Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

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Chemical characterization and in vitro biological activity of Cymbopogon citratus extracts against Haemonchus spp. and Trichostrongylus spp. nematodes from sheep

Parasitology ◽

10.1017/s0031182020001432 ◽

2020 ◽

Vol 147 (13) ◽

pp. 1559-1568

Author(s):

Letícia Oliveira da Rocha ◽

Gloria Cristina da Silva Lemos ◽

Ivo José Curcino Vieira ◽

Raimundo Braz-Filho ◽

Silvério de Paiva Freitas ◽

...

Keyword(s):

Biological Activity ◽

Chemical Characterization ◽

Gastrointestinal Nematodes ◽

The Body ◽

Intestinal Cells ◽

Phytochemical Analysis ◽

Cymbopogon Citratus ◽

Thin Sections ◽

Methanolic Extracts

AbstractMedicinal plants have been the focus of several studies due to their nematicide properties which can be used to control nematodes in sheep. No study has examined the morphological effects of Cymbopogon citratus on nematodes. Thus, this study evaluated the chemical composition, nematicidal activity and effects of C. citratus extracts on the morphology of eggs and infective larvae (L3) of sheep. Aqueous and methanolic extracts and fractions of C. citratus were obtained and analysed in vitro. The C. citratus extracts were effective against Haemonchus spp. and Trichostrongylus spp. larvae and eggs. Ten fractions were obtained from C. citratus, six of which had high ovicidal activity at 1000 μg mL−1, and two fractions had high activity at all tested concentrations. The phytochemical analysis identified the presence of compounds such as terpenoids, various ketones, esters, and fatty acids. The ultrastructural analysis showed deformations of the cuticle and wilting along the body of the nematodes at all concentrations. The muscular layer, intestinal cells and the mitochondria profile showed damage compared to the typical pattern. Ultra-thin sections of eggs treated with methanolic fractions of C. citratus presented modifications. This study showed the biological activity and effects of C. citratus on the gastrointestinal nematodes in sheep.

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RAPID IN VITRO CALLOGENESIS AND PHYTOCHEMICAL SCREENING OF LEAF, STEM AND LEAF CALLUS OF MUSSAENDA FRONDOSA LINN. - A MEDICINAL PLANT.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.17527 ◽

2017 ◽

Vol 10 (6) ◽

pp. 81 ◽

Cited By ~ 1

Author(s):

Manasa Dj ◽

Chandrashekar Kr ◽

Bhagya N

Keyword(s):

Methanolic Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Leaf Explants ◽

Reducing Power ◽

Callus Cultures ◽

Phytochemical Analysis ◽

Qualitative And Quantitative

Objective: To standardise the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem and callus cultures.Methods: The leaf explants were inoculated onto MS medium supplemented with varying concentrations of growth regulators such as 2, 4 - D, NAA, BAP, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoids and alkaloids contents of leaf, stem and callus were tested by standard methods. The antioxidant activities were investigated using DPPH radical scavenging method and reducing power assay. The anti - inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable and fast growing callus was obtained on the medium enriched with NAA (2mg/l) + Kn (4mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10 ± 1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of Quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10g of extract). The methanolic leaf extract exhibited highest free radical scavenging activity with IC50 value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%).Conclusion: The present preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.

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Phytochemical and In-vitro Antioxidants Potential of Aristolochia Bracteolata Root Extract

Journal of Complementary and Alternative Medical Research ◽

10.9734/jocamr/2021/v13i330224 ◽

2021 ◽

pp. 1-7

Author(s):

H. M. Goji ◽

M. S. Nadro

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Phytochemical Analysis ◽

Root Extract ◽

Root Extracts ◽

Qualitative And Quantitative ◽

Oxidative Stresses ◽

Total Antioxidant ◽

Aristolochia Bracteolata

The study investigated the phytochemical and in-vitro antioxidants potential of Aristolochia bracteolata root extracts. Qualitative and quantitative phytochemical analysis of the plant extract and fractions were carried out. The total antioxidant and free radical scavenging activity of extract and fractions were measured by 1, 1-diphenyl-2-picryl-hydrazyl, FRAP and TBARS methods. Results obtained showed that total saponins (10.10 ± 0.05d) as well as antioxidant activity were found to be significantly (p< 0.05) higher in the methanol extract compared to the fractions. From the findings above, Aristolochia bracteolata root contains bioactive phytochemicals that might be helpful in preventing or slowing the progress of various oxidative stresses.

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Phytochemical analysis and in vitro evaluation of the biological activity against herpes simplex virus type 1 (HSV-1) of Cedrus libani A. Rich.

Phytomedicine ◽

10.1016/j.phymed.2007.03.013 ◽

2008 ◽

Vol 15 (1-2) ◽

pp. 79-83 ◽

Cited By ~ 29

Author(s):

Monica Rosa Loizzo ◽

Antoine Saab ◽

Rosa Tundis ◽

Giancarlo A. Statti ◽

Ilaria Lampronti ◽

...

Keyword(s):

Biological Activity ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Phytochemical Analysis ◽

In Vitro Evaluation ◽

Simplex Virus

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Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Kalanchoe Pinnata (Lam.) Pers. Leaf Extracts

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1.2225 ◽

2019 ◽

Vol 9 (1) ◽

pp. 220-224 ◽

Cited By ~ 1

Author(s):

Santosh Kumar Singh ◽

Jay Ram Patel ◽

Arvind Dangi

Keyword(s):

Quantitative Analysis ◽

Total Phenolics ◽

Ethanolic Extract ◽

Aluminium Chloride ◽

Assay Method ◽

Phytochemical Analysis ◽

Dpph Assay ◽

Qualitative And Quantitative ◽

Kalanchoe Pinnata

Kalanchoe pinnata (Lam.) Pers. has many pharmacological properties such as anticancer, antidiabetic, insecticidal, antimicrobial, anti-urolithiatic, etc. The plant leaves are used as ethno medicine traditionally. Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate physicochemical, qualitative and quantitative phytochemical analysis and in vitro antioxidant activities of leaf of Kalanchoe pinnata collected from Bhopal region of Madhya Pradesh.The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by FolinsCiocalteau reagent method and Aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the leaf was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of leaves ethanolic extract was (4.63mg/100mg), followed by flavonoids (2.03mg/100mg). The activities of ethanolic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 24.44 and 49.81μg/ml respectively. These studies provided information for standardization and correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine. Keywords: Kalanchoe pinnata, Physicochemical, Qualitative, Quantitative phytochemical, Antioxidant

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In Vitro Phytochemical Screening and Antioxidant Activity of Jamun (Eugenia jambolana Linn) Plants Parts Collected from Lahore, Pakistan

Biochemistry and Modern Applications ◽

10.33805/2638-7735.118 ◽

2019 ◽

pp. 20-23

Author(s):

Nureen Zahra ◽

Momna Nadir ◽

Arif Malik ◽

Ayesha Shaukat ◽

Asia Parveen ◽

...

Keyword(s):

Antioxidant Activity ◽

Quantitative Estimation ◽

Research Work ◽

Radical Scavenging ◽

Solvent System ◽

Phytochemical Analysis ◽

Qualitative And Quantitative ◽

Eugenia Jambolana ◽

Water Solvent

Our research work based on the qualitative and quantitative phytochemical studies including the antioxidant activity of extracts (ethanolic and aqueous) obtained from leaf, stem and seed of Eugenia jambolana plant. Qualitatively phytochemical analysis was done by biochemical testing, quantitative estimation was taken spectrophotometrically and antioxidant activity of extracts was tested by DPPH radical scavenging. Qualitative and quantitative outcomes of our study explain that ethanol is applicable solvent system that comprises high quantity of bioactive compounds than water solvent system. Antioxidant activity revealed that ethanolic leaf, seed and stem extracts removed free radicals 68%, 98% and 33% respectively in contrast with water extracts of leaf, seed and stem 5.09%, 1.25% and 7.26% respectively. Ascorbic acid was taken as standard that kills 97% free radical species. Extracts in ethanol solvent system displayed protruding antioxidant activity and also contains high amount of phtyo-chemicals.

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Volatiles from Steam-distilled Leaves of Some Plant Species from Madagascar and New Zealand and Evaluation of Their Biological Activity

Natural Product Communications ◽

10.1177/1934578x1000501123 ◽

2010 ◽

Vol 5 (11) ◽

pp. 1934578X1000501 ◽

Cited By ~ 1

Author(s):

Rosaria Costa ◽

Francesco Pizzimenti ◽

Francesca Marotta ◽

P. Dugo ◽

Luca Santi ◽

...

Keyword(s):

Biological Activity ◽

New Zealand ◽

Quantitative Analysis ◽

Plant Species ◽

Cinnamomum Camphora ◽

Qualitative And Quantitative Analysis ◽

Qualitative And Quantitative ◽

Aerial Parts ◽

Leptospermum Scoparium

Steam-distilled aerial parts of Ravensara Aromatica and Cinnamomum camphora from Madagascar and Leptospermum scoparium from New Zealand have been subjected to qualitative and quantitative analysis by means of GC techniques. This allowed the elucidation of conflicting data present in the available literature for these species. Also, the biological activity in vitro was evaluated by measuring MICs and GIZs.

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Comparative analysis of Qualitative and Quantitative Phytochemical screening, in vitro antioxidant and antibacterial activity of Mangifera indica and Artocarpus heterophyllus

Journal of University of Shanghai for Science and Technology ◽

10.51201/jusst/21/06468 ◽

2021 ◽

Vol 23 (06) ◽

pp. 1554-1568

Author(s):

T. Manimekalai ◽

◽

Dr. P. Chitra ◽

Keyword(s):

Mangifera Indica ◽

Phytochemical Screening ◽

Flavonoid Content ◽

Leaf Extracts ◽

Artocarpus Heterophyllus ◽

Qualitative And Quantitative ◽

Phenolic And Flavonoid ◽

Different Parts ◽

Screening In Vitro

Medicinal plants have known to play a wide role in the development of human culture. Artocarpus heterophyllus and Mangifera indica are the well-known evergreen tropical fruiting tree families abundantly grown in Asia. Different parts of both the trees such as bark, leaves, fruits, and flowers are used in traditional medicine. The present study aims to screen the qualitative and quantitative phytochemical present, in-vitro antioxidant, and anti-microbial activity of Artocarpus heterophyllus and Mangifera indica leaf extracts and combined extract of both the leaves. The qualitative phytochemical results indicated the presence of phytochemicals such as alkaloids, flavonoids, phytosterols, tannins, and saponins. The quantitative phytochemical results revealed that M.indica has high phenolic and flavonoid content. The Combined extract showed significant antioxidant activity when compared to both the extracts. All the extracts showed significant antimicrobial activity.

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