scholarly journals Pemanfaatan Ekstrak Daun Kemangi (Ocimum sanctum) sebagai Repelan Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (3) ◽  
pp. 329
Author(s):  
Linda Barus ◽  
Agus Sutopo
Keyword(s):  
Musca Domestica ◽  
Disease Transmission ◽  
Leaf Extract ◽  
Observation Time ◽  
Ocimum Sanctum ◽  
Test Results ◽  
Transmission Control ◽  
Anova Analysis ◽  
House Flies ◽  
Two Factors

<p><span>House flies are the species that play the most role in public health problems, namely as a vector of disease transmission. Control of house flies is needed without polluting the environment. The alternative developed is the use of plants as vegetable pesticides. Basil leaves used as basil leaf extract are used as vegetable insecticides to control house flies <em>(Musca domestica).</em> This research is an experiment to see the ability of basil leaf extract as a bioinsecticide on the repulsion/repellency of house flies. This research is experimental and analyzed using a two-way ANOVA analysis. The study consisted of two factors, the first factor was the dose of basil leaf extract: 0%, 20%, 30%, dan 40%, and the second factor, the time of observation of the repelling power of house flies/repelan for 5 minutes, 10 minutes and 15 minutes. Statistical test results stated that basil leaf extract: dose 0% sig value 0,011&lt;0,05, dose 20% sig value 0,178&gt;0,05, dose 30% sig value 0,031&lt;0,05 and dose 40% sig value 0,007&lt;0,05, so that the dose of 0% has an effect for the observation time of 15 minutes, the dose of 20% does not affect the repelling/repelling power of house flies, for observations of 5, 10 and 15 minutes, the dose of 30% and the dose of 40% affects, for all time observations. </span></p>

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

scholarly journals PENGARUH PESTISIDA NABATI TERHADAP KEMATIAN LALAT RUMAH (MUSCA DOMESTICA)

2019 ◽  
Vol 13 (1) ◽  
pp. 11
Author(s):  
Novie Elvinawaty Mauliku
Keyword(s):  
Musca Domestica ◽  
Statistical Test ◽  
Lemon Juice ◽  
Effective Concentration ◽  
P Value ◽  
Test Results ◽  
House Flies ◽  
Post Hoc Analysis ◽  
Post Hoc ◽  
True Experiment

House flies (Musca domestica) are a vector that caused diary so it needs to be controlled by using plant-based insecticide such as Lime (Citrus aurantifolia). Lime had active contains such as saponins, tannins, flavonoids and essential oils. This study was to impact of lemon juice to the death of house flies (Musca domestica). This study design is the True Experiment. The lime was defined  with three concentrations there are 25%, 27% and 29% of the death of house flies (Musca domestica) which is given three repetitions to 300 samples. Data was analyzed using univariate, bivariate used Kruskal Wallis and post hoc analysis used bonferonni tests to determine the effectiveness of concentration. The results found out that the percentage of dead houseflies (Musca Domestica) at a concentration of 25% on average by 53%, the concentration of 27% on average by 71% and 29% concentrations by an average of 92%. Statistical test results obtained that there are differences in various concentrations of lime juice to the death of house flies (Musca Domestica) with a p value (0.014). The  effective concentration was at a 29% concentration against houseflies death 92%..

scholarly journals Efektivitas campuran ekstrak daun kari (Murraya koeginii) dengan daun sirsak (Annona murricarta linn) sebagai insektisida dalam menghentikan larut rumah (Musca domestica)

2021 ◽  
Vol 2 (2) ◽  
pp. 142
Author(s):  
Syahrizal Syahrizal ◽  
Junaidi Junaidi
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Human Life ◽  
P Value ◽  
Annona Muricata ◽  
House Flies ◽  
Curry Leaves ◽  
Significant Difference ◽  
The One ◽  
One Way Anova

Background: Flies (Musca domestica) can act as vectors of typhus, dysentery, and cholera. Various methods have been used to control flies from around human life, either mechanically (light traps), a chemical in the form of toxic baits or insecticides, or with scents that are repellent. A mixture of curry leaf extract with soursop leaves can be used as an insecticide to repel house flies. Because curry leaves and soursop leaves contain compounds, tannins, saponins, and flavonoids that flies do not like.Objective: The purpose of this study was to determine the effectiveness of a mixture of curry leaves with soursop leaves as a natural insecticide in repelling the flies (Musca Domestica).Methods: This research is an experimental design with a mixture of curry leaf extract with soursop leaves on the power of repelling house flies. This research was conducted in 2019 in Darul Kamal District, Aceh Besar. The object of the research was 450 house flies, which consisted of five treatments with three repetitions. Data analysis was carried out with the one-way ANOVA statistical test.Results: The descriptive results showed that at a dose of 10gr/100ml the average flies were able to repel 37 flies, at a dose of 20 gr/100ml were 44, at a dose of 30 gr/100ml were 59 and at a dose of 40 gr/100ml was 70. Of the four treatments, the most effective dose was at a dose of 40gr/100ml, which was 70 (77.7%). Statistically, it showed that there was a significant difference (p < 0.05) between various doses/treatments of mixed extracts of curry leaves (Murraya koeginii) and soursop leaves (Annona muricata Linn) on the repelling power of house flies (Musca domestica), with p-value = 0.000.Conclusion: A mixture of curry leaf extract (Murraya koeginii) with soursop leaf (Annona muricata Linn) is effective as an insecticide in repelling house flies (Musca domestica).

scholarly journals Potensi Ekstrak Daun Kemangi (Ocimum basilicum) Sebagai Repellent Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (1) ◽  
pp. 55
Author(s):  
Gracia Satyawestri Pribadi ◽  
Marlik Marlik
Keyword(s):  
Public Health ◽  
Developing Countries ◽  
Musca Domestica ◽  
Leaf Extract ◽  
Ocimum Basilicum ◽  
Control Group ◽  
Control Group Design ◽  
House Flies ◽  
Group Design ◽  
Post Test

Diarrhea is still a problem for public health in developing countries like Indonesia, with house flies (Musca domestica) as vectors. One way to control flies that are safe and natural is to use plant repellents including basil leaves. This study aimed to analyze the potential of basil leaf extract as a repellent, using a post test only with control group design, with 25 house flies (Musca domestica) as objects with 6 replications. Basil leaf extract was mixed into wax with a concentration of 0% (control), 17.5%, 20%, and 22.5% which was exposed to house flies within 1 hour. The results showed that basil leaf extract can be used as a repellent for house flies. Based on the percentage of aromatic waxing power, it was known that the higher the concentration of basil leaf extract, the higher the repelling power produced. Keywords: basil leaves, repellent, house flies ABSTRAK Diare masih menjadi masalah bagi kesehatan masyarakat di negara berkembang seperti di Indonesia, dengan lalat rumah (Musca domestica) sebagai vektor. Salah satu cara pengendalian lalat yang aman dan alami adalah menggunakan repellent tumbuhan antara lain daun kemangi. Penelitian ini bertujuan untuk menganalisis potensi ekstrak daun kemangi sebagai repellent, menggunakan rancangan post test only with control group, dengan lalat rumah (Musca domestica) sejumlah 25 ekor sebagai obyek dengan 6 kali replikasi. Ekstrak daun kemangi dicampurkan ke dalam lilin dengan konsentrasi 0% (kontrol), 17.5%, 20%, dan 22.5% yang dipaparkan pada lalat rumah dalam waktu 1 jam. Hasil penelitian menunjukkan bahwa ekstrak daun kemangi dapat digunakan sebagai repellent untuk lalat rumah. Berdasarkan persentase daya tolak lilin aromatik, diketahui bahwa semakin tinggi konsentrasi ekstrak daun kemangi, maka akan semakin tinggi daya tolak yang dihasilkan. Kata kunci: daun kemangi, repellent, lalat rumah

scholarly journals Antifungal activity of leaf extract against mycotoxin producing fungi

2020 ◽  
Vol 11 (2) ◽  
pp. 2650-2656
Author(s):  
Varsha A Bhalerao ◽  
Ashok M Chavan
Keyword(s):  
Antifungal Activity ◽  
Azadirachta Indica ◽  
Disease Transmission ◽  
Leaf Extract ◽  
Economic Loss ◽  
Storage Condition ◽  
Aspergillus Ochraceus ◽  
Ocimum Sanctum ◽  
Calotropis Procera ◽  
Datura Metel

About 90% of the world food crops are being produced by using seeds. Healthy seeds are important for production of healthy crop and these seeds are also responsible for disease transmission. It happens either in field or in post-harvest storage condition. Due to these seed borne fungi, seed get deteriorated which may cause a great economic loss as well as several types of abnormalities occur in seeds. Major storage grain fungi contaminated with mycotoxin, mycotoxins contaminants of fungal origin occurring worldwide and characterized by its acute and chronic toxic effects on human health. In the present attempt were made to study the antifungal activity of leaf extract against mycotoxin producing fungi. Fungi isolated from stored seed grains like  cereals, pulses, infected oil seeds were collected from from Marathwada region of Maharashtra, India. Antifungal activity of leaf extract of Calotropis procera, Azadirachta indica, Ocimum sanctum, Withania somnifera and Datura metel were tested against Alternaria alternata, Aspergillus flavus, Aspergillus ochraceus, Fusarium gramineorum and Penicillium citrinum by using 96-well plate method. Leaf extract showed remarkable activity against tested fungi at different concentrations as Minimum Inhibitory Concentration. The leaf extract of Calotropis procera and Azadirachta indica, have good potential antifungal properties against different mycotoxin producing fungi.

STUDY ON DEVELOPMENT OF PASTA PRODUCT WITH INCORPORATION OF OCIMUM SANCTUM (BASIL) TO ENHANCE THE SENSORY AND NUTRITIONAL QUALITY

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
PIYUSH MISHRA ◽  
DEVENDRA KUMAR BHATT
Keyword(s):  
Ambient Temperature ◽  
Shelf Life ◽  
Nutritional Quality ◽  
Leaf Extract ◽  
Storage Stability ◽  
Natural Antioxidants ◽  
Sensory Properties ◽  
Ocimum Sanctum ◽  
Leaf Powder

Pasta was prepared by incorporation of Ocimum sanctum (Basil) for better textural and sensory properties. The pasta was incorporated with the leaf extract of Ocimum sanctum at different concentrations of control, 5, 10, and 15.The natural antioxidants present in the O. sanctum leaf powder that was incorporated in the fruit leather showed extended shelf-life over three months when compared with control, without any added preservative at ambient temperature. Also the nutritional stability of the product was studied under two flexible packages of polypropylene and polyester out of that the products packed in polypropylene showed better storage stability .

Appraisal of innovative phytosynthetic CdO NPs from leaf extract of Ocimum Sanctum and their bactericidal activity against different strains

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Aarth R ◽  
Sudha A P ◽  
Sujatha B ◽  
Sowmya Lakshmi K
Keyword(s):  
Leaf Extract ◽  
Cadmium Oxide ◽  
Morphological Properties ◽  
Precipitation Method ◽  
Ocimum Sanctum ◽  
Oxide Nanoparticles ◽  
Average Crystalline Size ◽  
Face Centered Cubic ◽  
Bacterial Strains ◽  
X Ray

The phytosynthesis of n-type Cadmium Oxide Nanoparticles reduces the toxicity of the substance and makes it Eco-friendly. This Eco-friendly biosynthesis of CdO NPs was synthesized for the first time from the Queen of herbs, Ocimum Sanctum (holy basil).The biosynthesized Cadmium oxide was prepared using Ocimum leaf extract as a reductant and Cadmium Chloride and hydroxide as cadmium and oxide source materials by Co- Precipitation method. Thus obtained Cadmium Oxide Nanoparticles were characterized by different techniques such as X-ray diffraction (XRD), Fourier Transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM),Energy dispersive X-ray spectroscopy(EDS) to study the structural and morphological properties. XRD pattern exhibited the formation of face centered cubic structure of CdO NPs with an average crystalline size of 11.5nm .The chemical bond formation of CdO NPs were confirmed by FTIR spectrum in the range of (400-4000cm-1). The SEM micrographs revealed the predominant formation of Cauliflower shape with a particle size in the range of 61-142nm. The high purity of the biosynthesized nanoparticles were confirmed by EDS analysis. Further it was tested against gram positive and gram negative bacterial strains and showed significant antibacterial activity. This biosynthetic research study opens an innovative window to progress our understanding of how CdO NPs shows resistance to different bacterial strains.

Enhanced Anticancer Activity and Apoptosis Effect of Bioactive Compound Quercetin Extracted from Ocimum sanctum Leaves

2020 ◽  
Vol 10 ◽  
Author(s):  
Amutha Santhanam ◽  
Naveen Kumar Chandrasekharan ◽  
Rajangam Ilangovan
Keyword(s):  
Cell Cycle ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Leaf Extract ◽  
Nickel Nanoparticles ◽  
Bioactive Compound ◽  
Cell Cycle Analysis ◽  
Ocimum Sanctum ◽  
Sem Images ◽  
Ft Ir

Background: The occurrence of Cancer results in cellular changes that causes the uncontrolled growth and division of cells. Apoptosis removes cells during development and eliminates the potentially cancerous cells. The bioactive compounds present in the herbal plant shows cytotoxic activity that result in apoptosis. The traditional herbal plants are used world-wide both in allopathy and other traditional ways. Objective: The main objective of this study is to extract the bioactive compound Quercetin from the medicinally significant plant Ocimum sanctum and also to develop nanomedicine as Qu-PEG-NiGs. Materials and Methods: Leaf extract of the medicinally significant plant Ocimum sanctum (O. sanctum) has been used for the synthesis of nickel nanoparticles (NiGs) and extraction of quercetin (Qu). The ethanolic extract of Ocimum sanctum is added to 1 mM Nickel Nitrate (Ni(NO3)2) and stirred for 3 hrs at RT and dried at 60°C for 3hrs and calcinated at 400°C for 2hrs and characterized using Uv-Vis Spectrophotometer, FT-IR, SEM, DLS and Zeta potential. The Quercetin is isolated from Ocimum sanctum leaf extract using the reflux condenser method. The bio-polymer is being PEG-coated over NiGs and Quercetin is loaded into it. The apoptosis activity using MCF-7 cells is performed with Qu-PEG-NiGs. The purity of Quercetin is characterized using HPLC. In order to analyse apoptosis efficiency, MTT assay, Reactive Oxygen Species (ROS), Cell cycle analysis has been performed. Results: The NiGs absorption spectrum gives a peak at 408nm. The FT-IR confirms the presence of particular functional groups shifting from the compound NiGs and then coated with PEG-Qu-NiGs. The SEM images show the size of NiGs ranging from 27.3 nm to 40.4 nm with varied morphology such as hexagonal and other irregular shapes. The presence of Quercetin extracted from the leaf powder is approximately 1.5 mg/g. The ROS results show the Qu-PEG-NiGs induced efficiency of the apoptosis, while the increased concentrations promote ROS and lead to activation of the apoptosis. The cell cycle analysis has shown the cytotoxic effect. Conclusion: PEG-coated nickel nanoparticles can be used as a promising chemotherapeutic agent against MCF7 breast cancer cells. It is the evidence to further studies for evaluating Qu-PEG-NiGs anticancer activity on different types of cancer cells.

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