scholarly journals Study of etiology and antibiotic sensitivity of leading pathogens of postoperative infectious complications

2021 ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Infectious Complications ◽  
Chemotherapeutic Agents ◽  
Antimicrobial Drugs ◽  
Surgical Interventions ◽  
Opportunistic Bacteria ◽  
Microbiological Methods ◽  
Causative Agents

Summary. The problem of treatment and prevention of postoperative purulent complications in abdominal surgery in our time remains extremely urgent. This is due to an increase in the number of complex operations using modern technologies, an increase in the volume and duration of surgical interventions, as well as current antibiotic resistance problems. Objective: to study the structure of the leading causative agents of perioperative infectious complications in patients with surgical pathology and study their antibiotic sensitivity. Materials and methods - 576 patients, the average age of 45 ± 10.5 years, took part in the investigation, who were treated in the departments of surgery and intensive care unit of medical institutions of Vinnitsa region. A microbiological study of materials collected from patients was carried out (selection of secretions, fluid during drainage of superficial and deep surgical wounds). From the obtained biological material, a pure culture of the pathogen was isolated with its identification by morphological, tinctorial, cultural and biochemical properties according to generally accepted microbiological methods and using the Vitec automatic bacteriological analyzer (France). And a comparison was made of the profile of the sensitivity of the most significant pathogens to modern antibiotics. Results. According to the results of the study, it was revealed that the most common pathogens of surgical wound infections include opportunistic bacteria E.coli (22.0 %), S.aureus (19.0 %), S.epidermidis (13.0 %), E .faecalis (15 %), P.aeruginosa (10.0 %), A.baumanii (11.3 %). An analysis of the sensitivity to antibiotics of various classes of studied strains of the dominant causative agents of perioperative infectious complications showed that β-lactam antibiotics (meropenem (75–100 %), cefepime (65–100 %) and fluoroquinolones chemotherapeutic agents had the greatest antimicrobial activity. A slightly lower antimicrobial activity was found for third generation cephalosporins (70–85 %), amoxicillin-clavulanate (40–75%), aminoglycosides (tobramycin – 80 %, amikacin – 40 %). A high level of resistance of most grams of (-) microorganisms to ampicillin-sulbactam, cephalosporins of I–II generations in different ratios was determined. S.aureus resistance to azithromycin, clarithromycin is shown. Conclusions: the high prevalence of multiresistant isolates of pathogens (gram-positive (Staphylococcus aureus, enterococci), gram-negative pathogens (Escherichia, Klebsiella, enterobacteria, acinetobacteria, pseudomonads) emphasizes the need for dynamic microbiological monitoring in the surgical departments and the use of alternative antimicrobial drugs only.

scholarly journals ANALYSIS OF ANTIBIOTIC RESISTANCE OF K. PNEUMONIAE STRAINS ISOLATED IN A MULTIDISCIPLINARY HOSPITAL

2021 ◽  
Vol 19 (1) ◽  
pp. 31-35
Author(s):  
E. G. Antonova ◽  
◽  
I. V. Zhyltsou ◽  
Keyword(s):  
Antibiotic Sensitivity ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Antimicrobial Drugs ◽  
Resistance Level ◽  
Disk Diffusion Method ◽  
Actual Problem ◽  
Causative Agents ◽  
High Level

Background. The prevalence of infections caused by multiple resistant K. pneumoniae strains is an actual problem. Purpose. To investigate antibacterial resistance of hospital strains of K. pneumoniae – causative agents of purulent septic infections, to determine the resistance level of carbapenem-resistant strains of K. pneumoniae to polymyxins, to analyze their main profiles of antibiotic sensitivity. Material and methods. Antibiotic susceptibility of 146 clinical isolates of K. pneumoniae was determined using the disk diffusion method. The method of sequential microdilutions in broth with determination of MIC was used for isolates resistant to carbapenems. Results. For the majority of clinical isolates of K. pneumoniae (85.2%), only 3 antibiotics (colistin, tigecycline and amikacin) showed acceptable activity in vitro. For one strain resistance to all tested antimicrobial drugs was revealed. Conclusion. The data on extremely high resistance of K. pneumoniae to carbapenems, fluoroquinolones and aminoglycosides were confirmed. A high level of colistin resistance was also identified.

Sensitivity of vaginal and mammary bacterial microflora of sows from patients with postpartum dysgalactic syndrome to antibacterial drugs

2021 ◽  
pp. 66-71
Author(s):  
E. Latynina ◽  
G. Dyulger ◽  
A. Kremleva ◽  
Ю. Skomorina
Keyword(s):  
Antibiotic Sensitivity ◽  
Mammary Glands ◽  
Morphological Properties ◽  
Antibacterial Drugs ◽  
Gram Negative ◽  
Microbiological Methods ◽  
Causative Agents ◽  
Pure Cultures ◽  
Inflammatory Processes ◽  
Primary Identification

Purpose: Investigation of the sensitivity of conditionally pathogenic and pathogenic causative agents of postpartum disgalacting syndrome isolated from the pathological discharge of the vagina and the secretion of the mammary glands of patients with sorts to the most common antibacterial drugs.Materials and methods. The fence of the biological material was carried out within 2-3 days after supporting in sows of different ages and parity with the clinical manifestation of postpartum disgalacting syndrome from June to August 2021. In sows, the purse was taken in a mosper, milk, discharge from the vagina for the purpose of bacteriological research.Samples of vaginal wasches were sent in a special transportation environment of Ames. Samples of colostrum and milk (3-5 ml) were gained in sterile test tubes in compliance with the rules of antiseptics (the mammary glands were laid with warm water and 70% ethyl alcohol were treated). For the allocation and study of pure cultures of microorganisms from the above biomaterials produced crops on various nutrient media.The material brought to the laboratory was studied as follows: from the transport medium was carried out primary sowing on triptica-soybean agar, tryptichase-soybean broth, triptichase-soy agar with the addition of 5% of the defibrous blood of the ram; Samples were incubated in aerobic conditions at 37; The growth was taken into account after 24 hours. Then, pure cultures were isolated for the study of the cultural and morphological properties of the microorganisms obtained.The primary identification of the strains of microorganisms was carried out using the Microflex® LRF Bruker Maldi Biotyper system. The accuracy of the results obtained was confirmed by classical microbiological methods based on morphological, cultural and biochemical signs of microorganisms.The resistance of the isolated and identified pure crops to antibiotics was determined by diffusion in agar. In the study, the "advanced set of disks to estimate the antibiotic sensitivity of enterobacteria was used.Results. Inflammatory processes in the reproductive path of sows with postpartum diskalactic syndrome are caused by predominant gram-negative microorganisms, in lactic glands - associations of gram-positive and gram-negative microflora. The main conditionally pathogenic and pathogenic pathogens of endometritis and / or mastitis associated with postpartum diskalactic syndrome are the microorganisms of Escherichia, Enterobacter, Klebsiella, Actinobacillus, Rothia, Weisella, Pseudomonas, Proteus, Enterococcus, Streptococcus and Staphylococcus. It has been established that the overwhelming majority of microorganisms are sensitive to cephalosporine antibiotics: cefepim, zefisim, cefotaxim, ceftazidim, ceftriaxone, ceftinibuthen, cefuroxime.Conclusion. With respect to a large number of the most frequently used antibacterial drugs on this pig-breeding enterprise, high resistance is observed in microorganisms. Treatment of postpartum diskalactic syndrome sowers using antibacterial drugs is recommended to be carried out taking into account sensitivity to them allocated conditional and pathogenic microorganisms.

scholarly journals Antimicrobial activity of danofloxacin regarding to bacteria, pathogens of respiratory infections in pigs

2019 ◽  
Vol 21 (95) ◽  
pp. 22-26
Author(s):  
T. I. Stetsko ◽  
I. Ia. Kotsiumbas ◽  
Ya. M. Liubenko ◽  
V. N. Padovskyi ◽  
H. P. Uhryn
Keyword(s):  
Antimicrobial Activity ◽  
Respiratory Infection ◽  
Acute Respiratory Infection ◽  
Respiratory Infections ◽  
Streptococcus Suis ◽  
Chemotherapeutic Agents ◽  
Bordetella Bronchiseptica ◽  
Diffusion Method ◽  
Microbiological Methods ◽  
High Level

The objective of the research was to study the antimicrobial activity of danofloxacin, the third-generation fluoroquinolone antibiotic, against bacteria, pathogens of acute respiratory infection in pigs. Samples of nasal excretions were selected from clinically sick weaned piglets with acute respiratory infection for microbiological investigation. The sensitivity test, carried out by the disc-diffusion method, showed a high level of the microflora sensitivity of the inflammatory exudate to danofloxacin. Bacteria Streptococcus suis and Bordetella bronchiseptica have been isolated and identified from biomaterial by generally accepted microbiological methods. The degree of antimicrobial activity of danofloxacin against isolated strains of microorganisms was established by determination the minimum inhibitory concentration (MIC) of danofloxacin for isolated bacteria by consecutive dilutions in a liquid nutrient medium. The MIC average of danofloxacin for Streptococcus suis isolates (n = 20) was 0.33 ± 0.082 μg/ml and for Bordetella bronchiseptica isolates (n = 8) – 0.21 ± 0.044 μg/ml. The obtained results showed a high level of bacteriostatic activity of danofloxacin regarding bacterial isolates, pathogens of acute respiratory infection in pigs. Danofloxacin, like other fluoroquinolones, is a critical antimicrobial substance for veterinary medicine. Therefore, chemotherapeutic agents based on this antibiotic can serve as a drug of choice for empirical treatment of pigs with acute respiratory infections of bacterial etiology. To right choose an effective agent for etiotropic therapy and minimize the selection of resistant strains of microorganisms, the antimicrobial susceptibility of isolated bacteria should be pre-established.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

Analysis of the monitoring study of the antibiotic-resistance of the agents of purulent-inflammatory processes of soft tissue

2018 ◽  
Vol 22 (2) ◽  
pp. 285-288
Author(s):  
A.P. Prevar ◽  
A.V. Kryzshanovskaya ◽  
V.A. Radionov ◽  
V.M. Mrug
Keyword(s):  
Soft Tissues ◽  
High Sensitivity ◽  
Biochemical Properties ◽  
Antimicrobial Drugs ◽  
Strict Adherence ◽  
E Coli ◽  
Monitoring Study ◽  
Treatment Measures ◽  
Inflammatory Processes ◽  
Main Factor

The main factor in the treatment of suppurative and inflammatory processes is the timely optimization of treatment measures taking into account the nature of the microflora and its susceptibility to antimicrobial drugs. The purpose of the study is to monitor the spectrum of microorganisms – pathogens of purulent-inflammatory processes of soft tissues in surgical patients; study of the sensitivity of isolated strains to antibiotics. The material was collected in accordance with aseptic rules. The identification of a pure culture of bacteria was carried out according to morphological, culture, biochemical properties, and the presence of virulence enzymes. Sensitivity of bacteria to antibiotics was determined by the standard disks method (by Kirby-Bauer’s). 255 patients with purulent-inflammatory processes of soft tissues were examined for the period from 2014 to 2017. 229 strains of isolated bacteria were included to Escherichia coli, Citrobacer freundii, Enterobacter cloacae, E.aerogenes, Proteus vulgaris, Staphylococcus aureus, S.epidermidis, Streptococcus pyogenes, S.viridians, S.agalactiae, Pseudomonas aeruginosa. The main cause of purulent-inflammatory processes of soft tissues is Staphylococci (67,2%). Compared to previous studies, the number of P.aeruginosa isolated cultures increased (7.9%). In monoculture and in association with other microorganisms, E. coli (9.6% of cases), E.cloacae et aerogenes (3.9% of cases), P.vulgaris (3.9% of cases), C.freundi (2.5% of cases), S.agalactiae, S.pyogenes, S.viridans (3.5%). The number of associated sows reaches 12%. Clinical strains of microorganisms remain most sensitive to fluoroquinolones, cephalosporins, and also retains high sensitivity to gentamicin, lincomycin, rifampicin, which is important for empirical antibiotic therapy. To increase the effectiveness of antibacterial therapy, strict adherence to the mode of appointment of antibiotics, justification of indications, a combination of antibiotics of different spectrum of action, mandatory correction after determining the sensitivity of the pathogen.

scholarly journals Detection of Nocardia by 16S Ribosomal RNA Gene PCR and Metagenomic Next-Generation Sequencing (mNGS)

2022 ◽  
Vol 11 ◽  
Author(s):  
Juanjuan Ding ◽  
Bing Ma ◽  
Xupeng Wei ◽  
Ying Li
Keyword(s):  
16S Rrna ◽  
Ribosomal Rna ◽  
Human Herpesvirus ◽  
Antibiotic Sensitivity ◽  
Common Species ◽  
16S Ribosomal Rna ◽  
Clinical Samples ◽  
Klebsiella Pneumonia ◽  
Antimicrobial Drugs ◽  
Detection And Identification

In this study, the aim was to investigate the discriminatory power of molecular diagnostics based on mNGS and traditional 16S ribosomal RNA PCR among Nocardia species. A total of fourteen clinical isolates from patients with positive Nocardia cultures and clinical evidence were included between January 2017 and June 2020 in HeNan Provincial People’s Hospital. DNA extraction and 16S rRNA PCR were performed on positive cultures, and pathogens were detected by mNGS in these same samples directly. Among the 14 Nocardia isolates, four species were identified, and N. cyriacigeorgica (8 cases) is the most common species. Twelve of the 14 Nocardia spp. isolates were identified by the two methods, while two strains of N. cyriacigeorgica were not identified by mNGS. All tested isolates showed susceptibility to trimethoprim-sulfamethoxazole (SXT), amikacin and linezolid. Apart from Nocardia species, other pathogens such as Acinetobacter baumannii, Klebsiella pneumonia, Aspergillus, Enterococcus faecalis, Human herpesvirus, etc., were detected from the same clinical samples by mNGS. However, these different pathogens were considered as colonization or contamination. We found that it is essential to accurately identify species for determining antibiotic sensitivity and, consequently, choosing antibiotic treatment. 16S rRNA PCR was useful for identification of nocardial infection among species, while this technique needs the clinicians to make the pre-considerations of nocardiosis. However, mNGS may be a putative tool for rapid and accurate detection and identification of Nocardia, beneficial for applications of antimicrobial drugs and timely adjustments of medication.

scholarly journals Investigation of the antimicrobial activity of danofoloxacin against bacteria pathogens in goats

2020 ◽  
Vol 22 (97) ◽  
pp. 74-78
Author(s):  
T. I. Stetsko ◽  
Ya. M. Liubenko ◽  
V. N. Padovskyi ◽  
L. L. Ostrovska ◽  
O. Yo. Kalinina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
Chemotherapeutic Agents ◽  
Diffusion Method ◽  
Bacteriostatic Activity ◽  
Intestinal Infection ◽  
Sensitivity Testing ◽  
First Choice

Fluoroquinolones are critical antimicrobials for both human and veterinary medicine. Due to their unique mechanism of antimicrobial action and good pharmacokinetic properties, they are often the first choice drugs in the treatment of bacterial infections in animals. The purpose of the investigation was to study the antimicrobial activity of a third-generation fluoroquinolone antibiotic of danofloxacin against bacteria, pathogens of respiratory and intestinal infection in goats. The samples of the nasal outflows (respiratory infection) and fecal masses (intestinal infection) were collected from clinically ill goats for microbiological studies. The sensitivity test of the microflora of the biomaterial, carried out by the disco-diffusion method, showed that the microorganisms of all the samples were sensitive to danofloxacin. Bacteria Streptococcus pneumonia (n = 10), Staphylococcus aureus (n = 4) and Escherichia coli (n = 2) were isolated and identified from nasal exudate samples (n = 10). Pathogenic strains of Escherichia coli were isolated from all faecal samples (n = 12). The degree of bacteriostatic activity of danofloxacin was determined by establishing its minimum inhibitory concentration (MIC) for bacterial isolates by sequential dilutions in a liquid nutrient medium. The average MIC of danofloxacin for Streptococcus pneumoniae isolates was 0.26 ± 0.13 μg/ml and for Staphylococcus aureus isolates – 0.25 ± 0.075 μg/ml. For Escherichia coli strains isolated from faeces of goats suffering from coli infection, the average MIC of danofloxacin was 0.38 ± 0.12 μg/ml (range 0.2 to 0.8 μg/ml). Antimicrobial sensitivity testing have shown a high level of bacteriostatic activity of danofloxacin against bacteria, pathogens of respiratory and intestinal infections in goats. This may be the argument for the use of danofloxacin-based chemotherapeutic agents in the treatment of bacterial infections in goats, especially for the empirical approach to therapy.

scholarly journals A new group of compounds derived from 4-, 5-, 6- and 7-aminoindoles with antimicrobial activity

2018 ◽  
Vol 4 (3) ◽  
pp. 27-36 ◽  
Author(s):  
Irina Stepanenko ◽  
Semen Yamashkin ◽  
Yuliya Kostina ◽  
Alyona Batarsheva ◽  
Mikhail Mironov
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Biologically Active ◽  
Diffusion Method ◽  
Significant Activity ◽  
Clinical Strains ◽  
Causative Agents ◽  
New Compounds

Introduction. The problem of antibiotic resistance of microorganisms is becoming more urgent in the twenty-first century. Microorganisms possess an evolutionary adaptive capacity. Non-adherence to the basic principles of rational antibiotic therapy leads to menacing consequences. More and more pathogenic microbes are becoming resistant to two or more antibiotics. The search for new compounds with antimicrobial activity is one of the principles for overcoming the antibiotic resistance of microorganisms. Materials and methods. Eighteen test-strains of microorganisms and more than 2000 clinical strains of microorganisms, representating the families Micrococcaceae, Streptococcaceae, Enterobacteriaceae, Moraxellaceae, Pseudomonadaceae, Sphingomonadaceae, Xanthomonadaceae were studied for sensitivity to the compounds derived from 4-, 5-, 6- and 7-aminoindoles. A method of serial dilutions to determine the minimal inhibitory concentration (MIC) of the compounds under study was used in the study, as well as a disc diffusion method. Results and discussion. Sensitivity of the test-strains and of clinical strains of microorganisms to the resulting compounds was studied. The compounds based on substituted 4-, 5-, 6-, 7-aminoindoles showed different activity against the test strains and experimental strains of microorganisms in vitro. It was found that the marked antibacterial activity was exhibited by the compounds containing a trifluoromethyl group. The most significant activity was noted in amides and pyrroloquinolones based on 4-aminoindole, 6-aminoindole and 7-aminoindole.The most effective compounds with laboratory codes 5D, 7D, 39D, S3, HD, 4D showed a pronounced antibacterial activity. Conclusion. Antimicrobial activity of the substituted amides and pyrroloquinolines on the basis of 4-, 5-, 6-, 7-aminoindoles was etermined in our study, as well as the spectra of their action against Gram-positive and Gram-negative microorganisms, which are causative agents of non-specific and certain specific human infectious diseases. Moreover, we evaluated the synthetic potentials of the substituted 4-, 5-, 6-, 7-aminoindoles as the starting compounds for synthesizing a series of indolylamides and pyrroloquinolines. Also, the prospects for targeted synthesis of biologically active compounds based on indole-type aromatic amines were determined.

scholarly journals Antibiotic prophylaxis of acute postoperative endophthalmitis

2021 ◽  
Vol 21 (4) ◽  
pp. 220-226
Author(s):  
V.P. Nikolaenko ◽  
◽  
D.F. Belov ◽  
◽  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Povidone Iodine ◽  
Legal Aspects ◽  
Infectious Complications ◽  
Published Data ◽  
Antibacterial Prophylaxis ◽  
Postoperative Endophthalmitis ◽  
Bacterial Strains ◽  
Routes Of Administration ◽  
Causative Agents

This paper reviews current aspects of antibacterial prophylaxis of acute endophthalmitis, a dangerous complication of cataract surgery. The lack of a common standard for preventing phaco infectious complications, a growing number of resistant bacterial strains, and legal aspects of the lack of some antibiotic formulations and routes of their administration with established efficacy in Russia account for the importance of this issue. The authors provide a list of primary causative agents of endophthalmitis and specify their antimicrobial susceptibility, highlight the importance of using antiseptics to prevent endophthalmitis, and describe in detail routes of administration of antibiotics, including conventional ones (subconjunctival injections, topical use) and whose actively promoted (intracameral, transzonular). In conclusion, the authors suggested a rational antimicrobial preventive algorithm for endophthalmitis after phaco based on the analysis of recent Russian and foreign published data and adapted to the Russian health care system. Keywords: phacoemulsification, endophthalmitis, antiseptic, antibiotic, prophylaxis, antibacterial resistance, fluoroquinolones, aminoglycosides, povidone-iodine, intracameral administration. For citation: Nikolaenko V.P., Belov D.F. Antibiotic prophylaxis of acute postoperative endophthalmitis. Russian Journal of Clinical Ophthalmology. 2021;21(4):220–226 (in Russ.). DOI: 10.32364/2311-7729-2021-21-4-220-226.

Ampicillin-augmented silver nanoparticles for synergistic antimicrobial response: A promising therapeutic approach

2021 ◽  
Vol 22 ◽  
Author(s):  
Kashan Khan ◽  
Mohd Aamir Qureshi ◽  
Ameer Azam ◽  
Moinuddin ◽  
Javed Musarrat ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Bacterial Infections ◽  
Colloidal Stability ◽  
Resistant Bacteria ◽  
Antimicrobial Drugs ◽  
Vis Spectroscopy ◽  
Microbial Infections ◽  
Ft Ir

Aims: Globally Scientists are working to find more efficient antimicrobial drugs to treat microbial infections and kill drug-resistant bacteria. Background: Despite the availability of numerous antimicrobial drugs bacterial infections still poses a serious threat to global health. Due to a constant decline in the effectiveness of antibiotics owing to their repeated exposure as well as shortlasting antimicrobial activity, led to the demand for developing novel therapeutic agents capable of controlling microbial infections. Objective: In this study, we report antimicrobial activity of chemically synthesized silver nanoparticles (cAgNPs) augmented with ampicillin (amp) in order to increase antimicrobial response against Escherichia coli (gram –ve), Staphylococcus aureus (gram +ve) and Streptococcus mutans (gram +ve). Methods: Nanostructure, colloidal stability, morphology and size of cAgNPs before and after functionalization were explored by UV-vis spectroscopy, FT-IR, zeta potential and TEM. The formation and functionalization of cAgNPs was confirmed from UV-vis spectroscopy and FT-IR patterns. From TEM the average sizes of cAgNPs and cAgNP-amp were found to be 13 and 7.8 nm respectively, and change in colloidal stability after augmentation was confirmed from zeta potential values. The antimicrobial efficacies of cAgNP-amp and cAgNPs against E. coli S. aureus and S. mutans were studied by determining minimum inhibitory concentrations (MICs), zone of inhibition, assessment of viable and non-viable bacterial cells and quantitative assessment of biofilm. Results & Discussion: Our results revealed cAgNP-amp to be highly bactericidal compared to cAgNPs or amp alone. The nano-toxicity studies indicated cAgNP-amp to be less toxic compared to cAgNPs alone. Results: This study manifested that cAgNPs show synergistic antimicrobial effect when they get functionalized with amp suggesting their application in curing long-term bacterial infections.

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