“Pharmaco-chemical Characterization and Evaluation of In Vitro Antioxidant and Antidiabetic Activity of Ethanolic Flower Extract of Clerodendrum paniculatum”

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.

Download Full-text

Evaluation of in vitro antioxidant, antidiabetic activities and GC-MS analysis from Amomum nilgiricum leaf extract

Plant Science Today ◽

10.14719/pst.2020.7.4.857 ◽

2020 ◽

Vol 7 (4) ◽

Author(s):

Narasimhamurthy Konappa ◽

Udayashankar C Arakere ◽

Soumya Krishnamurthy ◽

Kusuma Chathrapalya Gangadharaiah ◽

Vinod Gubbiveeranna ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Yeast Cells ◽

Mass Spectrometry Analysis ◽

Antidiabetic Activity ◽

Gas Chromatography Mass Spectrometry ◽

Methanol Fraction ◽

Ms Analysis ◽

Ic50 Value

In the present study, hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic efficacy through in vitro ?-amylase and ?-glucosidase assays, DPPH and H2O2 scavenging activities, followed by estimation of total phenol, total flavonoids and gas chromatography-mass spectrometry analysis. In the present study, a significant amounts of total phenolics (79.92±1.58 mg/g) and flavonoids (21.74± 0.89 mg/g) were showed from Ethyl acetae faction. Ethyl acetate fraction showed maximum inhibition of DPPH radicals (82.31±2.33%) with IC50 value of 52 µg/ml and H2O2 scavenging activity (97.62±2.89%) with IC50 value of 78.57 µg/ml concentrations. The ethyl acetate fraction was revealed maximum ?-amylase inhibition (77.23± 3.21%) with IC50 value 76.53 µg/ml. The ethyl acetate fraction recorded maximum ?-glucosidase inhibition (85.36±2.58%) with IC50 value 79.54 µg/ml. Ethyl acetate fraction exhibited maximum inhibitory activity of glucose movement into outer solution across dialysis membrane at 250 µg/ml as compared to the control. The ethyl acetate fraction revealed maximum insulin secretory activity (130.5±3.66%) in RIN-m5F cells. Methanol fraction recorded maximum glucose uptake percent in yeast cells (67.08±1.68%) when compared to standard metronidazole (68.06±0.73%). The GC-MS analysis of ethyl acetate fraction was recorded the presence of six phytochemical constituents. This study scientifically validates the antidiabetic activity of A. nilgiricum. Hence, in view of its comparative hypoglycemic strength, it can work as a valuable healing agent in treating diabetes.

Download Full-text

Chemical Characterization and Antidiabetic Activity of Essential Oils from Pelargonium graveolens Leaves

ARO-The Scientific Journal of Koya University ◽

10.14500/aro.10791 ◽

2021 ◽

Vol 9 (1) ◽

pp. 109-113

Author(s):

Javed Ahamad ◽

Subasini Uthirapathy

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Chemical Constituents ◽

Chemical Characterization ◽

Study Finding ◽

Antidiabetic Activity ◽

Chemical Components ◽

Diabetic Patients ◽

Pelargonium Graveolens

Pelargonium graveolens (Geranium) is a source of the finest quality of fragrance and its essential oils are used as antibacterial, and antifungal agents. The aim of the current research is to determine chemical constituents in the essential oil of P. graveolens by GC-MS and evaluate its antidiabetic activity via α-glucosidase inhibition assay. The chemical composition of P. graveolens essential oil was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. GC-MS analysis determines 36 chemical components in the essential oil of P. graveolens leaves, and citronellyl isovalerate (10.41 %), menthol (9.61 %), linalool (8.63 %), p-menthone (6.31 %), and geranyl tiglate (4.99 %) were recorded as major constituents. The essential oil of P. graveolens leaves showed concentration dependant inhibition of α-glucosidase enzyme ranging from 28.13±1.41 to 74.24±2.53 µg/mL for concentration ranging from 31.25 to 1000 µg/mL. The IC50 values for of P. graveolens and acarbose were found as 93.72±4.76 and 80.4±2.17 µg/mL, respectively against the α-glucosidase enzyme. The study finding explores the chemical components of P. graveolens growing in the Iraqi Kurdistan region and scientifically supported its possible use in diabetic patients for controlling postprandial hyperglycemia.

Download Full-text

SCREENING OF BIOACTIVE COMPONENTS BY GC-MS ANALYSIS AND THE STUDY ON IN VITRO ANTICANCER EFFICACY OF MICROPORUS AFFINIS (BLUME & T. NEES) KUNT.

International Research Journal of Pharmacy ◽

10.7897/2230-8407.1112104 ◽

2020 ◽

Vol 11 (12) ◽

pp. 31-34

Author(s):

S Aneesh ◽

J E Thoppil

Keyword(s):

Biological Sciences ◽

Bioactive Compounds ◽

Chemical Characterization ◽

Bioactive Components ◽

Anticancer Activities ◽

Anticancer Efficacy ◽

Ms Analysis ◽

Ethidium Bromide Staining ◽

Leading Compounds

Natural compounds with biological activity are normally present in plants, mushrooms and their natural sources. Applied mycology is one of the most stimulating and rapidly evolving areas of the biological sciences. Hence the present study focussed on exploring Microporus affinis (Blume & T. Nees) Kunt., the least explored and edible bracket fungus. Chemical characterization by GC-MS analysis resulted in the presence of 47 bioactive compounds. 9, 12- Octadecadienoic acid (Z,Z)- methyl ester, Ergosterol, Monolinolein, Thiacremonone, Stellasterol, n- Hexadecanoic acid, Ribitol, Maltol etc., were the leading compounds. Because of the presence of various bioactive compounds which have been already reported to possess antitumor, antioxidant and anticancer activities, M. affinis extract has been tested for in vitro anticancer efficacy on DLD1 cell lines (cultured in DMEM medium) using MTT assay. It resulted in the decrease of percentage of viability as the increase in concentration of the extract. Apoptosis was determined by using Acridine orange and Ethidium bromide staining. Thus, the taxa, M. affinis can be recommended for further anticancer assays for validation.

Download Full-text

In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Canthium coromandelicum Leaves

European Journal of Medicinal Plants ◽

10.9734/ejmp/2020/v31i430225 ◽

2020 ◽

pp. 12-18

Author(s):

S. Thiripura Salini ◽

S. Shankar

Keyword(s):

Diabetic Complications ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Antidiabetic Activity ◽

Dpph Radical ◽

Dpph Assay ◽

Ic50 Value ◽

Ic50 Values ◽

In Vitro Antioxidant Activity

Oxidative stress plays a major role in diabetic complications. The study aim was to investigate the in-vitro antidiabetic and antioxidant activities of methanolic extract of Canthium coromandelicum leaves. The plant material was extracted with methanol and the methanolic extract was screened for in-vitro antioxidant activity using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) assay. The efficiency of the antidiabetic activity of the plant extract was evaluated against α-amylase and α-glucosidase digestive enzymes. The study revealed that the C. coromandelicum extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 31.52 ± 0.42 and 41.49 ± 0.28 µg/mL respectively and compared with standard acarbose drug. The extract efficiently scavenging DPPH radical with IC50 values of 65.46 ± 0.50 µg/ml. Therefore, the extract could be a promising therapeutic in management of diabetic complications.

Download Full-text

Mechanochemical Synthesis, in vitro Evaluation and Molecular Docking Studies of 4-Amino-2-arylamino-5-(benzofuran-2-oyl)thiazoles as Antidiabetic Agents

Letters in Organic Chemistry ◽

10.2174/1570178615666180815124425 ◽

2019 ◽

Vol 16 (7) ◽

pp. 560-568

Author(s):

Vijayan R. Akhila ◽

Maheswari R. Priya ◽

Daisy R. Sherin ◽

Girija K. Krishnapriya ◽

Sreerekha V. Keerthi ◽

...

Keyword(s):

Molecular Docking ◽

Mechanochemical Synthesis ◽

Active Sites ◽

Docking Studies ◽

Binding Energies ◽

Antidiabetic Activity ◽

Antidiabetic Agents ◽

Molecular Docking Studies ◽

Ic50 Value

The synthesis of 4-amino-2-arylamino-5-(benzofuran-2-oyl)thiazoles 4a-h, as example of 2,4-diaminothiazole-benzofuran hybrids and an evaluation of their antidiabetic activity, by in vitro and computational methods, are reported. The synthesis of these diaminothiazoles was achieved mechano chemically by a rapid solvent-less method. Their antidiabetic activity was assessed by α-glucosidase and α-amylase inhibition assays. The, IC50 value for α-glucosidase inhibition by 4-amino-5- (benzofuran-2-oyl)-2-(4-methoxyphenylamino)thiazole (4d) was found to be 20.04 µM and the IC50 value for α-amylase inhibition, 195.03 µM, whereas the corresponding values for reference acarbose were 53.38 µM and 502.03 µM, respectively. Molecular docking studies at the active sites of α- glucosidase and α-amylase showed that among the diaminothiazoles 4a-h now studied, 4-amino-5- (benzofuran-2-oyl)-2-(4-methoxyphenylamino)thiazole (4d) has the highest D-scores of -8.63 and -8.08 for α-glucosidase and for α-amylase, with binding energies -47.76 and -19.73 kcal/mol, respectively.

Download Full-text

PHYTOCHEMICAL ANALYSIS AND IN VITRO STUDIES ON ANTIBACTERIAL, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES USING CASUARINA EQUISETIFOLIA BARK EXTRACTS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i1.22188 ◽

2018 ◽

Vol 10 (1) ◽

pp. 118

Author(s):

Saranya V. T. K. ◽

S. Uma Gowrie

Keyword(s):

Secondary Metabolites ◽

Tree Species ◽

Forest Tree ◽

Root Extract ◽

Dependent Manner ◽

Casuarina Equisetifolia ◽

Forest Tree Species ◽

Ms Analysis ◽

Anti Inflammatory

Objective: Casuarina equisetifolia is an important multipurpose exotic forest tree species widely cultivated in the coastal regions of Tamil Nadu that serves as a warehouse of essential secondary metabolites. Identification of these bioactive compounds in this forest tree species might lead to the discovery and development of a new drug to treat various diseases. Methods: The present study was carried out with an objective to analyse the phytochemicals qualitatively and quantitatively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to evaluate the presence of various volatile compounds. An in vitro antibacterial, antioxidant and anti-inflammatory properties of aqueous and organic solvents of C. equisetifolia bark was studied.Results: The preliminary qualitative screening revealed the presence of alkaloids, glycosides, carbohydrates, proteins, flavonoids, phenols, terpenoids, and tannins. The quantitative analysis revealed the presence of maximum phenols (71.2±0.51 mg/g), flavonoids (35.12±0.34 mg/g), tannins (77.59±0.21) and terpenoids (6%) in methanolic root extract with respective standards. Several peaks were obtained in the GC-MS analysis which indicates the presence of different secondary metabolites. Antibacterial activity showed a maximum zone of inhibition against Escherichia coli (23±0.24 mm) and Proteus vulgaris (23±0.32 mm). The antioxidant potential of various extracts was compared with the standard ascorbic acid. Anti-inflammatory activity was compared with standard diclofenac sodium and the extract showed activities significantly in a dose-dependent manner. Conclusion: From this study, it is revealed that C. equisetifolia bark extract possesses efficient antibacterial property, the potential in scavenging free radical, effective antioxidant, powerful anti-inflammatory source that can be employed in the development of a novel drug to treat various diseases.

Download Full-text

STUDIES ON IN VITRO ANTIDIABETIC ACTIVITIES OF HOPEA PONGA AND VITEX LEUCOXYLON

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.16280 ◽

2017 ◽

Vol 9 (2) ◽

pp. 263 ◽

Cited By ~ 1

Author(s):

Arun Kashivishwanath Shettar ◽

Ankala Basappa Vedamurthy

Keyword(s):

Glucose Uptake ◽

Aqueous Extract ◽

Yeast Cells ◽

Antidiabetic Activity ◽

Significant Activity ◽

Soxhlet Apparatus ◽

Ic50 Value ◽

Uptake Assay ◽

Glucose Uptake Assay

Objective: Evaluating antidiabetic property of Hopea ponga and Vitex leucoxylon extracts by using in vitro assays.Methods: The exhaustive serial extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for antidiabetic activity by employing standard in vitro techniques (α-amylase and glucose uptake assay using yeast model in which the effects of extracts on α-amylase and glucose uptake was tested by considering the percentage of inhibition of α-amylase and increase in glucose uptake in yeast cells).Results: In vitro antidiabetic studies show that in case of Hopea ponga methanol extract showed comparable antidiabetic activity with percentage of α-amylase inhibition 51.7925±0.92794 % and with IC50 value 96.53 µg and it was less on comparison with standard i.e. 71.0907±0.67796% with IC50 value 70.33 µg and in case of glucose uptake assay aqueous extract showed higher activity over all remaining extracts with percentage of inhibition 49.8100±0.62476% and with IC50 value 250.95 µg. whereas in case of Vitex leucoxylon aqueous extract exhibited significant activity in both performed assays i. e α-amylase inhibition and glucose uptake assay with percentage 54.6147±0.46397% and 57.1337±0.44201% respectively when compared to other solvent extracts.Conclusion: Results confirm that aqueous extract of Vitex leucoxylon exhibited highest antidiabetic activity among all extracts. Additional studies are needed for purification, characterization and structural elucidation of bioactive compounds from aqueous extract and also confirm its antidiabetic property by in vivo studies. The present study provides scientific evidence that the leaves of Hopea ponga and Vitex leucoxylon possess anti-diabetic efficacy. Thus, considering its relative antidiabetic potency, these extracts are the useful therapeutic agents for treating and management of diabetes.

Download Full-text

Antidiabetic‎ Activity of Terfezia Claveryi; An In Vitro and In Vivo Study

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1680 ◽

2019 ◽

Vol 12 (2) ◽

pp. 603-608

Author(s):

Anas AlAhmed ◽

Hany Ezzat Khalil

Keyword(s):

Methanol Extract ◽

Positive Control ◽

Alpha Tocopherol ◽

Antidiabetic Activity ◽

Inhibition Assay ◽

Ic50 Value ◽

Enzymatic Glycosylation ◽

Terfezia Claveryi

The main objective of current study was to investigate the in vitro and in vivo antidiabetic activity of Terfezia claveryi methanol extract. In vitro antidiabetic assays such as inhibition of α-amylase enzyme and non-enzymatic glycosylation of hemoglobin were carried out. The results of α- amylase inhibition assay revealed that the inhibitory activity (IC50) of Terfezia claveryi methanol extract (‎38.7µg/ml) is stronger when compared with positive control (Acarbose IC50 value of ‎45.3‎ µg/ml). The inhibition of glycosylation of hemoglobin of Terfezia claveryi methanol extract showed almost the same IC50 (33.1µg/ml) when compared the positive control, alpha-tocopherol (‎35.4µg/ml‎). In vivo antidiabetic study revealed that Terfezia claveryi methanol extract ‎ possessed good activity at a dose of 200 mg/kg through reducing the fasting plasma glucose level (122.1‎±‎3.0 mg/dl) when compared with positive control (Glibenclamide of ‎79.4±1.4‎ mg/dl) (p < 0.001). The results from this study indicated that Terfezia claveryi methanol extract exhibited considerable in vitro and in vivo antidiabetic activities. These possible activities could be useful to consider Terfezia claveryi ‎ as therapeutic antidiabetic candidate.

Download Full-text

Antineoplastic property of Ashwagandha for Paclitaxel concomitant, can induce p53-mediated apoptosis: In vitro search for anti-proliferative phytogent

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.1.0317 ◽

2020 ◽

Vol 13 (1) ◽

pp. 045-053

Author(s):

Gopinath Rana ◽

Rajesh Juneja

Keyword(s):

Western Blot ◽

Chemical Characterization ◽

In Vitro Assays ◽

Cancer Drug ◽

Hela Cell Lines ◽

Ic50 Value ◽

Proliferation And Apoptosis ◽

Ft Ir ◽

Vitro Effect

Ashwagandha (Withania somnifera L. Dunal) is an important and traditional medicinal herb found in India. It has been reported that, Ashwagandha has potential anti-proliferative as well as chemo-accelerative activity. The goal of this study was to investigate the most probable reason behind Ashwagandha’s anti-proliferative and chemo-accelerative activity. In addition, chromatographically isolate and chemically characterize some new compound which have antineoplastic property and find the mode of action of it. In vitro assays (MTT and Western blot) for anti-tumorigenic potentiality of the isolated drug were carried out on HT-29, KB and HeLa cell lines. In this experimental study, purification and chemical characterization (by UV, FT-IR, HPLC, LC-MS, 1H-NMR) of an anti-cancer drug has been found as Paclitaxel. MTT-assay shows an average IC50 value of the isolated Paclitaxel is 10 nM. Western blot data reveals there may be ROS-associated p53-MDM2-related cell proliferation and apoptosis by the drug’s in vitro effect.

Download Full-text

Kandungan Fitokimia dan Bioaktivitas Ekstrak Metanol Biji Palem Putri (Veitchia merillii)

Jurnal Kimia VALENSI ◽

10.15408/jkv.v2i1.3076 ◽

2016 ◽

Vol 2 (1) ◽

pp. 63-70

Author(s):

Adawiah Adawiah

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Radical Scavenging ◽

Positive Control ◽

Antidiabetic Activity ◽

Phytochemical Content ◽

Sample Extract ◽

Ic50 Value ◽

Ic50 Values ◽

Alpha Glucosidase

Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of 0.82 ppm, the level of toxicity is very toxic with LC50 values of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control. Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of 0.82 ppm, the level of toxicity is very toxic with LC50 values of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control. Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity DOI: http://dx.doi.org/10.15408/jkv.v2i1.3076

Download Full-text