Optimization of protein and tannin extraction in Moringa oleifera leaf as
antioxidant source

Moringa oleifera contains high nutritional and bioactive compounds that have the potential as a source of antioxidants. The main objective of this study was to obtain optimal conditions in the extraction process of Moringa leaf, maintain protein and antioxidant activity, and reduce tannin. It is carried out through the response surface method which consists of two factors. The first factor (X1) was the extraction temperature (°C) which contains three levels, namely 70, 80, and 90°C. The second factor (X2) was time (mins) consisting of three levels, namely 5, 10, and 15 mins. The parameters in this study were total protein, antioxidant activity, and total tannin. The results revealed that the optimum condition for Moringa leaf extraction is found to be temperature 80.54°C and a time of 12.19 mins. In that treatment, the total protein is 17.4594%, antioxidant activity is up to 10.2629 µg/mL, and tannin is 7.853% with the desirability of 0.792 or 79.2%.

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Optimization of banana crop by-products solvent extraction for the production of bioactive compounds

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-01703-7 ◽

2021 ◽

Author(s):

Sara Díaz ◽

Antonio N. Benítez ◽

Sara Ramírez-Bolaños ◽

Lidia Robaina ◽

Zaida Ortega

Keyword(s):

Antioxidant Activity ◽

Aqueous Ethanol ◽

Ethanol Concentration ◽

Extraction Process ◽

Total Phenol Content ◽

Optimal Conditions ◽

Satisfactory Description ◽

Optimized Conditions ◽

By Products ◽

Banana Crops

AbstractThe aim of this work is the optimization of phenolic compound extraction from three by-products of banana crops (rachis, discarded banana, and banana’s pseudostem pulp), as a way to valorize them through a green extraction process. The influence of the temperature and aqueous ethanol concentration (Et-OH) on extract properties (total phenol content (TPC) and antioxidant activity) was firstly analyzed. 78 ℃ and ethanol concentrations close to 50% yielded the best results for the three materials. The equations obtained by the response surface methodology gave a satisfactory description of the experimental data, allowing optimizing the extraction conditions. Under optimized conditions, time influence was then assessed, although this parameter seemed not influence results. Among the three by-products, rachis extract (60% Et-OH, 78 ℃, and 30 min) presented the highest TPC (796 mg gallic acid/100 g of dried material) and antioxidant activity (6.51 mg Trolox equivalents/g of dried material), followed by discarded banana, and pseudostem pulp. Under the optimal conditions, experiments were performed at a larger scale, allowing to determine the extraction yields (EY) and to characterize the extracts. The highest EY was obtained for the rachis (26%), but the extract with the highest activity was obtained for discarded banana (50% Et-OH, 78 ℃, and 60 min), which presented a TPC of 27.26 mg/g extract corresponding to 54.59 mg Trolox equivalents/g extract. This study contributes to the valorization of banana crops residues as a source of polyphenolic compounds with bioactive functions that can be extracted under economic extraction conditions. Graphical abstract

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Extraction Process of Polyphenols from Soybean (Glycine max L.) Sprouts: Optimization and Evaluation of Antioxidant Activity

Processes ◽

10.3390/pr7080489 ◽

2019 ◽

Vol 7 (8) ◽

pp. 489 ◽

Cited By ~ 5

Author(s):

Xuan Tien Le ◽

Vo Luu Lan Vi ◽

Tran Quoc Toan ◽

Long Giang Bach ◽

Tran Thanh Truc ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethanol Concentration ◽

Dry Weight ◽

Extraction Process ◽

Extraction Time ◽

Extraction Temperature ◽

Polyphenol Content ◽

Experimental Conditions ◽

Total Polyphenol ◽

Total Polyphenol Content

This research aimed to optimize the total polyphenol content (TPC) extracted from soybean sprout powder under different experimental parameters, including ethanol concentration (60–100% v/v), extraction temperature (40–80 °C), extraction time (15–150 min), material:solvent ratio (1:4–1:10 g/mL), the number extraction cycles (1, 2 and 3 times), the age of sprout (0–7 days), and the used part of the sprout (cotyledon, hypocotyl, or radicle). The obtained results were used in response surface methodology, in combination with a central composite design, to model the total polyphenol content (TPC) with respect to three variables, including ethanol concentration, extraction temperature, and material:solvent ratio. The experimental conditions for optimal recovery of TPC consisted of ethanol concentration of 88% (v/v), extraction temperature of 59 °C, material:solvent ratio of 1:6.5 g/mL, extraction time of 60 min, and 2 cycles of maceration. In addition, for maximal TPC, the sprout should undergo the germination of 5 days and the radicle fraction should be used. Based on the suggested optimum conditions, the obtained and verified TPC was 19.801 mg genistein (GE)/g dry weight (d.w.). The obtained dried extract also exhibited low antioxidant activity.

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Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.814.527 ◽

2019 ◽

Vol 814 ◽

pp. 527-531

Author(s):

Trieu Tuan Anh ◽

Luu Tu Hao ◽

Long Giang Bach ◽

Duy Chinh Nguyen ◽

Tri Duc Lam

Keyword(s):

Antioxidant Activity ◽

Extraction Process ◽

Extraction Yield ◽

Total Phenolic ◽

Extraction Temperature ◽

Total Phenolic Compounds ◽

Polyphenol Content ◽

Total Polyphenol ◽

Herbal Plants ◽

Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

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Influence of the CO2 Quality in the Antioxidant Activity of Rosemary Extracts Dearomatized by Supercritical Fluid Extraction

Food Science and Technology International ◽

10.1177/108201320100700212 ◽

2001 ◽

Vol 7 (2) ◽

pp. 177-182 ◽

Cited By ~ 4

Author(s):

E. Ibanez ◽

S. Lopez-Sebastian ◽

J. Fernandez ◽

J. Tabera ◽

J. M. Bueno ◽

...

Keyword(s):

Antioxidant Activity ◽

Experimental Design ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Sunflower Oil ◽

Extraction Process ◽

Taguchi Experimental Design ◽

Optimal Conditions ◽

Fluid Extraction

The effect of the CO2 quality (mainly related to CO2 content) in the antioxidant activity of rosemary extracts deodorized by using a supercritical fluid extraction process is analyzed. A Taguchi experimental design was used to study the influence of the CO2 quality on the antioxidant activity of the rosemary dearomatized extracts. The antioxidant activity of the rosemary extracts obtained in optimal conditions was tested with sunflower oil.

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Optimization of Phenolic Compound Extraction from Chinese Moringa oleifera Leaves and Antioxidant Activities

Journal of Food Quality ◽

10.1155/2019/5346279 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Beibei Zhao ◽

Jiawen Deng ◽

Hua Li ◽

Yaqiang He ◽

Tao Lan ◽

...

Keyword(s):

Antioxidant Activity ◽

Moringa Oleifera ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Ultrasonic Extraction ◽

Extraction Temperature ◽

Moringa Oleifera Leaves

Rich in phenolic compounds, Moringa oleifera leaf extract (ME) exhibits significant antioxidant activity both in vitro and in vivo. ME has already been widely used in fields of medicine, functional food, and cosmetics. Ultrasonic extraction (UE) method has been improved to be one of the most effective ways to extract phenols from M. oleifera leaves. The purpose of this study was to optimize ultrasonic extraction of phenols by response surface methodology (RSM). Four parameters were discussed, such as ethanol concentration, solvent-sample ratio, extraction temperature, and extraction time. Also, purification methods of the crude ME by organic solvent extraction and column chromatography were examined. Antioxidant activities of ME and each fraction were evaluated by DPPH, ABTS, and hydroxy radical-scavenging activities and reducing power. The phenol content of the purified ME reached up to 962.6 mg RE/g, extremely higher than the crude extract 107.22 ± 1.93 mg RE/g. The antioxidant activity of the purified ME was also significantly improved. Furthermore, phenols were identified by using the HPLC-MS method, and the results showed that there were 6 phenolic acids and derivatives and 7 flavonoids in ME. Quercetin-3-O-β-D-glucoside isolated from ME showed excellent DPPH and ABTS radical-scavenging abilities, which were comparable to VC.

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Optimized Extraction of Polysaccharides from Bergenia emeiensis Rhizome, Their Antioxidant Ability and Protection of Cells from Acrylamide-induced Cell Death

Plants ◽

10.3390/plants9080976 ◽

2020 ◽

Vol 9 (8) ◽

pp. 976 ◽

Cited By ~ 2

Author(s):

Chen Zeng ◽

Shiling Feng

Keyword(s):

Folk Medicine ◽

Extraction Process ◽

Optimal Conditions ◽

Antioxidant Ability ◽

Hep G2 ◽

Hep G2 Cells ◽

Surface Method ◽

C 30 ◽

Sample Ratio ◽

Great Protection

Bergeniaemeiensis is a traditional herb in Chinese folk medicine. Most related studies are focused on the bioactivity of bergenin, neglecting other bioactive compounds. In our previous work, polysaccharides were identified in B. emeiensis rhizome. To evaluate the extraction process and the antioxidant ability of these polysaccharides, a response surface method and antioxidant assays were applied. The results showed that the yield of polysaccharides was highly affected by extraction time, followed by temperature and solvent-to-sample ratio. Under the optimal conditions (43 °C, 30 min and 21 mL/g), the yield was 158.34 ± 0.98 mg/g. After removing other impurities, the purity of the polysaccharides from B. emeiensis (PBE) was 95.97 ± 0.92%. The infrared spectrum showed that PBE had a typical polysaccharide structure. Further investigations exhibited the PBE could scavenge well DPPH and ABTS free radicals and chelate Fe2+, showing an excellent antioxidant capacity. In addition, PBE also enhanced the cell viability of HEK 239T and Hep G2 cells under acrylamide-exposure conditions, exhibiting great protection against the damage induced by acrylamide. Therefore, PBE can be considered a potential natural antioxidant candidate for use in the pharmaceutical industry as a health product.

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Study on the Optimization for the Extraction Process of Andrographolide by Ultrasonic Assisted Extraction

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset21824 ◽

2021 ◽

pp. 08-17

Author(s):

KiBong Kim ◽

IlJin Kim ◽

SongLok Uh ◽

YongIl Seo ◽

CholKwang Li ◽

...

Keyword(s):

Optimum Condition ◽

Response Surface ◽

Folk Medicine ◽

Solution Concentration ◽

Extraction Process ◽

Extraction Temperature ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction ◽

Medical Role

Because andrographis has a long history of folk medicine in Southeast Asian countries with antibacterial, antipyretic, antineoplastic, anti-inflammatory, cardioprotective effect and so on, the areas where andrographolia is introduced and cultivated are mainly concentrated. Espically andrographolide, neoandrographolide, andrographiside, homoandrographolide, andrographane, andrographanin, andrographone and andrographosterol that is concentrated in the leaves of the plant play the main medical role of andrographis. Of the above chemicals, andrographolide is the major bioactive constituent responsible for variety of activities. Leaves and stems of plant are used for extracting active phytochemicals; roots are used rarely. In this study, improved the extractability of andrographolide from the A. paniculata by ultrasonic assisted extraction, the optimum condition for extracted the andrographolide is determined by response surface experiment and detected the extractability of andrographolide under the optimum condition. By single factor analysis and response surface experiments, the optimum conditions for extracted the andrographolide were the extraction solution concentration 62.8%, the ultrasonic extraction time 59min, the ratio of solid to liquid 1:10.5 and the extraction temperature 62?. Investigate the andrographolide extractability in the optimun extraction condition by HPLC were increased from 2.27% to 3.28%.

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Extraction yield, antioxidant activity and total phenolic content of Mimusops elengi L. fruit

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/922/1/012021 ◽

2021 ◽

Vol 922 (1) ◽

pp. 012021

Author(s):

Z Zaidiyah ◽

M G A Ghifari ◽

Y Abubakar

Keyword(s):

Antioxidant Activity ◽

Extraction Process ◽

Extraction Yield ◽

Effect Of Temperature ◽

Total Phenolic ◽

Extraction Temperature ◽

Fruit Extract ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Mimusops Elengi

Abstract Mimusops elengi L. fruit or known as Bakul fruit is believed to have a lot of benefit due to its flavonoid group compound that can have antioxidant effects. This study aimed to determine the effect of temperature and ratio between material and solvent in the extraction process on the antioxidant activity and content of phenolic compounds in Mimusops elengi L. fruit extract. This research used factorial completely randomized design with 2 factors : extraction temperature (45¼C, 60¼C, and 75¼C) and ratio of the material and solvent (1:4, 1:6, and 1:8) with 3 replications. The extraction yield, total phenolic contents and antioxidant activity of Mimusops elengi L. fruit extract were examined. Furthermore, the application of extraction temperature in 75¼C with ratio 1:8 material to solvent (w/v) has the highest extraction yield, total phenolic contents and antioxidant activity compared to others treatments.

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Extraction Process Optimization of Endo-Polysaccharides from Phellinus igniarius

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.881-883.347 ◽

2014 ◽

Vol 881-883 ◽

pp. 347-350

Author(s):

Yan Xu ◽

Hui Cao ◽

Shuang Shuang Gu ◽

Sha Sha Wang ◽

Jun Wang ◽

...

Keyword(s):

Trichloroacetic Acid ◽

Ethanol Concentration ◽

Removal Rate ◽

Extraction Process ◽

Extraction Time ◽

Extraction Temperature ◽

Optimal Conditions ◽

Phellinus Igniarius ◽

Maximal Yield ◽

Final Ethanol Concentration

Polysaccharides ofPhellinus igniariushave remarkable bioactivities and attracted considerable attention. In this study, extraction process of endo-polysaccharides fromP. igniariuwas investigated. The extraction conditions including ratio of sporocarp to water, extraction time, extraction temperature, extraction times were optimized. The experimental results indicated that the maximal yield of endo-polysaccharides fromP. igniariuwas 2.93 %, and the optimal conditions were as follows: ratio of sporocarp to water 1:30, extraction time 2 h, extraction temperature 90 °C, extraction twice. The best final ethanol concentration was 80 %. In addition, protein removal rate could reach 90.6 % using trichloroacetic acid method in extracting polysaccharides process.

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Potential of Germination in Selected Conditions to Improve the Nutritional and Bioactive Properties of Moringa (Moringa oleifera L.)

Foods ◽

10.3390/foods9111639 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1639

Author(s):

Karín E. Coello ◽

Juana Frias ◽

Cristina Martínez-Villaluenga ◽

María Elena Cartea ◽

Rosaura Abilleira ◽

...

Keyword(s):

Antioxidant Activity ◽

Functional Foods ◽

Moringa Oleifera ◽

Nutritional Composition ◽

Aminobutyric Acid ◽

Optimal Conditions ◽

Bioactive Properties ◽

Response Optimization ◽

Maximum Content

Moringa oleifera L. is greatly appreciated for its high content of phytochemicals. Although most parts of moringa tree have been widely studied, seeds remained scarcely explored. The first goal of this study was to investigate the effectiveness of germination to improve the nutritional composition (proximate composition and levels of vitamins B1 and B2), content of bioactive compounds (glucosinolates, phenolics and γ-aminobutyric acid, GABA) and antioxidant activity of moringa seed. Germination improved protein, fat, fiber, riboflavin, phenolics, some individual glucosinolates (GLS) and GABA contents, as well as the antioxidant potential in moringa sprouts, but the extent of the improvement depended on germination conditions. The second objective of this work was to identify the optimal germination conditions to maximize nutritional and bioactive quality of moringa by applying multi-response optimization (response surface methodology, RSM). RSM models indicated that 28 °C and 24 h were the optimal conditions to enhance the accumulation of riboflavin, phenolics and antioxidant activity of sprouts, while the highest GABA and total GLS contents were observed at 36 °C for 96 h and thiamine achieved the maximum content at 36 °C for 24 h. These results show that moringa sprouts are promising functional foods that might be also used as ingredients for the elaboration of novel foodstuffs.

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