scholarly journals Edible biofilm formation from guava seed waste fermentation

2018 ◽  
Vol 1 ◽  
pp. 00005
Author(s):  
Sari Darmasiwi ◽  
Oktaviana Herawati ◽  
Endah Retnaningrum
Keyword(s):  
Light Microscopy ◽  
Biofilm Formation ◽  
Waste Product ◽  
Glass Slide ◽  
Fermentation Products ◽  
Fermentation Method ◽  
Glass Slides ◽  
Guava Seed ◽  
Banana Leaves ◽  
Seed Fermentation

Guava seed is by-product from the consumption of guava fruits. We interested to explore further the potential of guava seed waste using fermentation method. The purpose of this research was to determine the ability of biofilm formation produced from fermentation of guava seed. Fermented guava seed was prepared by solid-state fermentation method using banana leaves wrap at 37 °C for 72 h. It were then continued with isolation and screening of bacteria from the fermentation products, preparation of bacteria cultures to be used in biofilm formation, and formation of biofilm by glass slides and broth cultures methods. The edible biofilm formation by glass slide method was observed by light microscopy using 0.5 % Crystal Violet dye, while biofilm formation by broth cultures method was observed by transmission electron microscopy (TEM) using phosphotungstic acid 2 % dye. The results show that there were 3 (three) strains lactic acid bacteria (LAB) candidates isolated from fermented guava seed waste product (J6, J7, and J8 strains). The observation by light microscopy showed that J7 strain was the only strain which was unable to form biofilm by glass slide method. All the strains showed the ability to form biofilms in different stages by broth cultures method. Thus, guava seed fermentation was able to produce edible biofilm but the LAB strains still need to be identified further.<br>

Concordance between digital pathology and light microscopy in general surgical pathology: a pilot study of 100 cases

2014 ◽  
Vol 67 (12) ◽  
pp. 1052-1055 ◽  
Author(s):  
Joseph P Houghton ◽  
Aaron J Ervine ◽  
Sarah L Kenny ◽  
Paul J Kelly ◽  
Seamus S Napier ◽  
...  
Keyword(s):  
Pilot Study ◽  
Light Microscopy ◽  
Digital Images ◽  
Digital Pathology ◽  
Glass Slide ◽  
Clinical Consequence ◽  
Histopathological Diagnosis ◽  
Original Diagnosis ◽  
Glass Slides ◽  
Viable Method

Aim(1) A pilot study to determine the accuracy of interpretation of whole slide digital images in a broad range of general histopathology cases of graded complexity. (2) To survey the participating histopathologists with regard to acceptability of digital pathology.Materials and methodsGlass slides of 100 biopsies and minor resections were digitally scanned in their entirety, producing digital slides. These cases had been diagnosed by light microscopy at least 1 year previously and were subsequently reassessed by the original reporting pathologist (who was blinded to their original diagnosis) using digital pathology. The digital pathology-based diagnosis was compared with the original glass slide diagnosis and classified as concordant, slightly discordant (without clinical consequence) or discordant. The participants were surveyed at the end of the study.ResultsThere was concordance between the original light microscopy diagnosis and digital pathology-based diagnosis in 95 of the 100 cases while the remaining 5 cases showed only slight discordance (with no clinical consequence). None of the cases were categorised as discordant. Participants had mixed experiences using digital pathology technology.ConclusionsIn the broad range of cases we examined, digital pathology is a safe and viable method of making a primary histopathological diagnosis.

Thin-Section Preparation and Transmitted-Light Microscopy

2010 ◽  
pp. 115-135
Keyword(s):  
Light Microscopy ◽  
Polymeric Composites ◽  
Glass Slide ◽  
Transmitted Light ◽  
Fiber Reinforced ◽  
Transmitted Light Microscopy ◽  
Ultrathin Sections ◽  
Microscopy Techniques ◽  
Surface Mounting ◽  
Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

Staining methods for sections of epon-embedded tissues for light microscopy

1970 ◽  
Vol 48 (1) ◽  
pp. 189-191 ◽  
Author(s):  
C. Roland Leeson ◽  
Thomas S. Leeson
Keyword(s):  
Light Microscopy ◽  
Synthetic Resin ◽  
Electron Microscopic ◽  
Glass Slides ◽  
Staining Methods

Sections 0.5–2 μ thick are mounted on clean glass slides and allowed to dry. A number of staining procedures are described. After the sections are stained, permanent preparations are made by mounting them in a synthetic resin. The methods result in sections which are suitable for routine light microscopy and for comparison with adjacent electron microscopic sections.

scholarly journals Direct-Plate Serological Grouping of Beta-Hemolytic Streptococci from Primary Isolation Plates with the Phadebact Streptococcus Test

1978 ◽  
Vol 7 (4) ◽  
pp. 356-360
Author(s):  
Malcolm Slifkin ◽  
Carol Engwall ◽  
Gail R. Pouchet
Keyword(s):  
Tween 80 ◽  
Glass Slide ◽  
Correct Identification ◽  
Primary Isolation ◽  
Direct Testing ◽  
Clinical Microbiologist ◽  
Glass Slides ◽  
Precipitin Test

The grouping of beta-hemolytic streptococcal isolates by a new direct-plate procedure employing Phadebact Streptococcus Test reagents was compared with the results obtained with the 4- and 24-h Phadebact grouping procedure and with the Lancefield grouping obtained with a capillary precipitin test. The new procedure employed a modification of the Phadebact procedure that permitted the grouping of streptococci on glass slides with a minimum of five primary isolated colonies. When only five to eight colonies were available for direct testing with each Phadebact reagent, coagglutination was better manifested when the colonies were disaggregated on a glass slide in a loopful of Tween 80 solution. Further enhancement of the coagglutination reaction was effected when the respective Phadebact reagents were employed in relatively small volumes. The direct-plate procedure permitted the correct identification of 127 out of 129 betahemolytic isolates. The 4-h method correctly identified 192 of the 200 streptococci tested. All of the 200 isolates tested by the 24-h procedure and the Lancefield grouping were correctly identified. The direct-plate Phadebact procedure affords the clinical microbiologist a rapid and reliable means of identifying groups A, B, C, and G beta-hemolytic streptococci. When sufficient numbers of primary colonies are not available for the direct procedure, the 4- or 24-h procedures may be employed.

scholarly journals Validation of grading of non-invasive urothelial carcinoma by digital pathology for routine diagnosis

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Richard Colling ◽  
Hayleigh Colling ◽  
Lisa Browning ◽  
Clare Verrill
Keyword(s):  
Urothelial Carcinoma ◽  
Digital Pathology ◽  
Glass Slide ◽  
Low Grade ◽  
Non Invasive ◽  
Routine Diagnosis ◽  
Grade Versus ◽  
Glass Slides ◽  
Urothelial Bladder ◽  
Bladder Carcinomas

Abstract Background Pathological grading of non-invasive urothelial carcinoma has a direct impact upon management. This study evaluates the reproducibility of grading these tumours on glass slides and digital pathology. Methods Forty eight non-invasive urothelial bladder carcinomas were graded by three uropathologists on glass and on a digital platform using the 1973 WHO and 2004 ISUP/WHO systems. Results Consensus grades for glass and digital grading gave Cohen’s kappa scores of 0.78 (2004) and 0.82 (1973). Of 142 decisions made on the key therapeutic borderline of low grade versus high grade urothelial carcinoma (2004) by the three pathologists, 85% were in agreement. For the 1973 grading system, agreement overall was 90%. Conclusions Agreement on grading on glass slide and digital screen assessment is similar or in some cases improved, suggesting at least non-inferiority of DP for grading of non-invasive urothelial carcinoma.

scholarly journals High-sensitivity SERS based sensing on the labeling side of glass slides using low branched gold nanoparticles prepared with surfactant-free synthesis

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34290-34298
Author(s):  
Tuğba Tezcan ◽  
Chia-Hsien Hsu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Glass Slide ◽  
Dopamine Detection ◽  
Glass Slides

High-sensitivity dopamine detection on aggregated low branched nanoparticles on labelling side of glass slide as a SERS based sensor.

scholarly journals Inactivation of the pgmA Gene in Streptococcus mutans Significantly Decreases Biofilm-Associated Antimicrobial Tolerance

2019 ◽  
Vol 7 (9) ◽  
pp. 310 ◽  
Author(s):  
Martin Nilsson ◽  
Michael Givskov ◽  
Svante Twetman ◽  
Tim Tolker-Nielsen
Keyword(s):  
Cell Wall ◽  
Streptococcus Mutans ◽  
Congo Red ◽  
Biofilm Formation ◽  
Cell Wall Composition ◽  
Mutant Library ◽  
Wild Type ◽  
Glass Slides ◽  
Increased Sensitivity ◽  
Wall Components

Screening of a Streptococcus mutans mutant library indicated that pgmA mutants displayed a reduced biofilm-associated tolerance toward gentamicin. The biofilms formed by the S. mutans pgmA mutant also displayed decreased tolerance towards linezolid and vancomycin compared to wild-type biofilms. On the contrary, the resistance of planktonic S. mutans pgmA cells to gentamycin, linezolid, and vancomycin was more similar to wild-type levels. Investigations of biofilms grown in microtiter trays and on submerged glass slides showed that pgmA mutants formed roughly the same amount of biofilm as the wild type, indicating that the reduced antimicrobial tolerance of these mutants is not due to diminished biofilm formation. The pgmA gene product is known to be involved in the synthesis of precursors for cell wall components such as teichoic acids and membrane glycolipids. Accordingly, the S. mutans pgmA mutant showed increased sensitivity to Congo Red, indicating that it has impaired cell wall integrity. A changed cell wall composition of the S. mutans pgmA mutant may play a role in the increased sensitivity of S. mutans pgmA biofilms toward antibiotics.

Cytochemical methods for the rapid demonstration of infection at the tissue-biomaterial interface

1992 ◽  
Vol 50 (1) ◽  
pp. 634-635
Author(s):  
J. Hanker ◽  
J.J. Dobbins ◽  
P.E. Yates ◽  
B.L. Giammara
Keyword(s):  
Light Microscopy ◽  
Culture Techniques ◽  
The Past ◽  
Glass Slides ◽  
Staining Methods ◽  
The Times

Infection can be an extensive problem developing during implantation of an autogenous or prosthetic (biomaterial) device or graft. Although culture techniques are invaluable in identifying the responsible microorganisms, the times required frequently emphasize the need for rapid staining methods which can reduce the classification times from days to hours. Studies in our laboratories over the past few years have resulted in microwave-accelerated stains and in methods developed by Giammara, which enable the rapid study of glass slides and coverslips by electron as well as light microscopy.

In Situ Embedding of Cell Monolayers on Untreated Glass Surfaces for Vertical and Horizontal Ultramicrotomy.

1980 ◽  
Vol 38 ◽  
pp. 644-645
Author(s):  
Kai Chien
Keyword(s):  
Organic Solvent ◽  
Light Microscopy ◽  
Cell Monolayer ◽  
Glass Slide ◽  
Plastic Substrates ◽  
Cell Monolayers ◽  
Glass Surfaces ◽  
In Situ Embedding ◽  
The Ideal

Untreated glass is the ideal supporting substrate for cell cul¬ture growth since it is extremely flat and smooth, transparent and insoluable in organic solvent. However, difficulties have been encountered in removing polymerized epoxy resin from a glass surface following in situ cell monolayer embedment. Vari¬ous techniques have been made to grow cell cultures on either coated glass surfaces or plastic substrates. The purpose of this abstract is to describe a heat separation technique which when used together with a newly designed embedding mold allows cell monolayers to be transferred from untreated coverglass or glass slide to pre-shaped tissue blocks. The resulting tissue block can be easily separated and used directly for orientation light microscopy prior to ultramicrotomy.

Hints On Removing Epon Specimen Blocks From Glass Slides

1998 ◽  
Vol 6 (3) ◽  
pp. 14-17
Author(s):  
Wolfgang Muss
Keyword(s):  
Physical Properties ◽  
Thin Section ◽  
Glass Slide ◽  
Glass Slides

Depending on the quality of the glass slide (not all brands seem to be the same quality and therefore display variable physical properties), post-polymerized specimen blocks can be separated from object slides. These can be of varying area size, and can include a selected area of your former semi-thin section or the whole area (as used in re-embedding techniques).

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