human red platelet (RBC) film. It was an invitro determination, where blood test was collected from the human volunteers and particular concentrations were made by weakening with supports. The cell suspensions were presented to 100 to 1000 μg/mL arrangement of methanol and aqueous extracts of Begonia trichocarpa. Cell film strength was broke down with various conditions like change in temperature and pH. Diclofenac sodium was utilized as standard medication. Every grouping of concentrates was performed in a triplicate way and the information were determined and introduced as Mean with standard deviation. Film settling exercises were altogether higher with cells which were presented to methanol concentrate of Begonia trichocarpa than fluid concentrate. The restraint of cell film break was focus dependent. This evaluation results demonstrated that the two concentrates has cell layer balancing out action on human RBC however methanol extract was powerful than fluid concentrate. Study report proposed that Begonia trichocarpa plant extracts may offer some helpful impacts in the management inflammatory mediators.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals Phytochemical Screening and TLC Profiling of Various Extracts of Reinwardtia indica

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Mehta Sonam ◽  
Rana Pawan Singh ◽  
Pooja Saklani
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Scientific Basis ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Biochemical Compound ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
The Himalaya

Reinwardtia indica, belongs to family Linaceae known as yellow flax or pyoli commonly found in the Himalaya. The plant has varied ethno medicinal importance such as aerial parts are used to prevent bleeding of cuts and as mouthwash; leaves are used in the treatment of paralysis and as natural antibiotic. Qualitative phytochemical screening of chloroform, acetone, ethanol, methanol and aqueous extracts was performed to explore scientific basis of ethno medicinal potential. It confirmed the presence of many phytochemicals like alkaloids, flavanoids, phenols, tannins, saponins, terpenoids, phlobatanins etc. in various extracts. Most of the phytochemicals were found in methanol and ethanol extracts. Thin Layer chromatography (TLC) of the acetone, methanol, chloroform and aqueous extract was performed for four important phytochemicals alkaloids, flavanoids, tannins and phenol. Flavanoids showed their presence in all extracts with one spot in each (Rf 0.8 for acetone, 0.918 for methanol, 0.816 for chloroform and 0.737 for aqueous extract). Alkaloids and tannins were found in acetone and methanol extract while phenol was present only in methanol extract (Rf 0.8). These findings provided the evidence that Reinwardtia indica is a potent source for some medicinally important phytochemicals and it justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active biochemical compound of medicinal utilities

scholarly journals Pharmacological Study on Wild Variety of Coccinia indica

2019 ◽  
pp. 396-400
Author(s):  
V. Pushpa Rani ◽  
A. Anitha Nancy ◽  
K. Shanmuga Priya
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Pharmacological Study ◽  
Dpph Assay ◽  
Abts Assay ◽  
Normal Variety ◽  
Effective Antioxidant ◽  
Wild Variety ◽  
Coccinia Indica ◽  
Anti Inflammatory

The current investigation dealt with pharmacological study of Coccinia indica (wild). For past several years, various medicinal plants were increasingly studied for the treatment of different ailments. The normal variety of C. indica was extensively used for home remedies and household purposes. But the wild variety of C. indica tastes bitter and showed good pharmacological activity. The current study revealed that ABTS assay was shown more scavenging activity than DPPH assay. The methanol extract of C. indica showed effective antioxidant activity than aqueous extract, but the property of anti inflammatory, aqueous extract of showed C. indica showed better anti inflammatory activity.

scholarly journals Comperative phyto toxicological and anti inflammatory effects of leaves extracts of holoptelea integrifolia

2018 ◽  
Vol 17 (2) ◽  
pp. 212-217
Author(s):  
Hina Yasin ◽  
Shaukat Khalid ◽  
Hina Abrar ◽  
Ghazala H Rizwani ◽  
Rehana Perveen ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Diclofenac Sodium ◽  
Medical Science ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
High Dose ◽  
Inhibition Activity ◽  
Standard Drug ◽  
Etoac Extract ◽  
Anti Inflammatory

Objective: Plants play valuable role in the new drug discovery and significantly used to treat different diseases. Nowadays scientists are investigating the therapeutically active phytochemical constituents that are safe and producing lesser side effect in comparative to other standard drugs.Methods: The plant Holoptelea integrifolia is medicinally important and this study was carried out to evaluate the anti inflammatory activity of aqueous extract of the leaves of Holoptelea integrifolia in male albino rats wistar stain treated with acetic acid to induced paw edema.Results: Result indicated the significant anti-inflammatory activity while compared with standard drug (diclofenac sodium).Brine shrimp bioassay (cytotoxicity), phytotoxicity, insecticidal and enzyme inhibition activity was performed in different extracts of the leaves of H. integrifolia. Results of brime shrimp bioassay indicating positive lethality at high dose in BuOH and H2O only. While the results of phytotoxicity in all crude extracts displayed mild phytotoxicity(46.3 µg/ml) in high concentrations (1000 µg/ml) except H2O extract showed no phytotoxicity. Result of insecticidal activity revealed that BuOH extract were found more effective against Rhyzoperthadominica, the EtOH extract expressed major while EtOAC extract showed mild activity against Callosobruchusanalis. Aqueous extract possessed no insecticidal activity.Conclusion: Results of Urease inhibition activity suggested that EtOAC and BuOH extracts of this plant expressed no activity while EtOH and H2O possessed mild inhibiting activity.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.212-217

scholarly journals Antioxidant properties of Ageratum conyzoides L. Asteraceae leaves

2018 ◽  
Vol 53 (4) ◽  
pp. 265-276 ◽  
Author(s):  
FO Adetuyi ◽  
KO Karigidi ◽  
ES Akintimehin ◽  
ON Adeyemo
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Erectile Dysfunction ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Tissue Homogenate ◽  
Total Phenolic ◽  
Ageratum Conyzoides

In vitro antioxidative and anti-lipid peroxidative properties of aqueous and methanol extracts of Ageratum conyzoides leaves were studied in controlling erectile dysfunction caused by oxidative stress. Methanol extract gave a significantly (P ˂ 0.05) higher content of total phenolic (61.4 mgGAE/g), total flavonoid (42.2 mgQE/g), ascorbic (10.1 mgAAE/100g) and phosphomolybdate (45.8 mgAAE/g) than the aqueous extract. The result showed that the extracts have high antioxidant activities. However, the methanol extract showed a higher DPPH and hydrogen peroxide scavenging activities over aqueous extract but the aqueous extract had a higher reducing power. The methanol extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ in rat pancreas and penile tissue homogenate exemplified by their least IC50 (94.21 μg/ml in pancreas) and (75.95 μg/mL in penile tissue) while in rat brain homogenate the aqueous extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ with least IC50 of 91.74 μg/mL. Hence, these extracts can be used as a potent natural antioxidant against free radicals and as a natural source of combating erectile dysfunction caused by oxidative stress. The extracts of Ageratum conyzoides leaves could be useful therapeutically as erectogenic agent.Bangladesh J. Sci. Ind. Res.53(4), 265-276, 2018

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
S. Sujitha
Keyword(s):  
Ascorbic Acid ◽  
Aqueous Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Apple Fruit ◽  
Fruit Pulp ◽  
Anticancer Activities ◽  
Anti Inflammatory ◽  
Limonia Acidissima

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.

scholarly journals Assessment of Paronychia Argentea Extraction on Kidney Stone by Using Calcium Oxalate Method

2020 ◽  
Vol 13 (4) ◽  
pp. 1745-1754
Author(s):  
Mousa K. Magharbeh ◽  
Tayel A. Al-Hujran ◽  
Saied M. I. Al-Dalaen ◽  
Abdul-Wahab R. Hamad
Keyword(s):  
Calcium Oxalate ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Crystal Size ◽  
Methanol Extract ◽  
Urinary Calculi ◽  
Ethyl Acetate Fraction ◽  
Ion Concentration ◽  
Calcium Oxalate Dihydrate ◽  
Oxalate Dihydrate

Urinary calculi are stones (urolithiasis) that can form anywhere in urinary tract outside of the kidneys and mostly composed of calcium oxalate and phosphate, additionally with elevated throughout the last two decades in the world. Chemical composition plays a major part in nephrolithiasis. Therefore, the high concentrations of lithogenic substances in urine enhance the crystallization method in urine tract system. The most kidney stones form from calcium oxalate, the present study was inspected the effect of the crude aqueous extract as well as the fractionated methanol extract (ethyl acetate, isopropanol, acetone and methanol residue) of paronychia argentea on the crystallization of calcium oxalate salts. The effect of aqueous extract and fractionated methanol extract on the size, number, type of calcium oxalate crystals. Paronychia argentea both the crude aqueous and the fractionated extract, especially ethyl acetate fraction have antiurolithic activity via reducing crystal size as well as activate the formation of calcium oxalate dihydrate (COD) crystals out from calcium oxalate monohydrate (COM) with increasing concentration of extract. The shifting of crystallization process to producing calcium oxalate dihydrate (COD) rather than oxalate monohydrate (COM) and the reducing the crystal size and calcium ion concentration, in addition to the diuretic action of extract plays an important role in controlling urolithiasis.

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