scholarly journals Features of obtaining metaphase chromosomes Caprovinea

2021 ◽  
pp. 66-70
Author(s):  
Inna Petrovna Novgorodova ◽  
Bailar Sadraddin ogly Iolchiev ◽  
Pavel Mikhailovich Klenovitskiy ◽  
Yuriy Aleksandrovich Prytkov
Keyword(s):  
Animal Species ◽  
Jugular Vein ◽  
Farm Animals ◽  
Optimal Parameters ◽  
Modified Technique ◽  
Metaphase Chromosomes ◽  
Nutrient Media ◽  
Cheap Method ◽  
Species Characteristics ◽  
Short Time

Obtaining cultures of lymphocyte cells for studying of animal karyotypes is an easily accessible, relatively cheap method and allows obtaining results in a short time. We optimized the usual method with corrected  some adjustments to the protocol to obtain metaphase plates of chromosomes of various animal species of good quality. At the same time, such criteria as the use of different media for the cultivation of blood cell cultures and the possibility of using them in different species of animals were taken into account. Blood samples were collected from the jugular vein in goats (n=15), sheep (n=30) and round (n=1). The cell culture was cultivated for 72 h; colchicine was added for 70 h in an amount of 0.5 ?l / ml. The study used nutrient media such as DMEM (Dulbecco?s Modified Eagle?s Medium) и RPMI 1640 (Roswell Park Memorial Institute) and mitogens phytohemagglutinin and Concanavalin A in different concentrations. Optimal parameters for the preparation of metaphase plates with good chromosome distribution were obtained using RPMI 1640 medium supplemented with dry L-glutamine and the simultaneous use of mitogens such as conconavalin and PHA (phytohemagglutinin). The number of plates of good quality when obtaining a culture of lymphocyte cells, depending on the type of animals, ranged from 2.17 to 6.52% in sheep, 33.3-66.7% in goats, and reached 100% in tur. The modified technique made it possible to reduce the loss of cells during the preparation of chromosome preparations. Thus, the results of our study show that the growth and division of lymphocyte cells isolated from the peripheral blood of farm animals depends on the composition of the environment and the species characteristics of the animals.

Registration of animal rabies in Krasnodar region

2020 ◽  
pp. 3-4
Author(s):  
Oleg Yu. Chernykh ◽  
◽  
Vadim A. Bobrov ◽  
Sergey N. Zabashta ◽  
Roman A. Krivonos ◽  
...  
Keyword(s):  
Urban Form ◽  
Animal Species ◽  
Farm Animals ◽  
Natural Focus ◽  
Dead End ◽  
Wide Range ◽  
Epizootic Process ◽  
Krasnodar Region ◽  
Spread Of Infection ◽  
Reporting Data

Rabies remains a constant threat to humanity in many parts of the world. At the same time, scientifically grounded antiepizootic measures should be based on the peculiarities of the regional epizootology of this zooanthroponosis. The authors studied the epizootological and statistical reporting data of the Kropotkin Regional Veterinary Laboratory, presented an analysis of the registration of rabies in animals in Krasnodar region. From the obtained data, it should be noted that despite the wide range of animals involved in the epizootic process of rabies infection in Krasnodar region, dogs, cats and foxes play a major role in the reservation and spread of infection, which account for 78.6. Of the total number of registered cases, 15.5% falls on foxes, that indicates the natural focus of the disease, along with the manifestation of the disease in an urban form. At the same time, stray and neglected dogs and cats, which occupy a significant place among the total number of sick animals, are also sources and spread of the infection. Thus farm animals (8.3% of the total number of infected animals) are a biological dead end for the infection. Isolated cases of the disease were noted in muskrat, donkey, raccoon, raccoon dog, marten, ferret and jackal. The authors also established the specific morbidity of various animal species with rabies infection, that is an important aspect in the development and implementation of antiepizootic measures complex

scholarly journals A modified technique for the production of an arterio-venous shunt in sheep, allowing a comparison of biocompatibility of synthetic materials

1990 ◽  
Vol 24 (1) ◽  
pp. 44-47 ◽  
Author(s):  
W. A. Stertmann ◽  
H. H. Scheld ◽  
J. Kubicek ◽  
H. Fitz ◽  
H. Lukas ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Drug Safety ◽  
Extracorporeal Circulation ◽  
Jugular Vein ◽  
Artificial Kidney ◽  
Modified Technique ◽  
Multiple Administration ◽  
Synthetic Materials ◽  
Venous Shunt ◽  
Clinical Impairment

Polytetrafluorethylene (PTFE) prostheses were implanted in 12 sheep as a shunt between the carotid artery and the jugular vein using an end-to-side anastomosis technique. This technique allows repeated tests of the pharmacological and toxicological safety of artificial kidney units after both single and multiple administration. Further-more, it enables the investigation of detoxification of compounds via dialysis, thus contributing to drug safety. Implantation of the prosthesis was uncomplicated. Connection to the extracorporeal circulation was achieved via catheters and maintained using a pump with an output of up to 300 ml/min. This enabled maintenance of extracorporeal circulation for several hours without clinical impairment to the animals. The AV-shunts remained functional for between 8 and 253 days (mean 112·3 days).

Chromosomal instability in different species of agricultural animals (review)

2021 ◽  
pp. 19-26
Author(s):  
V. A. Andreeva
Keyword(s):  
Chromosomal Instability ◽  
Animal Species ◽  
Western Siberia ◽  
Reproductive Function ◽  
Farm Animals ◽  
Chromosomal Disorders ◽  
Important Indicator ◽  
Karyotypic Analysis ◽  
Different Types ◽  
Almost All

The purpose of the work was to analyze the frequency of chromosomal instability in different species of agricultural animals in Western Siberia on the basis of literature data. The analysis of the literature on the topic of somatic chromosomal instability in agricultural animals has been carried out. Despite the stability of the chromosome set, deviations from it are quite common. It is noteworthy that chromosomal instability is characteristic of almost all individuals in the population and serves as an important indicator for assessing the natural mutability of chromosomes. It has been found if an aberration appeared in one tissue, it is very likely to occur in others. It has been noted that somatic chromosomal instability occurs in animals with reduced reproductive function, as well as those suffering from any pathology. For example, in calves with parakeratosis the increase in the frequency of chromatid and isochromatid breaks has been found. Analysis of the frequency of aberrations in sires, which differ in the level of perinatal mortality of offspring revealed the increased percentage of offspring mortality in fathers with the large number of chromosomal disorders. Therefore, low fertilization, spontaneous abortions and stillbirths may be indications for karyotypic analysis. It has been revealed that the lability of the karyotype is inherent in all animal species, regardless of the species, sex and age, as well as the morphofunctional state. The data on some types of somatic chromosomal instability in different types of farm animals in Western Siberia has been presented. Such indicators as the frequency of polyploidy, the number of cells with fragments of chromosomes, as well as single and paired fragments of chromosomes has been given. Different types of chromosomal instability varied depending on the animal species and climate zone. The presented data can be accepted as a physiological norm and used in veterinary medicine and animal science.

scholarly journals ACTUALITY OF APPLICATION OF BIOGAS INSTALLATIONS IN RUSSIA AND ABROAD

2017 ◽  
Vol 12 (2) ◽  
pp. 71-74
Author(s):  
Булат Зиганшин ◽  
Bulat Ziganshin ◽  
Ильназ Кашапов ◽  
Ilnaz Kashapov ◽  
Ильнур Гайфуллин ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Power Plants ◽  
Biogas Production ◽  
Operating Experience ◽  
Farm Animals ◽  
National Program ◽  
Urban Wastewater ◽  
The World ◽  
Short Time ◽  
Made In

The first scientific developments in the field of biogas technologies were made in Russia more than 70 years ago. Since the 50th years one of the main directions was the anaerobic processing of activated sludge and sediments of urban wastewater. This method attracted attention in connection with the idea of obtaining biogas mainly from the manure of farm animals. Thanks to this in the middle of 50th years a number of pilot plants for biogas production were built in Zaporozhia, Belorussian, Georgian, Moldavian branches of All-Russian Institute of Agriculture Electrification, and also in Ekaterinburg. However, the operating experience of these installations was insignificant - one - two seasons. The problem of obtaining and using biogas is given great attention abroad. In a short time, in many countries around the world a whole industry for the production of biogas has emerged. The leader in the development of biogas industry is China. Since the middle of 1970, the National Program for the production of biogas from livestock wastes has been operating in this country. Currently, there are 10 million farm bioreactors. In addition, 40 000 biogas stations, 24 000 biogas treatment plants operate in China, which provides operation of 190 power plants.

scholarly journals THE PRODUCTION OF SELECTIVE ADDITIVES TO GROWING MEDIUM DRIGALSKI LACTOSE AGAR

2019 ◽  
pp. 95-102
Author(s):  
Владимир Ермаков ◽  
Vlyadimir Ermakov ◽  
Оксана Датченко ◽  
Oksana Datchenko ◽  
Юлия Курлыкова ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
First Generation ◽  
Animal Species ◽  
Agar Medium ◽  
Nutrient Media ◽  
Gram Negative ◽  
Selective Media ◽  
Growing Medium ◽  
Agar Media ◽  
Growth And Reproduction

The purpose of the study is to improve the selective supplement for selective media with the purpose to produce enterobacteria. Tasks of the study are to identify the sensitivity of strains obtained of enterobacteria in regard to an-tibiotics; develop a new selective supplement with antibiotics to the nutrient medium Drigalski Lactose Agar. Media should have a content that in the best way possible ensures the growth and reproduction of microorganisms of cer-tain species or family. Intensive biotechnology development and Microbiology allows today to develop new nutrient media and modify the already existing content of media. The object of the study was a new selective additive with antibiotics to the nutrient medium Drigalski Lactose Agar. 253 isolates of bacteria produced from the intestinal mi-crobiotope of different animal species have been the Material for research. The study was conducted in the period from 2010 to 2017. Carbenicillin 30±2.3 from the group of carboxypenicillins and piperacillin 37±2.5 from the group of ureidopenicillins, kanamycin 24±1.5, amikacin 26±1.7 and gentamicin 25±0,8, cefepime 38±3.2 from the group of IV generation cephalosporins, tetracycline 28±1.6, doxycycline 34±2.3 and chloramphenicol 31±2.5, nalidixic acid 37±2.8, trimethoprim 35±3,4 demonstrated the greatest antimicrobial activity against all cultures of enterobac-teria that has been achieved. The high resistance of enterobacteria was shown to benzylpenicillin from the group of natural penicillins, to streptomycin, cephalotine from the group of cephalosporins of the first generation, to polymyx-in B, to ofloxacin (tarivid) and metronidazole. Antibacterial drugs effective against the accompanying gram-positive and gram-negative microflora were considered as the samples of the selective components. Vancomycin from the group of glycopeptides, linezolid from the group of oxazolidinones, and telithromycin from the group of ketolides were chosen. Antibiotics vancomycin and telithromycin in a dose of 0.008 g/dm3, linezolid 0.004 g/dm3 were cho-sen as the selective additive to Drigalski Lactose Agar medium.

scholarly journals Treatment of cattle pyroplasmidosis in the conditions of Dagestan Republic

2021 ◽  
Vol 344 (5) ◽  
pp. 8-10
Author(s):  
S. Sh. Abdulmagomedov ◽  
A. Yu. Aliev ◽  
R. M. Bakrieva ◽  
E. A. Belkin
Keyword(s):  
Live Weight ◽  
The Body ◽  
Control Measures ◽  
Ixodid Ticks ◽  
Farm Animals ◽  
Blood Parasites ◽  
Control Group ◽  
Single Intramuscular Injection ◽  
Short Time ◽  
And Control

Relevance. Dagestan Republic in terms of natural and climatic characteristics is the most favorable for the of ixodid ticks - carriers of pathogens of blood-parasitic diseases of farm animals. In this regard, further improvement of the set of scientifically grounded control measures and the search for new promising chemotherapeutic drugs of the prevention and treatment of pyroplasmidosis of cattle are major problem of great national economic importance.Materials and methods. The studies were carried out in farms, unfavorable on pyroplasmidosis, in the conditions of Dagestan Republic. The object of the study was cattle, spontaneously invaded by various types of blood parasites. Experеmental and control groups in production experiments were selected according to the principle of analogues. In the first control group (n = 10) the drug was not used. The second (n = 10) was injected with the drug DAC 5% at a dose of 1 ml/20 kg (DV 2.5 mg/kg), intramuscularly, at the rate 5 ml per 100 kg of live weight. The animals of the third (n = 10) were injected with the injectable preparation forticarb 10% at a dose of 4 ml/100 kg (DV 4 mg/kg) of live weight, intramuscularly, once.Results. It was found that with a single intramuscular injection of forticarb at the rate 2 ml/50 kg of live weight, the temperature and parasitic reaction in the body of sick animals decreased in a very short time. Therapeutic efficacy in pyroplasmidosis of cattle was 90%.

scholarly journals Relevance of animal infections in SARS-Cov-2 spread: an update after 1 year of pandemic.

2021 ◽  
Author(s):  
Frazzini Sara ◽  
Amadori Massimo ◽  
Lauretta Turin ◽  
Federica Riva
Keyword(s):  
Animal Species ◽  
Natural Infection ◽  
Farm Animals ◽  
World Health ◽  
Chinese City ◽  
Experimental Infections ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Therapy Studies

In December 2019, several cases of pneumonia caused by a novel Coronavirus, later identified as SARS-CoV-2, were detected in the Chinese city of Wuhan. Due to its rapid, worldwide spread, on 11 March 2020 the World Health Organization declared a pandemic state. Since this new virus is genetically similar to the coronaviruses of bats, it was thought to have a zoonotic origin. Within a year of the appearance of SARS-CoV-2, several cases of infection were also reported in animals, suggesting animal-to-human and animal-to-animal transmission within mammals. Natural infection has been found in both companion and captive animals such as lions, tigers and gorillas. Among farm animals, the only ones found to be susceptible to SARS-CoV-2 infection so far are minks. Experimental infections have documented the susceptibility to SARS-CoV-2 of several animal species, such as humanized mice, hamsters, cats, dogs, ferrets, racoon dogs, cattle and non-human primates. Experimental infections are crucial for both elucidation of the role of animals in transmission and development of appropriate animal models for pathogenesis and therapy studies. This review aims to update the knowledge on natural and experimental SARS-CoV-2 infections in animals.

Qualitative behaviour assessment as an indicator of animal emotional welfare in farm assurance

Livestock ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 180-183
Author(s):  
Richard Cooper ◽  
Françoise Wemelsfelder
Keyword(s):  
Physical Health ◽  
Outcome Measures ◽  
Animal Species ◽  
Good Life ◽  
Farm Animals ◽  
Emotional Wellbeing ◽  
Qualitative Behaviour ◽  
Assessment Techniques ◽  
Potential Benefits ◽  
Sentient Beings

Acknowledgement of animals as sentient beings (including farm animal species), capable of experiencing positive and negative emotions, has highlighted the need for suitable ‘welfare outcome’ measures in farm assurance schemes. Current schemes tend to focus on measures of physical health and productivity, but there is as yet a lack of indicators addressing farmed animals' emotional wellbeing. A number of assessment techniques exist that may help us develop such indicators, and better understand the extent to which farm animals do, or do not, experience ‘a good life’. This article focuses on one such technique, ‘qualitative behaviour assessment’ (QBA), and reviews the potential benefits of, and challenges to, its practical deployment in the field.

scholarly journals Evaluation of HBV-Like Circulation in Wild and Farm Animals from Brazil and Uruguay

2019 ◽  
Vol 16 (15) ◽  
pp. 2679
Author(s):  
Yasmine R. Vieira ◽  
Moyra M. Portilho ◽  
Flávia F. Oliveira ◽  
Alexandro Guterres ◽  
Débora Regina L dos Santos ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Animal Species ◽  
Farm Animals ◽  
Core Antigen ◽  
Qualitative Polymerase Chain Reaction ◽  
Health Response ◽  
B Virus ◽  
Polymerase Chain ◽  
Partial Genome

The origin of the hepatitis B virus is a subject of wide deliberation among researchers. As a result, increasing academic interest has focused on the spread of the virus in different animal species. However, the sources of viral infection for many of these animals are unknown since transmission may occur from animal to animal, human to human, animal to human, and human to animal. The aim of this study was to evaluate hepadnavirus circulation in wild and farm animals (including animals raised under wild or free conditions) from different sites in Brazil and Uruguay using serological and molecular tools. A total of 487 domestic wild and farm animals were screened for hepatitis B virus (HBV) serological markers and tested via quantitative and qualitative polymerase chain reaction (PCR) to detect viral DNA. We report evidence of HBsAg (surface antigen of HBV) and total anti-HBc (HBV core antigen) markers as well as low-copy hepadnavirus DNA among domestic and wild animals. According to our results, which were confirmed by partial genome sequencing, as the proximity between humans and animals increases, the potential for pathogen dispersal also increases. A wider knowledge and understanding of reverse zoonoses should be sought for an effective One Health response.

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