scholarly journals Serological monitoring of cattle mycoplasmosis

2020 ◽  
Vol 49 (6) ◽  
pp. 88-95
Author(s):  
M. A. Leonova ◽  
I. S. Onishchenko ◽  
N. Yu. Balybina ◽  
I. N. Pen’kova
Keyword(s):  
Enzyme Immunoassay ◽  
Carrier Phase ◽  
Active Form ◽  
Clinical Manifestations ◽  
Growth Dynamics ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Novosibirsk Region ◽  
Intensive Use ◽  
Chronic Course

The results of studying the immune response with persistence of the microorganism of the genus Mycoplasma in the body of cows are presented. The study (2019) was conducted in dairy farms in five districts of Novosibirsk region. Identification of individual specific antibodies of class G to microorganisms of the genus Mycoplasma was carried out in 186 samples of blood serum of cattle. The study was carried out by enzyme immunoassay with the MycoplasmaIgG antibodies ELISA VET kit. In the majority of the studied farms, a prolonged persistence of the microorganism of the genus Mycoplasma was noted. It was found that mycoplasma, having weak immunogenicity, mainly causes a chronic course of the disease. In an enzyme-linked immunoassay, this phenomenon was recorded in dubious reactions in 60.8% of animals. In some farms during the study, a period of reactivation of the disease was noted, which appeared in the transition of the disease from the carrier phase to the active form with clinical manifestations. In the enzyme immunoassay, 7.5% of the animals reacted positively. It was noted that in farms with positively reacting animals, the probability of isolation and spread of the pathogen from sick animals is high. No reaction to the presence of class G antibodies to microorganisms of the genus Mycoplasma was detected in 31.7% of the animals studied. In most farms, the growth dynamics of animals with dubious response was noted to depend on their physiological period. The connection of the duration of cow lactation with the dubious antibody response in an enzyme-linked immunosorbent assay was established. The possibility of connecting this phenomenon with highly intensive use of productive animals, which leads to an increase in stress levels and a decrease in homeostasis and immunity, is shown.

scholarly journals Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

2002 ◽  
Vol 60 (2B) ◽  
pp. 400-405 ◽  
Author(s):  
Newton Satoru Odashima ◽  
Osvaldo Massaiti Takayanagui ◽  
José Fernando de Castro Figueiredo
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Neurological Diseases ◽  
Active Form ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inactive Form ◽  
Cysticercus Cellulosae ◽  
Inflammation Signs

The objective of this study was to analyze different immunoglobulins classes (IgG, IgM, IgE and IgA) against Cysticercus cellulosae in the cerebrospinal fluid (CSF), through enzyme linked immunosorbent assay (ELISA), correlating them to clinical and tomographic profiles in patients with neurocysticercosis (NCC). Eighty-five specimens of CSF were obtained from 43 cases with NCC (26 with the active form and 17 with the inactive form) and from 42 patients with other neurological diseases. The inactive form of NCC presented a profile in CSF similar to the group without NCC. The active form of NCC presented elevation of specific immunoglobulins (IgG, IgM, IgE, and IgA) in decreasing order, with the highest values being detected among the cases with intraventricular cysts, or with inflammation signs in CSF or in those with multiple clinical manifestations. The highest sensitivity and specificity were obtained with ELISA-IgG (88.5% and 93.2%, respectively). This study confirmed the importance of ELISA in the immunologic diagnosis of NCC.

scholarly journals THE ROLE OF ENDOGENOUS NEUROPEPTIDES IN THE PATHOGENESIS AND CLINICAL MANIFESTATIONS OF ROSACEA-TIDES IN WOMEN

2021 ◽  
Vol 1 (4) ◽  
Author(s):  
Anas Sarayreh Atalla Salem ◽  
Larisa Kuts
Keyword(s):  
Substance P ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
The Body ◽  
Biologically Active ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Complex Nature ◽  
Common Skin ◽  
Regulatory Effects

Rosacea is a common skin disease with a chronic recurrent course. Numerous factors of etiology and pathogenesis of this dermatosis, which may influence the appearance of its clinical signs, have not been elucidated. The aim of the work is to determine the content of endogenous neuropeptides (substance P, leu-enkephalin) in the blood, to find out their role in the development of clinical manifestations of rosacea in women. Materials and methods - 47 women with rosacea were clinically and laboratory examined in whom the levels of substance P and leu-enkephalin in the blood were determined by enzyme-linked immunosorbent assay. Results. Quantitative assessment of the degree of manifestations of clinical signs of local redness of the skin and subsequent symptoms of rosacea (pink acne) in women indicates a complex nature of changes in the skin and reactions of the nervous and endocrine systems. The revealed imbalance in the blood of patients with endogenous neuropeptides with different mechanisms of effector and regulatory action (increase - substance P, decrease - leu-enkephalin) indicates a violation of the mutual compensatory relations of these peptide signaling molecules in different functional systems of the body, and the following symptoms of the disease, which differed in women of reproductive and older age.Conclusions. Imbalance of levels of endogenous neuropeptides with opposite effector and regulatory effects (substance P, leu-enkephalin) may be one of the key pathogenetic factors of the appearance and development of clinical manifestations of rosacea in women given the polypotent mechanisms of action of these biologically active compounds as neurotransmitters and antinociceptive mediators, regulators of emotional and behavioral reactions, pro- and anti-inflammatory factors, coordinators of the hypothalamic-pituitary-gonadal system. Misdiagnosis of such manifestations of the disease as rosacea-tides with «menopausal», «migraine-like» and other types of tides in women dictate the need for a comprehensive examination of such patients.Кey words: rosacea, substance P, ley-enkephalin, women’s.

Clinical, morphological and biochemical signs of polymycotoxicosis in highly productive cows

2020 ◽  
pp. 14-17
Author(s):  
Irina A. Shkuratova ◽  
◽  
Lyudmila I. Drozdova ◽  
Aleksander I. Belousov ◽  
Keyword(s):  
Metabolic Disorders ◽  
Hepatorenal Syndrome ◽  
Morphological Changes ◽  
Clinical Manifestations ◽  
The Body ◽  
Feed Mixture ◽  
Milk Productivity ◽  
Structural Disorders ◽  
Increased Sensitivity ◽  
Penicillium Spp

Mycotoxicological monitoring of forages shows that the problem of mycotoxicosis has been relevant for several decades. Minimal doses of mycotoxins in feed lead to a decrease in milk productivity, increased sensitivity to infectious and non-infectious diseases. When several mycotoxins enter the body simultaneously, a synergistic effect develops, causing a significant increase in toxicity. Feed contaminated with several types of fungi and their toxins is dangerous for dairy cattle. It was found that the feed mixture contained the types of associations of Aspergillus spp. fungi + Fusarium; Aspergillus spp. + Penicillium spp. + Mucor spp; Fusarium + Penicillium; Mucor spp. + Fusarium + Ustilaginales. Pathogenetic features of metabolic and morphological changes in highly productive cows with polymycotoxicosis were studied. Feeding food contaminated with various metabolites of mold fungi leads to the development of signs of chronic toxemia in animals. Clinical manifestations are the development of diarrhea and dehydration, with a decrease in milk productivity. Metabolic disorders feature the development of an inflammatory process, metabolic acidosis, hyperfermentonemia, with an increase in the amount of creatinine and urea in the blood serum. Metabolic signs indicate the development of hepatorenal syndrome due to structural disorders of the liver and kidneys. Histological signs of polymicotoxicosis are intracapillary and hemorrhagic glomerulonephritis, hepatocyte micronecrosis, and proliferation of connective tissue stroma cells, which leads to the development of atrophic cirrhosis in the interstitial and circular phases.

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

Acute exudative paraneoplastic polymorphous vitelliform maculopathy in a patient with thymoma, myasthenia gravis, and polymyositis

2021 ◽  
pp. 112067212199404
Author(s):  
He Yu ◽  
Xinyu Ma ◽  
Nianting Tong ◽  
Zhanyu Zhou ◽  
Yu Zhang
Keyword(s):  
Myasthenia Gravis ◽  
Medical Center ◽  
Postoperative Radiotherapy ◽  
Clinical Manifestations ◽  
Subretinal Fluid ◽  
Metastatic Tumor ◽  
The Body ◽  
Pleural Thickening ◽  
History Of ◽  
Clinically Significant

Importance: This is the first reported case of acute exudative paraneoplastic polymorphous vitelliform maculopathy (AEPPVM) in a patient with thymoma, accompanied by myasthenia gravis (MG) and polymyositis. Objective: To examine the pathogenesis of ocular disease in a patient with yolk-like fundus lesions and thymoma, MG, and polymyositis throughout the body based on clinical manifestations, diagnosis, differential diagnosis, and genetic testing to determine the appropriate treatment course. Design, setting, and participants: We describe a 63-year-old woman who presented to our tertiary medical center with a 3-month history of reduced visual acuity in both eyes. Concurrent fundoscopy revealed a 2.0 × 1.7-mm, unifocal, yellow, round vitelliform lesion in the macular region, surrounded by multifocal, shallow, yellow-white pockets of subretinal fluid. The patient’s medical history included thymoma with thymectomy treatment, combined with pericardiectomy and postoperative radiotherapy (20 years prior), followed by a diagnosis of MG with suspect thymic association (15 years prior). Three years prior, the patient had been diagnosed with polymyositis related to paraneoplastic syndrome; 1 year prior, she had been examined for pleural thickening due to suspected metastatic tumor. Results: On her most recent follow-up visit at 3 months after initial diagnosis, the patient was stable with no clinically significant progression in ocular or systemic conditions.

scholarly journals AB1045 CLINICAL, ANALYTICAL AND RADIOLOGICAL CHARACTERISTICS OF A COHORT OF PATIENTS WITH SARCOIDOSIS.

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1814.2-1814
Author(s):  
I. Madroñal García ◽  
C. Aguilera Cros ◽  
L. Mendez Diaz
Keyword(s):  
Systemic Disease ◽  
Immunosuppressive Treatment ◽  
Clinical Manifestations ◽  
Corticosteroid Treatment ◽  
Pulmonary Involvement ◽  
List Type ◽  
Chi Square ◽  
Course Of Disease ◽  
Chronic Course ◽  
Radiological Stage

Background:Sarcoidosis is a systemic disease whose etiology is unknown. It is characterized by the formation of granulomas in any tissue of the organism. Ganglionic, pulmonary and cutaneus involvement is the most prevalent.Objectives:Describe clinical characteristics of a cohort of patients with sarcoidosis diagnosed.Define the association between the ACE’s number at diagnosis, radiological lung stage, treatment and course of disease.Evaluate if the extrapulmonary involvement is related to the course of the disease.Methods:Descriptive retrospective study of patients with S diagnosis treated in our Hospital in 2019. Data were obtained by reviewing medical records. Chi-square tests and parametric tests have been used to establish the differences described in the objectives.Results:102 patients diagnosed with sarcoidosis have been included, (51% females) with an average age of 56±11 years. Suspected diagnosis at the onset of disease was S in 70.6% of patients, followed by suspected lymphoma (20.6%). The average time for the definitive diagnosis of S was 9.5 months. 70.6% of the patients had elevated ACE titles at the beginning. Regarding the clinical manifestations, 18.6% of the patients presented fever at the beginning and 66.7% extrathoracic clinical manifestations. 72.5% have lymph node adenopathies, and in 91% there is thoracic involvement (the most frequent pulmonary stage is stage II). A biopsy was performed in 84.3% of the patients, the lung biopsy being the most performed (52.3%). 88.2% of patients received corticosteroid treatment at the onset of the disease (currently under treatment with corticosteroids 37.3%). 50% of patients are treated with immunosuppressants, Methotrexate was the most used. 5 patients are treated with biological therapy (AntiTNF).Regarding the course of the disease, 51% of the patients have a chronic course, 45.1% are in remission and 3.9% have suffered a relapse of the disease. In this study, no significant relationship was found between the ACE values at the onset of the disease, the pulmonary stage and the course of the disease.According to our data, patients presenting with extrathoracic clinical manifestations need more frequently corticosteroid treatment (p = 0.017) and immunosuppressive treatment (p = 0.001) with respect to patients who do not have an extrathoracic clinic. In addition, patients with an extrathoracic clinic present more frequently a chronic course of the disease than those who do not (p = 0.019).Conclusion:The results described in this study are similar to those found in the literature. The differences found can be explained because patients presenting with extrathoracic clinical manifestations have a more complicated management and need more treatment than those with only pulmonary involvement, even patients with radiological stage I do not usually need treatment, only surveillance.Disclosure of Interests:None declared

scholarly journals Excited delirium syndrome from psychostimulant abuse can mimic a violent scene of death

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shatishraj Jothee ◽  
Mohamed Swarhib Shafie ◽  
Faridah Mohd Nor
Keyword(s):  
Clinical Manifestations ◽  
The Body ◽  
Law Enforcement Agencies ◽  
Manner Of Death ◽  
Case Presentation ◽  
Excited Delirium ◽  
The Common ◽  
Violent Scene ◽  
Naked Body ◽  
Psychostimulant Abuse

Abstract Background Previous reported cases on excited delirium syndrome studied on the common clinical manifestations of the syndrome. The usual forensics implication for the syndrome is that death commonly is associated with restraint procedures by law enforcement agencies; however, not many cases reported highlights the difficulties in attributing a violent scene of death to the syndrome. Case presentation We present a case of a partially naked body found in an apartment unit under suspicious circumstances with multiple injuries. The scene of death was violent, and the body was found with blood wiped all over the floor and walls. Investigators believed a violent crime had occurred, and a suspect was reprimanded. However, upon autopsy, it was found that all injuries were superficially inflicted and were unlikely to have been part of an act of commission or caused his death. Internal examination found no remarkable pathology. Toxicology revealed a presence of psychostimulants, that is, methamphetamine, MDMA, and ethyl alcohol. Reconstruction of events by the witness, who was initially suspected of the ‘murder’, revealed that the injuries and his death could likely be explained by an episode of excited delirium. Conclusion The case highlights the challenges faced when attributing excited delirium syndrome as a cause of death. The syndrome can present with injuries from aggressive or bizarre behaviour, coupled with the destruction of property, which may confuse investigators on the possible manner of death.

CHRONIC ATYPICAL NEUTROPHILIC DERMATOSIS WITH LIPODYSTROPHY AND FEVER (CANDLE SYNDROME) IN PEDIATRIC PRACTICE

2021 ◽  
Vol 100 (2) ◽  
pp. 254-261
Author(s):  
O.G. Sukhovjova ◽  
◽  
I.A. Ivanova ◽  
N.A. Kalugina ◽  
E.S. Zholobova ◽  
...  
Keyword(s):  
Clinical Observation ◽  
Clinical Manifestations ◽  
Maculopapular Rash ◽  
The Body ◽  
Neutrophilic Dermatosis ◽  
Inherited Disease ◽  
Laboratory Activity ◽  
Ubiquitinated Proteins ◽  
Systemic Inflammatory Reaction ◽  
Long Time

The purpose of this publication is to describe the CANDLE syndrome (its etiology, pathogenesis, clinical manifestations) and present a clinical observation. CANDLE syndrome is a rare genetically inherited disease caused by impaired assembly by of the protease, which leads to the accumulation of abnormal (ubiquitinated) proteins in B-lymphocytes, fibroblasts, macrophages and some other cells of the body. As a result, an excessive activation of interferon synthesis occurs and a systemic inflammatory reaction develops with symptoms such as febrile fever, skin syndrome, represented by nodules and maculopapular rash, hepatosplenomegaly, lipodystrophy, amyotrophy, delayed physical development, as well as high laboratory activity. The article describes a clinical observation of a female patient with typical symptoms of CANDLE syndrome. However, due to the rarity of the disease and lack of publications describing it, it has not been possible to diagnose the child for a long time.

Respiratory Acidosis and Alkalosis

2017 ◽  
Author(s):  
Horacio J Adrogué ◽  
Nicolaos E Madias
Keyword(s):  
Carbonic Acid ◽  
Clinical Manifestations ◽  
Respiratory Acidosis ◽  
Respiratory Alkalosis ◽  
Body Fluids ◽  
The Body ◽  
Acid Base ◽  
Acid Base Equilibrium ◽  
Therapeutic Principles ◽  
The Impact

Respiratory acid-base disorders are those disturbances in acid-base equilibrium that are expressed by a primary change in CO2 tension (Pco2) and reflect primary changes in the body’s CO2 stores (i.e., carbonic acid). A primary increase in Pco2 (and a primary increase in the body’s CO2 stores) defines respiratory acidosis or primary hypercapnia and is characterized by acidification of the body fluids. By contrast, a primary decrease in Pco2 (and a primary decrease in the body’s CO2 stores) defines respiratory alkalosis or primary hypocapnia and is characterized by alkalinization of the body fluids. Primary changes in Pco2 elicit secondary physiologic changes in plasma [HCO3ˉ] that are directional and proportional to the primary changes and tend to minimize the impact on acidity. This review presents the pathophysiology, secondary physiologic response, causes, clinical manifestations, diagnosis, and therapeutic principles of respiratory acidosis and respiratory alkalosis.  This review contains 4 figures, 3 tables, and 59 references. Key words: Respiratory acidosis, respiratory alkalosis, primary hypercapnia, primary hypocapnia, hypoxemia, pseudorespiratory alkalosis

Iron Homeostasis and Disorders in Dogs and Cats: A Review

2011 ◽  
Vol 47 (3) ◽  
pp. 151-160 ◽  
Author(s):  
Jennifer L. McCown ◽  
Andrew J. Specht
Keyword(s):  
Iron Deficiency ◽  
Iron Overload ◽  
Iron Homeostasis ◽  
Diagnostic Testing ◽  
Clinical Manifestations ◽  
Essential Element ◽  
The Body ◽  
Deficiency Anemia ◽  
Enzyme Systems ◽  
Living Organisms

Iron is an essential element for nearly all living organisms and disruption of iron homeostasis can lead to a number of clinical manifestations. Iron is used in the formation of both hemoglobin and myoglobin, as well as numerous enzyme systems of the body. Disorders of iron in the body include iron deficiency anemia, anemia of inflammatory disease, and iron overload. This article reviews normal iron metabolism, disease syndromes of iron imbalance, diagnostic testing, and treatment of either iron deficiency or excess. Recent advances in diagnosing iron deficiency using reticulocyte indices are reviewed.

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