scholarly journals Study of gentamicin deposition in cellulose with albumin

2019 ◽  
Vol 49 (5) ◽  
pp. 61-66
Author(s):  
T. E. Mironova ◽  
V. N. Afonyushkin ◽  
N. A. Sigareva ◽  
I. N. Tromenshleger ◽  
A. V. Kharchenko
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Bacterial Cellulose ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Test Strain ◽  
Gluconacetobacter Hansenii ◽  
Antibacterial Drugs ◽  
Protein Gel ◽  
Partial Denaturation

Methods of binding antibacterial drugs to the surface of cellulose without the use of oxidizing agents to prevent the occurrence of wound infections have been studied. The immobilization of gentamicin in the complex of partially denatured albumin in the composition with bacterial cellulose has been analyzed. The study was carried out on samples of cellulose synthesized by Gluconacetobacter hansenii. Albumin served as a binding agent, which was used to impregnate cellulose samples, which were then denatured. Using PCR amplifi cation CFX (BioRad), the optimal denaturation temperature was selected. The effectiveness of the immobilization of albumin in the thickness of the cellulose was assessed by staining it with the luminescent dye SYPRO® Ruby Protein Gel Stain, followed by transilluminator detection. Bacterial cellulose impregnated with undenatured albumin was used as a control. Albumin immobilization in bacterial cellulose was observed at temperatures of 65– 95 °C. The antibacterial activity of the complex “cellulose + albumin + gentamicin” was evaluated using a test strain of bacteria Staphylococcus aureus ATCC 25923. The growth inhibition of the test strain of bacteria was observed in all tests with bacterial cellulose in combination with partially denatured albumin and gentamicin. In control samples, in which gentamicin was not immobilized as part of partially denatured albumin, growth inhibition zones of Staphylococcus aureus ATCC 25923 were not noted. It was concluded that by partial denaturation of albumin it is possible to delay antibacterial drugs in the thickness of bacterial cellulose for their further release. A new version of the material suitable for the production of implants and bandages based on bacterial cellulose gel with antibacterial properties is proposed. Dressings based on a composite of bacterial cellulose, albumin and gentamicin are most relevant for the treatment of burns. The presence of gentamicin in their composition is also relevant for the prevention of bacterial infections.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

scholarly journals Au–ZnO Conjugated Black Phosphorus as a Near-Infrared Light-Triggering and Recurrence-Suppressing Nanoantibiotic Platform against Staphylococcus aureus

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Atanu Naskar ◽  
Sohee Lee ◽  
Kwang-sun Kim
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Au Nanoparticles ◽  
Synthesis Method ◽  
Antibacterial Properties ◽  
Black Phosphorus ◽  
Infrared Light ◽  
Resistant Bacteria ◽  
Near Infrared Light

Antibiotic therapy is the gold standard for bacterial infections treatment. However, the rapid increase in multidrug-resistant (MDR) bacterial infections and its recent use for secondary bacterial infections in many COVID-19 patients has considerably weakened its treatment efficacy. These shortcomings motivated researchers to develop new antibacterial materials, such as nanoparticle-based antibacterial platform with the ability to increase the chances of killing MDR strains and prevent their drug resistance. Herein, we report a new black phosphorus (BP)-based non-damaging near-infrared light-responsive platform conjugated with ZnO and Au nanoparticles as a synergistic antibacterial agent against Staphylococcus aureus species. First, BP nanosheets containing Au nanoparticles were assembled in situ with the ZnO nanoparticles prepared by a low-temperature solution synthesis method. Subsequently, the antibacterial activities of the resulting Au–ZnO–BP nanocomposite against the non-resistant, methicillin-resistant, and erythromycin-resistant S. aureus species were determined, after its photothermal efficacy was assessed. The synthesized nanocomposite exhibited excellent anti-S. aureus activity and good photothermal characteristics. The non-resistant S. aureus species did not produce drug-resistant bacteria after the treatment of multiple consecutive passages under the pressure of the proposed nanoantibiotic, but rapidly developed resistance to erythromycin. This work clearly demonstrates the excellent photothermal antibacterial properties of Au–ZnO–BP nanocomposite against the MDR S. aureus species.

Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis

2017 ◽  
Vol 18 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Roza Haghgoo ◽  
Motahareh Ahmadvand ◽  
Mohammad Nyakan ◽  
Mojtaba Jafari
Keyword(s):  
Zinc Oxide ◽  
Growth Inhibition ◽  
Enterococcus Faecalis ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zinc Oxide Eugenol ◽  
Nanosilver Particles ◽  
Inhibition Zones

ABSTRACT Aim This study aimed to assess the antimicrobial efficacy of 0, 0.5, 2, and 5 wt% nanosilver in conjunction with zinc oxide eugenol (ZOE) against Enterococcus faecalis. Materials and methods Nanosilver in 0.5, 2, and 5 wt% concentrations was added to ZOE and the antibacterial activity of the mixtures on E. faecalis was assessed using disk diffusion method, and the results were reported as the diameter of the growth inhibition zone. Results The diameters of the growth inhibition zones around 0, 0.5, 2, and 5 wt% concentrations of nanosilver particles were not significantly different at 24 and 48 hours and 1 week; however, the difference with the azithromycin disk was significant. Conclusion Considering the lack of a significant increase in the diameter of the growth inhibition zones around 0, 0.5, 2, and 5 wt% ZOE containing nanosilver, it appears that addition of nanosilver up to 5 wt% cannot improve the antibacterial properties of ZOE sealer against E. faecalis. Clinical significance Microorganisms present in the root canal system of primary teeth are mainly responsible for endodontic infections. Enterococcus faecalis is the most important cause of endodontic failure. Application of sealers that decrease the adhesion and colonization of bacteria, as well as susceptibility to bacterial infections can greatly help in this regard. Using these sealers in conjunction with antibacterial agents, such as nanosilver particles may yield higher antibacterial efficacy. How to cite this article Haghgoo R, Ahmadvand M, Nyakan M, Jafari M. Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis. J Contemp Dent Pract 2017;18(3):177-181.

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

scholarly journals A new, reliable, and high-throughput strategy to screen bacteria for antagonistic activity against Staphylococcus aureus

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Soyoun Park ◽  
Adam Classen ◽  
Hanny Maeva Gohou ◽  
Roberto Maldonado ◽  
Emily Kretschmann ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Bacterial Infections ◽  
Bovine Milk ◽  
Quorum Quenching ◽  
Culture Conditions ◽  
Antagonistic Activity ◽  
Inhibiting Activity ◽  
Reporter Protein ◽  
Growth Inhibiting

Abstract Background Antibiotic-resistant Staphylococcus aureus clones have emerged globally over the last few decades. Probiotics have been actively studied as an alternative to antibiotics to prevent and treat S. aureus infections, but identifying new probiotic bacteria, that have antagonistic activity against S. aureus, is difficult since traditional screening strategies are time-consuming and expensive. Here, we describe a new plasmid-based method which uses highly stable plasmids to screen bacteria with antagonistic activity against S. aureus. Results We have created two recombinant plasmids (pQS1 and pQS3) which carry either gfpbk or mCherry under the control of a S. aureus quorum-sensing (QS) promoter (agrP3). Using this recombinant plasmid pair, we tested 81 bacteria isolated from Holstein dairy milk to identify bacteria that had growth-inhibiting activity against S. aureus and suggest potential explanations for the growth inhibition. The stability test illustrated that pQS1 and pQS3 remained highly stable for at least 24 h in batch culture conditions without selection pressure from antibiotics. This allowed co-culturing of S. aureus with other bacteria. Using the newly developed pQS plasmids, we found commensal bacteria, isolated from raw bovine milk, which had growth-inhibiting activity (n = 13) and quorum-quenching (QQ) activity (n = 13) towards both S. aureus Sa25 (CC97) and Sa27 (CC151). The pQS-based method is efficient and effective for simultaneously screening growth-inhibiting and QQ bacteria against S. aureus on agar media. Conclusions It was shown that growth-inhibiting and QQ activity toward pQS plasmid transformants of S. aureus can be simultaneously monitored by observing the zone of growth inhibition and reporter protein inhibition on agar plates. Newly identified antagonistic bacteria and their functional biomolecules are promising candidates for future development of probiotic drugs and prophylactics/therapeutics for bacterial infections including S. aureus. Furthermore, this new approach can be a useful method to find bacteria that can be used to prevent and treat S. aureus infections in both humans and animals.

scholarly journals HONEY AS A THERAPEUTIC AGENT, AN INSIGHT INTO ITS ANTIMICROBIAL PROPERTIES.

2020 ◽  
Author(s):  
FOSU ASARE
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Therapeutic Agent ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Close Association ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Medical Practitioners ◽  
Ancient Times ◽  
Insight Into

Traditionally, honey has been used as both food and medicine since ancient times worldwide. Due to its nutritious content and sweet flavor, honey has been in great demand worldwide with China being the leading producer. People apply honey on the wound without having any knowledge of its antibacterial properties. Honey has proven to contain some antibacterial properties such as hydrogen peroxide, acidity, low moisture content, pH, etc. Bacteria are ubiquitous and their life has a close association with humans in their diet, food processing, etc. Some bacteria eg Staphylococcus aureus, consist of the normal flora of humans making them partially harmless but some can be harmful and in turn cause infections. Clinically, antibiotics such as tetracycline, penicillin, amoxicillin lincomycin, etc. have been used in the treatment of bacterial infections but some bacteria eg Methicillin Resistant Staphylococcus aureus have shown resistance to some antibiotics. Medical practitioners have opted for the use of honey in treating bacterial infections due to its antibacterial properties. Researches have proven that honey can kill or inhibit almost every bacteria and is very effective in wound treatment as compared to antibiotics. In light of this most medical practitioners prefer the use of honey in treating bacterial infections.

Fluoroquinolone-3-carboxamide Amino Acid Conjugates: Synthesis, Antibacterial Properties And Molecular Modeling Studies

2020 ◽  
Vol 17 (1) ◽  
pp. 71-84
Author(s):  
Riham M. Bokhtia ◽  
Siva S. Panda ◽  
Adel S. Girgis ◽  
Hitesh H. Honkanadavar ◽  
Tarek S. Ibrahim ◽  
...  
Keyword(s):  
Experimental Data ◽  
Antibacterial Activity ◽  
Amino Acid ◽  
Bacterial Infections ◽  
Antibacterial Agents ◽  
Antibacterial Properties ◽  
Computational Studies ◽  
Protecting Group ◽  
Amino Acid Conjugates ◽  
Molecular Modeling Studies

Background: Bacterial infections are considered as one of the major global health threats, so it is very essential to design and develop new antibacterial agents to overcome the drawbacks of existing antibacterial agents. Method: The aim of this work is to synthesize a series of new fluoroquinolone-3-carboxamide amino acid conjugates by molecular hybridization. We utilized benzotriazole chemistry to synthesize the desired hybrid conjugates. Result: All the conjugates were synthesized in good yields, characterized, evaluated for their antibacterial activity. The compounds were screened for their antibacterial activity using methods adapted from the Clinical and Laboratory Standards Institute. Synthesized conjugates were tested for activity against medically relevant pathogens; Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27856) Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 19433). Conclusion: The observed antibacterial experimental data indicates the selectivity of our synthesized conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data were supported by computational studies.

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