scholarly journals Histopathological changes and immunosuppression induce by diazepam in mice

2014 ◽  
Vol 13 (1) ◽  
pp. 59
Author(s):  
A. H. Ali
Keyword(s):  
Immune Response ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Brain Parenchyma ◽  
Pathological Examination ◽  
Central Vein ◽  
Cell Mediated Immunity ◽  
White Pulp ◽  
Histological Changes ◽  
Immunological Examination

In order to investigate the histological changes and immunosuppression effects of diazepam in mice, forty white mice of both sexes were divided into four groups equally. 1st group was immunized twice with pasturella multocida (bacterines) with two weeks intervals. 2nd group was immunized as in the 1st group and at same time administrated orally with 0.6 mg\kg b.w of diazepam daily for 8 weeks. 3rd group was administrated with diazepam as in the 2nd group while 4th group was served as control negative group. Immunological examination revealed that the diazepam inducing depresses of the both arms of immune response, the cell mediated immunity and humeral immunity as comparing with vaccinated non-treatment animals. The pathological examination revealed that the diazepam induced large multiple granulomatous liver lesions consist form aggregation mononuclear cells particularly macrophages and lymphocytes. In addition, section of kidney showed marked inflammatory cells infiltration particularly mononuclear cells and neutrophils in the interstitial tissues was seen. In spleen there was congestion of blood vessels with mononuclear cells in their lumen and depletion of white pulp as well as proliferation of megakaryocytes, in addition to gliosis in the brain parenchyma was seen. The immunized animals showed mild pathological changes characterized by aggregation mononuclear cells around central vein in addition to proliferation of kupffer cells, Spleen show proliferation of lymphocytes in the periartiriolar sheath as well as protein aqueous materials deposition around white pulp. We concluded that diazepam induced Histological changes in the internal organs of mice and stimulated the immune response diminished its toxic effects.

scholarly journals Toxopathological and immunotoxical effects of thiamethoxam in white mice

2014 ◽  
Vol 13 (1) ◽  
pp. 15
Author(s):  
A.R. Dirwal
Keyword(s):  
Immune Response ◽  
Electron Microscope ◽  
Mononuclear Cells ◽  
Brucella Melitensis ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Pathological Examination ◽  
Interstitial Tissue ◽  
Liver Section ◽  
Subepithelial Layer

In order to investigate toxopathological and immunotoxic, effects of thiamethoxam in mice. Forty eight white mice, both sexes were divided into four groups equally, 1st group was immunized twicely with Brucella melitensis Rev1.with two weeks intervals. 2nd group was immunized as in the 1st group and at same time administrated orally with 83.73mg\kg B.W of thiamethoxam daily for 6 weeks.3rd group was administrated with thiamethoxam as in the 2nd group while the 4th group was served as control negative group. Immunological examination DTH and humoral immunity revealed that the thiamethoxam induced depressed in both arms of immune response as comparing with vaccinated non-treatment animals. The pathological examination revealed that the thiamethoxam induced, necrosis, hypertrophy of hepatocytes with multiple granulomatous lesion scatter in liver parenchyma and these lesions were progressive with period time particularly at 6 weeks post-treatment that showed marked proliferation of hepatocytes. The electron microscope examination to liver section showed lipid droplet, proliferation of mitochondria enlargement in addition to distraction of nuclear membrane and presence facular space, also there was severe hemorrhage in the renal interstitial tissue with inflammatory cells infiltration together with fibrosis of the glomeruli wall. Congestion with inflammatory cells infiltration in the red pulp of spleen, in addition to gliosis in the brain parenchyma was seen .The immunized animals showed mild pathological changes characterized by aggregation of mononuclear cells, The electron microscope to liver section showed normal organelles cell. with lymphoid hyperplasia in the spleen and in subepithelial layer of intestinal mucosa. We concluded that thiamethoxam induced toxopathological changes in the internal organs of mice and stimulated the immune response diminished its toxic effects.

scholarly journals Concurrent helminthosis engendered gastroenteritis in a leopard Panthera pardus

2019 ◽  
Vol 56 (4) ◽  
pp. 323-328
Author(s):  
R. Kumar ◽  
A. D. Moudgil ◽  
A. Sharma ◽  
R. Sharma ◽  
R. Masand ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Toxocara Canis ◽  
Intestinal Content ◽  
Detailed Examination ◽  
Pathological Examination ◽  
Polymorphonuclear Cells ◽  
Panthera Pardus ◽  
Parasitic Gastroenteritis ◽  
Present Case Study

SummaryThe necropsy of a leopard (Panthera pardus), succumbed to a chronic ailment exhibited a mixed parasitic gastroenteritis. Gross internal examination of carcass revealed the presence of round and tapeworms in the stomach and intestines with diffuse catarrhal and hemorrhagic gastroenteritis. The detailed examination of the intestinal content revealed the presence of Toxocara canis and Spirometra species eggs. Also, the gross morphological investigation of round and tapeworms approved the presence of both species. Histo-pathological examination showed sloughing of intestinal epithelium, hemorrhages, and ulcerative areas with the infiltration of polymorphonuclear cells admixed with mononuclear cells. Lungs revealed the accumulation of eosinophilic edematous fl uid in the alveolar spaces along with inflammatory cells. These parasites are pathogenic to precious wild felids and often pose a threat of zoonotic transmission due to spill-over infections. The present case study is an attempt to put on record a case of parasitic gastroenteritis in a captive leopard.

scholarly journals Immune Response to Infection withMycobacterium ulcerans

2001 ◽  
Vol 69 (3) ◽  
pp. 1704-1707 ◽  
Author(s):  
Travis M. Gooding ◽  
Paul D. R. Johnson ◽  
Dianne E. Campbell ◽  
John A. Hayman ◽  
Elizabeth L. Hartland ◽  
...  
Keyword(s):  
Immune Response ◽  
Mononuclear Cells ◽  
Tuberculin Test ◽  
Acid Fast Bacillus ◽  
Mycobacterium Ulcerans ◽  
Cell Mediated Immunity ◽  
Peripheral Blood Mononuclear ◽  
T Cell Anergy ◽  
Skin Ulcers ◽  
Ifn Γ

ABSTRACT Mycobacterium ulcerans is a slow-growing, acid-fast bacillus that causes chronic necrotizing skin ulcers known as Buruli ulcers. Previously reported information on immunity to this mycobacterium is limited. We examined immune responses to M. ulcerans and M. bovis BCG in patients with M. ulcerans disease and in 20 healthy control subjects (10 tuberculin test positive and 10 tuberculin test negative). Cell-mediated immunity was assessed by stimulating peripheral blood mononuclear cells (PBMC) with whole mycobacteria and then measuring PBMC proliferation and the production of gamma interferon (IFN-γ). Humoral immunity was assessed by immunoblotting. PBMC from all subjects showed significantly greater proliferation and IFN-γ production in response to stimulation with living mycobacteria compared with killed cells. However, PBMC from subjects with past or current M. ulcerans disease showed significantly reduced proliferation and production of IFN-γ in response to stimulation with live M. ulcerans or M. bovis than PBMC from healthy, tuberculin test-positive subjects (P < 0.001) and showed results in these assays comparable to those of tuberculin test-negative subjects (P > 0.2). Serum from 9 of 11 patients with M. ulcerans disease, but no control subject, contained antibodies to M. ulcerans. The results indicate that patients with M. ulcerans infection mount an immune response to M. ulcerans as evidenced by antibody production, but they demonstrate profound systemic T-cell anergy to mycobacterial antigens. These findings may explain some of the distinct clinical and pathological features of M. ulcerans-induced disease.

scholarly journals The effectiveness of Pectin in the reduction of histological changes caused by exposure to monosodium glutamate in female mice: مدى فعالية البكتين في الحد من التغيرات النسيجية التي يسببها تناول جلوتامات أحادي الصوديوم على إناث الفئران

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Sarah Ibrahim Al Othman, Faten khalif Alanazi, Ghada Jaber S
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Monosodium Glutamate ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Control Group ◽  
Central Vein ◽  
Histological Changes ◽  
Female Mice ◽  
Liver And Kidney

Monosodium glutamate (MSG) is widely used as a food additive. Excessive consumption of monosodium glutamate has also been shown to affect the liver and kidneys, causing damage to these tissues because of oxidative stress leading to increased production of reactive oxygen species (ROS). The purpose of the study described in this paper was to find out how the liver and kidney toxicity caused by monosodium glutamate can be mitigated using pectin. To this end, 30 albino mice females were divided into four groups. The animals were distributed in special cages. 12-15 weeks with an average body weight of 60 grams. The animals were divided into four groups: the experimental control group (1) comprising 5 female mice were given normal drinking water and the treated group (2) comprising 10 female mice were given monosodium glutamate at a dose of 3 g/kg body weight in drinking water. For three weeks, the treatment group (3) comprising 10 female mice was given pectin at a dose of 300 mg/70 kg body weight in drinking water immediately after the monosodium glutamate dose for three weeks and the pectin group (4) comprising 5 female mice were given Pectin at a dose of 300 mg/70 kg body weight in drinking water for three weeks. The mice were then anesthetized, dissected, and liver and kidney samples were taken from female mice and kept in a 10% neutral formalin solution to make tissue segments. The results showed many histological changes in the liver, such as congestion of the central vein, widening of the sinuses, and the appearance of signs of the death of most hepatocytes, infiltration of the central vein and an invasion of inflammatory cells around the central vein with the emergence of several gaps within the cells. Many of them cavity with the death of most of the tubule cells, the closure of some of them and the expansion and infiltration in others and bleeding inside the tissue. Pectin therapy has led to the disappearance of most of these changes and the emergence of a clear improvement in hepatic and renal tissue.

scholarly journals Immunocytochemical identification and quantitation of the mononuclear cells in the cerebrospinal fluid, meninges, and brain during acute viral meningoencephalitis.

1984 ◽  
Vol 159 (1) ◽  
pp. 77-88 ◽  
Author(s):  
T R Moench ◽  
D E Griffin
Keyword(s):  
T Cells ◽  
Cerebrospinal Fluid ◽  
B Cells ◽  
Sindbis Virus ◽  
Time Course ◽  
Mononuclear Cells ◽  
Inflammatory Responses ◽  
Inflammatory Cells ◽  
Brain Parenchyma ◽  
Immunoperoxidase Staining

The mononuclear cells of the central nervous system (CNS) inflammatory response were characterized in cerebrospinal fluid (CSF), meningeal exudate, and brain parenchyma of mice 3-14 d after infection with Sindbis virus. The inflammatory infiltrate in CSF peaked and resolved before that of the parenchyma or meningeal exudate. Immunoperoxidase staining with monoclonal antibodies identified CSF inflammatory cells to be almost exclusively T cells, while inflammatory cells in the brain parenchymal perivascular cuffs and the meninges were a mixture of T cells, B cells, and macrophages. The percentage of B cells and macrophages increased at the later time points. Approximately 20% of CSF and 50% of the cells present early in the perivascular cuffs were not identified, suggesting that another subset of inflammatory cells may be present. We concluded that significant differences exist in the time course and cellular composition of the inflammatory responses in different compartments of the CNS during an acute viral infection.

scholarly journals Pathology of Fowl Typhoid and Molecular Detection of its Pathogen

2019 ◽  
Vol 23 (2) ◽  
pp. 49-60
Author(s):  
J Hosen ◽  
MM Rahman ◽  
J Alam ◽  
ZC Das ◽  
MAHNA Khan ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Lymphoid Cells ◽  
Central Vein ◽  
White Pulp ◽  
Cell Hyperplasia ◽  
Salmonella Gallinarum ◽  
Fowl Typhoid ◽  
Hematoxylin And Eosin ◽  
Neutral Formalin ◽  
Human And Animal Diseases

Salmonellae are important group of pathogens responsible for human and animal diseases. The present study was undertaken with the aim to study pathology of fowl typhoid caused by Salmonella entarica subsp. enteric serovar Gallinarum and to identify Salmonella serovars by polymerase chain reaction (PCR) based molecular method isolated from commercial layer, broiler and sonali chickens of Gazipur district, Bangladesh. A total of 150 cloacal, intestinal and liver swab samples were collected in sterie nutrient and tetrathionate broth from apparently healthy, sick and dead chicken the necropsy. Organ samples were collected in 10% buffered neutral formalin. The collected tissues were fixed, processed, sectioned, stained with hematoxylin and eosin (H&E) and examined at low and high power microscopic fields. Grossly, the liver appeared larger and hemorrhagic with focal necrosis. Catarrhal inflammation on intestinal mucosa was seen. The ova were deformed, discolored and cystic. Microscopically, focal necroses with the infiltration of mononuclear cells were seen with congestion of the central vein. Spleen showed severe depletion of lymphoid cells in white pulp along with reticuloendothelial cell hyperplasia. The section of ovary showed deformed ova with hemorrhages. Samples were subjected to various cultural, biochemical, and molecular examinations and the prevalence was identified 28% cases. Isolated bacteria appeared gram (-)ve, and arranged in short chain. PCR was performed targeting invA gene of Salmonella Gallinarum and amplified 184-bp fragment of the isolates confirmed specific infectivity. Ann. Bangladesh Agric. (2019) 23(2) : 49-60

Cellular immune response in intraventricular experimental neurocysticercosis

Parasitology ◽  
2015 ◽  
Vol 143 (3) ◽  
pp. 334-342 ◽  
Author(s):  
VANIA B. L. MOURA ◽  
SARAH B. LIMA ◽  
HIDELBERTO MATOS-SILVA ◽  
MARINA C. VINAUD ◽  
PATRICIA R. A. N. LOYOLA ◽  
...  
Keyword(s):  
Immune Response ◽  
Mononuclear Cells ◽  
Late Phase ◽  
Parasitic Infection ◽  
Inflammatory Cells ◽  
Histopathological Analysis ◽  
Systemic Immune Response ◽  
Local Inflammatory Response ◽  
Immune Profile

SUMMARYNeurocysticercosis (NCC) is considered a neglected parasitic infection of the human central nervous system. Its pathogenesis is due to the host immune response, stage of evolution and location of the parasite. The aim of this study was to evaluate thein situand systemic immune response through cytokines dosage (IL-4, IL-10, IL-17 and IFN-γ) as well as the local inflammatory response of the experimental NCC withTaenia crassiceps. Thein situand systemic cellular and inflammatory immune response were evaluated through the cytokines quantification at 7, 30, 60 and 90 days after inoculation and histopathological analysis. All cysticerci were found within the cerebral ventricles. There was a discrete intensity of inflammatory cells of mixed immune profile, polymorphonuclear and mononuclear cells, at the beginning of the infection and predominance of mononuclear cells at the end. The systemic immune response showed a significant increase in all the analysed cytokines and predominance of the Th2 immune profile cytokines at the end of the infection. These results indicate that the location of the cysticerci may lead to ventriculomegaly. The acute phase of the infection showed a mixed Th1/Th17 profile accompanied by high levels of IL-10 while the late phase showed a Th2 immune profile.

scholarly journals Using Modified Anthraxin And Its Evaluation For Testing Cell Mediated Immunity And Studying Histopathological Changes In Lab Animals

2007 ◽  
Vol 31 (2) ◽  
pp. 93-103
Author(s):  
An' am J.L. Al-Jeboury
Keyword(s):  
Bacillus Anthracis ◽  
Blood Vessels ◽  
Guinea Pigs ◽  
Booster Dose ◽  
Skin Testing ◽  
Inflammatory Cells ◽  
Cell Mediated Immunity ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Potential Use

This study aims to apply a new procedure in preparation of anthraxin fromcell wall extracts of a virulent Bacillus anthracis strain 34F2 (Sterne).Anthraxin was evaluated for potential use as skin testing agent to measurecell-mediated immunity. Guinea pigs were immunized and boostedsubcutaneously with Sterne live veterinary anthrax vaccine. Two weeks afterthe booster dose, animals were skin tested with the antigen which recorded15.6mm as a mean of erythema and 2.5 mm difference of thickness ascompared to the control. Histological changes corresponded with the delayedtype of hypersensitivity (DTH) in which the changes involvedinfiltration of inflammatory cells particularly macrophages, neutrophiles andeosinophils in the skin dermis and in perivascular region in addition toaccumulation of fluid (edema) in the dermis and congestion of blood vessels.

scholarly journals Profiles of Peripheral Immune Cells of Uncomplicated COVID-19 Cases with Distinct Viral RNA Shedding Periods

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 514
Author(s):  
Denise Utami Putri ◽  
Cheng-Hui Wang ◽  
Po-Chun Tseng ◽  
Wen-Sen Lee ◽  
Fu-Lun Chen ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Cells ◽  
Mononuclear Cells ◽  
Immune Cell ◽  
Severe Disease ◽  
Viral Rna ◽  
Disease Course ◽  
Peripheral Blood Mononuclear ◽  
Peripheral Immune Cells ◽  
Cell Profile

The heterogeneity of immune response to COVID-19 has been reported to correlate with disease severity and prognosis. While so, how the immune response progress along the period of viral RNA-shedding (VRS), which determines the infectiousness of disease, is yet to be elucidated. We aim to exhaustively evaluate the peripheral immune cells to expose the interplay of the immune system in uncomplicated COVID-19 cases with different VRS periods and dynamic changes of the immune cell profile in the prolonged cases. We prospectively recruited four uncomplicated COVID-19 patients and four healthy controls (HCs) and evaluated the immune cell profile throughout the disease course. Peripheral blood mononuclear cells (PBMCs) were collected and submitted to a multi-panel flowcytometric assay. CD19+-B cells were upregulated, while CD4, CD8, and NK cells were downregulated in prolonged VRS patients. Additionally, the pro-inflammatory-Th1 population showed downregulation, followed by improvement along the disease course, while the immunoregulatory cells showed upregulation with subsequent decline. COVID-19 patients with longer VRS expressed an immune profile comparable to those with severe disease, although they remained clinically stable. Further studies of immune signature in a larger cohort are warranted.

scholarly journals Anti-inflammatory genes in PBMCs post-spontaneous intracerebral hemorrhage

2021 ◽  
Vol 12 (1) ◽  
pp. 58-66
Author(s):  
Doan Nguyen ◽  
Vi Tran ◽  
Alireza Shirazian ◽  
Cruz Velasco-Gonzalez ◽  
Ifeanyi Iwuchukwu
Keyword(s):  
Intracerebral Hemorrhage ◽  
Negative Correlation ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Favorable Outcome ◽  
Hospital Length Of Stay ◽  
Spontaneous Intracerebral Hemorrhage ◽  
Hematoma Volume ◽  
Peripheral Blood Mononuclear ◽  
Anti Inflammatory

Abstract Background Neuroinflammation is important in the pathophysiology of spontaneous intracerebral hemorrhage (ICH) and peripheral inflammatory cells play a role in the clinical evolution and outcome. Methodology Blood samples from ICH patients (n = 20) were collected at admission for 5 consecutive days for peripheral blood mononuclear cells (PBMCs). Frozen PBMCs were used for real-time PCR using Taqman probes (NFKB1, SOD1, PPARG, IL10, NFE2L2, and REL) and normalized to GAPDH. Data on hospital length of stay and modified Rankin score (MRS) were collected with 90-day MRS ≤ 3 as favorable outcome. Statistical analysis of clinical characteristics to temporal gene expression from early to delayed timepoints was compared for MRS groups (favorable vs unfavorable) and hematoma volume. Principle findings and results IL10, SOD1, and REL expression were significantly higher at delayed timepoints in PBMCs of ICH patients with favorable outcome. PPARG and REL increased between timepoints in patients with favorable outcome. NFKB1 expression was not sustained, but significantly decreased from higher levels at early onset in patients with unfavorable outcome. IL10 expression showed a negative correlation in patients with high hematoma volume (>30 mL). Conclusions and significance Anti-inflammatory, pro-survival regulators were highly expressed at delayed time points in ICH patients with a favorable outcome, and IL10 expression showed a negative correlation to high hematoma volume.

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