Miswak starch-based gel as topical preparation for potential management of infective ulceration

Salvadora persica or Miswak is known for its antibacterial activities towards various bacteria species, including several oral pathogens. Although Miswak has many therapeutic potentials, the delivery medium is vital to ensure that the desired effects can be delivered to the target site. Ideally, starch is a biopolymer that exhibits muco-adhesive property, suitable as topical delivery material. Hence, this study was done to develop starch-based gel for Miswak antibacterial compound delivery system. In this study, Miswak hexane extract was incorporated into starch-based gels and stored at 4⁰C and room temperature. Miswak gel was tested for its antibacterial activity, and its stability was monitored by visual observation. As a result, the mean inhibitory zones of Miswak gels were found to be 22.67 ± 3.79 mm (4°C) and 20.67 ± 2.08 mm (room temperature) at day-1. The inhibitory zone decreased after 30 days with 13.00 ± 2.52 mm (4°C), and 12.00 ± 1.00 mm (room temperature). It was observed that the gels were stable where there were no colour changes, no phase separation and degradation, visualised up to day-60. In conclusion, the starch-based gel is a suitable delivery system for Miswak extract, thus as potential management for infective ulceration.

Download Full-text

One-pot Synthesis of Pyrazolone Sulfones by Iodine-catalyzed Sulfenylation of Pyrazolones with Aryl Sulfonyl Hydrazides Followed by Oxidation in Water

Current Organic Synthesis ◽

10.2174/1570179414666171020113745 ◽

2018 ◽

Vol 15 (3) ◽

pp. 380-387

Author(s):

Xia Zhao ◽

Xiaoyu Lu ◽

Lipeng Zhang ◽

Tianjiao Li ◽

Kui Lu

Keyword(s):

Double Bond ◽

Efficient Method ◽

Room Temperature ◽

Sulfonyl Chloride ◽

Antibacterial Activities ◽

Oxidation Reactions ◽

Reaction Conditions ◽

One Pot ◽

X Ray ◽

Amide Form

Aim and Objective: Pyrazolone sulfones have been reported to exhibit herbicidal and antibacterial activities. In spite of their good bioactivities, only a few methods have been developed to prepare pyrazolone sulfones. However, the substrate scope of these methods is limited. Moreover, the direct sulfonylation of pyrazolone by aryl sulfonyl chloride failed to give pyrazolone sulfones. Thus, developing a more efficient method to synthesize pyrazolone sulfones is very important. Materials and Method: Pyrazolone, aryl sulphonyl hydrazide, iodine, p-toluenesulphonic acid and water were mixed in a sealed tube, which was heated to 100°C for 12 hours. The mixture was cooled to 0°C and m-CPBA was added in batches. The mixture was allowed to stir for 30 min at room temperature. The crude product was purified by silica gel column chromatography to afford sulfuryl pyrazolone. Results: In all cases, the sulfenylation products were formed smoothly under the optimized reaction conditions, and were then oxidized to the corresponding sulfones in good yields by 3-chloroperoxybenzoic acid (m-CPBA) in water. Single crystal X-ray analysis of pyrazolone sulfone 4aa showed that the major tautomer of pyrazolone sulfones was the amide form instead of the enol form observed for pyrazolone thioethers. Moreover, the C=N double bond isomerized to form an α,β-unsaturated C=C double bond. Conclusion: An efficient method to synthesize pyrazolone thioethers by iodine-catalyzed sulfenylation of pyrazolones with aryl sulfonyl hydrazides in water was developed. Moreover, this method was employed to synthesize pyrazolone sulfones in one-pot by subsequent sulfenylation and oxidation reactions.

Download Full-text

Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Biomolecules ◽

10.3390/biom11020299 ◽

2021 ◽

Vol 11 (2) ◽

pp. 299

Author(s):

Reetika Singh ◽

Christophe Hano ◽

Gopal Nath ◽

Bechan Sharma

Keyword(s):

Silver Nanoparticles ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Room Temperature ◽

Xrd Analysis ◽

Antibacterial Activities ◽

Effect Of Temperature ◽

Human Pathogenic Bacteria ◽

Carissa Carandas ◽

Antioxidant And Antimicrobial Activity

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

Download Full-text

Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽

10.3390/antibiotics10080893 ◽

2021 ◽

Vol 10 (8) ◽

pp. 893

Author(s):

Olufunto T. Fanoro ◽

Sundararajan Parani ◽

Rodney Maluleke ◽

Thabang C. Lebepe ◽

Jose R. Varghese ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cell Viability ◽

Leaf Extract ◽

Room Temperature ◽

Klebsiella Oxytoca ◽

Cost Effective ◽

Average Diameter ◽

Antibacterial Activities ◽

Inhibition Concentration ◽

First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

Download Full-text

The Atmosphere of the Underground Electric Railways of London: A Study of its Bacterial Content in 1920

Journal of Hygiene ◽

10.1017/s0022172400008135 ◽

1923 ◽

Vol 22 (2) ◽

pp. 123-155 ◽

Cited By ~ 3

Author(s):

J. Graham Forbes

Keyword(s):

Body Temperature ◽

Room Temperature ◽

The Mean ◽

Bacterial Content ◽

The City

1. The bacterial content of the air of the Underground Railways, when the average of all results of the bacteriological investigations is taken, does not numerically compare unfavourably with the outside air of London.2. The ratio of the number of organisms growing at room temperature appears to be about 14 for railway air to 10 outside air. For those growing at body temperature the ratio is considerably higher, namely 2 to 1 respectively. The mean per litre, for room temperature organisms, is about 9 in railway air, 6·3 in the outside air; for body temperature organisms 4·6 for railway air, 2·2 for outside air.3. The bacterial content of platform air, except on the City and South London Railway, would appear to be higher than that of carriage air; the total mean for platform air being 52 and for carriage air 42·8 organisms per 5 litres, or a ratio of 16·4 and 13·5 respectively to 10 of the open air. The higher proportion in platform air is generally speaking to be accounted for by the greater amount of draught and dust disturbance.4. The ratios of the total bacterial content of railway carriage air and carriage and platform air on the six lines to open air are estimated in the following proportions:

Download Full-text

Lead inclusions in aluminium

MRS Proceedings ◽

10.1557/proc-157-247 ◽

1989 ◽

Vol 157 ◽

Cited By ~ 6

Author(s):

E. Johnson ◽

L. Gråbaek ◽

J. Bohr ◽

A. Johansen ◽

L. Sarholt-Kristensen ◽

...

Keyword(s):

Hysteresis Loop ◽

Room Temperature ◽

Noble Gas ◽

Melting Transition ◽

X Ray Diffraction ◽

Free Surfaces ◽

The Matrix ◽

Mean Size ◽

The Mean ◽

Spontaneous Phase

ABSTRACTIon implantation at room temperature of lead into aluminium leads to spontaneous phase separation and formation of lead precipitates growing topotactically with the matrix. Unlike the highly pressurised (∼ 1–5 GPa) solid inclusions formed after noble gas implantations, the pressure in the lead precipitates is found to be less than 0.12 GPa.Recently we have observed the intriguing result that the lead inclusions in aluminium exhibit both superheating and supercooling [1]. In this paper we review and elaborate on these results. Small implantation-induced lead precipitates embedded in an aluminium matrix were studied by X-ray diffraction. The (111) Bragg peak originating from the lead crystals was followed during several temperature cycles, from room temperature to 678 K. The melting temperature for bulk lead is 601 K. In the first heating cycle we found a superheating of the lead precipitates of 67 K before melting occurred. During subsequent cooling a supercooling of 21 K below the solidification point of bulk lead was observed. In the subsequent heating cycles this hysteresis at the melting transition was reproducible. The full width of the hysteresis loop slowly decreased to 62 K, while the mean size of the inclusions gradually increased from 14.5 nm to 27 nm. The phenomena of superheating and supercooling are thus most pronounced for the small crystallites. The persistence of the hysteresis loop over successive heating cycles demonstrate that its cause is intrinsic in nature, and it is believed that the superheating originates from the lack of free surfaces of the lead inclusions.

Download Full-text

The Coordination of Visual Observation and Instrumental Behavior in Early Infancy

Perception ◽

10.1068/p020307 ◽

1973 ◽

Vol 2 (3) ◽

pp. 307-314 ◽

Cited By ~ 54

Author(s):

Iize V Kalnins ◽

J S Bruner

Keyword(s):

Baseline Level ◽

Visual Observation ◽

Voluntary Control ◽

Early Infancy ◽

Initial Condition ◽

Instrumental Behavior ◽

Active Response ◽

The Mean ◽

Instrumental Control ◽

Clear Condition

Infants aged 5–12 weeks were shown a silent colour film whose clarity/focus was contingent on their sucking on a dummy nipple. In the ‘suck-for-clear’ condition the mean rate of sucking increased significantly over baseline level, and decreased when the contingency shifted to ‘suck-for-blur’. When the initial condition was suck-for-blur, sucking rate remained close to baseline level (even after the shift to suck-for-clear). Time spent looking at the clear film increased in both conditions, but there was little change in looking at the blurred pictures. With the introduction of the contingency conditions patterns of looking at the clearing and cleared pictures changed, and looking at the cleared picture increased in the suck-for-clear but not the suck-for-blur condition. Asymmetry of the results indicates that infants are better able to use an active response for instrumental means than to inhibit a response to achieve instrumental control. Implications for the development of voluntary control of action are explored.

Download Full-text

Comparison of Efficacy of Refrigerated Oxytocin with Oxytocin Kept at Room Temperature for the Prevention of Post-Partum Haemorrhage in Vaginal Deliveries in a Tertiary Care Setting

Open Access Journal of Gynecology ◽

10.23880/oajg-16000207 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Pandey U

Keyword(s):

Blood Loss ◽

Low Income ◽

Room Temperature ◽

Postpartum Haemorrhage ◽

Past History ◽

Post Partum ◽

Cold Chain ◽

Vaginal Deliveries ◽

History Of ◽

The Mean

Introduction: Postpartum haemorrhage is one of the leading causes of maternal death worldwide and it accounts for nearly one-quarter of all maternal deaths and almost half of all postpartum deaths in low-income countries. Primary postpartum haemorrhage (PPH) is the most common form of major obstetric haemorrhage. Materials and Methods: The study protocol was comprised of Consent, Measurement of Pre-delivery Hemoglobin, Administration of the Intervention, Measurement of postpartum blood loss and Measurement of Post-delivery (24-48 hours) Hemoglobin. Blood loss was measured using a calibrated drape. The drape was placed beneath the parturient buttocks and secured around her abdomen with ties. Blood loss was monitored for a minimum of one hour and was continued in the second hour in case of persistent bleeding. The drape with the collected blood was weighed on a scale. The weight of the drape and the container in which it is placed was deducted from the total recorded weight in order to obtain the weight of the blood collected in the drape. Blood loss weight in grams was converted to milliliters by dividing the figure in grams by 1.06 (blood density in grams per milliliter). Results: The cross tabulations were used to study the demographic, obstetrical and medical factors in women with obstetrical haemorrhage. Table 1 shows the selected sociodemographic characteristics of the study population. The mean age of cases and controls are 26.333.559 and 26.853.873 respectively. On comparison, they are statistically insignificant. (p=0.324). The educational, occupational and socioeconomic status was comparable between cases and controls (p >0.05). Table 2 shows Antenatal, intra-partum and post-partum data in cases and controls. Discussion & Conclusion: It is a study done in North India comparing the Oxytocin kept at room temperature with failure of maintenance of cold chain during transport and storage and the refrigerated Oxytocin. It is the common understanding and general training that Oxytocin must be stored in the refrigerator, failing which its efficacy reduces i.e. it, will not be effective in controlling PPH. During the study we compared the mean blood loss and change in hemoglobin levels in cases and control and despite the fact that major risk factor for PPH for example past history of PPH, past history of D&C, prolonged third stage labour duration, manual removal of placenta were comparable in both cases and controls, still the mean blood loss and change in hemoglobin values was more in cases than controls. This could be attributed to usage of market oxytocin which had failed cold chain maintenance resulted in less effective oxytocin in prevention of PPH, Hence causing more blood loss and drop in hemoglobin values. This shows the need of room temperature stable uterotonic drug in LMIC’s like ours. Recently room temperature stable carbetocin shows the potential as an effective uterotonic drug for the prevention of PPH. However according to various studies carbetocin cannot be used for induction or augmentation of labour so it cannot replace oxytocin fully, rather it acts as a part of collective PPH reduction strategy.

Download Full-text

Overcoming root dormancy and identifying the storage behaviour of Lilium polyphyllum seeds

Botany ◽

10.1139/cjb-2018-0161 ◽

2019 ◽

Vol 97 (2) ◽

pp. 161-166 ◽

Cited By ~ 1

Author(s):

Anurag Dhyani ◽

Carol C. Baskin ◽

Bhagwati Prasad Nautiyal ◽

Mohan Chandra Nautiyal

Keyword(s):

Room Temperature ◽

Seed Storage ◽

Seedling Production ◽

Spring Temperature ◽

Indole Butyric Acid ◽

Nitrogenous Compounds ◽

Morphophysiological Dormancy ◽

Mean Germination Time ◽

The Mean ◽

Endangered Medicinal Herb

Lilium polyphyllum D.Don ex Royle is a critically endangered medicinal herb of Himalaya. Seeds have epicotyl morphophysiological dormancy, and in the field a minimum of 34 weeks is required for root emergence. To date, treatments to decrease the time for root protrusion and seed storage behaviour have not been determined. We evaluated the effects of plant growth regulators and nitrogenous compounds as well as seed scarification on root emergence, and determined the storage behaviour of seeds. Root emergence at 20 °C was significantly increased by 100 ppm of indole butyric acid (IBA), KNO3, and thiourea, and by the application of NaOCl solution for 30 min. The mean germination time was significantly decreased by 50 ppm of gibberellic acid (GA3; 28 days) compared with 100 ppm GA3 (36 days). After 12 months of dry storage, the viability of seeds (TTC method) at room temperature (10–35 °C), 0–4 °C, and −20 ± 1 °C was 95%, 100%, and 100%, respectively; thus, the seeds have orthodox storage behaviour. Our recommendation for seedling production is to store the seeds at room temperature until they can be sown under warm wet conditions. Roots emerge after about 8 weeks, and then seeds should be given a 2-week cold moist treatment to break shoot dormancy. After 2 weeks of cold, transfer the seeds to warm (20 °C) spring temperature conditions to promote growth.

Download Full-text

Vancomycin Serum Protein Binding Determination by Ultrafiltration

Drug Intelligence & Clinical Pharmacy ◽

10.1177/106002808802200404 ◽

1988 ◽

Vol 22 (4) ◽

pp. 300-303 ◽

Cited By ~ 34

Author(s):

Bruce H. Ackerman ◽

E. Howard Taylor ◽

Keith M. Olsen ◽

Wedad Abdel-Malak ◽

Alexandras A. Pappas

Keyword(s):

Protein Binding ◽

Correlation Coefficient ◽

Pharmacokinetic Study ◽

Room Temperature ◽

Total Serum ◽

Serum Protein Binding ◽

Serum Concentrations ◽

Orthogonal Regression ◽

The Mean ◽

Serum Vancomycin

Sixty-two serum concentrations were obtained from 12 infected patients enrolled in a vancomycin pharmacokinetic study. Both unbound and total serum vancomycin concentrations were measured using ultrafiltration and a commercial fluorescent polarization immunoassay. Ultrafiltrates were obtained by centrifugation at 1000 × g for ten minutes at room temperature and their assay indicated a range in protein binding from 7.9 to 71 percent. The mean protein binding (mean ±SD) was 41.95 ± 14.15 percent. No measurable adsorption of vancomycin onto the ultrafiltration membrane was noted. Orthogonal regression of unbound versus total vancomycin concentrations was described by the equation y=0.597x-0.362 with a correlation coefficient of 0.948.

Download Full-text

SOLVOLYSABLE DEOXYCORTICOSTERONE CONJUGATES IN HUMAN URINE

Journal of Endocrinology ◽

10.1677/joe.0.0680273 ◽

1976 ◽

Vol 68 (2) ◽

pp. 273-281 ◽

Cited By ~ 2

Author(s):

C. L. COPE ◽

S. LOIZOU

Keyword(s):

Human Urine ◽

Room Temperature ◽

Urine Samples ◽

Variable Ratio ◽

The Mean ◽

Sulphate Conjugate ◽

Urine Specimens

SUMMARY The nature of the urinary conjugate converted by solvolysis, to free unconjugated deoxycorticosterone (DOC) was studied. A comparison of 11 solvolysis techniques has shown that the method employed in this study yielded 86% of the highest yield by any of the techniques tried. Three successive chromatographic systems on paper showed that no appreciable amounts of contaminants were present in the free DOC eluates, following solvolysis. By preparing authentic [3H]DOC sulphate and subjecting it to solvolysis it was shown that more than 90% of the tritiated DOC was recovered, after chromatography of the free DOC extract. This suggests that much of the solvolysable DOC in human urine is present in the form of the sulphate conjugate. The levels of DOC, excreted as the solvolysable conjugate in a variety of urine specimens, were shown to be much higher than those of free DOC, the former being 4·8 to 127 times higher than the amount of the latter. This highly variable ratio suggests that the site of production of solvolysable DOC is different from that for free DOC. The only correlation between free and solvolysable DOC was shown in dexamethasone-suppressed patients, in whom the mean percentage remaining after suppression was 30·6% for free DOC, 24·1% for solvolysable DOC and 22·2% for cortisol. As solvolysable DOC is present in much larger amounts in urine, care is necessary in the storage of urine samples in which free DOC estimates are to be made, as we found that urine specimens left at room temperature for 1 week could show rises of as much as 400% of their starting free DOC levels.

Download Full-text