scholarly journals CHARACTERISTIC BIOACTIVE COMPOUND OF THE MOLLUSC SYMBIOTIC BACTERIA BY USING GC-MC

2010 ◽  
Vol 2 (2) ◽  
Author(s):  
Delianis Pringgenies
Keyword(s):  
Nitrous Oxide ◽  
Acetic Acid ◽  
Nitrogen Oxide ◽  
Pathogenic Bacteria ◽  
Bioactive Compound ◽  
Isobutyric Acid ◽  
Symbiotic Bacteria ◽  
Propanoic Acid ◽  
Butanoic Acid ◽  
Bioactive Substances

<p>It has been discovered that mollusca produce a secondary metabolite and in the same time bear its important role in its ecosystems so became a strategic target for the development of noble bioactive substances for marine pharmacology. The current study of mollucs symbiotic bacteria showed that from species of Conus miles, Stramonita armigera, Cymbiola vespertilo and from based on screening of symbiotic bacteria in the Mollusc toward some bacteria , 3 isolates had been had good performance in inhibiting the grow of bacteria and to be the best candidates for a new antibiotic based on result of screening consistency. Size and character inhibiting zone resulted toward test bacteria were TCM, TCAand TOV. The research aims to current study of characteritic of symbiotic bacteria from mollucs that produce a new anti-pathogenic bacteria by using GC-MS method. GC-MS result showed that fraction TCM-6.1 consist of some compounds, that are Nitrogen oxide (N2O) (CAS) Nitrous oxide; Acetic acid (CAS) Ethylic acid; Propanoic acid,2-methyl-(CAS) Isobutyric acid and fraction TOV12.16 consist of compound such as Propanoic acid,2-methyl-(CAS)Isobutyric acid; Butanoic acid, 2-methyl-(CAS) 2-Methylbutanoid acid then fraction TSA8.7 consist of 1,2-Propadiene (CAS) Allene. The research pointed towards the three active symbiotic bacteria seems to be promising since this three candidates potential result for the development of a new antibiotic.</p><p>Keywords: Bacteria simbiont, mollusc, anti-bacteria, bioaktif compound</p>

scholarly journals CHARACTERISTIC BIOACTIVE COMPOUND OF THE MOLLUSC SYMBIOTIC BACTERIA BY USING GC-MC

2010 ◽  
Vol 2 (2) ◽  
Author(s):  
Delianis Pringgenies
Keyword(s):  
Nitrous Oxide ◽  
Acetic Acid ◽  
Nitrogen Oxide ◽  
Pathogenic Bacteria ◽  
Bioactive Compound ◽  
Isobutyric Acid ◽  
Symbiotic Bacteria ◽  
Propanoic Acid ◽  
Butanoic Acid ◽  
Bioactive Substances

It has been discovered that mollusca produce a secondary metabolite and in the same time bear its important role in its ecosystems so became a strategic target for the development of noble bioactive substances for marine pharmacology. The current study of mollucs symbiotic bacteria showed that from species of Conus miles, Stramonita armigera, Cymbiola vespertilo and from based on screening of symbiotic bacteria in the Mollusc toward some bacteria , 3 isolates had been had good performance in inhibiting the grow of bacteria and to be the best candidates for a new antibiotic based on result of screening consistency. Size and character inhibiting zone resulted toward test bacteria were TCM, TCAand TOV. The research aims to current study of characteritic of symbiotic bacteria from mollucs that produce a new anti-pathogenic bacteria by using GC-MS method. GC-MS result showed that fraction TCM-6.1 consist of some compounds, that are Nitrogen oxide (N2O) (CAS) Nitrous oxide; Acetic acid (CAS) Ethylic acid; Propanoic acid,2-methyl-(CAS) Isobutyric acid and fraction TOV12.16 consist of compound such as Propanoic acid,2-methyl-(CAS)Isobutyric acid; Butanoic acid, 2-methyl-(CAS) 2-Methylbutanoid acid then fraction TSA8.7 consist of 1,2-Propadiene (CAS) Allene. The research pointed towards the three active symbiotic bacteria seems to be promising since this three candidates potential result for the development of a new antibiotic.Keywords: Bacteria simbiont, mollusc, anti-bacteria, bioaktif compound

scholarly journals Cellulose Compound of Cacao Waste and Chemical Composition of Cacao Vinegar with GC-MS Method

2018 ◽  
Vol 2 (3) ◽  
pp. 191 ◽  
Author(s):  
Mohammad Wijaya. M ◽  
Muhammad Wiharto ◽  
Muhammad Anwar
Keyword(s):  
Acetic Acid ◽  
Chemical Composition ◽  
Pyrolysis Temperature ◽  
Propanoic Acid ◽  
Cellulose Content ◽  
Butanoic Acid ◽  
Liquid Smoke ◽  
Eds Analysis ◽  
Shell Waste ◽  
Cocoa Shell

<p>Potential of cacao resources at Indonesian is very dependent on the production of cacao produced by farmers and plantation availability. However, the cacao processing results are still not optimal to increase the cacao production. This is due to the disruption of pests and the number of cacao farmers over land functions for fast growing plants. Processing cacao produced cacao waste and with the use of pyrolysis technology is able to cope with the accumulation of plantation waste. This combustion results in liquid smoke of cacao (cacao vinegar) into distillate, charcoal. This study used pyrolysis temperature between 100-500 °C. The aim of this research is to analyze the cocoa waste and the results are cellulose content 17,27%, lignin 52,02% and hemicellulose 19,56%. The results of GC-MS analysis for cacao vinegar of Distric Wajo are acetic acid, n butane, methyl ester, propanoic acid, butanoic acid, cyclopenanone, 2 methyl pyridine, acetyloxy 2 propanone, butyrolactone, tetrahydro 2 furan methanol, 2,3 dimethyl 2 cyclopentene 1 on and Mequinol. The water content of the charcoal of cacao shell from Wajo district is 3.42%. The analysis results of the bound carbon content of activated charcoal of cacao shell is 54.45%. The EDS analysis for cacao shell from Wajo district resulted in C: 61.12%, O: 36.65%, Si: 0.59%, P: 1.48% and Al: 0.17%. Utilization of cocoa shell waste using pyrolysis technology can reduce carbon emissions to the environment. So that the development of everything can continue and the sustainability of forest remain sustainable.</p><p> </p>

scholarly journals P024 Plasma acetic acid, propanoic acid, and isobutyric acid are associated with treatment response in pouchitis patients treated with antibiotics

2019 ◽  
Vol 13 (Supplement_1) ◽  
pp. S097-S098
Author(s):  
J Segal ◽  
M Sarafian ◽  
J I Serrano Contreras ◽  
A Pechlivanis ◽  
Y Siaw ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Treatment Response ◽  
Isobutyric Acid ◽  
Propanoic Acid

scholarly journals PELUANG ZAT BIOAKTIF TANAMAN SEBAGAI ALTERNATIF IMBUHAN PAKAN ANTIBIOTIK PADA AYAM / The Opportunities of Plants Bioactive Compound as an Alternative of Antibiotic Feed additive on Chicken

2019 ◽  
Vol 38 (2) ◽  
pp. 96
Author(s):  
Tiurma - Pasaribu
Keyword(s):  
Bioactive Compounds ◽  
Feed Efficiency ◽  
Pathogenic Bacteria ◽  
Negative Impact ◽  
Raw Materials ◽  
Animal Health ◽  
Bioactive Compound ◽  
Feed Additives ◽  
Feed Additive ◽  
Bioactive Substances

<p>Bioactive compounds (phenols, tannins, flavonoids, essential oils, curcumin, saponins, phyllanthin) have the ability as an antibacterial or antifungal. Feed additives are feed raw materials that do not contain nutrients, however, it may increase productivity, quality of livestock products (meat, eggs, milk, skin, feathers), the feed efficiency and to improve animal health or resistance of disease. Feed additives that are widely used in the livestock industry include antibiotics, antioxidants, antifungals, emulsifiers, and binders. The aim of using antibiotics is to reduce the population of pathogenic microbes or disturbing microbes in the digestive tract. Antibiotics have been banned for used because it can cause resistance to pathogenic bacteria or intestinal microflora which has a negative impact on consumers. To improve feed efficiency in poultry and to produce higher quality products, healthy and safe for consumption, the antibiotic could be replaced with plant bioactive compound. The aims of this review is to describe the role of plant bioactive compounds as feed additive to replace antibiotic for chickens. Some of plant bioactive substances that can be used as feed additives include phenols, curcumin, saponins, tannins, phenols, flavonoids, alkaloids. Bioactive substances from plants have several functions including inhibiting the growth of bacteria or fungi, increasing endurance, as an adjuvant, and preventing fat oxidation. It can be concluded that bioactive substances from plants have potential as feed additives which have the ability as antibacterial, antifungal, antioxidant, immunostimulator, and adjuvant.</p><p>Keywords: bioactive compound, plants, feed additives, chicken </p><p> </p><p><strong>Abstrak</strong></p><p> Zat bioaktif (fenol, tanin, flavonoid, minyak atsiri, curcumin, saponin, phyllanthin) memiliki kemampuan sebagai antibakteri atau antifungi. Imbuhan pakan adalah bahan baku pakan yang tidak mengandung nutrisi, namun dapat meningkatkan produktivitas, kualitas produk ternak (daging, telur, susu, kulit, bulu), efisiensi penggunaan pakan dan meningkatkan kesehatan hewan atau ketahanan terhadap penyakit. Imbuhan pakan yang banyak digunakan dalam industri peternakan termasuk antibiotik, antioksidan, antifungi, pengemulsi, dan pengikat (binder). Tujuan penggunaan antibiotik adalah untuk mengurangi populasi mikroba patogen atau mikroba yang mengganggu di saluran pencernaan. Antibiotik telah dilarang untuk digunakan karena dapat menyebabkan resistensi terhadap bakteri patogen atau mikroflora usus yang memiliki dampak negatif pada konsumen. Untuk meningkatkan efisiensi pakan pada unggas dan menghasilkan produk berkualitas tinggi, sehat dan aman untuk dikonsumsi, antibiotik dapat diganti dengan zat bioaktif tanaman. Tujuan dari ulasan ini adalah untuk menggambarkan peran zat bioaktif tanaman sebagai pengganti imbuhan pakan antibiotik pada ayam. Beberapa zat bioaktif tanaman yang dapat digunakan sebagai imbuhan pakan termasuk fenol, kurkumin, saponin, tanin, fenol, flavonoid, alkaloid. Zat bioaktif dari tanaman memiliki beberapa fungsi antara lain menghambat pertumbuhan bakteri atau jamur, meningkatkan daya tahan tubuh, sebagai bahan adjuvan dan mencegah oksidasi lemak. Dapat disimpulkan bahwa zat bioaktif dari tanaman berpotensi sebagai imbuhan pakan yang memiliki kemampuan sebagai antibakteri, antifungi, antioksidan, imunostimulator, dan adjuvant.</p><p>Kata kunci: Zat bioaktif, tanaman, imbuhan pakan, ayam </p>

scholarly journals Bakteri Simbion Gastropoda Pleuroploca trapesium Dari Perairan Ternate, Sebagai Alternatif Antibakteri MDR (Bacterial Symbiont Gastropoda Pleuroploca trapezium from Ternate, as Alternative Antibacterial MDR)

2014 ◽  
Vol 19 (1) ◽  
pp. 55
Author(s):  
Delianis Pringgenies ◽  
Person Pesona Renta
Keyword(s):  
16S Rdna ◽  
Pathogenic Bacteria ◽  
Dna Amplification ◽  
Sensitivity Test ◽  
Symbiotic Bacteria ◽  
Drug Resistant ◽  
16S Rdna Sequence Analysis ◽  
Close Relationship ◽  
Antibiotic Compounds ◽  
Bacterial Sensitivity

Bakteri yang resisten terhadap beberapa jenis antibakteri ini dikenal dengan bakteri multi drug resistant (MDR).Untuk mengatasi permasalahan tersebut, perlu dilakukan pencarian senyawa antibiotik baru yang lebih efektif dan efisien dalam mengatasi permasalahan bakteri MDR. Penelitian bertujuan untuk mengetahui potensi bakteri yang bersimbiosis dengan gastropoda Pleuroploca trapezium sebagai sumber antibakteri MDR. Sampel Moluska dikoleksi dari perairan Ternate, Maluku. Tahapan penelitian meliputi isolasi bakteri, skrining  bakteri simbion yang potensi sebagai anti bakteri MDR, uji antibakteri, isolasi bakteri patogen klinis MDR; uji sensitivitas anti-bakteri, ekstraksi, amplifikasi dan sekuensing DNA. Hasil 16S urutan r-DNA dianalisis dan diedit menggunakan program Genetix dan diikuti dengan analisis urutan 16S rDNA. Hasil menunjukkan bahwa terdapat 19 isolat bakteri dengan 5 bakteri aktif yang berasosiasi dengan Pleuroploca trapezium. Berdasarkan besarnya zona hambat yang dibentuk dan konsistensi munculnya zona hambatan, isolat terbaik adalah TPT 4.7. Isolat ini memiliki hubungan yang dekat dengan Paracoccus  sp. MBIC4019 dengan homologi sebesar 95% yang menunjukkan kekerabatan ditingkat genus. Hasil penelitian ini memberikan harapan adanya potensi besar sebagai bahan antibakteri baru. Kata kunci: antibakteri, simbion, Pleuroploca trapezium, multi drugs resistantThe bacteria resistant to some antibiotics are known as multi drug resistant (MDR). To overcome the problem, it is needed to search for a new antibiotic compounds more effectively and efficiently. This study aims to identify potential from symbionts of Pleuroploca trapezium as a source of antibacteria MDR and identifying the bacteria that were active against the MDR. Samples were collected from Ternate, Maluku. Isolation of symbiotic bacteria, screening for bacteria which producing secondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria of MDR. Conducting anti-bacterial sensitivity test,  sensitivity test for antibacterial,  DNA exctraction, DNA amplification based on PCR method, DNA sequencing.  Result of 16S r-DNA sequence was then analyzed and edited using GENETYX program and followed by 16S rDNA sequence analysis. Screening of bacteria associated with P. trapezium resulted in 19 isolates with 5 active bacteria. Based on the size of the zone forming and the consistency of zone, so the best isolate is TPT 4.7. The identification shows that TPT 4.7 has a close relationship with the Paracoccus sp. MBIC4019 with homologi of 95%, which shows the relationship at the genus level. Its suggest that these results are very promising as a new antibacterial material. Keywords: antibacterial, symbiotic bacteria, Pleuroploca trapezium, multi drugs resistant

scholarly journals Studies on the Phytochemical Compounds in the Ethanolic Leaf Extract (ELE), Ethanolic Bark Extract (EBE) and Ethanolic Root Extract (ERE) of Bridelia ferruginea Benth (Euphorbiaceae)

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Abdullahi Attah Alfa ◽  
Orukotan Abimbola Ayodeji ◽  
Goji Anthony Donatus Teru ◽  
Kokori Bajeh Tijani
Keyword(s):  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Bioactive Compound ◽  
Fats And Oils ◽  
Bark Extract ◽  
Root Extract ◽  
Suction Pump ◽  
Plant Parts ◽  
Bridelia Ferruginea ◽  
Phytochemical Compounds

The phytochemical compounds of Bridelia ferruginea plant parts was carried out using qualitative method to determine the bioactive compounds present in the plant leave, stem bark and root extracts. The samples was weighed, of which 100 g each of the powder were extracted in solvents (ethanol) 1000 ml macerated and stand for 72 hours. The solvents contained in the maceration bottle was decanted and filtered using a filter paper, the filtration was aided using a suction pump. The filtrate was concentrated using a rotary evaporator and then transferred into thermostatic water cabinet (Temperature was set at 45oC), allowed to dry completely. The plant parts extracts were separately kept in a screw capped bottle for further research. The bioactive compound in the plant parts were detected. The result revealed that Carbohydrates, Saponins, Flavonoids, Tannins, Cardiac Glycosides, fats and oils were present. Alkaloid present in Dragendoff’s test in all plant parts extract but absent in Mayer’s test in only leaf extract. Terpenoids/Steroids present in Liebermann-Burchard’s test in all plant parts extract but absent in Salkowski’s test in only leaf extract. Anthraquinones were absent in all plant parts extracts using Bontrager’s test. Therefore, the presence of these phyto-pharmacological compounds is an indicative that the plant is medicinal and it can be used for the treatment of bacterial and other microbial infections. Further study can be done to separate the individual metabolites to test their antimicrobial activity against some pathogenic bacteria like bacterial meningitis, tuberculosis and syphilis to determine their potency.

Reducing Pathogens by Using Zinc Oxide Nanoparticles and Acetic Acid in Sheep Meat

2014 ◽  
Vol 77 (9) ◽  
pp. 1599-1604 ◽  
Author(s):  
MAHBOUBEH MIRHOSSEINI ◽  
VAHID ARJMAND
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Acetic Acid ◽  
Zno Nanoparticles ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Initial Growth ◽  
Practical Applications ◽  
E Coli ◽  
Sheep Meat

Practical applications of different concentrations (0, 1, 2, 4, 6, and 8 mM) of zinc oxide (ZnO) suspensions containing 1% acetic acid were investigated against the pathogenic bacteria Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. ZnO suspensions (0, 1, 3, 6, and 8 mM) containing acetic acid had a significant inhibitory effect on the growth of L. monocytogenes, E. coli, and S. aureus during 12 h of incubation, and the 8 mM suspensions of ZnO were the most effective against all the strains. These data suggested that the antibacterial activity of ZnO was concentration dependent. Thus, 6 and 8 mM ZnO were selected for further studies in meat. ZnO nanoparticles reduced initial growth of all inoculated strains in meat. To our knowledge, this is the first report describing the antibacterial activity of ZnO nanoparticles in meat and indicates the potential of these nanoparticles as an antibacterial agent in the food industry.

scholarly journals Gut Microbiota Alterations from Three-Strain Yogurt Formulation Treatments in Slow-Transit Constipation

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Xiao-Ran Li ◽  
Chen-Jian Liu ◽  
Xiao-Dan Tang ◽  
He-Ming Zhang ◽  
Yi-Yong Luo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gut Microbiota ◽  
Short Chain Fatty Acid ◽  
Intestinal Bacteria ◽  
Chain Fatty Acid ◽  
Slow Transit Constipation ◽  
Butanoic Acid ◽  
Treatment Groups ◽  
Slow Transit ◽  
Transit Constipation

The objective of this study was to evaluate the effects of a three-strain yogurt formulation in slow-transit constipation (STC) patients. Each individual in both treatment groups consumed 250 mL of the formulated yogurt daily for a week (7 days), and fecal samples were collected for gut microbiota and short-chain fatty acid (SCFA) analyses. A significant increase in the defection frequency (p<0.001) and bacterial diversity (p=0.027) at the 100% sequence homology level and a decrease in the concentrations of acetic acid (p=0.014), propionic acid (p=0.019), and butanoic acid (p=0.005) were observed after the STC patients consumed three-strain yogurt formulation. In addition, the consumption of the three-strain yogurt formulation significantly altered the composition of the intestinal bacteria in the STC patients. The relative abundances of 23 genera in the top dominating genera were altered significantly after the STC patients consumed the yogurt. In summary, the consumption of 250 mL day− the three-strain yogurt formulation described in this study can play a role in improving the symptoms of STC.

Excess Gibbs energy for binary mixtures of acetonitrile with acetic acid, propionic acid, isobutyric acid, and trimethylacetic acid

1991 ◽  
Vol 69 (12) ◽  
pp. 2117-2121 ◽  
Author(s):  
T. S. Banipal ◽  
B. S. Lark ◽  
S. Singh
Keyword(s):  
Acetic Acid ◽  
Gibbs Energy ◽  
Carboxylic Acids ◽  
Propionic Acid ◽  
Binary Mixtures ◽  
Activity Coefficients ◽  
Excess Gibbs Energy ◽  
Composition Range ◽  
Isobutyric Acid ◽  
Dimerization Constant

Total vapour pressures for binary mixtures containing acetic acid, propionic acid, isobutyric acid, and trimethylacetic acid with acetonitrile have been measured for the entire composition range at 298.15 and 318.15 K using a static manometric method. All systems show positive deviations from Raoult's law, enhanced by both an increase in temperature and an increase in the methylation of acetic acid. Activity coefficients have been calculated by taking into consideration the dimerization of these carboxylic acids in the vapour phase. TSE values obtained from GE and earlier reported HE values are found to be negative for acetic acid, about zero for propionic and isobutyric acids, and positive for trimethylacetic acid for the whole composition range. The results have been interpreted in terms of various contributions such as depolymerization, heteromolecular dipole–dipole interactions, and the increasing dimerization constant and steric hindrance with increase of complexity of the acid. Key words: excess Gibbs energy, carboxylic acids, acetonitrile, activity coefficients

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