scholarly journals Effect of Oral Supplementation for Rabbits of Pomegranate seed Extract on Some Serum Biochemical Parameters in Relation to oral Inflammation, oxidative stress, and wound healing

2019 ◽  
Vol 31 (2) ◽  
pp. 14-19
Author(s):  
Rezan M Omer ◽  
Dilyar A Baban ◽  
Bakhtiar M Ahmed
Keyword(s):  
Wound Healing ◽  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Serum Alkaline Phosphatase ◽  
Seed Extract ◽  
Total Proteins ◽  
Time Intervals ◽  
Oral Supplementation ◽  
Pomegranate Seed ◽  
Anti Inflammatory

Background: Pomegranate (punicagranatum L, Punicaceae), is an edible fruit consumed around the world. The edible part of pomegranate is rich in compounds that possess antioxidant and anti-inflammatory activities. The aim of this study is to investigate the antioxidant; anti-inflammatory and gingival wound healing effects of Punicagrantum L. seed extract oral supplementation in rabbit. Methods and Methods: Forty five male rabbits were divided into 3 groups, base line (5 rabbits) left without buccal gingival wound as( group 1),study group, 20 rabbits (group2) with buccal gingival wound treated with ethanolic extract of Punicagranatum L. seed extract and control, 20 rabbits (group 3) with buccal gingival wound only. Buccal gingival wounds were created on lower right central incisor and sutured removed after (7) days. Blood biopsies by cardiocentesis were collected at times (0, 3h, 1, 3 and 7days) for estimation of serum alkaline phosphatase activity, serum total proteins, and serum uric acid. Results: The results showed a significant increase in serum Alkaline phosphatase, total proteins and uric acid in all time intervals after buccal gingival wound, in rabbits receiving water, while their levels increased significantly only at time intervals of 3 hours and 1 day after gingival wound, in rabbits receiving pomegranate seed extraction. Conclusions: It has been concluded that oral Supplementation of pomegranate seed extract in rabbits can expedite the rate of healing of gingival wound

scholarly journals Serum Creatinine and Creatine Kinase as an Indicator for Gingival Wound Healing in Rabbits by Supplementation of Pomegranate Seed Extract

2021 ◽  
Vol 21 (2) ◽  
pp. 1-9
Author(s):  
Rezan Majeed Omer ◽  
Keyword(s):  
Wound Healing ◽  
Creatine Kinase ◽  
Serum Creatinine ◽  
Seed Extract ◽  
Control Group ◽  
Time Interval ◽  
Time Intervals ◽  
Baseline Group ◽  
Baseline Values ◽  
Pomegranate Seed

Background: Pomegranate (Punica granatum) is an edible fruit that has been described as a medical and therapeutic functional food in the Middle East and the Mediterranean. Objective: To investigate the use of serum creatinine (Cr) level and creatine kinase (CK) activity as indicators for gingival wound healing process rate in rabbits, supplemented on pomegranate seed extract (PSE). Patients and Methods: A total of 45 rabbit males were used. They were divided into 3 groups; 5 rabbits as a baseline group that left without a buccal gingival wound. 20 rabbits (5 rabbits per each time interval) as a study group with buccal gingival wound with PSE supplementation, and another 20 rabbits (5 rabbits per each time interval) as a control group with gingival wound without PSE supplementation. A buccal gingival wound was created on the lower right central incisor, and the suture was removed after (7) days. Blood samples were collected for the baseline group and at time intervals; 3 hour, 1, 3, 7 days after creating the wound for both control and study groups to determine serum Cr and serum CK. Results: Serum Cr and CK significantly increased in all time intervals after gingival wounds, in the control group compared with baseline values. Whereas the levels significantly increased in rabbits receiving PSE at intervals of 3 hours, 1 and 3 days after gingival wound, they returned to the baseline values, seven days after gingival wound incision. Conclusion: Serum Cr and CK increase in the buccal gingival wound, while oral supplementation of PSE can decline them to the baseline value after a period of time, therefore these parameters can be used as indicators for gingival wound healing rate. Keywords: Punicagranatum seed extract, gingival wound healing, creatinine, creatine kinase.

scholarly journals Serum biochemistry of 4-day-old ostriches (Struthio camelus)

2008 ◽  
Vol 28 (9) ◽  
pp. 423-426 ◽  
Author(s):  
Renata L. Miranda ◽  
Antonio V. Mundim ◽  
Álisson S. Costa ◽  
Ruyther V. Guimarães ◽  
Frederico O.C. Silva
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Biochemistry ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Total Proteins ◽  
Males And Females

An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds' sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, γ-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.

scholarly journals Serum uric acid, lipid profile and alkaline phosphatase levels in ischemic cerebrovascular accident patients

2021 ◽  
Vol 8 (8) ◽  
pp. 1171
Author(s):  
Gaurav Gupta ◽  
Saurabh Kishor ◽  
Aditya Kumar
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Ischemic Stroke ◽  
Serum Uric Acid ◽  
Cerebrovascular Accident ◽  
Serum Alkaline Phosphatase ◽  
Multiple Logistic Regression Analysis ◽  
Elevated Serum ◽  
Control Group ◽  
Significant Difference

Background: Stroke or cerebrovascular accident (CVA) is noted as the second cause of mortality, especially in the elderly population. Recent studies indicated that higher concentrations of uric acid are involved in various vascular diseases. The findings of previous investigations suggest that, elevated serum alkaline phosphatase (ALP) levels may have a pathophysiological character in the occurrence of atherosclerotic vascular disease (AVD) of the heart and brain. This study evaluated the association between serum uric acid (SUA) levels, serum lipid levels, serum alkaline phosphatase (ALP) levels, and changes in ischemic cerebrovascular accident patients.Methods: All patients with Ischemic cerebrovascular accident age >50 years were included based on their clinical, laboratory, and radiological findings (including computed tomography (CT)/magnetic resonance imaging (MRI)) those admitted in our hospital. As control group 200 healthy individuals matched for sex and age were recruited from the same demographic area.Result: Multiple logistic regression analysis findings proposed four components as significant predictors in ischemic cerebrovascular accident (serum uric acid, serum ALP, LDL and HDL. In this study, it was found, that patients with ischemic cerebrovascular accident had significant difference (p<0.001) in serum uric acid and serum ALP than normal patients (non-ischemic cerebrovascular accident patients).Conclusions: Patients with ischemic cerebrovascular accident had significant difference (p<0.001) in SUA and serum ALP than normal patients (non-ischemic cerebrovascular accident patients). High SUA levels were observed to be associated significantly with ischemic stroke. On the basis of our study design, we cannot clarify that the elevated levels are the risk of ischemic stroke and it requires further studies.

scholarly journals Evalution of Anti-Diabetic Potential of Aqueous Extract of “Luffa cylindrica” (Native Sponge/Sponge Gourd) Leaf and Seed on Alloxan Induced Diabetic Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
Luka, C. D. Abigail ◽  
E. A. Metuaghan
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Normal Control ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Serum Alkaline Phosphatase ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Luffa Cylindrica ◽  
Leaf Extracts

The study was carried out to evaluate the anti-diabetic effect of Luffa cylindrical (native sponge /sponge gourd) seed and leaf extracts in alloxan- induced diabetic rats. Sixteen experimental rats were divided into four groups of four rats each: a, diabetic control; b, normal control; c, diabetic rats treated with seed extract (400 mg/kg) and d, diabetic rats treated with leaf extract (400 mg/kg). The groups A, C and D rats were induced with diabetes intraperitoneally with alloxan (150 mg/kg bw). Phytochemical screening was carried out on the plant seed and leaf extracts and the following biochemical tests were carried out: blood glucose, serum lipid profile, serum alanine aminotransferase, serum aspartate aminotransferase, serum alkaline phosphatase, total protein, albumin, creatinine, urea, uric acid and some electrolytes like Na+, K+, HCO3-, and Cl- the administration of alloxan to experimental rats resulted in an increased level of most biochemical parameters; blood glucose, serum alanine aminotransferase, serum aspartate aminotransferase and serum alkaline phosphatase, serum total cholesterol, triglyceride, low density lipoprotein, creatinine, urea and uric acid. Luffa cylindrica seed and leaf extracts was administered to groups c and d diabetic rats respectively for two weeks, results were compared with normal control and diabetic control rats these parameters were found to be significantly (p<0.05) high in the diabetic groups than in the normal control groups. Treatment with the plant extract significantly (p<0.05) reduced elevated blood levels of glucose, cholesterol, triglyceride, alkaline phosphatase, amylase, aspartate aminotransferase, alanine aminotransferase, creatinine, urea, uric acid associated with alloxan-induced diabetic rats. The plant tested positive for alkaloids, flavonoids, saponins and tannins, negative for cardiac glycosides, phenols, resins, terpenes and steroids. Extracts of Luffa cylindrica seed and leaf has shown to have anti-diabetic and anti-lipidemic effects generally on alloxan induced diabetic rats. The study’s findings has shown that the plant possess hypoglycaemic and hypolipidaemic property and has supported the traditional use of Luffa cylindrica plant in the management of diabetes and its complications.

Role of Hypericum perforatum oil and pomegranate seed oil in wound healing: an in vitro study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ebru Uzunhisarcıklı ◽  
Mükerrem Betül Yerer
Keyword(s):  
Wound Healing ◽  
Hypericum Perforatum ◽  
Seed Oil ◽  
In Vitro Study ◽  
Seed Extract ◽  
Hacat Cells ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Better Than

Abstract This study aimed to investigate the wound-healing efficacy of Hypericum perforatum and pomegranate seed extract oil combined with curcumin as an anti inflammatory agent. A series of experiments were carried out to determine effective concentrations for H. perforatum oil, pomegranate seed extract oil, and curcumin. Ibuprofen was used as a positive control. The wound-healing effects of the applied compounds were tested according to the migration experiment model performed in HaCaT cells. A real-time cell analyzer (xCELLigence) was used to determine the cytotoxic/proliferative effects of H. perforatum, pomegranate seed oil, ibuprofen, and curcumin in HaCaT cells alone and their combined use at specified concentrations. After examining the noncytotoxic concentrations of H. perforatum oil, pomegranate seed oil, curcumin, and ibuprofen, migration experiments were performed to examine the wound healing properties. According to the results, the wound-healing efficacy of curcumin and H. perforatum combination was better than ibuprofen combinations. Also, according to the results, the wound-healing efficacy of curcumin and pomegranate seed oil combination was better than ibuprofen combinations. It was concluded that both oils had improved wound-healing properties in combination with curcumin or Ibuprofen.

Analysis of six serum components from rats infected with tetrathyridia of Mesocestoides corti

Parasitology ◽  
1985 ◽  
Vol 90 (3) ◽  
pp. 441-447 ◽  
Author(s):  
J. Chernin ◽  
A. Morinan
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
The Other ◽  
Total Proteins ◽  
Histochemical Analysis ◽  
Host Parasite Relationship ◽  
Parasite Relationship ◽  
Host Parasite ◽  
Serum Components

The serum from rats infected intraperitoneally (i.p.) and subcutaneously (s.c.) with the tetrathyridia of Mesocestoides corti was analysed for α-amylase, alkaline phosphatase, γ-glutamyltransferase, glucose, corticosterone and total proteins. A histochemical analysis for hepatic alkaline phosphatase was also carried out. A significant decrease in activity of serum alkaline phosphatase was detected in both the i.p. and s.c. infections, whereas a large increase in hepatic alkaline phosphatase was observed in i.p. infections. A significant increase in serum cortiscosterone was found in both the i.p. and s.c. infections. No changes were detected in the other four components. The relevance of these findings is discussed with respect to the host–parasite relationship.

Ultramicroscale Determination of Clinical Chemical Values for Blood during the First Four Days of Postnatal Life

1975 ◽  
Vol 21 (6) ◽  
pp. 746-750 ◽  
Author(s):  
Larissa deBaare ◽  
Jean Lewis ◽  
Helen Sing
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Total Protein ◽  
Inorganic Phosphorus ◽  
Postnatal Life ◽  
Total Proteins ◽  
Blood Constituents ◽  
Clinical Chemical ◽  
Term Newborns

Abstract Ultramicro procedures requiring 5-10 µl of serum or blood per analysis were used in determining blood constituents of healthy full-term newborns during the first four days of life. The resulting values appeared to be influenced by age, sex, and race. Values for total protein, albumin, urea nitrogen, and uric acid in serum decreased with time; serum inorganic phosphorus and whole-blood aldosaccharoses increased. Serum from females had higher values than that from males for total proteins, albumin, and inorganic phosphorus. The values for serum calcium and alkaline phosphatase were consistently higher in Negro than in white infants; values for uric acid were higher in the latter.

Comparative effects of Venlafaxine and Alendronate on Biochemical, Bone mechanical and Anti-inflammatory properties in ovariectomized rats

2021 ◽  
pp. 3553-3558
Author(s):  
Vadivelan Ramachandran ◽  
Punitha Nanjundan ◽  
Triveni Jasti ◽  
Manogaran Elumalai
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Inflammatory Cytokines ◽  
Bone Mineral ◽  
Serum Calcium ◽  
Serum Alkaline Phosphatase ◽  
Ovariectomized Rats ◽  
Bone Mineral Mass ◽  
Anti Inflammatory ◽  
Mineral Mass

The drug of choice in the treatment of postmenopausal osteoporosis is alendronate. Antidepressive agents are currently used in combination with alendronate to protect against depression and may affect the condition of osteoporosis. The aim is to study the comparative effects of venlafaxine and alendronate on biochemical, bone mechanical and anti-inflammatory properties in osteoporotic induced rats. 36 female Wistar albino rats were included (6 rats/group). Treated groups were ovariectomized bilaterally to induce osteoporosis. Rats were treated orally with alendronate (3mg/kg/day) and venlafaxine (20mg/kg/day) and combined alendronate and venlafaxine for 28 days. Body weight, serum alkaline phosphates, serum calcium, three point bending test, bone mineral mass and inflammatory cytokines The induction of osteoporosis showed significant elevated serum alkaline phosphatase, decreased serum calcium, body weight, bone mineral mass and inflammatory cytokines. Venlafaxine treatment did not ameliorate the changes in tested parameters, where at end of the experiment alendronate has significant improved with serum alkaline phosphatase, serum calcium, bone mineral mass, bone mineral density. The improvement was not affected by combining venlafaxine with alendronate whereas the venlafaxine treatment alone caused a significant deterioration of tested parameters. Venlafaxine is an anti-depressive agent that inhibits brain serotonin which leads to decrease in bone formation. Hence, from the above findings the combination of alendronate and venlafaxine showed worsen the condition of osteoporosis rats.

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