scholarly journals Phytochemical screening, antioxidant and antilithiasis activities of Juncus acutus

2020 ◽  
Vol 11 (3) ◽  
pp. 3075-3083
Author(s):  
Zineb Aliat ◽  
Lamyae Yachi ◽  
Houda Attjioui ◽  
Amine Cheikh ◽  
Miloud EL Karbane ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Screening ◽  
Uric Acid Stones ◽  
Juncus Acutus ◽  
Cystine Stones

The objective of this work was to perform a phytochemical screening of Juncus acutus and to evaluate its antioxidant activity and antilithiasis activity. Phytochemical screening has demonstrated the presence of catechin, tannins and alkaloids in the various extracts studied. The antioxidant activity of the methanolic and ethyl acetate extracts by ultrasound and maceration was evaluated by the method of DPPH, FRAP and phosphomolybdate. The ethyl acetate extract by ultrasound, the methanolic extract by maceration and ultrasound respectively showed IC 50 with DPPH of 1,449mg / ml, 1,535mg / ml and 4,771mg / ml and a reducing power was observed at concentrations of 419,561mgBHA /100g, 207,143mgBHA /100g and 142,20mgBHA /100g by FRAP and at concentrations of 839,470mgAAE/100g, 283,260mgAAE/100g and 141,018mgAAE/100g by the phosphomolybdate test. The determination of the total polyphenols of the ethyl acetate extract by ultrasound, the methanolic extract by maceration and by ultrasound presented respectively contents of 2,428 mg GAE / g, 1,960mg GAE / g and 1,172mg GAE / g, likewise, the flavonoid assay showed respectively concentrations of 14,469mg EQ/g, 6,466 mg EQ/g and 3,143 mg EQ/g. Antilithiasis activity was evaluated on carbapatite, cystine and uric acid stones. The mass loss of uric acid, carbapatite and cystine stones was respectively 42%, 7% and 16%.

Preliminary Phytochemical screening of Persicaria sagittata: Future prospective for kidney stone

2021 ◽  
pp. 4367-4370
Author(s):  
Ankita Sharma ◽  
Ishita Ishita ◽  
Inder Kumar ◽  
Kritika Verma ◽  
Bhumika Thakur ◽  
...  
Keyword(s):  
Uric Acid ◽  
Medicinal Plant ◽  
Kidney Stone ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Secondary Product ◽  
Standard Methods ◽  
Active Constituents ◽  
Uric Acid Stones

Medicinal plants have bioactive compounds, which are used to treatments of various diseases. In the present study, Persicaria sagittata medicinal plant was used for the preliminary phytochemical screening. Methanol and petroleum ether were used as a solvent for obtaining the extraction of the plant. Both the extracts were further study for qualitative phytochemical screening using standard methods. Phytochemical screening shows that methanolic extract possesses the presence of tannins, flavonoids, and vitamin A as tannins and flavonoids are helpful for the uric acid stones. The study reveals that medicinal plant provides a basis of its use in medicine, improve to further drugs in the pharmaceutical area, and contains different biologically active constituents, and the secondary product is valuable of further analysis.

Phenolic Compounds and Antioxidant Activity of Rice Straw Extract

2017 ◽  
Vol 64 ◽  
pp. 1-9 ◽  
Author(s):  
Abdelnaser Abdelghany Elzaawely ◽  
Hanafey F. Maswada ◽  
M.E.A. El-Sayed ◽  
Mohamed E. Ahmed
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Rice Straw ◽  
Phenolic Acids ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Lipid Peroxidation Inhibition ◽  
High Level

Agricultural wastes cause a serious environmental problem in Egypt. Utilization of these wastes by an environmentally friendly way is a very important issue. The objective of this study was to utilize rice straw into high-value products. Antioxidant activity, total soluble phenols, and flavonoids were evaluated in ethyl acetate extract prepared from rice straw after alkaline hydrolysis. Total phenols were found to be 221.6 mg gallic acid equivalents (GAE), while total flavonoids were 4.9 mg rutin equivalents (RE). The extract exhibited strong antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and its IC50 value was 0.4 mg/ml. Furthermore, the ethyl acetate extract possessed high antioxidant activity assayed by β-carotene bleaching method and its value of lipid peroxidation inhibition (LPI) was 75.4%. It also exhibited high reducing power and its IC50 value was equal to 0.06 mg/ml. HPLC analysis indicated that this extract contained seven phenolic acids including; protocatechuic, caffeic, syringic, p-coumaric, ferulic, rosmarinic and cinnamic, in addition to two flavonoids; qurecetin and kaempferol. Results indicated that ferulic and p-coumaric acids were the major soluble phenolic acids in rice straw, and their concentrations were 3.9 and 2.9 mg/g DW. It can be summarized that the strong antioxidant activity of ethyl acetate extract, prepared from rice straw, was highly correlated with its high level of phenolic compounds.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF HABENARIA PECTINATA

2020 ◽  
pp. 68-71
Author(s):  
CHARANJIT KAUR ◽  
RAJESH KUMAR ◽  
GURVINDER SINGH ◽  
CHANDER MOHAN
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Anti Oxidant

Objective: This study was designed to evaluate the antioxidative potential of tubers of Habenaria pectinata. Methods: The tubers of H. pectinata were extracted using hexane, ethyl acetate, methanol, and water as solvents. The anti-oxidant potential of extracts was evaluated using free radical scavenging and reducing power assays. The most active methanolic extract was then fractionated into four fractions using the above-mentioned solvents. Results: The phenol and flavonoid content was found to be maximum in the methanol extracts. All the extracts and fractions showed significant levels of antioxidant activity except hexane extract. Conclusion: The tubers of H. pectinata were found to possess a significant antioxidant potential and can be explored further for isolation and preclinical investigation for the ailment of various diseased states and disorders.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

2019 ◽  
pp. 55-57
Author(s):  
ACE BAEHAKI ◽  
SHANTI DWITA LESTARI ◽  
NORYATI SIREGAR
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Power Method ◽  
Activity Assessment ◽  
Sample Extraction ◽  
Phytochemical Compounds

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method. Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171. Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

scholarly journals Phytochemical Profile And Antioxidant Activity Of Guazuma ulmifolia Leaves Extracts Using Different Solvent Extraction

2020 ◽  
pp. 171
Author(s):  
Mohamad Rafi ◽  
Nadya Meitary ◽  
Dewi Anggraini Septaningsih ◽  
Maria Bintang
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolics ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
The Body ◽  
Ftir Spectrum ◽  
Dry Powder ◽  
Guazuma Ulmifolia ◽  
Phytochemical Profile

Guazuma ulmifolia is one of the common tropical plants that has long been used as a traditional medicine to reduce body weight as slimming herbs and to lower the cholesterol in the body. The leaves of G. ulmifolia contain phenolics compound such as flavonoids and tannin that contributes to its biological activities. In this research, we determined the content of total phenolics, flavonoids, tannins, antioxidant activity, and FTIR spectrum profile from four extracts of G. ulmifolia leaves. The extraction method used in this study was stepwise maceration with different solvents n-hexane, ethyl acetate, ethanol, and water. The content of total phenolics and flavonoids were found high in ethanol extract of G. ulmifolia leaves with 35.42 and 44.85 mg QE/g dry powder, respectively. The highest content of total tannins was obtained in ethyl acetate extract of G. ulmifolia leaves as 0.55%. Antioxidant activity from four extracts of G. ulmifolia leaves was measured using DPPH, CUPRAC, and reducing power method. The highest antioxidant activity for DPPH and CUPRAC method was obtained in ethyl acetate extract with the antioxidant capacity as 55.47 and 98.17 µmol trolox/g dry powder, respectively. While using reducing power assay gives the capacity 176.75 µmol trolox/g dry powder in the water extract. The pattern of FTIR spectra from four extracts of G. ulmifolia leaves gives the distinct characteristics of each spectrum profile. Principal component analysis using FTIR spectrum shows good clustering for each extract with 94% data variability (PC1= 77% and PC2 = 17%). It can be concluded that each extract of G. ulmifolia leaves gives a distinct phytochemical profile (phenolics content, flavonoids, tannins, and FTIR spectrum) that contributes to the antioxidant activity. This antioxidant activity mainly influenced by the concentration of phenolic compounds, which is known to have antioxidant properties.

scholarly journals Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants

2016 ◽  
Vol 70 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Gorica Ðelic ◽  
Miroslav Vrvic
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Dpph Scavenging

The antioxidant activity, total phenolic and flavonoid content of ethanolic, acetone and ethyl acetate extracts of Ruscus hypoglossum L. and Ruscus aculeatus L. (aerial parts) from Serbia were investigated in this paper. The best total antioxidant capacity (23.329 ?g AA g-1) and the highest DPPH scavenging activity (IC50 = 182.54 ?g mL-1) were found in acetone and ethyl acetate extract of R. aculeatus L. Ethanolic extract of R. hypoglossum L. showed the highest ABTS radical cation scavenging activity (IC50 = 3.04 ?g mL-1) as well as reducing power (IC50 = 0.143 ?g mL-1). The best inhibitory activity against lipid peroxidation (IC50 =651 ?g mL-1) and the best ferrous ion chelating ability (IC50 = 110 ?g mL-1) were found in acetone and ethyl acetate extract of R. hypoglossum L. The highest total phenolic (8.569 mg GAE g-1) and flavonoid content (0.136 mg RU g-1) were found in ethanolic and acetone extract of R. hypoglossum L. and R. aculeatus L, respectively.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF THE METHANOLIC EXTRACT OF LINDERNIA RUELLIOIDES (COLSM.) PENNELL

2020 ◽  
pp. 141-146
Author(s):  
IMTILEMLA A ◽  
VICKY BAREH ◽  
SAMIA BEGAM BARBHUIYA ◽  
LALZIKPUII SAILO
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Fluorescence Analysis ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Reducing Power Assay ◽  
Dpph Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective of the study was collection of plant materials, Extraction of phytoconstituents using a different solvent, to carry out preliminary phytochemical screening of different extracted solvent, to perform fluorescence analysis, to estimate the proximate composition of the leaves Lindernia ruellioides (Colsm.) Pennell, and to determine the presence of in vitro anti-oxidant of the methanolic extract of the plant. Method: Preliminary phytochemical screening of the methanolic extract of Lindernia ruellioides (Colsm.) Pennell, estimation of proximate composition of the leaves, fluorescence analysis, total phenolic content, total flavonoids content, and in vitro antioxidant activity of the methanol extract (DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity). Results: The result of phytochemical screening of methanolic extract of Lindernia ruellioides (Colsm.) Pennell contents the presence of amino acid, flavonoids, tannins, steroids, and triterpenoids. The moisture content and Ash value were found to be appropriate and the in vitro antioxidant activity of the methanolic extract showed potential antioxidant activity in terms of DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity. Conclusion: The work presented here suggests that the methanolic extract of Lindernia ruellioides (Colsm.) Pennell possesses potential antioxidant activity.

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