scholarly journals Preparation of yeast mediated semiconductor nanoparticles by Candida albicans and its bactericidal potential against Salmonella typhi and Staphylococcus aureus

2019 ◽  
Vol 10 (2) ◽  
pp. 861-864 ◽  
Author(s):  
Venkat Kumar S ◽  
Sowmya B ◽  
Geetha R ◽  
Karpagambigai S ◽  
Jacquline Rosy P ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Cadmium Sulfide ◽  
Salmonella Typhi ◽  
Cds Nanoparticles ◽  
X Ray Diffraction ◽  
Semiconductor Nanoparticle ◽  
Cds Nanoparticle ◽  
And Staphylococcus Aureus

Biosynthesis of nanoparticles using microorganism is widely used in biological applications due to its eco-friendly nature and lower cost. The present study aimed to investigate the yeast, Candida albicans for the synthesis of nano cadmium sulfide is a semiconductor nanoparticle. The yeast synthesized nanoparticles tested for its spectroscopic and microscopic characters. The peak at 420 nm identified by UV-Vis spectrophotometer confirms the Cadmium sulfide (CdS) nanoparticles synthesis preliminarily. Further, the nanoparticles were characterized using X-ray diffraction assay, scanning electron microscope, and elemental dispersive analysis. Finally, the synthesized Cadmium sulfide (CdS) nanoparticles were tested for its antibacterial activity against disease-causing pathogens such as Salmonella typhi and Staphylococcus aureus. The maximum zone of inhibition shows 15mm at the concentration of 100µl of CdS nanoparticle. Thus a promising antibacterial activity of yeast mediated synthesized Cadmium sulfide (CdS) nanoparticles was described.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

scholarly journals Melanin properties at the different stages towards life cycle of the fly Hermetia illucens

2017 ◽  
Vol 7 (4) ◽  
pp. 424-431 ◽  
Author(s):  
N. A. Ushakova ◽  
A. E. Dontsov ◽  
N. L. Sakina ◽  
E. S. Brodsky ◽  
I. A. Ratnikova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Life Cycle ◽  
Aspergillus Niger ◽  
Lauric Acid ◽  
Hermetia Illucens ◽  
Wide Range ◽  
And Staphylococcus Aureus ◽  
Test Cultures

<p>Eumelanin type pigments are synthesized at all the stages of the life cycle of the fly Hermetia illucens: in the larvae, pre-pupae,<br />pupae and adult flies (dead flies). The greatest content of melanin was recorded in the cuticles. Melanin was present not only<br />in the cuticle, hence it remained in the cuticle after the emergence of the adult fly. It was also found in the insect body in a<br />complex with lipids. In pupae, it is mostly lauric acid that was associated with melanin. Its proportion in the melanin-chitosan<br />complex was 80%. The isolated melanin-chitosan complex of adult flies showed a wide range of antibacterial activity, inhibiting<br />the growth of 21 out of the 25 of the test cultures. The melanin-chitosan complex of empty pupal membranes and alcohol<br />suspension of pupal melanin inhibited twice as smaller number of test cultures and the above activity was absolutely in the<br />pupal chitosan. The largest zone of growth inhibition was recorded with respect to Aspergillus niger, Candida albicans,<br />salmonella, and Staphylococcus aureus. An alcohol suspension of pupal melanin inhibited the growth of 10 test cultures. In this<br />case the greatest activity was shown in relation to Mycobacterium B5 and Acinetobacter sp. 1182.</p>

Ferrocene-Based Antibacterial Agents: Synthesis, Characterization, and Biological Evaluation of Metal (II) Complexes with Glycine-Derived Compounds

2011 ◽  
Vol 339 ◽  
pp. 317-320 ◽  
Author(s):  
Yu Ting Liu ◽  
Xiao Ming Sun ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Salmonella Typhi ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

A series of Ferrocene Schiff bases derived from glycine and their cobalt (II), copper (II), nickel (II), and zinc (II) metal complexes have been synthesized and characterized by IR, 1H NMR, MS and elemental analysis, the results conformed well with expected structures. The synthesized ligands, along with their metal (II) complexes, were screened in vitro for their antibacterial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that the metal (II) complexes are more potent in antibacterial activity against one or more species in comparison with those of uncomplexed ligands.

scholarly journals New Isocoumarin Analogues from the Marine-Derived Fungus Paraphoma sp. CUGBMF180003

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 313
Author(s):  
Xiuli Xu ◽  
Jiangpeng Li ◽  
Kai Zhang ◽  
Shangzhu Wei ◽  
Rui Lin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Secondary Metabolites ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Chemical Structures ◽  
Nmr Techniques ◽  
And Staphylococcus Aureus

Nine new secondary metabolites, including six isocoumarin analogues, 7-hydroxyoospolactone (1), 7-methoxyoospolactone (2), 7-methoxy-9-hydroxyoospolactone (3), 10-acetoxy-9-hydroxyoospolactone (4), 6-dehydroxysescandelin (5), parapholactone (6), and three compounds with a rare skeleton of isocoumarin coupled with phenylethylamine, namely paraphamide A (12), paraphamide B (13), and paraphamide C (14), together with five known compounds, oospolactone (7), 8-O-methyloospolactone (8), 10-hydroxyoospolactone (9), 9,10-dihydroxyoospolactone (10), and oospoglycol (11), were isolated and identified from the marine-derived fungus Paraphoma sp. CUGBMF180003. Their chemical structures were determined using spectroscopic data, including HRESIMS and 1D and 2D NMR techniques. Furthermore, the stereogenic carbons in 5 and 14 were determined by comparing the experimental and calculated electronic circular dichroism (ECD) spectra. The carbon skeleton of 12–14 was identified as the first example of isocoumarin coupled with phenylethylamine derivatives. All of these compounds were examined for antimicrobial activities against Candida albicans and Staphylococcus aureus. Both 1 and 6 showed antibacterial activity against S. aureus with MIC values of 12.5 μg/mL.

scholarly journals Anion Induced Synthesis, Structural Characterization and Antibacterial Activity of Zinc(II) Complexes Derived from 5-Bromo-2-((2-(diethylamino)ethylimino)methyl) phenol

2021 ◽  
Vol 68 (3) ◽  
pp. 693-699
Author(s):  
Huan-Yu Liu ◽  
Xiang Gan ◽  
Jin-Yan Ding ◽  
Zhi-Tao Li ◽  
Qiao Chen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Tetrahedral Coordination ◽  
X Ray Diffraction ◽  
X Ray ◽  
Trigonal Bipyramidal ◽  
Vis Spectroscopy ◽  
Zinc Salts

By changing the anions of zinc salts, three different zinc(II) complexes, [Zn2(HL)2(NCS)4]·2CH3OH (1), [Zn2L(μ2-η1:η1-CH3COO)2(NCS)] (2) and [Zn(HL)I2]·CH3OH (3), where L = 5-bromo-2-((2-(diethylamino)ethylimino)methyl)phenolate, HL = 5-bromo-2-((2-(diethylammonio)ethylimino)methyl)phenolate, have been synthesized and characterized by IR and UV-Vis spectroscopy, as well as single-crystal X-ray diffraction. X-ray analysis indicates that the Zn atoms in the complexes are in trigonal bipyramidal, square pyramidal and tetrahedral coordination. The anions of the zinc salts lead to the formation of different structures of the complexes. Antibacterial activity of the complexes against Staphylococcus aureus, Escherichia coli, Klebsielle pneumoniae and Candida albicans strains was studied.

scholarly journals Antibacterial Activity of Haematococcus pluvialis Crude Astaxanthin Extract

2021 ◽  
Vol 11 (2-S) ◽  
pp. 28-30
Author(s):  
Ashaq Hussain Rather ◽  
Surendra Singh ◽  
Sameer Choudhary
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Haematococcus Pluvialis ◽  
Salmonella Typhi ◽  
Vibrio Cholera ◽  
Potential Source ◽  
Crude Extracts ◽  
And Staphylococcus Aureus

H. pluvialis is the potential source of natural astaxanthin, which is considered as super antioxidant. In the present investigation, astaxanthin was extracted from the encysted cells of H. pluvialis with acetone, methanol, DMSO and hexane, the crude extracts were tested for four strain (Escherichia coli, Salmonella typhi, Vibrio cholera and Staphylococcus aureus)of bacteria for the antibacterial activity. Highest antibacterial activity was observed as 10.2 ±0.20 mm extracted with acetone on Escherichia coli while as least antibacterial activity was found as6.1±0.0 mm extracted with hexane on Vibrio cholera. Keywords: Haematoccoccus pluvialis; Astaxanthin; Antibacterial.

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI ISOLAT BAKTERI YANG BERASOSIASI DENGAN SPONS Callyspongia aerizusa SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 380
Author(s):  
Angelika Liempepas ◽  
Widya A. Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis ◽  
Health Sector ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Agar Disc ◽  
And Staphylococcus Aureus

ABSTRACT               Sponge Callyspongia aerizusa contain potential bioactive compound that can be utilized in the health sector. Extract of sea sponge Callyspongia aerizusa, can hamper the growth of Salmonella typhi bacteria, Streptococcus pyogenes, Shigella and Staphylococcus epidermidis. The aim of this study was to test the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and identify the type of symbionic bacteria of Callyspongia aerizusa sponge based on their physiological and biochemical characteristics. The method of testing the antibacterial activity was agar diffusion method (Kirby and Baurer diffusion disc). There were three bacterial isolates namely T1, T2, and T3 isolates. The result showed that T1, T2, and T3bacterial isolates had antibacterial activity against Escherichia coli and Staphylococcus aureus test bacteria. Based on the biochemical test, T2bacterial isolates were identified as Bronchothrix bacteria and T1and T3 bacterial identified as Desulfotomaculum. Keywords: Callyspongia aerizusa, Antibacterial activity, symbiont bacteria, Biochemical Identification   ABSTRAKSpons Callyspongia aerizusa memiliki kandungan senyawa bioaktif potensial yang dapat dimanfaatkan dibidang kesehatan. Ekstrak spons laut Callyspongia aerizusa dapat menghambat pertumbuhan bakteri Salmonella typhi, Streptococcus pyogenes, Shigella dan Staphylococcus epidermidis. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari bakteri simbion spons Callyspongia aerizusa terhadap bakteri Escherichia coli dan Staphylococcuc aureus dan mengidentifikasi jenis bakteri simbion spons Callyspongia aerizusa berdasarkan karakteristik fisiologis dan biokimianya. Metode pengujian aktivitas antibakteri yang digunakan yaitu metode difusi agar (disc diffusion Kirby and Baurer). Terdapat tiga isolat bakteri yaitu isolat T1, T2, dan T3. Hasil penelitian menunjukan bahwa isolat bakteri T1, T2, dan T3 memiliki aktivitas antibakteri terhadap bakteri uji Escherichia coli dan Staphylococcuc aureus. Berdasarkan uji biokima, isolat bakteri T2 diduga sebagai bakteri Brochothrix dan  isolat bakteri T1 dan T3 diduga sebagai bakteri Desulfotomaculum.Kata kunci: Callyspongia aerizusa, Aktivitas antibakteri, Bakteri simbion, Identifikasi Biokimia

scholarly journals Potential of invasive alien species Clidemia hirta as antibacterial against Salmonella typhi and Staphylococcus aureus

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Mentari Putri Pratami ◽  
Miftahul Huda Fendiyanto ◽  
Rizky Dwi Satrio ◽  
I Dewa Ketut Kerta Widana ◽  
Isna Arofatun Nikmah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Alien Species ◽  
Water Content ◽  
Aqueous Extract ◽  
Invasive Alien Species ◽  
Morphological Characteristics ◽  
Salmonella Typhi ◽  
Screening Tests ◽  
And Staphylococcus Aureus

Abstract. Pratami MP, Fendiyanto MH, Satrio RD, Widana IDKK, Nikmah IA, Sari NIP, Awwanah M, Farah N, Darmadi D. 2021. Potential of invasive alien species Clidemia hirta as antibacterial against Salmonella typhi and Staphylococcus aureus. Biodiversitas 22: 3363-3369. Clidemia hirta D. Don is an invasive alien species (IAS) that is a threat to biodiversity in tropical country particularly Indonesia, and remains underutilized to date. Conversely, prevalence of typhus in Indonesia is generally higher every year. Thus, the aim of this study was to detect phytochemicals in ethanolic and aqueous extracts of C. hirta and their antibacterial activity against Salmonella typhi and Staphylococcus aureus. The research methods included sample identification, generating the simplicia, determination of water content, extraction, phytochemical screening tests, and antibacterial activity tests. Identification was made based on morphological characteristics. The water content in the dried powder of simplicia was 12.26 ± 0.39%. Phytochemical results showed that 70% ethanol extract of C. hirta contained flavonoids, saponins, tannins, and triterpenoids compounds. In addition, aqueous extract of C. hirta showed positive results on flavonoids, saponins, tannins, and steroids tests. Antibacterial activity results showed that ethanolic extracts of C. hirta inhibited S. typhi and S. aureus at all concentrations, while aqueous extract inhibited bacterial growth in only 12.5% ??and 25% concentrations. These findings indicate that C. hirta has antibacterial activity that inhibits S. typhi and S. aureus. This information can be used for adding preliminary data to metabolite interest researchers, i.e., biologists and biotechnologists in the future.

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