THE ROLE OF ENDOGENOUS NEUROPEPTIDES IN THE PATHOGENESIS AND CLINICAL MANIFESTATIONS OF ROSACEA-TIDES IN WOMEN

Rosacea is a common skin disease with a chronic recurrent course. Numerous factors of etiology and pathogenesis of this dermatosis, which may influence the appearance of its clinical signs, have not been elucidated. The aim of the work is to determine the content of endogenous neuropeptides (substance P, leu-enkephalin) in the blood, to find out their role in the development of clinical manifestations of rosacea in women. Materials and methods - 47 women with rosacea were clinically and laboratory examined in whom the levels of substance P and leu-enkephalin in the blood were determined by enzyme-linked immunosorbent assay. Results. Quantitative assessment of the degree of manifestations of clinical signs of local redness of the skin and subsequent symptoms of rosacea (pink acne) in women indicates a complex nature of changes in the skin and reactions of the nervous and endocrine systems. The revealed imbalance in the blood of patients with endogenous neuropeptides with different mechanisms of effector and regulatory action (increase - substance P, decrease - leu-enkephalin) indicates a violation of the mutual compensatory relations of these peptide signaling molecules in different functional systems of the body, and the following symptoms of the disease, which differed in women of reproductive and older age.Conclusions. Imbalance of levels of endogenous neuropeptides with opposite effector and regulatory effects (substance P, leu-enkephalin) may be one of the key pathogenetic factors of the appearance and development of clinical manifestations of rosacea in women given the polypotent mechanisms of action of these biologically active compounds as neurotransmitters and antinociceptive mediators, regulators of emotional and behavioral reactions, pro- and anti-inflammatory factors, coordinators of the hypothalamic-pituitary-gonadal system. Misdiagnosis of such manifestations of the disease as rosacea-tides with «menopausal», «migraine-like» and other types of tides in women dictate the need for a comprehensive examination of such patients.Кey words: rosacea, substance P, ley-enkephalin, women’s.

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Serological monitoring of cattle mycoplasmosis

Siberian Herald of Agricultural Science ◽

10.26898/0370-8799-2019-6-10 ◽

2020 ◽

Vol 49 (6) ◽

pp. 88-95

Author(s):

M. A. Leonova ◽

I. S. Onishchenko ◽

N. Yu. Balybina ◽

I. N. Pen’kova

Keyword(s):

Enzyme Immunoassay ◽

Carrier Phase ◽

Active Form ◽

Clinical Manifestations ◽

Growth Dynamics ◽

The Body ◽

Enzyme Linked Immunosorbent Assay ◽

Novosibirsk Region ◽

Intensive Use ◽

Chronic Course

The results of studying the immune response with persistence of the microorganism of the genus Mycoplasma in the body of cows are presented. The study (2019) was conducted in dairy farms in five districts of Novosibirsk region. Identification of individual specific antibodies of class G to microorganisms of the genus Mycoplasma was carried out in 186 samples of blood serum of cattle. The study was carried out by enzyme immunoassay with the MycoplasmaIgG antibodies ELISA VET kit. In the majority of the studied farms, a prolonged persistence of the microorganism of the genus Mycoplasma was noted. It was found that mycoplasma, having weak immunogenicity, mainly causes a chronic course of the disease. In an enzyme-linked immunoassay, this phenomenon was recorded in dubious reactions in 60.8% of animals. In some farms during the study, a period of reactivation of the disease was noted, which appeared in the transition of the disease from the carrier phase to the active form with clinical manifestations. In the enzyme immunoassay, 7.5% of the animals reacted positively. It was noted that in farms with positively reacting animals, the probability of isolation and spread of the pathogen from sick animals is high. No reaction to the presence of class G antibodies to microorganisms of the genus Mycoplasma was detected in 31.7% of the animals studied. In most farms, the growth dynamics of animals with dubious response was noted to depend on their physiological period. The connection of the duration of cow lactation with the dubious antibody response in an enzyme-linked immunosorbent assay was established. The possibility of connecting this phenomenon with highly intensive use of productive animals, which leads to an increase in stress levels and a decrease in homeostasis and immunity, is shown.

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An Overview of the Trajectory of Brazilian Individuals with 22q11.2 Deletion Syndrome Until Diagnosis.

10.21203/rs.3.rs-751884/v1 ◽

2021 ◽

Author(s):

Isabela Mayá Wayhs Silva ◽

Vera Lúcia Gil-da-Silva-Lopes

Keyword(s):

Health Professionals ◽

Access To Health Care ◽

Clinical Signs ◽

Clinical Manifestations ◽

22Q11.2 Deletion Syndrome ◽

The Body ◽

Deletion Syndrome ◽

Cardiac Malformation ◽

22Q11.2 Deletion ◽

The Moment

Abstract Background: 22q11.2 Deletion Syndrome (22q11.2DS) is a rare disease that has as an important characteristic the clinical heterogeneity. The diversity of organs, regions, and systems of the body that can be affected requires periodic updating of health professionals so that they can recognize this clinical signs as belonging to 22q11.2DS. Updated health professionals are equally important for the appropriate and timely establishment of clinical management for individuals with a positive diagnosis. In this context, this article aimed to map and analyse the access to health care for individuals with 22q11.2DS until the moment of diagnosis. Results: We analysed clinical data of 111 individuals with 22q11.2DS registered in the Brazilian Database on Craniofacial Anomalies (BDCA) from 2008 to 2020. In this study, individuals were diagnosed with a median age of 9 years (mean = 9.7 years). Before genetic investigation, they accessed 68.75% of the international recommended evaluations available at BDCA. Recurrent 22q11.2DS clinical manifestations as delayed neuropsychomotor development, lip and/or palate defects, cardiac malformation and/or hematological/immunological alteration co-occurred in at least 72.06% of individuals. Cardiac malformation was the only clinical alteration that led to a lower median diagnostic age, corresponding to 6.5 years of age with a cardiac malformation versus 11 years of age without a cardiac malformation (p = 0.0006). Conclusions: In Brazil, 22q11.2 DS is under recognized and early diagnosis and management of affected individuals are still a distant reality. In this sense, the identification followed by the correction of obstacles that do not allow this reality are essential to increase life expectancy and improve the quality of life of these individuals in Brazil

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Clinical Characteristics of Pediatric Patients with Ocular Toxocariasis in China

Ophthalmologica ◽

10.1159/000443215 ◽

2016 ◽

Vol 235 (2) ◽

pp. 97-105 ◽

Cited By ~ 7

Author(s):

Yalu Liu ◽

Qi Zhang ◽

Jing Li ◽

Xunda Ji ◽

Yu Xu ◽

...

Keyword(s):

Rural Areas ◽

Clinical Characteristics ◽

Pediatric Patients ◽

Age Of Onset ◽

Age Groups ◽

Clinical Signs ◽

Clinical Manifestations ◽

Signs And Symptoms ◽

Enzyme Linked Immunosorbent Assay ◽

Ocular Toxocariasis

Objective: The aim of the study was to analyze the clinical characteristics of pediatric patients with ocular toxocariasis. Methods: Ocular toxocariasis was diagnosed and treated in 46 children from Shanghai and surrounding provinces. The diagnosis of ocular toxocariasis was confirmed immunologically by performing an enzyme-linked immunosorbent assay on serum and/or intraocular fluid. All pediatric patients and their guardians completed a questionnaire concerning their cases and living habits. Results: The mean age of onset was 6 ± 3 years. Most children (85%) resided in rural areas, and 91% of the children had contact with adult dogs or puppies. At the first visit, visual acuity (VA) was <20/200 in 36 cases, and we detected peripheral granuloma in 36 patients. In our study, the most common signs were vitritis, vitreous strands, and tractional retinal detachment. The Optomap 200Tx device detected granuloma with an 85% sensitivity, which is much higher than that of other techniques. We treated 40 cases (87%) with topical corticosteroids, while 28 patients (61%) were treated with systemic corticosteroids. Only 18 children (39%) required surgical intervention. All patients were examined and treated by the same ophthalmologists. Conclusions: Preschool children in China are more often affected by toxocariasis compared with other age groups. The most common signs included unilateral granuloma and ocular inflammation. In our study, clinical manifestations were severe and complicated. At the first visit, VA was <20/200 in most patients. Ocular toxocariasis was diagnosed on the basis of clinical signs and symptoms; the diagnosis was confirmed by immunological testing. Techniques using the Optomap 200Tx device can facilitate the early detection and lead to better visual prognosis.

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Detection of Surra (trypanosomiasis) positivity in humans in an outbreak area of Indonesia

Medical Journal of Indonesia ◽

10.13181/mji.v28i2.1767 ◽

2019 ◽

Vol 28 (2) ◽

pp. 196-202

Author(s):

Dyah Haryuningtyas Sawitri ◽

April Hari Wardhana ◽

Mohamad Sadikin ◽

Heri Wibowo

Keyword(s):

Clinical Signs ◽

Clinical Manifestations ◽

Protozoan Parasite ◽

Trypanosoma Evansi ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

The Public ◽

Outbreak Area ◽

Blood Sucking ◽

Serological Status

BACKGROUND Surra is an infection caused by a blood protozoan parasite, Trypanosoma evansi, and transmitted by blood-sucking insects. The parasite generally infects only animals; however, it was reported to infect an Indian cattle farmer in 2004, followed by reports of other human cases. The most severe Surra outbreak in Indonesia occurred in Sumba Island during 2010–2012, resulting in the death of more than 2,000 livestock. This study was conducted to explore the serological status of farmers who have intensive contact with their livestock against T. evansi infection in Southwest Sumba district. METHODS A total of 24 serum samples were collected from farmers living in the Surra outbreak area. All sera were tested using both card agglutination test for trypanosomiasis/T. evansi (CATT/T. evansi) and field enzyme-linked immunosorbent assay (FELISA). RESULTS Of the 24 serum samples, 4 (16.7%) samples were seropositive for the antigen T. evansi using both tests. This is the first report of human trypanosomiasis (Surra) in Indonesia. Unfortunately, the clinical manifestations of farmers with positive Surra infection were not reported because all sera samples used in this study were obtained from the Public Health Service with no reports of clinical signs from the respondents. CONCLUSIONS Farmers living in the Surra outbreak area have a high risk of being infected with T. evansi due to their potential frequent contact with Surra vectors. Therefore, T. evansi infection in humans requires attention as it might have the potential to develop as a new emerging zoonotic disease in Indonesia.

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Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.694110 ◽

2021 ◽

Vol 8 ◽

Author(s):

Cun Liu ◽

Yanhan Liu ◽

Xiubo Li ◽

Lin Liang ◽

Shangjin Cui

Keyword(s):

Viral Load ◽

Intramuscular Injection ◽

Vaccine Development ◽

Clinical Signs ◽

Clinical Manifestations ◽

Neutralizing Antibody ◽

Enzyme Linked Immunosorbent Assay ◽

Weaned Piglets ◽

Antibody Levels ◽

Symptoms And Signs

In order to evaluate the pathogenicity of Senecavirus A (SVA) to weaned piglets preliminarily, 28-day-old weaned piglets were challenged with SVA by intramuscular injection. The clinical manifestations, antibody levels, and tissue viral load of infected piglets were detected. The results indicated that the piglets challenged with SVA CH/FuJ/2017 showed drowsiness, lameness, oral blisters, diarrhea, and other clinical signs. Lesions on the hooves were observed. Red spots or plaques were initially observed on the hoof and then developed into blisters that cracked and gradually formed scab. The symptoms and signs were relieved after 8 days post-infection (dpi). The sentinel piglet, feeding together with the challenged piglets, showed similar clinical signs with the challenged piglets after 3 dpi. Monitoring of antibody levels showed that anti-SVA antibody could be detected at 5 dpi by competition enzyme-linked immunosorbent assay (cELISA) method, and neutralizing antibody could be detected after 7 dpi. Analysis of viral tissue distribution and viral load indicated that SVA could replicate in the liver, spleen, lung, kidney, and lymph node. In all, Senecavirus disease was successfully replicated by SVA CH/FuJ/2017 isolate, which verified the clinical manifestations of SVA infection in weaned piglets, and provided a foundation for further SVA pathogenesis and vaccine development.

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Endothelial dysfunction as the key link of chronic diseases pathogenesis

Kazan medical journal ◽

10.17750/kmj2015-659 ◽

2015 ◽

Vol 96 (4) ◽

pp. 659-665 ◽

Cited By ~ 17

Author(s):

Yu S Mel’nikova ◽

T P Makarova

Keyword(s):

Endothelial Dysfunction ◽

Chronic Diseases ◽

Clinical Manifestations ◽

The Body ◽

Biologically Active Substances ◽

Biologically Active ◽

Modern Concept ◽

Chronic Obstructive ◽

Wide Range ◽

Active Substances

Endothelium is the unique «endocrine tree» lining absolutely all cardiovascular system organs of the body. Endothelial cells form a barrier between the blood and tissues, perform a number of important regulatory functions, synthesizing and releasing a wide range of biologically active substances. The strategic location of the endothelium allows it to be sensitive to haemodynamic changes as well as to the signals carried by the blood and signals of underlying tissues. Balanced release of biologically active substances contributes to homeostasis maintenance. The data concerning the multiple mechanisms of endothelium participation in the origin and development of various pathological conditions is accumulated so far. This is not only due to its participation in vascular tone regulation, but also due to the direct influence on atherogenesis, thrombus formation, and protection of the vascular wall integrity. Endothelial dysfunction is considered as a pathological condition of the endothelium based on impaired synthesis of endothelial factors. As a result, endothelium is unable to provide the haemorheological balance of the blood, resulting in disorders of different organs and systems functions. Endothelial dysfunction is a key link in pathogenesis of many diseases and their complications. The role of endothelial dysfunction in the development of chronic diseases such as atherosclerosis, arterial hypertension, chronic heart failure, diabetes mellitus, chronic obstructive pulmonary disease, chronic kidney disease, inflammatory bowel disease, and others has been proven recently. The review provides data on the functions of vascular endothelium and its dysfunction. Types of endothelial dysfunction are described. Modern concept of endothelial dysfunction as the key link of pathogenesis of many chronic diseases is presented. Endothelial dysfunction precedes the development of clinical manifestations of diseases, so the study of the endothelium condition at early stages of the diseases is promising and could be of great diagnostic and prognostic value.

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p-Coumaric Acid, a Major Active Compound of Bambusae Caulis in Taeniam, Suppresses Cigarette Smoke-Induced Pulmonary Inflammation

The American Journal of Chinese Medicine ◽

10.1142/s0192415x18500209 ◽

2018 ◽

Vol 46 (02) ◽

pp. 407-421 ◽

Cited By ~ 10

Author(s):

Woogyeong Kim ◽

Dahae Lim ◽

Jinju Kim

Keyword(s):

Cigarette Smoke ◽

Lung Inflammation ◽

Intestinal Inflammation ◽

Pulmonary Inflammation ◽

The Body ◽

Inflammatory Factors ◽

Enzyme Linked Immunosorbent Assay ◽

Coumaric Acid ◽

Mice Model

[Formula: see text]-coumaric acid ([Formula: see text]-CA) is a common compound found in medicinal herbs, including Bambusae Caulis in Taeniam (BC). It has been used to treat various diseases in China and Korea. Our previous study demonstrated that BC inhibits pulmonary and intestinal inflammation. In the present study, we used cigarette smoke (CS) to induce lung inflammation in vivo, and investigated the anti-inflammatory effects of [Formula: see text]-CA on CS-induced inflammatory mice model. Mice were treated with BC and [Formula: see text]-CA via oral injection 2[Formula: see text]h before CS exposure. The body weight and the inflammatory cells in the bronchoalveolar lavage fluid were measured. The levels of relative inflammatory factors were confirmed by enzyme-linked immunosorbent assay. The lung histological changes were examined by hematoxylin and eosin staining. Also, the protein level of nuclear factor-[Formula: see text]B (NF-[Formula: see text]B) was evaluated by Western blotting. Our results indicated that BC and [Formula: see text]-CA inhibited CS-induced lung inflammation by regulating pro-inflammatory productions such as cytokines, chemokine, protease and NF-[Formula: see text]B. Consequently, these data demonstrated that [Formula: see text]-CA inhibited pulmonary inflammation by suppressing NF-[Formula: see text]B activity, through which pro-inflammatory mediators were regulated. Therefore, [Formula: see text]-CA, which was shown to be a major component of BC, can be considered as a strong therapeutic candidate for treating pulmonary inflammatory diseases.

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Management of cocaine poisoning

10.1093/med/9780199600830.003.0324 ◽

2016 ◽

Author(s):

Nicholas J. Johnson ◽

Judd E. Hollander

Keyword(s):

Chest Pain ◽

Excitatory Amino Acid ◽

Beta Blockers ◽

Clinical Signs ◽

Clinical Manifestations ◽

Signs And Symptoms ◽

The Body ◽

Cardiac Biomarkers ◽

Severe Toxicity ◽

Clinical Signs And Symptoms

Cocaine is powerful central nervous system (CNS) stimulant derived from the coca plant. It affects the body via a number of mechanisms including blockade of fast sodium channels, increased catecholamine release, inhibition of catecholamine reuptake, and increased concentration of excitatory amino acid concentrations in the CNS. It is rapidly absorbed via the aerodigestive, respiratory, gastrointestinal, and genitourinary mucosa, and also may be injected. When injected intravenously or inhaled, cocaine is rapidly distributed throughout the body and CNS, with peak effects in 3–5 minutes. With nasal insufflation, absorption peaks in 20 minutes. Its half-life is approximately 1 hour. Common clinical manifestations include agitation, euphoria, tachycardia, hyperthermia, and hypertension. Chest pain is a common presenting complaint among cocaine users; 6% of these patients will have myocardial infarction. Other life-threatening sequelae include stroke, intracranial haemorrhage, seizures, dysrhythmias, and rhabdomyolysis. Clinical signs and symptoms, as well as severity of intoxication, should dictate the diagnostic evaluation and treatment of cocaine intoxicated patients. If the patient has chest pain, an ECG, chest radiograph, and measurement of cardiac biomarkers should be performed. A brief observation period may be useful in these patients. Many manifestations of cocaine intoxication, including agitation, hypertension, and chest pain, are effectively treated with benzodiazepines. Beta-blockers should be avoided in patients with suspected cocaine intoxication. Special attention should be paid to pregnant patients and those who present after ingesting packets filled with cocaine, as they may exhibit severe toxicity if these packets rupture.

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AB1365-HPR FREQUENCY OF JOINT DAMAGE IN PATIENTS WITH ULCERATIVE COLITIS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.2636 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1969.1-1969

Author(s):

D. Tarasova ◽

L. Shilova ◽

E. Lutaya ◽

E. Korenskaya ◽

M. Koroleva ◽

...

Keyword(s):

Ulcerative Colitis ◽

Clinical Signs ◽

Clinical Manifestations ◽

Joint Damage ◽

The Body ◽

Systemic Autoimmune Disease ◽

Bowel Diseases ◽

Inflammatory Bowel ◽

Mechanisms Of Development ◽

First Time

Background:Ulcerative colitis (UC) is considered as a systemic autoimmune disease with lesions of the colon mucosa. The current of UC is often accompanied by different extra-intestinal manifestations. Their frequency, according to various studies, varies widely – from 25 to 60 %. It is a serious problem that affects the quality of life and the effectiveness of therapy [1, 2]. Rheumatological manifestations, in particular, damage to the joints and spine, are one of the extra-intestinal manifestations and they are of particular importance. To date, the relationship between UC and joint damage has not been fully studied. These diseases can occur independently in the body or have a common autoimmune or inflammatory nature. It is believed that having common pathogenetic mechanisms of development, UC and joint damage can be different clinical forms of the same disease.Objectives:To evaluate the frequency of clinical manifestations of joint damage in patients with ulcerative colitis.Methods:The study was conducted at the gastroenterological Department of the Hospital №25 (Russia, Volgograd). Archived data from the case histories of 69 patients with a confirmed diagnosis of ulcerative colitis were analyzed, including 58 men (30.4%) with an average age of 33.4 years, and 38 women (69.5%) with an average age of 37.6 years.Results:Among 48 patients with UC, extra-intestinal manifestations were detected in 40 (41.6%) patients. A total lesion of the large intestine was found in 20 patients (20.8%), left-sided colitis in 14 (14, 6%), proctosigmoiditis in 6 (6.25%). The diagnosis was made for the first time in 4 patients (4.16%), 36 patients (37.5%) were admitted to the hospital again due to an exacerbation of the disease. Among the extra-intestinal manifestations, joint lesions prevailed: 20 patients (20.8%) showed clinical signs of peripheral arthritis, spondyloarthritis was detected in 8 patients (8.3 %), and 6 patients (6.25 %) had symptoms of unilateral sacroiliitis. 4 (4.16%) patients were diagnosed with nodular erythema. Primary sclerosing cholangitis was detected in two patients (2.08%).Conclusion:The development of extra-intestinal manifestations in UC is largely determined by the course of the disease and the length of the inflammatory process in the colon. More than a third of patients with UC revealed extra-intestinal manifestations, among which the most common signs of joint damage were present, which necessitates timely diagnosis of extra-intestinal manifestations and involvement of a rheumatologist in the management of this category of patients.References:[1]Knyazev O. V. et al. Epidemiologi of inflammatory bowel disease. Yesterdey, today, tomorrow. Eksperimental’naya i Klinicheskaya Gastroenterologiya 2017; 139 (3): 4–12 (In Russ.)[2]Sadygova G.G. Extraintestinal manifestations of inflammatory bowel diseases: arthropathy and arthritis. Ross z gastroenterol gepatol koloproktol 2016; 26(6):101-5 (In Russ.)Disclosure of Interests:None declared

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New Insights into the Activities of D-Chiro-Inositol: A Narrative Review

Biomedicines ◽

10.3390/biomedicines9101378 ◽

2021 ◽

Vol 9 (10) ◽

pp. 1378

Author(s):

Riccardo Gambioli ◽

Mario Montanino Oliva ◽

Maurizio Nordio ◽

Alfonsina Chiefari ◽

Giulia Puliani ◽

...

Keyword(s):

Single Molecule ◽

Natural Compound ◽

Embryo Implantation ◽

Hydroxysteroid Dehydrogenase ◽

The Body ◽

Inflammatory Factors ◽

Insulin Resistant ◽

Tnf Α ◽

Regulatory Effects

D-chiro-inositol (DCI) is a natural compound detectable in cell membranes, which is highly conserved as a biological signaling molecule. In mammals, its function is primarily characterized in the intracellular transduction cascade of insulin. In particular, insulin signal promotes the release of pivotal DCI-containing molecules. In fact, impaired release of DCI is a common feature of insulin-resistant tissues, and insulin-sensitizing pharmaceuticals induce higher concentrations of free DCI. Moreover, it also plays important roles in several other processes. DCI is involved in the regulation of steroidogenesis, due to its regulatory effects on steroidogenic enzymes, including 17α-hydroxylase, 3β-hydroxysteroid dehydrogenase, and aromatase. Such regulation of various enzymes indicates a mechanism by which the body regulates different processes via a single molecule, depending on its concentration. DCI also reduces the expression of integrin β3, which is an adhesion molecule involved in embryo implantation and cellular phenomena such as survival, stemness, and invasiveness. In addition, DCI seems to have important anti-inflammatory activities, like its 3-O-methyl-ether, called pinitol. In vitro evidence demonstrates that treatment with both compounds induces a reduction in pro-inflammatory factors—such as Nf-κB—and cytokines—such as TNF-α. DCI then plays important roles in several fundamental processes in physiology. Therefore, research on such molecule is of primary importance.

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