Morpho-biochemical evaluation of EMS regenerated mutants of Bergenia ciliata (Haw.) Sternb. under in vitro conditions

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Sabreena Rafi ◽  
Azra N. Kamili ◽  
B. A. Ganai ◽  
Mohammad Yaseen Mir ◽  
Javid A. Parray
Keyword(s):  
Adverse Effect ◽  
Seed Germination ◽  
Soluble Protein ◽  
Shoot Length ◽  
Root Number ◽  
Soluble Protein Content ◽  
Total Carbohydrate ◽  
Total Carbohydrate Content ◽  
Biochemical Evaluation

The present work has been carried out with the objective to evaluate the morpho-biochemical changes in the EMS generated mutants of Bergenia ciliata before transferring them to field conditions. In this regard, range of EMS doses from 0.1% to 0.9% have been given. The present doses have shown adverse effect on seed germination, root number and decreased soluble protein content of plant. However shoot length, root length, chlorophyll content and total carbohydrate content were improved at lower doses (0.3%) of EMS treatment and decreased thereafter.

scholarly journals IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Wheat Genotypes ◽  
Maximum Reduction ◽  
Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

Effects of auxins on soluble carbohydrates, starch and soluble protein content in Aechmea blanchetiana (Bromeliaceae) cultured in vitro

2010 ◽  
Vol 125 (3) ◽  
pp. 451-455 ◽  
Author(s):  
Edison Paulo Chu ◽  
Armando Reis Tavares ◽  
Shoey Kanashiro ◽  
Patricia Giampaoli ◽  
Erika Szeibel Yokota
Keyword(s):  
Protein Content ◽  
Soluble Protein ◽  
Soluble Carbohydrates ◽  
Soluble Protein Content

scholarly journals In vitro Callus Induction under NaCl Salt Stress and Subsequent Plant Regeneration in Oats (Avena sativa L.)

2020 ◽  
Vol 30 (2) ◽  
pp. 253-266
Author(s):  
AA Mamun ◽  
MM Islam ◽  
NEK Alam ◽  
S Noushin
Keyword(s):  
Salt Stress ◽  
Plant Regeneration ◽  
Avena Sativa ◽  
Callus Induction ◽  
Shoot Length ◽  
Root Number ◽  
Ex Vitro ◽  
Best Response ◽  
Mature Seeds

An effort was made to identify the in vitro responsiveness for callus induction in NaCl (salt stress) and subsequent plant regeneration of oats (Avena sativa L.). Callus induction was tested in different concentrations (0, 5, 10, 15 and 20 mg/l) of NaCl salt added in MS. The experiment was laid out in CRD considering 2,4-D growth regulator and NaCl salt as factors. The best response in callus induction was observed on MS containing 3 mg/l 2,4- D and 5 mg/l NaCl where the frequency of callus induction was 68.57%, callus weight was 0.36 g and callus diameter was 7.63 mm. During regeneration, the maximum frequency of regeneration (41.67%), regenerated shoots per callus (14.80), shoot length (12.14 cm), root number (10.90) and root length (3.76 cm) was observed from the callus derived from MS containing 3 mg/l 2,4-D and 5 mg/l NaCl. Plantlets that were regenerated from callus induced in MS with 3 mg/l 2,4-D and 5 mg/l NaCl showed higher rate of ex vitro survivability. Therefore, MS with 3 mg/l 2,4-D and 5 mg/l NaCl found better for in vitro callus induction and subsequent plant regeneration from mature seeds of oat. Plant Tissue Cult. & Biotech. 30(2): 253-266, 2020 (December)

scholarly journals Effect of Seed Orientation and Medium Strength on In vitro Germination of Pterocarpus marsupium Roxb.

2013 ◽  
Vol 5 (4) ◽  
pp. 476-479 ◽  
Author(s):  
Yogeshwar MISHRA ◽  
Rimi RAWAT ◽  
Brajesh NEMA ◽  
Fatima SHIRIN
Keyword(s):  
Shoot Length ◽  
Ms Medium ◽  
Root Number ◽  
Pterocarpus Marsupium ◽  
Shoot Number ◽  
Medium Strength ◽  
Half Strength ◽  
Varied Response ◽  
Strength Medium

A method was developed for optimization of In vitro germination of an economically important timber-yielding multipurpose tree, Pterocarpus marsupium Roxb. The seeds inoculated in different orientation on different strengths of MS medium without any hormones showed varied response to the seed positions. The seeds inoculated on half strength medium in horizontal position recorded to produce a maximum germination (78.23%), shoot number (0.86) and root number (7.99). However, a maximum of shoot length of 3.67 cm was recorded in the quarter strength medium in the seeds inoculated in vertical down position, which was significantly higher than other media strength and positions. Our results indicate that the seed orientation including medium strength have tremendous effect on germination and seeds inoculated horizontally on half strength MS medium can be utilized to enhance In vitro seed germination of Pterocarpus marsupium.

scholarly journals In vitro seed germination and Growth of Three Varieties of Black Gram after Ultraviolet-B Radiation

2016 ◽  
Vol 4 (1) ◽  
pp. 117-129 ◽  
Author(s):  
K Rajendiran ◽  
K Thiruvarasan ◽  
R Vijayalakshmi
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Plant Biomass ◽  
Shoot Length ◽  
Ultraviolet B ◽  
Black Gram ◽  
Root And Shoot Length ◽  
Dry And Wet Conditions ◽  
Germination And Growth

In vitro seed germination and growth of seedlings was tried with three varieties of black gram (Vigna mungo (L.) Hepper) viz. Vamban-3, Nirmal-7 and T-9 after ultraviolet-B irradiation (UV-B = 2 hours once with 1 hour recovery time @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1). Unstressed and UV-B stressed VAMBAN-3 and T-9 seeds both in dry and wet conditions responded to in vitro germination. Unstressed NIRMAL-7 failed to germinate under in vitro culture. UV-B stressed NIRMAL-7 responded to in vitro culture. UV-B irradiation enhanced seedling height at both dry and wet conditions in VAMBAN-3 followed by NIRMAL-7 compared with controls. Root and shoot length of UV-B stressed VAMBAN-3 and NIRMAL-7 performed five to six times better than control. Root and shoot length of T-9 was reduced (2.61 to 8.69 %) below control after UV-B exposure. UV-B stressed VAMBAN-3 under dry and wet exposure accumulated three to six times more plant biomass over controls. UV-B stressed NIRMAL-7 and T-9 dry seeds accumulated less plant biomass by 70.86 % and 12.39 % respectively than their controls. NIRMAL-7 and T-9 under dry UV-B exposure produced two times more leaves than control.Int J Appl Sci Biotechnol, Vol 4(1): 117-129

scholarly journals Shoot Proliferation and Growth of Gardenia (Gardenia jasminoides Ellis) in Response to pH and Sugar Concentrations in Vitro

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 461B-461
Author(s):  
Karim H. Al-Juboory ◽  
L.K. Al-Amiry ◽  
M.M Kushad
Keyword(s):  
Shoot Proliferation ◽  
Dry Weight ◽  
Shoot Length ◽  
Stem Cuttings ◽  
Root Number ◽  
Fresh Weight ◽  
Gardenia Jasminoides ◽  
Shoot Number ◽  
Effects Of Ph

Stem cuttings of greenhouse-grown gardenia (Gardenia jasminoides Ellis) were surface-sterilized and planted on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L (BA) and 0.5 mg/L (NAA). This study examines the effects of pH and various sugars on the growth and proliferation of in vitro-cultured gardenia. The highest average shoot number and shoot length were obtained at pH 4.0 to 5.0. In determining the effect of sugars on shoot proliferation, our findings indicated that sucrose and glucose at 30 and 40 g/L, respectively, produced a higher shoot number when compared to fructose and xylose. In addition, sucrose and glucose produced the highest root number, root length, and rooting percentage, while fructose and xylose had no effect on rooting. Sucrose and fructose produced more calli in comparison to other sugars. Xylose at 40 g/L produced the highest dry weight (18.5%), while xylose at 10 g/L produced the highest fresh weight (94.4%).

scholarly journals Physio-biochemical Responses of In Vitro Cooking Banana Musa paradisiaca cv Lang towards Pseudo Induced Drought Stress by Polyethylene Glycol (PEG)

2021 ◽  
Vol 17 (6) ◽  
pp. 805-817
Author(s):  
Sharifah Zafierah Syed Badrulzaman ◽  
Nurhana Nadia Ramlan ◽  
Munirah Adibah Kamarul Zaman ◽  
Azzreena Mohamad Azzeme
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Soluble Protein ◽  
Proline Content ◽  
Total Soluble Protein ◽  
Soluble Protein Content ◽  
Musa Paradisiaca ◽  
Cooking Banana ◽  
Total Soluble Protein Content

Musa paradisiaca cv Lang belongs to cooking banana group, and it has high potential to be used in banana chips production. Like other cultivars, M. paradisiaca cv Lang is susceptible towards water shortage, therefore affecting banana growth and productivity. In this study, to mimic the drought condition, pseudo-drought stress was given to in vitro Lang banana seedlings by adding polyethylene glycol (PEG). Overall, decrement of roots length and chlorophyll (Chl) content was displayed by the seedlings exposed to 1%, 2%, 3%, 4%, and 5% (w/v) of PEG after three weeks of exposure. The proline content, total soluble protein content, and antioxidant capacity in leaf and roots, however, countered differently towards different levels of drought. Proline content showed the highest in leaf of 2% (w/v) PEG-treated seedling (12.66±0.38 µmoles/g) while the total soluble protein content showed the highest in roots of 5% (w/v) of PEG-treated seedling (30.65±1.07 mg/g FW). Antioxidant capacity of stressed seedlings revealed the catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) activities were the highest in the leaf of 1% (w/v) (10.69±5.06 µmol/min/mg), 4% (w/v), (0.079±0.03 µmol/min/mg), and 5% (w/v) (9.11±8.47 µmol/min/mg) of PEG- treated seedlings, respectively. Meanwhile, the highest CAT, POD, and APX activities in the roots were determined in 3% (w/v) (0.49±0.04 µmol/min/mg), 2% (w/v) (0.03±0.02 µmol/min/mg), and 3% (w/v) (16.69±0.5 µmol/min/mg) of PEG-treated seedlings, respectively. These data show that PEG can be a priming agent to induce defense system at seedling stage of banana, which could enhance their survivability during ex vitro acclimatization.

scholarly journals The Stimulating Effects of Imazalil and Carbendazim Fungicides on in Vitro Propagation and ex Situ conservation of the Medicinal Balkan Range-Restricted Sideritis Raeseri Boiss & Heldr. Subsp. Raeseri

2019 ◽  
Vol 10 ◽  
pp. 1752-1763 ◽  
Author(s):  
Virginia Sarropoulou ◽  
Eleni Maloupa
Keyword(s):  
Large Scale ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Ex Situ ◽  
Root Number ◽  
Short Period ◽  
Shoot Number

Sideritis raeseri Boiss & Heldr. subsp. raeseri, known in Greece as Mountain tea of Parnassus or Velouchi is a range restricted medicinal plant of the Balkan peninsula. Conventional propagation methods did not allow the mass production of plant material in a short period of time due to both low seed germination rate and rooting of cuttings. Therefore, the aim of this study was to establish a reliable, reproducible and efficient regeneration protocol for mass and large-scale micropropagation, germplasm and ex situ conservation of S. raeseri Boiss & Heldr. using Imazalil and Carbendazim fungicides. After 9 weeks, 2.5-10 mg/l Imazalil stimulated root length by 1 cm but diminished root number and rooting percentage. Optimum shoot number (5.58), shoot length (24.91 mm), shoot multiplication (100%), root number (20.63) and rooting (66.67%) were recorded with 0.5 mg/l kinetin (KN) + 0.05 mg/l ?-napthaleneacetic acid (NAA) (Imazalil-free). After 4 ½ weeks, 1 mg/l Carbendazim + 0.5 mg/l 6-benzyladenine (BA) yielded 5.77 shoots/explant, 16.1 mm shoot length and 100% shoot multiplication. However, Carbendazim did not result in rooting. The ex vitro survival percentage of rooted shoot-tip explants derived from Imazalil experiment was 95%. Carbendazim proved useful in shoot proliferarion and Imazalil in root elongation of S. raeseri Boiss & Heldr. subsp. raeseri micropropagation system.  

scholarly journals Germinação in vitro e aclimatização de Hamatocactus setispinus

2015 ◽  
Vol 21 (2) ◽  
pp. 193
Author(s):  
Priscilla Brites Xavier ◽  
Janie Mendes Jasmim
Keyword(s):  
Root Length ◽  
Dry Matter ◽  
Seedling Survival ◽  
Basal Medium ◽  
Shoot Length ◽  
In Vitro Germination ◽  
Root Number ◽  
Fresh Matter ◽  
Dry Matter Weight

Seed propagation preserves the population genetic variability and helps selecting desirable features. This study evaluated the in vitro germination of Hamatocactus setispinus in six different culture media, 1- MS basal medium full strength; 2- half-strength MS basal medium; 3- 1.0 g L-1 of Peter’s CalMag® 15-05-15 formulation; 4- 0.5 g L-1 of Peter’s CalMag® 15-05-15 formulation; 5- MS basal medium supplemented with 10% coconut water and; 6- water and agar, with and without activated charcoal, and the speed of germination index, the mean germination time and the germination rate, root length, shoot length and the number of roots were evaluate. The seedlings with superior development obtained from in vitro germination were acclimatized in two substrates: Biomix® Floreira; Biomix® Floreira + sand. Seedling survival, shoot length, shoot diameter, root length, root number, shoot fresh matter weight, root fresh matter weight, shoot dry matter weight and root dry matter weight were evaluated. Peter’s 1.0 g L-1 medium without activated charcoal led to the best results for root length (11.36 mm) and root number (3.84). There was 100% of seedling survival. Acclimatization substrates did not differ among themselves and, therefore, they did not affect seedling growth.

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