THE PREDICTION OF THE ANTIOXIDATIVE ACTIVITY OF GROSSULARIA RECLINATA LEAVES

At present, in order to expand the raw material base, modern phyto-production is most interested in the use of medicinal plants' organs that were not previously used, but containing flavonoids. The Grossularia reclinata leaves, which are a cheap and affordable plant material, can become a promising source for the creation of phytopreparations containing flavonoid compounds. The aim of the study was to identify and quantify the sum of flavonoids' substances in terms of rutin in the Grossularia reclinata leaves. Material and methods. Qualitative reactions were selected to confirm the presence of flavonoids' substances; differential spectrophotometry was used for their quantitative assessment. Results. It was found that the total content of flavonoids in terms of rutin in alcohol extract from these leaves is on average 0.557 ± 0.0061%, which exceeds their content fruits that were used in medicine by 1.86 times. Comparative analysis of the flavonoids' sum of in the medicinal plants' leaves made it possible to determine that this group of biologically active substances in the Grossularia reclinata leaves is represented in greater quantities than in the motherwort herb (0.2%), wormwood (0.3%) and the tripartite series (0 ,five%). Conclusions: The results of the study allow us to consider this plant raw material promising for the production of new therapeutic and prophylactic drugs.

Comparative study of the elemental composition in raw materials of plants of genus Geum L.

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.2.21.07 ◽

2021 ◽

pp. 70-77

Author(s):

С. А. Козира ◽

Т. М. Гонтова ◽

С. І. Степанова ◽

В. П. Гапоненко ◽

В. В. Машталер ◽

...

Keyword(s):

Medicinal Plants ◽

Comparative Study ◽

Atomic Absorption ◽

Elemental Composition ◽

Raw Materials ◽

Biologically Active ◽

Raw Material ◽

Material Base ◽

Plant Raw Materials ◽

Microelement Composition

Among the relevant for the modern pharmacy problems, there is a need for the rational complex use of known medicinal plants, as well as the search for new sources of natural biologically active compounds in order to expand the list of official medicinal plants and raw material base. Species of genus Geum L. contain a number of biological active substances and are promising sources of raw materials for the production of antimicrobial, astringent, anti-inflammatory, and hemostatic medicines. Pharmacological activity of these plants results from their multi-component chemical composition, including tannins, flavonoids, macro- and microelements and other compounds. The aim of thе study was to compare the elemental composition of herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. of Ukrainian flora. The objects of the study were the samples of herb and rhizome with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. (dried raw materials) which were collected in the Kharkiv region, Ruski Tishki village, in June-August 2018–2019. The determination of the qualitative composition and quantitative content of elements was carried out by atomic absorption spectroscopy with atomization in an air-acetylene flame using a spectrometer CAS 120 («Selmi», Sumy, Ukraine). In the studied samples, 19 elements were determined, including 5 macroelements and 14 microelements. The data about accumulation of elements in investigated herb of G. urbanum L., G. aleppicum Jacq., G. rivale L. can be arranged in descending order as follows: K > Ca > Si > Mg > P > Fe > Al > Na > Sr > Zn, and in rhizomes with roots as follows – K > Si > Ca > Mg > Fe > P > Al > Na > Sr > Zn. The content of toxic elements such as cobalt, cadmium, arsenic, and mercury was within the permissible limits for plant raw materials and food. For the first time, a comparative study of the macro- and microelement composition in herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. has been carried out. Using the method of atomic absorption spectral analysis, 19 elements have been identified and their content has been determined, among which К, Ca, Mg, Si, P, Fe, Al, Na, Mn, Zn predominantly accumulate.

Drinks based on milk whey using fruit syrup from wild plants

New Technologies ◽

10.47370/2072-0920-2021-17-2-33-39 ◽

2021 ◽

Vol 17 (2) ◽

pp. 33-39

Author(s):

T. B. Kolotiy ◽

Z. S. Kovalenko

Keyword(s):

Nutritional Value ◽

Rubus Idaeus ◽

Biologically Active ◽

Raw Material ◽

Wild Plants ◽

Material Base ◽

Viburnum Opulus ◽

Plant Materials ◽

Recently, there has been an increasing interest in whey drinks. It is possible to expand the range of whey drinks and regulate their nutritional value due to the wide use of a variety of fillers. Non-traditional local plant materials can be used as fillers. The products of processing (powders, juices, syrups) of fruits and berries are more and more widely used. The value of wild fruits, along with a pleasant taste, is determined by the content of vitamins, minerals, antioxidants and other biologically active substances that play an important role in human nutrition, regulate metabolic processes, and affect the functions of individual organs. The aim of the research is to study the effect of fruit syrups on the nutritional value of whey drinks. The fruits of the following wild plants are consid- ered as the objects: common viburnum (Viburnum opulus), cinnamon rosehip (Rosa cinnamomeal), raspberry (Rubus idaeus L.). The choice of raspberries, rose hips and viburnum as raw materials for enriching drinks is due to the high content of biologically active substances, such as vitamins C, B, E, a fairly large raw material base, environmental friendliness, and availability. The article considers the results of the use of fruit syrups obtained from rose hips, viburnum and raspberries in the preparation of whey drinks. The influence of the dosage of fruit syrups (5 %, 10 % and 15 %) on the organoleptic and physicochemical indicators of the quality of whey drinks has been established. The optimal dosages of syrups from fruits of wild plants in the preparation of whey drinks have been determined. Rosehip, viburnum and raspberry syrups have a high nutritional value, which will enrich whey drinks and give them a functional focus.

Grape pomace as a promising source of biologically valuable components

BIO Web of Conferences ◽

10.1051/bioconf/20213406002 ◽

2021 ◽

Vol 34 ◽

pp. 06002

Author(s):

Anastasia Tikhonova ◽

Natalia Ageeva ◽

Ekaterina Globa

Keyword(s):

Vitamin B1 ◽

Total Content ◽

Grape Pomace ◽

Pinot Noir ◽

Biologically Active ◽

Cabernet Sauvignon ◽

Grape Variety ◽

Hydroxybenzoic Acids ◽

Promising Source ◽

Experimental data on the content of biologically valuable components in grape pomace are presented. The presence of organic acids was revealed in an amount from 33.35 g/kg (Saperavi) to 108.2 g/kg (Rebo). The main acid is tartaric. Phenolic compounds have been identified: anthocyanins, flavones, flavan-3-ols, oxycinnamic and hydroxybenzoic acids, procyanidins. The main anthocyanins found in grape pomace are the 3-O-glycosides of malvidin, petunidin, cyanidin, peonidin and delphinidin. The highest total content of hydroxybenzoic acids was in the pomace of Pinot Noir grapes – 720 mg/kg, further in descending order of concentration followed by pomace from Saperavi grapes (708 mg/kg), Cabernet Sauvignon (681 mg/kg), Merlot (575 mg/kg) and Rebo (545 mg/kg). The highest total content of vitamins was found in the pomace of Rebo grapes – 639 mg/kg, the lowest – Merlot (471 mg/kg). In all samples, vitamin B1 prevails, then in decreasing order – ascorbic acid, vitamins B2, B3, B5, B7. Moreover, their concentrations vary significantly depending on the grape variety from which the pomace is obtained. The influence of the grape variety on the content of these biologically valuable components in the pomace has been established, which must be taken into account in the production of biologically active substances that increase the nutritional status of a person.

Extraction of valuable components from fruits of the hawthorn and barbaris of the North-West region

Известия СПбЛТА ◽

10.21266/2079-4304.2020.232.181-195 ◽

2020 ◽

Author(s):

И.А. Морозкова ◽

Н.А. Кутакова ◽

С.И. Третьяков ◽

Н.Н. Васильева

Keyword(s):

Climatic Factors ◽

Biologically Active ◽

Raw Material ◽

North West ◽

The North ◽

Cultivation Technology ◽

Degree Of Extraction ◽

Promising Source ◽

Плоды барбариса и боярышника содержат ценные биологически активные вещества: органические кислоты, сахара, дубильные вещества, антоцианы, аскорбиновую кислоту и другие компоненты. Количественное содержание этих групп соединений в данном материале зависит от эколого-географических, климатических факторов, условий выращивания, способа сушки и др. Сопоставление состава плодов, собранных в Дендросаду САФУ (г. Архангельск) в 2015–2018 гг., и основных климатических характеристик вегетационного периода показало, что влияние климата велико. Результаты исследования состава плодов нескольких видов барбариса и боярышника характеризуют их как ценный материал для более глубокого изучения и переработки. Как показывают имеющиеся данные, анализируемые вещества проявляют разнообразный спектр фармакологической активности: противовирусные, противовоспалительные, антимикробные, антидиабетические и противоопухолевые свойства. Для извлечения комплекса БАВ предложено использовать 70%-й этиловый спирт. Проведена оценка эффективности извлечения БАВ различными методами: метод настаивания, ультразвуковой обработки и экстракции в электромагнитном поле сверхвысоких частот (СВЧ), из них СВЧ-экстракция признана перспективной. Степень извлечения компонентов составила в среднем 50%. Плоды являются перспективными для использования в пищевой промышленности и для разработки новых лечебно-профилактических лекарственных средств на основе экстрактов. The fruits of barberry and hawthorn contain valuable biologically active substances: organic acids, sugars, tannins, anthocyanins, ascorbic acid and other components. The quantitative content of these groups of compounds in this raw material varies depending on ecological, geographical, climatic factors, the phase of plant growth, cultivation technology, drying method, etc. Comparison of the composition of the fruits collected in the Dendrosade of NArFU (Arkhangelsk) in 2015–2018 with the main climatic characteristics of the growing season showed that the influence of the climate is great. The results of a study the composition of the fruits of several types o of barberry and hawthorn characterize them as valuable material for a promising source for deeper study and processing. As the available data show, the analytes exhibit a diverse spectrum of pharmacological activity: antiviral, anti-inflammatory, antimicrobial, antidiabetic and antitumor properties . To extract the complex of biologically active substances, it is proposed to use 70% ethanol. The efficiency of extraction of biologically active substances by various methods was evaluated: the method of insisting, ultrasonic treatment and extraction in an electromagnetic field of microwave frequencies, of which microwave extraction was recognized as promising. The degree of extraction of the components averaged 50%. The fruits are promising for use in the food industry and for the development of new therapeutic and preventive medicines based on extracts.

Investigation of Walnut Herbal Drug Raw Material

Drug development & registration ◽

10.33380/2305-2066-2019-8-4-38-46 ◽

2019 ◽

Vol 8 (4) ◽

pp. 38-46

Author(s):

N. V. Chebyshev ◽

A. V. Strelyaeva ◽

D. I. Lezhava ◽

A. N. Luferov ◽

N. V. Bobkova ◽

...

Keyword(s):

Mass Spectrometry ◽

Medicinal Plant ◽

Raw Materials ◽

Juglans Regia ◽

Biologically Active ◽

Raw Material ◽

Alcohol Extract ◽

Plant Raw Materials ◽

Introduction. Walnut (Juglans regia L.) is a medicinal plant containing a large number of biologically active substances. A number of authors propose to use walnut leaves as medicinal raw materials, in which juglone, flavonoids, tannins, ascorbic acid and other substances are identified. Currently, a standardization technique for this type of raw material has been developed and proposed. However, the medicinal plant walnut can be a source of other types of raw materials, such as milky-waxed walnuts, walnut bark, and walnut partitions. These types of medicinal raw materials contain a large number of biologically active substances and have a great potential for pharmacological activity. Therefore, the study with the subsequent standardization of medicinal plant material of walnut fruits in the stage of milk-wax ripeness and walnut bark is relevant.Aim. The aim of the study is to study the external signs, microscopy of raw walnut bark and walnut fruit in the stage of milky-wax ripeness and chemical composition of alcohol extract from these types of raw materials.Materials and methods. Chromato-mass spectrometry and TLC were used in the work.Results and discussion. When describing external signs and microscopy, diagnostic signs of both whole and crushed raw materials, and powder of walnut bark, and fruits of walnut in the stage of milky-wax ripeness were revealed. Alcohol extract obtained from medicinal plant raw materials walnut bark is a clear brown liquid. Alcohol extract obtained from medicinal plant raw materials of walnut fruit at the stage of milky-wax ripeness is a transparent, green-brown liquid with a fragrant odor.Conclusion. Gallic acid was identified by TLC in walnut bark. Chromato-mass spectrometry in alcohol extraction from the fruit of walnut in the stage of milky-wax maturity was able to identify 17 compounds belonging to different classes of biologically active substances. Sugars, flavonoids, coumarin phenolic compounds, organic acids were identified. In alcoholic extraction from the bark of a walnut, marker compounds have been determined, the combination of which makes it possible to quickly identify this type of raw material using chromatography-mass spectrometry.

CHARACTERISTICS OF HORNY ALTAI PEATS AND THEIR BIOLOGICAL ACTIVITY IN PLANT TISSUE CULTURE

chemistry of plant raw material ◽

10.14258/jcprm.2019035132 ◽

2019 ◽

pp. 261-268

Author(s):

Lidiya Ivanovna Inisheva ◽

Ol'ga Aleksandrovna Rozhanskaya ◽

Galina Vasil'yevna Larina

Keyword(s):

Tissue Culture ◽

Organic Matter ◽

Biological Activity ◽

Plant Tissue ◽

Raw Materials ◽

Biologically Active ◽

Raw Material ◽

Material Base ◽

Gorny Altai ◽

The search for new raw materials of biologically active substances of natural origin is an urgent task for the modern period. Peat in this respect is a relatively cheap and almost unlimited raw material base. Peat of swamps can be used widely in agriculture for receipt of biologically active substances are of great interest in the territory of the Gorny Altai. The purpose of this work is to study the composition of organic matter of peats of the Gorny Altai, to choose peat raw materials for biologically active production and to study their biological activity. The object of the study was 46 swamps of the Gorny Altai. The following analyses were carried out in peats: botanical composition, degree of decomposition, ash content, group composition of peat organic matter. The composition of humic acids (ha) was analyzed by IR spectroscopy. For determine of the biological activity of humic acids was used plant tissue cultured . The results of the research allowed to distinguish peats by the content of HA: buckbean peat (47.0% of HA), wood peat (50.0% of HA), fern peat (55.0% of HA), grass peat (30.0–45.0% of HA), sedge peat (5.6–58.0% of HA), grass-buckbean peat (43.0–56.5% of HA) and to outline the raw material base for the production of BAS – peatland Turochak. According to the optimal characteristics of HAs, a sample was taken from a depth of 325-375 cm, HA was isolated. The biological activity In humic acid was determined with the use of plant tissue culture. High biological activity are proven of the preparations of HA from the peatland Turochak, which resulted in the acceleration of microclonal propagation of plants in vitro.

BIOLOGICALLY ACTIVE SUBSTANCES OF THE HERB OF URTICA DIOICA

I P Pavlov Russian Medical Biological Herald ◽

10.17816/pavlovj2017130-41 ◽

2006 ◽

Vol 14 (1) ◽

pp. 25

Author(s):

V. Ya. Yatziuk ◽

G. A. Chaly ◽

O. V. Soshnikova

Keyword(s):

Total Content ◽

Urtica Dioica ◽

Water Soluble ◽

Biologically Active ◽

Raw Material ◽

Quantitative Content ◽

Phenol Composition ◽

First Time ◽

We have established the qualitative composition and the quantitative content of biologically active substances of the herb of Urtica dioica. Aminoacid composition, monosaccharide set of PSK and PV, the presence of 4 macro- and 18 microelements and their quantitative content have been studied for the first time. Investigating the phenol composition of the herb of Urtica dioica by HPLC has revealed in the raw material alongside with the previously described gallic, coffeic acids, rutine and quercetine the presence of hyperoside, luteolin-7-glycoside, 3,4-isoramnetin diglycoside, chlorogenic acid, aesculetin. We were the first to determine the total content of water-soluble polysaccharides, pectin substances, astringent substances, pigments (carotenoids and chlorophylls),vitamin K1, triterpene compounds and saponins of the triterpene type.

Phytochemical Analysis of Elaeagnus argentea Leaves

Drug development & registration ◽

10.33380/2305-2066-2021-10-3-95-99 ◽

2021 ◽

Vol 10 (3) ◽

pp. 95-99

Author(s):

N. A. Salnikova ◽

Yu. V. Shur ◽

A. A. Tsibizova

Keyword(s):

Ascorbic Acid ◽

Quantitative Determination ◽

Raw Materials ◽

Biologically Active ◽

Raw Material ◽

Phytochemical Analysis ◽

Promising Source ◽

Introduction. Currently, close attention in the field of pharmacy and medicine is directed to the search for new sources of biologically active substances of various origins, including vegetable. The woody plant Elaeagnus argentea is considered as a promising source. Despite its widespread use in the food industry, folk medicine as an anti-inflammatory, restorative, antimicrobial agent, the chemical composition of this plant has not been sufficiently studied.Aim. Qualitative and quantitative determination of the main groups of biologically active substances (BAS) in the raw material (leaves) of Elaeagnus argentea growing on the territory of the Astrakhan region for further development of a methodology for standardizing the raw material of this plant.Materials and methods. The leaves of Elaeagnus argentea were harvested in the spring in the eastern part of the delta of the Astrakhan region (Volga region). Drying of raw materials was carried out in natural conditions. In the study, unified methods were used: to determine the amount of flavonoids and saponins, the spectrophotometric method was used, ascorbic acid - the titrimetric method. For the analysis of flavonoids in the leaves of Elaeagnus argentea, an aqueous-alcoholic solution of 70 % concentration was used as an extractant. The quantitative content of flavonoids was determined in the obtained raw material extract in terms of luteolin-7-glucoside. The amount of saponins in the leaves of Elaeagnus argentea was determined in terms of oleanolic acid. 96 % ethanol was used as an extractant. The quantitative determination of ascorbic acid in the aqueous extract of crushed raw materials was carried out by titrimetry based on the ability to reduce 2,6-dichlorophenolindophenol.Results and discussion. In the pharmacognostic study of biologically active substances in the leaves of Elaeagnus argentea, the content of ascorbic acid was found to be at least 0.32 %, the amount of flavonoids in terms of luteolin-7-glucoside was at least 1.92 %; saponins - 2.38 %; which indicates the need for a more detailed study of the phytochemical composition of other morphological groups of the plant Elaeagnus argentea growing in the Astrakhan region.Conclusion. The data obtained during the study can be used to confirm the quality of raw materials (leaves) of Elaeagnus argentea. A more detailed study of leaves for the presence of other groups of biologically active substances will make it possible to use the obtained data for the development of regulatory documents for medicinal plant raw materials «Elaeagnus argentea leaves».

EXTRACTION OF FLAVONOIDS FROM KOENIGIA WEYRICHII USING DEEP EUTECTIC MIXTURE OF CHOLINE CHLORIDE + GLYCERINE

chemistry of plant raw material ◽

10.14258/jcprm.2021049530 ◽

2021 ◽

pp. 199-206

Author(s):

Nikita Sergeyevich Tsvetov ◽

Anna Viktorovna Korovkina ◽

Oksana Ivanovna Paukshta

Keyword(s):

High Efficiency ◽

Choline Chloride ◽

Eutectic Mixture ◽

Total Content ◽

Climatic Conditions ◽

Biologically Active ◽

The Arctic ◽

Promising Source ◽

Koenigia Weyrichii (F. Schmidt) T.M. Schust. et Reveal, which grows on the Kola Peninsula, contains a significant amount of flavonoids in the aerial parts of the plant, rapidly builds up biomass, and is resistant to various climatic conditions. This makes K. Weyrichii a promising source of biologically active substances (BAS) in the Arctic zone. A relatively new method for extracting biologically active substances from plants is the use of deep eutectic solvents (DES), for example, a mixture of choline chloride with glycerol, which has shown high efficiency in the extraction of flavonoids from other plants. Previously, DES was not used for the extraction of biologically active substances from K. Weyrichii. The aim of this work is to evaluate the efficiency of ultrasonic extraction using traditional solvents (water, ethanol) and DES choline chloride + glycerol and to optimize the extraction conditions. Comparison of the total content of polyphenols, flavonoids, antioxidative, and antiradical activity in various extracts is carried out. It was found that the most favorable conditions for extraction are: water content in DES – 17.5 wt%, temperature – 65 °C, and time – 3 hours. At the same time, under identical extraction conditions (temperature and time), more target substances can be extracted with 60% ethanol than with DES. However, DES can be seen as an alternative to using ethanol. The data obtained can be useful for the further development of innovative technologies of the extraction of biologically active substances from plant material. Also, K. Weyrichii may be considered as a source of biologically active substances in cosmetics and the food industry.

ANTIOXIDANT ACTIVITY OF THE EXTRACT OF CHICKWEED (STELLARIA MEDIA)

chemistry of plant raw material ◽

10.14258/jcprm.2018043749 ◽

2018 ◽

pp. 141-147 ◽

Cited By ~ 1

Author(s):

Баяна (Bayana) Анатольевна (Anatol'yevna) Баженова (Bazhenova) ◽

Галина (Galina) Батоевна (Batoyevna) Ендонова (Yendonova) ◽

Татьяна (Tat'yana) Петровна (Petrovna) Анцупова (Antsupova) ◽

Юлия (Yuliya) Юрьевна (Yur'yevna) Забалуева (Zabaluyeva) ◽

Александр (Aleksandr) Викторович (Viktorovich) Герасимов (Gerasimov)

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Pantothenic Acid ◽

Total Content ◽

Beta Carotene ◽

Biologically Active ◽

Raw Material ◽

Stellaria Media ◽

The paper presents new data on the chemical composition of the chickweed (Stellaria media), which grows in the Republic of Buryatia. Chickweed is a promising raw material because of its vitamins, flavonoids and trace elements, but the potential of this plant has not been studied enough. The aim of the work was studying the content of biologically active substances with antioxidant properties in the extract of chickweed, collected in the Zabaikal's region, and establish its antioxidant effect on lipid peroxidation. As a result of the studies, a high content of carbohydrates in leaves (3.04%), stems (3.41%) and roots (4.52%) of Stellaria media was established. The content of substances of protein nature was from 2.25% to 2.94%, mineral substances – from 1.42% to 1.74%. A higher content of flavonoids in the stems and leaves of the Stellaria media was found to be approximately twice as large as their content in the root system. The quantitatively and quantitatively content of organic acids (formic, malic, citric, acetic, succinic, oxalic, benzoic, fumaric), vitamins and provitamins (riboflavin, pantothenic acid, folic acid, ascorbic acid, tocopherol, beta-carotene) have been identified in the extract from Stellaria media. The total antioxidant activity of the extracts was established. The highest total content of antioxidants is in an aqueous extract of the leaves, which contributes to the inhibition of processes of animal fats due to the presence of biologically active substances with powerful antioxidant properties.