scholarly journals Efek Seduhan Teh Hitam terhadap Pertumbuhan Candida albicans

2019 ◽  
Vol 2 (1) ◽  
pp. 24-31
Author(s):  
. Felita ◽  
Shelly Lelyana ◽  
Triswaty Winata
Keyword(s):  
Candida Albicans ◽  
Disc Diffusion ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Kandidiasis oral adalah salah satu infeksi fungal yang mengenai mukosa oral, biasanya disebabkan oleh Candida albicans. Candida albicans adalah salah satu spesies Candida yang merupakan flora normal pada membran mukosa rongga mulut dan merupakan jamur yang sering menyebabkan kandidiasis pada rongga mulut dan dapat bersifat patogen opportunistik ketika pertahanan sistemik menurun. Pemanfaatan bahan alam dapat dipilih sebagai salah satu alternatif mencegah infeksi kandidiasis. Teh hitam merupakan salah satu minuman yang memiliki banyak manfaat kesehatan untuk host. Tujuan dari penelitian ini adalah untuk mengetahui efek dari seduhan teh hitam terhadap pertumbuhan Candida albicans. Penelitian ini bersifat eksperimental laboratorik, menggunakan metode disc diffusion dengan melakukan pengukuran zona hambat yang dibentuk oleh seduhan teh hitam konsentrasi 12,5%; 25%; 50%; 100%, kontrol negatif (cakram kosong), dan kontrol positif (cakram nistatin) pada Mueller Hinton Agar yang diinkubasi pada suhu 37? C selama 24 jam menggunakan jangka sorong. Hasil uji ANOVA menyatakan bahwa p < 0,05 sehingga dilanjutkan dengan uji Tukey yang menyatakan bahwa seduhan teh hitam dengan konsentrasi 100%; 50%; 25%; 12,5% tidak memiliki efek terhadap pertumbuhan Candida albicans. Kesimpulan dari penelitian ini adalah seduhan teh hitam tidak memiliki efek terhadap pertumbuhan Candida albicans.

scholarly journals Russia-made Mueller–Hinton agar: compliance with contemporary requirements

2019 ◽  
Vol 9 (2) ◽  
pp. 409-416
Author(s):  
L. V. Domotenko ◽  
I. S. Kosilova ◽  
A. P. Shepelin
Keyword(s):  
Quality Control ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Nutrient Media ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

At present, a rise of antimicrobial resistance requires that susceptibility of infectious agents to antimicrobial agents could be accurately evaluated as related errors may lead to selecting improper therapeutics provoking spread of drug resistance. Pathogen sensitivity to antimicrobial agents is commonly determined by a disc diffusion method. A quality of nutrient medium used in assays plays a crucial role influencing final results. In Russia, it turned out that regulatory documents such as the nationwide guidelines and clinical recommendations outlining methodology for antimicrobial susceptibility testing underlay availability in domestic market few nutrient media, including Mueller–Hinton Agar, AGV medium etc. exhibiting sometimes unsatisfactory quality. To harmonize such methodology with international requirements, theStateResearchCenterfor Applied Microbiology and Biotechnology has developed a technology and promoted manufacture of Russia-made Mueller–Hinton agar satisfying requirements of EUCAST documents, clinical guidelines, and ISO/TS 16782:2016. The main objective of this study was to compare quality of new agar product with five similar foreign media while examining 11 test strains by disc diffusion method. As a result, some of nutrient media available to the Russian market turned out to be off-standard: not all of them satisfy to the EUCAST requirements and clinical guidelines since diameter distribution for growth inhibition recommended by EUCAST for quality control does not fit into permissible range. Moreover, susceptibility of P. aeruginosa ATCC 27853 to aminoglycosides, fluoroquinolones, Meropenem, as well as S. aureus ATSS 25923 and E. faecalis ATCC 29212 to tigecycline was assessed with certain mistakes. The data obtained by us were analyzed in accordance to the new document ISO/TS 16782:2016 “Clinical laboratory testing — criterion for acceptable lots of dehydrated Mueller–Hinton agar and broth for antimicrobial susceptibility testing”, not approved yet In Russia. To determine potential reason for deviation of data from reference range, we measured concentration of bivalent metals in all nutrient media examined by atomic emission spectrometry with inductively coupled plasma. We determined new patterns affecting reliability of results on microbial antibiotic susceptibility. A need to check intralaboratory quality control of nutrient media was emphasized.  

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic

2017 ◽  
Vol 62 (No. 7) ◽  
pp. 410-415 ◽  
Author(s):  
J. Bzdil ◽  
O. Holy ◽  
J. Toporcak
Keyword(s):  
Salmonella Enterica ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
The Czech Republic ◽  
Selective Enrichment ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Paratyphi B ◽  
Standard Disc

The aim of this study was to describe two case studies of reptile disease, in which the primary pathogen was apparently Salmonella enterica subspecies enterica serovar Paratyphi B. Pathological examinations, sampling from different organs and cloacal swabs was performed on a dead savannah monitor (Varanus exanthematicus) and a sick green tree python (Chondropython viridis). This material was subjected to culture examination, including selective enrichment using standard methods. Typing was performed using MALDI-TOF and strains were also serologically typified. The utilisation of d-tartrate was confirmed biochemically and also using PCR. Antibiotic susceptibility was determined by the standard disc diffusion method using Mueller-Hinton agar without blood and antibiotic discs. In both cases the detected Salmonella enterica subspecies enterica serovar Paratyphi B was positive for d-tartrate and exquisitely susceptible to chloramphenicol, tetracycline, ampicillin, amoxycillin/clavulanic acid, gentamicin, ceftazidime, enrofloxacin and piperacillin.

scholarly journals The efficacy of Calendula officinalis tincture as an antibacterial on in vitro Pseudomonas aeruginosa

2002 ◽  
Author(s):  
◽  
Mbuso Mabuza
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Calendula Officinalis ◽  
Disc Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Microbial Study

The aim of this in vitro microbial study was to evaluate the efficacy of Calendula officinalis tincture 60% (v/v) ethanol as an antibacterial on in vitro Pseudomonas aeruginosa. The standardised disc - diffusion method was employed. Seven pairs of Mueller - Hinton agar plates were used.

scholarly journals ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116

2020 ◽  
Vol 19 (3A) ◽  
Author(s):  
Abdullah Abdullah ◽  
Rahmawati Rahmawati ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungal Infections ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Mueller Hinton ◽  
Nocardia Sp ◽  
Mueller Hinton Agar ◽  
Good Concentration

Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of  C. albicans InaCC-Y116.    

scholarly journals Uji Efektivitas Ekstrak Jintan Hitam (Nigella Sativa) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Pada Medium MHA (Mueller Hinton Agar)

2020 ◽  
pp. 1-9
Author(s):  
Armanto Makmun ◽  
Zulfiyah Surdam ◽  
Andi Mufida Gunawan
Keyword(s):  
Staphylococcus Aureus ◽  
Nigella Sativa ◽  
Disc Diffusion ◽  
Mueller Hinton ◽  
Post Test ◽  
Mueller Hinton Agar

Indonesia merupakan salah satu negara berkembang yang menghadapi penyakit infeksi. Staphylococcus aureus merupakan patogen utama pada manusia dan hampir setiap orang pernah mengalami infeksi Staphylococcus aureus yang bervariasi dalam beratnya, mulai dari keracunan makanan hingga infeksi kulit ringan sampai berat yang mengancam jiwa. Namun mengingat tingginya angka resistensi antibiotik maka penggunaan tanaman obat sebagai alternatif terapi merupakan pilihan yang lebih aman. Penelitian Ini Bertujuan Mengetahui khasiat ekstrak jintan hitam dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini adalah penelitian experimental post test dengan menggunakan metode disc diffusion untuk melihat bagaimana efektivitas Ekstrak Jintan Hitam (Nigella sativa) sebagai antimikroba terhadap Staphylococcus aureus secara in vitro. Pada ekstrak jintan hitam didapatkan zona hambat yang paling tinggi pada konsentrasi 100% dengan rerata zona hambat sebesar 24 mm, sedangkan zona hambat yang paling rendah yaitu pada konsentrasi 25% dengan rerata zona hambat sebesar 10 mm.Pada kontrol positif menggunakan antibiotik Kloramfenikol didapatkan rerata zona hambat sebesar 30 mm yakni sensitif menghambat pertumbuhan bakteri, sedangkan pada kontrol negatif menggunakan akuades tidak terbentuk zona hambat. Terbentuk daya hambat terhadap bakteri Staphylococcus aureus dengan pemberian ekstrak jintan hitam pada semua konsentrasi.

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