Selected haematological and biochemical indices in donkeys in the Czech and Slovak Republics

The aim of this study was to establish normal reference values of biochemical and haematological indices of donkeys in the Czech and Slovak Republics. Blood samples were obtained from 112 clinically healthy donkeys (37 males and 75 females). The haematological indices examined were: red blood cells, white blood cells, haemoglobin concentration, haematocrit, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, platelets, segmented neutrophils, neutrophil bands, lymphocytes, monocytes, eosinophils and basophils. The biochemical properties examined were: total protein, albumin, creatinine, alkaline phosphatase, aspartate aminotransferase, creatine kinase, gamma-glutamyl transferase, lactate dehydrogenase, triacylglycerols, cholesterol, calcium, phosphorus, potassium, sodium, lactate. The results reported in this study could serve as reference ranges for the donkey population in the Czech and Slovak Republics.

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Effect of Quercetin on Haematobiochemical and Histological Changes in the Liver of Polychlorined Biphenyls-Induced Adult Male Wistar Rats

Journal of Biomarkers ◽

10.1155/2013/960125 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 12

Author(s):

Kandaswamy Selvakumar ◽

Senthamilselvan Bavithra ◽

Sekaran Suganya ◽

Firdous Ahmad Bhat ◽

Gunasekaran Krishnamoorthy ◽

...

Keyword(s):

Blood Cells ◽

Hematological Parameters ◽

White Blood Cells ◽

Density Lipoprotein ◽

Protective Role ◽

Low Density Lipoproteins ◽

Gamma Glutamyl Transferase ◽

Low Density ◽

Very Low Density Lipoproteins ◽

Glutamyl Transferase

Polychlorinated biphenyls exposure damages the rat liver cells. Hematological parameters such as hemoglobin, packed cell volume, red-blood cells, white-blood cells, neutrophils, platelet counts, and RBC indices were significantly decreased. Polymorphs, eosinophil counts, and erythrocyte sedimentation rate were significantly increased. Serum liver enzymes such as aspartate transaminase, alanine transaminase, alkaline phosphatase, and gamma-glutamyl transferase were increased by PCBs treatment. Serum lipid profiles such as cholesterol, triglycerides, low-density lipoproteins and very-low-density lipoproteins were increased in PCBs-treated rats. High-density lipoprotein, total protein, albumin, globulin levels, and albumin/globulin ratio were also decreased after PCB exposure. Then levels of sodium, potassium, chloride, and bicarbonate were also altered. Serum glucose levels were increased along with total bilirubin after PCBs exposure. Simultaneous quercetin supplementation significantly protected the PCBs-induced changes of hematobiochemical parameters. Thus, quercetin shows a protective role against PCBs-induced alterations in the hematological and biochemical parameters.

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Safety evaluation of the aqueous extract of Leonotis leonurus shoots in rats

Human & Experimental Toxicology ◽

10.1177/0960327108099533 ◽

2008 ◽

Vol 27 (11) ◽

pp. 837-843 ◽

Cited By ~ 19

Author(s):

V Maphosa ◽

PJ Masika ◽

AA Adedapo

Keyword(s):

Acute Toxicity ◽

Aqueous Extract ◽

Blood Cells ◽

Chronic Toxicity ◽

White Blood Cells ◽

Female Rats ◽

Gamma Glutamyl Transferase ◽

Histopathological Changes ◽

Glutamyl Transferase ◽

Leonotis Leonurus

The aqueous extract from Leonotis leonurus (L) R.Br. (Lamiaceae) shoots was evaluated in female rats for its acute, sub acute, and chronic toxicity together with hematological, biochemical, and histopathological changes. In the acute toxicity test, the extract caused death in animals receiving 3200 mg/kg dose. The extract also caused significant ( P < 0.05) changes in red blood cells, packed cell volume, hemoglobin concentration, mean corpuscular volume, platelets, white blood cells and its differentials at doses of 1600 mg/kg in sub-acute toxicity and in as low as 200 mg/kg in chronic toxicity. In biochemical parameters, the extract caused a significant ( P < 0.05) decrease in the levels of urea and creatinine at 1600 mg/kg dose and a significant ( P < 0.05) reduction in urea, total bilirubin, total protein, albumin, globulin, glutamine transference gamma-glutamyl transferase (GGT), and alanine transminase in the 400 mg/kg dose in chronic toxicity. Changes were also noted in body weights, but no significant changes were observed in the levels of electrolytes (sodium, potassium, and chloride). Clinico-pathologically, starry hair coat, respiratory distress, and mortality were recorded. The extract also caused various histopathological changes in the organs. The study concluded that farmers need to exercise caution in the use of the plant for medicinal purposes.

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Effects of Age, Breed and Sex on Haematological Parameters of Growing Omani Goat Breeds

Sultan Qaboos University Journal for Science [SQUJS] ◽

10.24200/squjs.vol21iss2pp82-88 ◽

2016 ◽

Vol 21 (2) ◽

pp. 82

Author(s):

Nur El Huda I. Osman ◽

Rashid M. Al-Busaidi ◽

Eugene H. Johnson

Keyword(s):

Cell Volume ◽

Blood Cells ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Blood Parameters ◽

Haematological Parameters ◽

Mean Cell Volume ◽

Sultan Qaboos University ◽

Breed Differences ◽

Agricultural Experiment

Sixty seven healthy growing goat kids of three Omani breeds, born and raised in Sultan Qaboos University Agricultural Experiment Station, were used to study the effects of age, breed and sex on haematological parameters. Blood samples were collected at several times from the first to 25 weeks to study the following parameters: Red Blood Cells (RBCs), White Blood Cells (WBCs), Packed Cell Volume (PCV), Haemoglobin (Hb), Mean Cell Volume (MCV), Mean Cell Haemoglobin Concentration (MCHC) and Mean Cell Haemoglobin (MCH). At birth, MCV and MCH levels were significantly higher in Batina (BAT) kids than both Jabal Akhdar (JA) and Dhofari (DOF), but there were no breed differences in the rest of the haematological values. There were significant breed differences in RBC, MCH, WBC, MCV at different ages, with Hb and PCV being highly significant at all ages. All blood parameters changed with time but showed different patterns in all breeds. There was no effect of sex on RBC, MCHC, PCV, Hb or WBC, but female kids had significantly higher MCH and MCV values at week 12. This study indicates that haematology parameters in Omani goat kids could be affected by age, breed and sex.

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Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes

Journal of Feline Medicine and Surgery ◽

10.1177/1098612x18798841 ◽

2018 ◽

Vol 21 (8) ◽

pp. 732-740 ◽

Cited By ~ 2

Author(s):

Eva Spada ◽

Roberta Perego ◽

Luciana Baggiani ◽

Daniela Proverbio

Keyword(s):

Whole Blood ◽

Blood Cells ◽

Morphological Changes ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

In Vivo Studies ◽

Mean Cell Volume ◽

Distribution Width

ObjectivesDespite the increasing availability of feline blood collected and stored for transfusion purposes, few studies have been performed on feline blood units. The aim of this prospective in vitro study was to evaluate haematological and morphological changes in feline blood cells in whole blood units between collection and end of storage.MethodsHaematological examination (red blood cells [RBCs], haemoglobin, haematocrit, red cell distribution width, mean cell volume, mean cell haemoglobin concentration, mean cell haemoglobin, white blood cells [WBCs] and platelet [PLT] count) was performed on 40 non-leukoreduced feline whole blood units at the time of collection (day[D]0) and after storage (D35). The blood was collected into citrate–phosphate–dextrose–adenine anticoagulant-preservative solution using an open system in a veterinary blood bank and stored for 35 days at 4 ± 2°C. Twenty of these feline whole blood units were also analysed for blood cell morphology (normal RBCs, macrocytes, echinocytes, spherocytes, schistocytes, lysed RBCs, RBCs with Heinz bodies and recognisable WBC and PLT count). Differences between the two examination times were statistically analysed.ResultsThere was a statistically significant decrease in WBC and PLT counts after storage at D35 ( P <0.0001 for both). The most significant cellular morphological changes after storage were an increase in echinocyte count ( P = 0.0001), and lysed RBCs ( P <0.0001), and a decrease in normal RBCs ( P <0.0001). Recognisable WBCs – mainly lymphocytes – were present at the end of storage.Conclusions and relevanceThis study showed that significant morphological changes occur in RBCs in feline blood units during storage for 35 days. In vivo studies are required to establish if these changes could affect the ability of stored RBCs to circulate and provide adequate oxygen delivery after transfusion.

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Application of a Machine Learning Technology in the Definition of Metabolically Healthy and Unhealthy Status: A Retrospective Study of 2567 Subjects Suffering from Obesity with or without Metabolic Syndrome

Nutrients ◽

10.3390/nu14020373 ◽

2022 ◽

Vol 14 (2) ◽

pp. 373

Author(s):

Davide Masi ◽

Renata Risi ◽

Filippo Biagi ◽

Daniel Vasquez Barahona ◽

Mikiko Watanabe ◽

...

Keyword(s):

Machine Learning ◽

Body Fat ◽

Blood Cells ◽

White Blood Cells ◽

Surrogate Marker ◽

Upper Body ◽

Public Health Issue ◽

Gamma Glutamyl Transferase ◽

Metabolically Healthy ◽

Glutamyl Transferase

The key factors playing a role in the pathogenesis of metabolic alterations observed in many patients with obesity have not been fully characterized. Their identification is crucial, and it would represent a fundamental step towards better management of this urgent public health issue. This aim could be accomplished by exploiting the potential of machine learning (ML) technology. In a single-centre study (n = 2567), we used an ML analysis to cluster patients with metabolically healthy (MHO) or metabolically unhealthy (MUO) obesity, based on several clinical and biochemical variables. The first model provided by ML was able to predict the presence/absence of MHO with an accuracy of 66.67% and 72.15%, respectively, and included the following parameters: HOMA-IR, upper body fat/lower body fat, glycosylated haemoglobin, red blood cells, age, alanine aminotransferase, uric acid, white blood cells, insulin-like growth factor 1 (IGF-1) and gamma-glutamyl transferase. For each of these parameters, ML provided threshold values identifying either MUO or MHO. A second model including IGF-1 zSDS, a surrogate marker of IGF-1 normalized by age and sex, was even more accurate with a 71.84% and 72.3% precision, respectively. Our results demonstrated high IGF-1 levels in MHO patients, thus highlighting a possible role of IGF-1 as a novel metabolic health parameter to effectively predict the development of MUO using ML technology.

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Haematological and serum biochemical indices of broiler chickens fed sorghum SK- 5912 ( , L. Moench) variety as a replacement for maize

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i3.408 ◽

2020 ◽

Vol 45 (3) ◽

Author(s):

O.A. Lakurbe ◽

U.D. Doma ◽

K.M. Bello ◽

M. Abubakar

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

Broiler Chickens ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Blood Analysis ◽

Serum Biochemical Parameters ◽

Feeding Trial ◽

Serum Biochemical ◽

Biochemical Indices

An eight-week feeding trial was carried out with three hundred unsexed day-old (Marshall) broilers to investigate the effect of sorghumSK-5912 variety as replacement for maize on the haematological and serum biochemical parameters of broiler chickens. Five experimental diets were formulated with SK-5912 replacing maize at 0, 25, 50, 75 and 100% in the diet and labelled as diets 1 (control), 2, 3, 4 and 5, respectively for both starter (23% CP) and finisher (20% CP) phases. The day-old chicks were randomly allotted to the dietary treatments with four replications each and 15 birds per replicate in a completely randomized design (CRD). Feed and water were supplied ad libitum and the experiment lasted eight weeks. At the end of the feeding trial eight birds per treatment were randomly selected and blood samples collected for haematological and serum biochemical analyses. Results from blood analysis showed no treatment effect on Packed Cell Volume (PCV), White Blood Cells (WBC), Red Blood Cells (RBC) counts, haemoglobin concentration (Hb) and corpuscular values. Similarly, there were no significant treatment effects among all the groups for serum biochemical indices. It can therefore be concluded that Sorghum SK-5912 can replace maize in the diets of broiler chickens without adverse effects on the haematological and serum biochemical parameters of the birds.

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Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2020.2.5.133 ◽

2020 ◽

Vol 2 (5) ◽

Author(s):

Y. Garba ◽

E. A. Adeola

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Serum Biochemical Parameters ◽

Corpuscular Haemoglobin ◽

Randomized Design ◽

Serum Biochemical ◽

Mean Corpuscular Haemoglobin ◽

Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

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The prevalence of leukocyte abnormalities among Sudanese patients with sickle cell disease

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.9.1.0026 ◽

2021 ◽

Vol 9 (1) ◽

pp. 262-267

Author(s):

Tarig Osman Khalafallah Ahmed ◽

Ekhlas Alrasheid Abu Elfadul ◽

Ahmed A. Agab Eldour ◽

Omer Ibrahim Abdallah Mohammed

Keyword(s):

Sickle Cell Disease ◽

Cell Volume ◽

Sickle Cell ◽

Blood Cells ◽

White Blood Cells ◽

Blood Film ◽

Cell Disease ◽

Mean Cell Volume ◽

Blood Disorder ◽

Age Range

Sickle cell disease (SCD) is an inherited blood disorder that affects red blood cells. The study was conducted in Elobied town during the period May 2011 to September 2011. The aim of this study is to detect the abnormalities of leucocytes among sickle cell anemic patients. 40 sickle cell anemic patients; age range between 8 months to 23 years. Blood sample was taken for all patients and the laboratory investigation were performed using automated estimation for: hemoglobin (Hb), Packed cell volume (PCV), red cell count (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell concentration (MCHC), and total white blood cells, comment on blood film using manual methods. The conclusion of this study there is increase in total white blood cells with shift to left in neutrophil precursor in sickle cell patients with complications ,the most immature cells are band form, myelocytes and metamyelocytes, and there also lymphocytosis and neutrophilia which has been increases in response to infections.

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Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes

Animals ◽

10.3390/ani10071209 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1209 ◽

Cited By ~ 1

Author(s):

Muhammad Abdul Basit ◽

Arifah Abdul Kadir ◽

Teck Loh ◽

Saleha Abdul Aziz ◽

Annas Salleh ◽

...

Keyword(s):

Growth Performance ◽

Blood Cells ◽

Broiler Chickens ◽

White Blood Cells ◽

Serum Levels ◽

Linear Increase ◽

The Body ◽

Leaf Meal ◽

Serum Activity ◽

Haematological Indices

This research was conducted to estimate the effects of Persicaria odorata leaf meal (POLM) on haematological indices, serum biochemical attributes, and internal organs parameters, including histomorphological features of the liver, in broiler chickens. A total of 120 one-day-old male broiler chicks (Cobb-500) were randomly allocated into four experimental groups. The dietary treatments were basal diet (BD), which served as the control (C), along with BD + 2 g/kg POLM (Po2), BD + 4 g/kg POLM (Po4), BD + 8 g/kg POLM (Po8), which were the supplemented groups. The body weight gain (BWG) showed a linear increase and feed conversion ratio (FCR) showed a linear decrease with increasing POLM dosage at day 42 (p ˂ 0.05) and for the overall growth performance period (p ˂ 0.01). On day 21 and day 42, the values of red blood cells (RBCs), white blood cells (WBCs), haemoglobin (Hb), and packed cell volume (PCV) showed linear increases (p ˂0.05) as the dosage of POLM increased in the diet. On day 21, dietary supplementation of POLM linearly decreased (p ˂ 0.05) the serum activity of alkaline phosphatase (ALP), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), and serum levels of urea and creatinine. On the other hand, serum levels of total protein (TP), albumin, and globulin showed a linear increase (p ˂ 0.05) as the POLM dosage increased. On day 42, the serum activity of AST and ALT and serum levels of glucose, cholesterol, triglycerides, urea, and creatinine showed linear decreases (p ˂ 0.05) with increased levels of POLM in the diet. However, POLM supplementation linearly increased (p ˂ 0.05) the serum levels of TP and globulin. Dietary inclusion of POLM did not influence the organ parameters and showed no adverse effects on the liver histomorphology. In conclusion, supplementation of POLM increased the growth performance, improving haematological indices and serum biochemistry profiles of broiler chickens without any deleterious effects on the liver histomorphology. The results of the present study provide evidence that POLM can be safely used at a dose rate of 8 g/kg of feed as an alternative to conventional antimicrobial growth promoters (AGPs).

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Modulatory role of betulinic acid in N-nitrosodimethylamine-induced hepatorenal toxicity in male Wistar rats

Human & Experimental Toxicology ◽

10.1177/0960327116661399 ◽

2016 ◽

Vol 36 (7) ◽

pp. 734-743 ◽

Cited By ~ 3

Author(s):

GE Adeleke ◽

OA Adaramoye

Keyword(s):

Dna Fragmentation ◽

Betulinic Acid ◽

Wistar Rats ◽

Saline Control ◽

Gamma Glutamyl Transferase ◽

Toxic Agent ◽

Polychromatic Erythrocytes ◽

Male Wistar Rats ◽

Glutamyl Transferase ◽

Biochemical Indices

N-nitrosodimethylamine (NDMA) is a toxicant found in foods and drinking water. Several synthetic agents used in alleviation of NDMA toxicity have been associated with serious side effects. Therefore, a safe and less toxic agent is desirable. In this study, betulinic acid (BA), a triterpenoid antioxidant, is proposed as a better and alternative agent to modulate NDMA-induced toxicity. Twenty-four Wistar rats were assigned into four groups of six rats each and treated with normal saline (control), BA (25 mg/kg), NDMA (5 mg/kg) and (BA + NDMA). BA was given by oral gavage for 14 consecutive days, while NDMA was administered intraperitoneally on days 7 and 12. Results showed that administration of NDMA significantly ( p < 0.05) elevated the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase and gamma-glutamyl transferase by 51%, 48% and 81%, respectively. Also, NDMA intoxication significantly ( p < 0.05) increased the levels of serum urea and creatinine by 64% and 82%, respectively, and decreased urinary creatinine by 67%. In addition, administration of NDMA significantly ( p < 0.05) increased the levels of hepatic and renal DNA fragmentation by 44% and 61%, respectively, relative to control. The number of micronucleated polychromatic erythrocytes (mnPCEs) in NDMA-treated rats (11.1 ± 2.6 mnPCE/1000PCE) was significantly higher than control (4.3 ± 1.1 mnPCE/1000 PCE). Immunohistochemistry revealed strong expressions of Bcl-2 and nuclear p53 in NDMA-intoxicated rats. Interestingly, pretreatment with BA significantly ( p < 0.05) ameliorated NDMA-induced changes in serum biochemical indices, mnPCEs, DNA fragmentation and expressions of Bcl-2 and p53 proteins. These findings suggest that BA protects against NDMA-induced toxicity via anti-oxidative and anti-apoptotic activities.

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