scholarly journals Serum Creatinine and Creatine Kinase as an Indicator for Gingival Wound Healing in Rabbits by Supplementation of Pomegranate Seed Extract

2021 ◽  
Vol 21 (2) ◽  
pp. 1-9
Author(s):  
Rezan Majeed Omer ◽  
Keyword(s):  
Wound Healing ◽  
Creatine Kinase ◽  
Serum Creatinine ◽  
Seed Extract ◽  
Control Group ◽  
Time Interval ◽  
Time Intervals ◽  
Baseline Group ◽  
Baseline Values ◽  
Pomegranate Seed

Background: Pomegranate (Punica granatum) is an edible fruit that has been described as a medical and therapeutic functional food in the Middle East and the Mediterranean. Objective: To investigate the use of serum creatinine (Cr) level and creatine kinase (CK) activity as indicators for gingival wound healing process rate in rabbits, supplemented on pomegranate seed extract (PSE). Patients and Methods: A total of 45 rabbit males were used. They were divided into 3 groups; 5 rabbits as a baseline group that left without a buccal gingival wound. 20 rabbits (5 rabbits per each time interval) as a study group with buccal gingival wound with PSE supplementation, and another 20 rabbits (5 rabbits per each time interval) as a control group with gingival wound without PSE supplementation. A buccal gingival wound was created on the lower right central incisor, and the suture was removed after (7) days. Blood samples were collected for the baseline group and at time intervals; 3 hour, 1, 3, 7 days after creating the wound for both control and study groups to determine serum Cr and serum CK. Results: Serum Cr and CK significantly increased in all time intervals after gingival wounds, in the control group compared with baseline values. Whereas the levels significantly increased in rabbits receiving PSE at intervals of 3 hours, 1 and 3 days after gingival wound, they returned to the baseline values, seven days after gingival wound incision. Conclusion: Serum Cr and CK increase in the buccal gingival wound, while oral supplementation of PSE can decline them to the baseline value after a period of time, therefore these parameters can be used as indicators for gingival wound healing rate. Keywords: Punicagranatum seed extract, gingival wound healing, creatinine, creatine kinase.

scholarly journals Effect of Oral Supplementation for Rabbits of Pomegranate seed Extract on Some Serum Biochemical Parameters in Relation to oral Inflammation, oxidative stress, and wound healing

2019 ◽  
Vol 31 (2) ◽  
pp. 14-19
Author(s):  
Rezan M Omer ◽  
Dilyar A Baban ◽  
Bakhtiar M Ahmed
Keyword(s):  
Wound Healing ◽  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Serum Alkaline Phosphatase ◽  
Seed Extract ◽  
Total Proteins ◽  
Time Intervals ◽  
Oral Supplementation ◽  
Pomegranate Seed ◽  
Anti Inflammatory

Background: Pomegranate (punicagranatum L, Punicaceae), is an edible fruit consumed around the world. The edible part of pomegranate is rich in compounds that possess antioxidant and anti-inflammatory activities. The aim of this study is to investigate the antioxidant; anti-inflammatory and gingival wound healing effects of Punicagrantum L. seed extract oral supplementation in rabbit. Methods and Methods: Forty five male rabbits were divided into 3 groups, base line (5 rabbits) left without buccal gingival wound as( group 1),study group, 20 rabbits (group2) with buccal gingival wound treated with ethanolic extract of Punicagranatum L. seed extract and control, 20 rabbits (group 3) with buccal gingival wound only. Buccal gingival wounds were created on lower right central incisor and sutured removed after (7) days. Blood biopsies by cardiocentesis were collected at times (0, 3h, 1, 3 and 7days) for estimation of serum alkaline phosphatase activity, serum total proteins, and serum uric acid. Results: The results showed a significant increase in serum Alkaline phosphatase, total proteins and uric acid in all time intervals after buccal gingival wound, in rabbits receiving water, while their levels increased significantly only at time intervals of 3 hours and 1 day after gingival wound, in rabbits receiving pomegranate seed extraction. Conclusions: It has been concluded that oral Supplementation of pomegranate seed extract in rabbits can expedite the rate of healing of gingival wound

220 Exploring the use of mesenchymal stem cells for treatment of mastitis and metritis in cattle

2020 ◽  
Vol 32 (2) ◽  
pp. 238
Author(s):  
R. Singh ◽  
S. Saini ◽  
S. Ansari ◽  
S. Jamwal ◽  
D. Malakar
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Real Time ◽  
Real Time Pcr ◽  
Local Group ◽  
Control Group ◽  
Time Intervals ◽  
And Control

The present study was carried out to isolate mesenchymal stem cells (MSCs) from adipose tissue of cattle (Bos indicus), characterise them, and apply them for the treatment of mastitis and metritis in the cow. Cattle MSCs were isolated from adipose tissue near the loin region of cow. Isolated adipose tissue was subjected to enzymatic digestion using 2% collagenase with agitation at regular intervals. The cells obtained after digestion were resuspended in cell culture flasks containing growth enriched medium and cultured under standard culture conditions. Alkaline phosphatase staining was used as one of the parameters to confirm cultured putative MSCs. Bovine Ad-MSCs were further characterised using real time-PCR by amplification of MSC-specific markers: CD73, CD90, and CD105 as positive markers and CD34, CD45, and CD79a as negative markers. Immunocytochemistry showed the presence of CD73, CD90, and CD105 on the cell surface. Three groups-control (C), local (L), and intravenous (IV)-with 6 cows suffering from mastitis were taken in each group and subjected to MSC transplantation through local and intravenous routes. Control group animals were subjected to antibiotic treatment only. Similarly, another three groups were taken with 6 cows in each group suffering from metritis. Post-transplantation wound healing, tissue repair, and reduction in inflammation were monitored for 26 days, at different time intervals; that is, after Days 1, 3, 7, and 15. Blood samples were also collected from animals at the same time intervals for real time-PCR. A similar examination was also done in metritis groups along with the analysis of the reduction in turbidity of cervical fluid at the abovementioned time intervals. Real time-PCR was performed to determine relative expression of genes for proliferative factors, anti-inflammatory cytokines, and antimicrobial peptides on cells isolated from blood collected at different time intervals. Gene expression in the local group of mastitis subjected to MSC injection was significantly higher than that of the IV and control group. The somatic cell count declined in both local and IV groups compared with the control group. Whereas the expression of the same genes in the IV group of metritis was significantly higher than that of the local and control groups of cows. The turbidity of cervical fluid and mucus was reduced in the IV group compared with the local group. In conclusion, we demonstrated the healing potential of MSCs in a cow model via MSC injection. Promising results were obtained in curing mastitis in both local and IV groups, whereas healing in the case of metritis was significantly higher in the IV group compared with both the control and local groups of cows. The study indicates the potential use of MSc for treatment of mastitis and metritis in cattle through wound healing and decreasing microbial infection.

Clinical observations on the course of oxytocinor prostaglandin E2/oxytocin-induced parturition in mares

2014 ◽  
Vol 17 (2) ◽  
pp. 347-351 ◽  
Author(s):  
M. Witkowski ◽  
K. Pawłowski
Keyword(s):  
Prostaglandin E2 ◽  
Positive Influence ◽  
Cervical Dilatation ◽  
Saline Control ◽  
Control Group ◽  
Time Interval ◽  
Time Intervals ◽  
Significance Level ◽  
Clinical Observations ◽  
Experimental Group

Abstract The objective of this study was to compare the course of parturitions induced with sole oxytocin with those induced with the combination of intracervical prostaglandin E2 jelly and oxytocin. For this purpose 13 mares in advanced pregnancy were allocated to the groups pretreated with either intracervical PGE2 (experimental group) or saline (control group) two hours before intravenous oxytocin (5 IU) administration. The mares were compared with respect to cervical dilation diameter (CDD) 20 min. after oxytocin injection. Time intervals from the first oxytocin dose to: the first external signs of parturition, the chorioallantois rupture, the delivery of a foal and time interval from the delivery of a foal to the placenta separation were measured. Cervical dilatation diameter as well as proportion of mares with cervical dilatation > 20 cm were significantly higher in the group of PGE2 treated mares comparing with control group (p = 0.0115 and p = 0.0490, respectively). All time intervals measured were statistically insignificant for both groups of mares, however time intervals from the first oxytocin dose to the first external signs of parturition, to the allantochorion rupture and to the delivery of a foal, were very close to the significance level (α = 0.05). To conclude, PGE/oxytocin combination has positive influence on the preparation of the uterine cervix to parturition. Moreover, it seems that PGE2 pretreatment reduced total oxytocin dose for successful parturition induction and shortened time elapsing between the first oxytocin dose and the delivery of a foal what is crucial for foal’s safety

scholarly journals An in vitro Study to evaluate the Effects of Various Polymerizing Conditions on the Flexural Strength of Autopolymerizing Resins

2017 ◽  
Vol 8 (1) ◽  
pp. 15-19
Author(s):  
Sanjeev Singh, ◽  
Abid Z Hussain, ◽  
RU Thombare, ◽  
BK Motwani, ◽  
Shweta Singh,
Keyword(s):  
Flexural Strength ◽  
Constant Pressure ◽  
Testing Machine ◽  
In Vitro Study ◽  
Bending Test ◽  
Control Group ◽  
Time Interval ◽  
Time Intervals ◽  
Increased Strength

ABSTRACT Aim To investigate the effect of variation in temperature and time intervals on the flexural strength of two brands of autopolymerizing resins polymerized at constant pressure. Materials and methods Two autopolymerizing resins RR Cold Cure resin (DPI) and RR-Rapid Repair Powder (Dentsply) were used. A total of 20 samples were fabricated for each group. Samples were polymerized at temperature ranges of 40°, 50°, 60°, and 70°C and at time intervals of 5, 10, and 15 minutes in the customized curing pot, maintaining pressure of 30 psi. Results Flexural strength was verified using universal testing machine using three-point bending test and was then compared with all samples and also with the samples cured in open air (control group). The samples cured at higher temperature, i.e., 60° and 70°C, have shown increased strength than those at 40° and 50°C as well as control group. Time interval of 10 minutes is considered sufficient to increase the strength of the resin, as per the results of this study. Conclusion The samples cured under varied condition of time and temperature but under constant pressure showed increased strength than those cured in open air. Clinical significance The effect of pressure and temperature has been demonstrated, which could be utilized for enhancing the durability of the prosthesis fabricated from autopolymerized resins. How to cite this article Singh S, Hussain AZ, Thombare RU, Motwani BK, Singh S, Mangalekar SB. An in vitro Study to evaluate the Effects of Various Polymerizing Conditions on the Flexural Strength of Autopolymerizing Resins. J Health Sci Res 2017;8(1):15-19.

scholarly journals An In Vitro Comparison of Quantitative Dissolution of Human Pulp in Different Irrigating Solutions

2010 ◽  
Vol 4 (2) ◽  
pp. 28-33
Author(s):  
Sonali Taneja ◽  
Rupali Chadha ◽  
Seema Dixit ◽  
Rohit Nayar
Keyword(s):  
Test Tube ◽  
Control Group ◽  
Time Interval ◽  
Equal Weight ◽  
Water Control ◽  
Pulp Tissue ◽  
Time Intervals ◽  
Filtration Method ◽  
Sample Test

ABSTRACT Pulp tissue from freshly extracted, intact vital premolars was removed in toto and was cut to get an approximate weight of 8.2 mg for each sample. Eighty samples thus obtained were divided into 4 groups of 20 samples each according to the irrigating solution used (5.25% NaOCl, 17% EDTA, BioPure MTAD and Distilled water (control group). Pulp tissue of specified equal weight (8.2 mg) was placed into each test tube of all groups carrying irrigants of measured volume (5ml each) at 37°C according to their specified subgroups time interval i.e. 10 min, 15 min, 20 min and 30 min respectively. The solution from each sample test tube was filtered and was left for overnight drying. The residual weight was calculated by filtration method. Results showed that the maximum amount of pulp was dissolved by 5.25% NaOCl at all time intervals. MTAD and 17% EDTA showed almost similar dissolution at all the time intervals.

Role of Hypericum perforatum oil and pomegranate seed oil in wound healing: an in vitro study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ebru Uzunhisarcıklı ◽  
Mükerrem Betül Yerer
Keyword(s):  
Wound Healing ◽  
Hypericum Perforatum ◽  
Seed Oil ◽  
In Vitro Study ◽  
Seed Extract ◽  
Hacat Cells ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Better Than

Abstract This study aimed to investigate the wound-healing efficacy of Hypericum perforatum and pomegranate seed extract oil combined with curcumin as an anti inflammatory agent. A series of experiments were carried out to determine effective concentrations for H. perforatum oil, pomegranate seed extract oil, and curcumin. Ibuprofen was used as a positive control. The wound-healing effects of the applied compounds were tested according to the migration experiment model performed in HaCaT cells. A real-time cell analyzer (xCELLigence) was used to determine the cytotoxic/proliferative effects of H. perforatum, pomegranate seed oil, ibuprofen, and curcumin in HaCaT cells alone and their combined use at specified concentrations. After examining the noncytotoxic concentrations of H. perforatum oil, pomegranate seed oil, curcumin, and ibuprofen, migration experiments were performed to examine the wound healing properties. According to the results, the wound-healing efficacy of curcumin and H. perforatum combination was better than ibuprofen combinations. Also, according to the results, the wound-healing efficacy of curcumin and pomegranate seed oil combination was better than ibuprofen combinations. It was concluded that both oils had improved wound-healing properties in combination with curcumin or Ibuprofen.

scholarly journals Rapid stain biomarker: A novel tool for forensic detection of semen (effect of time, different fabrics and vaginal secretions on its reliability)

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
A A Abdelrahman ◽  
S A Mahmoud ◽  
E A Attia
Keyword(s):  
Sexual Assault ◽  
Test Group ◽  
Personal Identification ◽  
The Other ◽  
Control Group ◽  
Time Interval ◽  
Genetic Profile ◽  
Human Semen ◽  
Time Intervals ◽  
Vaginal Secretions

Abstract Background The identification of semen stain is one of the most common human stains that can provide crucial information for crime scene reconstruction and forensic investigation. In sexual assault cases semen identification helps to prove victim’s allegations it also provides a material for DNA analysis that generate the genetic profile of the alleged suspect. The rapid Stain Identification of Human Semen (RSIDTM-Semen) bioassay is designed to detect specifically the presence of human semenogelin. Test development is completed within 10 minutes and can detect as little as 2.5 nL of human semen and it does not cross-react with other human or nonhuman tissues. The detection protocol can be completely integrated into the procedures for DNA extraction and analysis. Aim To assess the efficacy of RSIDTM – Semen strip test for the detection of human semen under some different variables (different fabrics, different time intervals and mixed with vaginal secretions) in order to be used in the routine forensic work of sexual assault cases and for further personal identification. Methodology: Semen samples were collected from four male participants. Vaginal specimens were collected from four female participants on cotton, linen or nylon-tipped swabs (2 swabs from each female). Each semen sample was divided into two portions; one used for semen only test group and the other mixed with vaginal secretions for the mixed test group. One of the fabrics tipped vaginal swab was mixed with semen for the mixed test group and the other used as a positive control group to test the sensitivity and specificity of the RSIDTM – Semen strip. The semen samples were deposited over different fabrics at the same time. All the samples were left to dry for 15 minutes at room temperature then extracted and analyzed according to the protocol designed for The Rapid Stain Identification Test (RSIDTM – Semen). The collected samples were studied as two test groups and one control group. Each of the previous groups, was categorized into 4 subgroups (a, b, c and d) according to the time interval of semen extraction (zero (on the spot), 2, 4, 6 and 10 days respectively). Results Semen could be identified in 100% of tested samples of the semen only group as well as of the combined semen and vagina group over cotton and linen fabrics at all the different tested time intervals. However, semen extracted from nylon fabric was identified in tested samples of the semen only group and of the combined semen and vagina group only at zero time only and couldn’t be identified at the rest of tested time intervals. Conclusion the current study evidenced that the new RSIDTM-semen kit is a reliable method for semen identification over different types of fabrics even in the presence of vaginal secretions. It also resists environmental factors up to 10 days.

scholarly journals The effect of Persea americana Mill. seed extract on inflammatory cells and fibroblast formation in tooth extraction socket healing

2021 ◽  
Vol 54 (4) ◽  
pp. 190
Author(s):  
Yessy Ariesanti ◽  
Irvan Septrian Syah Putra Rasad ◽  
Maylan Nimas ◽  
Nadira Syabilla
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Extraction Socket ◽  
Significant Difference ◽  
Socket Healing

Background: Inflammatory cells and fibroblasts have an essential role in the wound healing process. Persea americana Mill. seed categorises as a waste; it contains rich nutrients that can accelerate wound healing activity. Purpose: This study aims to determine the effect of Persea americana Mill. seed against inflammatory cells and fibroblast formation in tooth extraction socket healing. Methods: Ninety-six Sprague Dawley rats had their lower left molars removed. Forty-eight rats tested for inflammatory cells were divided into four groups: negative control group (IC1), positive control group (IC2), Persea americana Mill. seed extract concentrations of 50% (IE1) and 90% (IE2). Another 48 rats used for fibroblast were divided into three groups: the control group (FC1), Persea americana Mill. seed concentrations of 50% (FE1) and 90% (FE2). The gel was applied to the socket under general anaesthesia. Four rats from each group were decapitated for histopathological tissue preparations with Haematoxylin Eosin (HE) staining on the 3rd, 5th and 7th days for inflammatory cells and the 3rd, 5th, 7th and 14th days for fibroblast formation. The preparations for each research were scored under the microscope at 40x magnification. The obtained data was analysed using the Kruska—Wallis and the Mann—Whitney test. Results: A significant decrease (p<0.05) of inflammatory cells in IE2 on the 5th and 7th day. A significant increase (p<0.05) of fibroblast formation between treatment and control groups and no significant difference (p>0.05) between FE1 and FE2 was based on the interval days. Conclusion: Persea americana Mill. seed extract can decrease the inflammatory cells and accelerate the fibroblast formation in tooth extraction socket healing.

Judgments of Time Intervals by Young Children

1977 ◽  
Vol 45 (3) ◽  
pp. 715-720 ◽  
Author(s):  
Estelle R. Friedman
Keyword(s):  
Time Estimation ◽  
Control Group ◽  
Early Age ◽  
Time Interval ◽  
Time Intervals ◽  
Brief Training ◽  
Method Of Production ◽  
Conducting Research ◽  
The Right ◽  
Experimental Group

22 preschool children were either trained (experimental group) or not trained (control group) to indicate a 15-sec. time interval by the method of production. The accuracy was significantly improved by brief training. The children were generally accurate whether attending to the stopwatch at the right or left ear or given sound- or light-filled intervals. Neither age nor sex differences were found. These results demonstrate the possibility of conducting research on time-estimation at an early age if the procedures are relatively short and of interest to the young child. Because most of the children were unable to count as a guide to their judgments, the inner neural clock theory is supported.

DEVELOPMENT OF HERBOMINERAL FORMULATION FROM DAUCUS CAROTA (LINN) FOR WOUND HEALING EFFECTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (07) ◽  
pp. 66-68
Author(s):  
Rekha Tarasingh Rajput ◽  
Kashmira J. Gohil ◽  
Sanjeet Kumar ◽  
Keyword(s):  
Wound Healing ◽  
Breaking Strength ◽  
Positive Control ◽  
Control Group ◽  
Standard Group ◽  
Time Intervals ◽  
Healing Effect ◽  
Wound Model ◽  
Wound Healing Effect ◽  
Incision Wound

A herbomineral formulation was developed in ointment form by using herb extract, mineral and other excipients and evaluated for wound healing effect in excision and incision wound models in rats. Excision wounds sized 500 mm2 and 2 mm depth were used to study the rate of contraction of wound and epithelialization at different time intervals. Incision wounds were used for the study of tensile strength measured on the 10th day. It was observed that the herbomineral formulation in the excision wound model possessed a significant (p<0.001) wound healing effect compared to a positive control group and was nearly comparable with a standard group. In incision wounds, the formulation showed a significant increase in the skin breaking strength compared to control and reference standards.The wound healing effect of the formulation may be attributed to phytoconstituents like flavonoid and phenolic derivatives present in the root extract.The herbomineral ointment was also evaluated for various pharmaceutical parameters and was found to be stable for 45 days.

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