Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis

2017 ◽  
Vol 18 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Roza Haghgoo ◽  
Motahareh Ahmadvand ◽  
Mohammad Nyakan ◽  
Mojtaba Jafari
Keyword(s):  
Zinc Oxide ◽  
Growth Inhibition ◽  
Enterococcus Faecalis ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zinc Oxide Eugenol ◽  
Nanosilver Particles ◽  
Inhibition Zones

ABSTRACT Aim This study aimed to assess the antimicrobial efficacy of 0, 0.5, 2, and 5 wt% nanosilver in conjunction with zinc oxide eugenol (ZOE) against Enterococcus faecalis. Materials and methods Nanosilver in 0.5, 2, and 5 wt% concentrations was added to ZOE and the antibacterial activity of the mixtures on E. faecalis was assessed using disk diffusion method, and the results were reported as the diameter of the growth inhibition zone. Results The diameters of the growth inhibition zones around 0, 0.5, 2, and 5 wt% concentrations of nanosilver particles were not significantly different at 24 and 48 hours and 1 week; however, the difference with the azithromycin disk was significant. Conclusion Considering the lack of a significant increase in the diameter of the growth inhibition zones around 0, 0.5, 2, and 5 wt% ZOE containing nanosilver, it appears that addition of nanosilver up to 5 wt% cannot improve the antibacterial properties of ZOE sealer against E. faecalis. Clinical significance Microorganisms present in the root canal system of primary teeth are mainly responsible for endodontic infections. Enterococcus faecalis is the most important cause of endodontic failure. Application of sealers that decrease the adhesion and colonization of bacteria, as well as susceptibility to bacterial infections can greatly help in this regard. Using these sealers in conjunction with antibacterial agents, such as nanosilver particles may yield higher antibacterial efficacy. How to cite this article Haghgoo R, Ahmadvand M, Nyakan M, Jafari M. Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis. J Contemp Dent Pract 2017;18(3):177-181.

scholarly journals Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Tadeusz Bold ◽  
Grzegorz Ziółkowski ◽  
Marcin Pierchała ◽  
Aleksandra Królikowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Bovine Thrombin ◽  
Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals EFFECTIVITY ANTIBACTERIAL ZINC OXIDE EUGENOL WITH ZINC OXIDE PROPOLIS FOR ENDODONTIC TREATMENT IN PRIMARY TEETH

2019 ◽  
Vol 6 (2) ◽  
pp. 113
Author(s):  
Erwid Fatchur Rahman ◽  
Sandy Christiono
Keyword(s):  
Zinc Oxide ◽  
Enterococcus Faecalis ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Blood Agar Plate ◽  
Control Group ◽  
Control Group Design ◽  
Normality Test ◽  
Zinc Oxide Eugenol ◽  
Post Test

Background: Enterococcus faecalis is generally found on the failure of root canal treatments. Zinc oxide propolis is believed to have an antibacterial effect on that bacteria. This research aimed to compare bacteriostatic effect of zinc oxide eugenol (ZOE) and zinc oxide propolis (ZOP) as the sealer materials of root canal.Method: This was an experimental research with post-test only control group design with two different groups (ZOE and ZOP). Culture of Enterococcus faecalis bacteria was smeared on Blood Agar Plate media with six times replication per group and kept inside incubator for 24 hours. The result was obtained from the inhibition zone formed around the pasta.Result: The average result of ZOE and ZOP was 27.7 mm and 13.45 mm respectively. Normality test using Shapiro-Wilks showed that data was normal (p>0.05). Then, the data was analysed using Independent Samples T-test. The result showed that there was different inhibition zone between ZOE group and ZOP group (p<0.05).Conclusion: Based on the result, it can be concluded that ZOP has lower antibacterial effectiveness of the Enterococcus faecalis than ZOE.

scholarly journals Antimicrobial efficacy of herbal extracts combined with zinc oxide eugenol as an obturating material in primary teeth – An in vitro study

2019 ◽  
Vol 8 (4) ◽  
pp. 244
Author(s):  
SaiSankar J. Avula ◽  
Senapathi Navaneet ◽  
Sridhar Muktineni ◽  
Pranitha Kakarla ◽  
HarishC Kommineni ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Primary Teeth ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Herbal Extracts ◽  
Zinc Oxide Eugenol

scholarly journals Synthesis of Chitosan/Zinc Oxide Nanoparticles Stabilized by Chitosan via Microwave Heating

2019 ◽  
Vol 14 (2) ◽  
pp. 450 ◽  
Author(s):  
Nurul Amira Ahmad Yusof ◽  
Norashikin Mat Zain ◽  
Norlin Pauzi
Keyword(s):  
Zinc Oxide ◽  
Microwave Heating ◽  
Zno Nanoparticles ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Mean Value ◽  
Average Crystalline Size ◽  
Xrd Patterns

Nowadays, zinc oxide (ZnO) has attracted attention in research and development because of its remarkable antibacterial properties. Chitosan/ZnO nanoparticles were successfully synthesized via microwave heating. The objectives of this work were to investigate the effect of stabilizer, power heating and time heating on size of chitosan/ZnO nanoparticles and to determine antibacterial activity against pathogenic bacteria, where chitosan was used as a stabilizing agent. Chitosan/ZnO nanoparticles were analyzed  by Fourier Transform Infra Red (FTIR), X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Zetasizer instrument. The power heating and time heating were varied from 400 to 800 Watt and 4 to 8 minutes, respectively. The presence of chitosan has role on preventing the nanoparticles from agglomeration by producing a milky solution of chitosan/ZnO nanoparticles without any suspensions. The increase of power  and time heating improved the size of nanoparticles. The peak in FTIR spectrum at around 427 cm-1 was confirmed the existence of the ZnO phase. XRD patterns showed that the chitosan/ZnO nanoparticles materials were pure phase with average crystalline size is 130 nm. FESEM revealed that chitosan/ZnO nanoparticles were uniformly distributed with the mean value of size is 70 nm and spherical shaped. Further impact of power and time heating on the size of the chitosan/ZnO nanoparticles can be shown by a nanoparticles size distribution with the average of 30 to 90 nm. The results showed that chitosan/ZnO nanoparticles have displayed an antibacterial inhibition zone against Gram-positive S. aureus and Gram-negative E. coli which 16.0 and 13.3 mm, respectively. Chitosan/ZnO nanoparticles were synthesized in this work presented have potential application to prevent bacterial infections. Copyright © 2019 BCREC Group. All rights reserved 

scholarly journals Antimicrobial Efficacy of Few Commercially Available Herbal and Non Herbal Tooth Pastes Against Clinically Isolated Human Cariogenic Pathogens

2015 ◽  
Vol 5 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Amitava Bora ◽  
Sharmista Sen ◽  
Abhirup Goswami ◽  
Gautam Kumar Kundu ◽  
Biswajit Ghosh
Keyword(s):  
Antimicrobial Activity ◽  
Laboratory Method ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Occlusal Caries ◽  
Molar Teeth ◽  
Inhibition Zones ◽  
Research And Education ◽  
Herbal Components ◽  
Agar Well Diffusion Method

Objective: The aim of this study was to compare the antimicrobial efficacy of ten commercially available herbal and non herbal toothpastes. Methods: Ten toothpaste brands (A-J) were collected. A, C, D, E contained Herbal components only, toothpastes B, F, G contained herbal components and fluoride as main anticariogenic agent, toothpastes H and I contained fluoride and triclosan combination and toothpaste J contained fluoride only as main anticariogenic component. Dental caries samples were collected from 6-14 years old children having occlusal caries in permanent fully erupted molar teeth involving enamel and dentin but caries not involving dental pulp and Streptococcus mutans bacteria isolated by laboratory method. The antimicrobial activity was assessed by measuring the inhibition zones by agar well diffusion method. Results: Fluoridated toothpaste with triclosan had maximum antimicrobial activity at all concentrations when compared to Herbal toothpaste. Conclusion: Toothpastes containing fluoride and triclosan are most effective but herbal tooth pastes have the prospect of becoming safer and effective altenative and provide an ideal home care anticariogenic regimen. DOI: http://dx.doi.org/10.3329/bjdre.v5i1.22454 Bangladesh Journal of Dental Research and Education Vol.5(1) 2015: 14-18

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans

2017 ◽  
Vol 16 (01) ◽  
pp. 66-69
Author(s):  
Hena Rahman ◽  
Ramesh Chandra ◽  
Divya Chowdhary ◽  
Shailja Singh ◽  
Supratim Tripathi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Zinc Oxide Eugenol

scholarly journals Chemical Indices and Antibacterial Properties of Some Essential Oils of Apiaceae and Lauraceae Spices in Southwest of Algeria

2019 ◽  
Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Antibacterial Properties ◽  
Independent Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cinnamon Essential Oil ◽  
The One

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.

scholarly journals Gut Extracts of Rhynchophorus ferrugineus Larvae Olivier Affecting Bacterial Dental Caries

2021 ◽  
Author(s):  
G.H. Sewify ◽  
H.M. Hamada ◽  
Z.A. Mustafa ◽  
Hani A. Alhadrami
Keyword(s):  
Dental Caries ◽  
Morphological Changes ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Rhynchophorus Ferrugineus ◽  
Electron Microscopic ◽  
Inhibition Zones ◽  
The Mean ◽  
Klebsiella Spp

In vitro study was conducted to explore antibacterial properties of the larval gut extracts of Rhynchophorus ferrugineus (Red Palm Weevil) Oliver. Larval gut extracts were tested against salivary bacteria causing dental carries using the agar well diffusion method. The gut extracts significantly affected the growth of both Klebsiella spp. and Streptococcus viridans. The two bacterial species revealed significant differences in their sensitivity to the extract. The extract efficacy depended upon the concentration and time of exposure. When using 100%concentration of the extract, the mean of inhibition zones for S. viridans and Klebsiella spp. at 24 h after treatment were 1.61 mm and 2.50 mm, respectively. At 48 h post-treatment, the mean of inhibition zones for S. viridans and Klebsiella spp. were 1.96 mm and 2.66 mm. After 72 hours, the means zones were 2.28 mm and 2.91 mm, respectively. Electron microscopic examinations showed morphological changes of the outer membrane of bacteria with a noticeable damage as a result of exposure to the gut extract. The results suggest potential use of these extracts against dental caries bacteria.

Sign in / Sign up

Export Citation Format

Share Document