scholarly journals Pathology of Fowl Typhoid and Molecular Detection of its Pathogen

2019 ◽  
Vol 23 (2) ◽  
pp. 49-60
Author(s):  
J Hosen ◽  
MM Rahman ◽  
J Alam ◽  
ZC Das ◽  
MAHNA Khan ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Lymphoid Cells ◽  
Central Vein ◽  
White Pulp ◽  
Cell Hyperplasia ◽  
Salmonella Gallinarum ◽  
Fowl Typhoid ◽  
Hematoxylin And Eosin ◽  
Neutral Formalin ◽  
Human And Animal Diseases

Salmonellae are important group of pathogens responsible for human and animal diseases. The present study was undertaken with the aim to study pathology of fowl typhoid caused by Salmonella entarica subsp. enteric serovar Gallinarum and to identify Salmonella serovars by polymerase chain reaction (PCR) based molecular method isolated from commercial layer, broiler and sonali chickens of Gazipur district, Bangladesh. A total of 150 cloacal, intestinal and liver swab samples were collected in sterie nutrient and tetrathionate broth from apparently healthy, sick and dead chicken the necropsy. Organ samples were collected in 10% buffered neutral formalin. The collected tissues were fixed, processed, sectioned, stained with hematoxylin and eosin (H&E) and examined at low and high power microscopic fields. Grossly, the liver appeared larger and hemorrhagic with focal necrosis. Catarrhal inflammation on intestinal mucosa was seen. The ova were deformed, discolored and cystic. Microscopically, focal necroses with the infiltration of mononuclear cells were seen with congestion of the central vein. Spleen showed severe depletion of lymphoid cells in white pulp along with reticuloendothelial cell hyperplasia. The section of ovary showed deformed ova with hemorrhages. Samples were subjected to various cultural, biochemical, and molecular examinations and the prevalence was identified 28% cases. Isolated bacteria appeared gram (-)ve, and arranged in short chain. PCR was performed targeting invA gene of Salmonella Gallinarum and amplified 184-bp fragment of the isolates confirmed specific infectivity. Ann. Bangladesh Agric. (2019) 23(2) : 49-60

scholarly journals Histopathological changes and immunosuppression induce by diazepam in mice

2014 ◽  
Vol 13 (1) ◽  
pp. 59
Author(s):  
A. H. Ali
Keyword(s):  
Immune Response ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Brain Parenchyma ◽  
Pathological Examination ◽  
Central Vein ◽  
Cell Mediated Immunity ◽  
White Pulp ◽  
Histological Changes ◽  
Immunological Examination

In order to investigate the histological changes and immunosuppression effects of diazepam in mice, forty white mice of both sexes were divided into four groups equally. 1st group was immunized twice with pasturella multocida (bacterines) with two weeks intervals. 2nd group was immunized as in the 1st group and at same time administrated orally with 0.6 mg\kg b.w of diazepam daily for 8 weeks. 3rd group was administrated with diazepam as in the 2nd group while 4th group was served as control negative group. Immunological examination revealed that the diazepam inducing depresses of the both arms of immune response, the cell mediated immunity and humeral immunity as comparing with vaccinated non-treatment animals. The pathological examination revealed that the diazepam induced large multiple granulomatous liver lesions consist form aggregation mononuclear cells particularly macrophages and lymphocytes. In addition, section of kidney showed marked inflammatory cells infiltration particularly mononuclear cells and neutrophils in the interstitial tissues was seen. In spleen there was congestion of blood vessels with mononuclear cells in their lumen and depletion of white pulp as well as proliferation of megakaryocytes, in addition to gliosis in the brain parenchyma was seen. The immunized animals showed mild pathological changes characterized by aggregation mononuclear cells around central vein in addition to proliferation of kupffer cells, Spleen show proliferation of lymphocytes in the periartiriolar sheath as well as protein aqueous materials deposition around white pulp. We concluded that diazepam induced Histological changes in the internal organs of mice and stimulated the immune response diminished its toxic effects.

scholarly journals Morphological and Quantitative Characteristics of the Skin of Holstein Cattle in the Amur Region

2018 ◽  
Vol 7 (3.5) ◽  
pp. 78
Author(s):  
Мarinа E. Ostyakova ◽  
Irina Yu. Sayapina ◽  
Nikolay Nikolay M. Mandro ◽  
Natal'ya V. Trush ◽  
Yuriy A. Gavrilov ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Structural Features ◽  
High Reactivity ◽  
Amur Region ◽  
Elastic Fibers ◽  
Holstein Cattle ◽  
Holstein Breed ◽  
Hematoxylin And Eosin ◽  
Neutral Formalin

The skin of mammals has a high reactivity to the conditions of the external and internal environment. There is no information about the features of the structure of the skin of Holstein cattle in the context of its adaptation to the sharply continental with the symptoms of monsoon climate in the available literature. The purpose of the study was to determine the structural features of the skin of the sternal and costal regions of cattle of the Holstein breed in the Amur region. For the study, the skin of clinically healthy mature Holstein cows was taken. Time of research - autumn. The skin was fixed in a 10% aqueous solution of neutral formalin. Paraffin sections with a thickness of 5-7 μm were prepared. The sections were stained with hematoxylin and eosin, collagen fibers were detected by the Van Gison method, elastic fibers by Weygert. A distinctive feature of the papillary layer of the dermis was a well-developed system of venous sinuses that anastomosed with each other. The abundance of mononuclear cells in the lumen of the sinuses indicated the important role of the skin as an organ of the immune system that provides adaptation and protection of animals in a sharply continental climate. 

scholarly journals Avian Salmonella Infection: Isolation and Identification of Organisms and Histopathological Study

1970 ◽  
Vol 4 (1) ◽  
pp. 7-12 ◽  
Author(s):  
MS Hossain ◽  
EH Chowdhury ◽  
MM Islam ◽  
MG Haider ◽  
MM Hossain
Keyword(s):  
Causative Agent ◽  
Mononuclear Cells ◽  
Plasma Cells ◽  
Biochemical Properties ◽  
Histopathological Study ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Salmonella Gallinarum ◽  
Focal Necrosis ◽  
Fowl Typhoid

The Present study was performed in the Department of Pathology, BAU, Mymensingh during the period from January to December 2004. The study was conducted to determine the occurrence and pathology of pullorum disease, fowl typhoid and salmonellosis (paratyphoid infection) in dead chickens at necropsy in Mymensingh district of Bangladesh. A total of 70 samples (liver, ovary and intestine) were collected for isolation of bacteria in different media, and identification was performed based on the staining, cultural and biochemical properties of Salmonella spp. Routine histopathological method was used for the detection of tissue level alterations in Salmonella infected cases. Grossly, in all the infected cases the liver was enlarged and congested and in few cases, liver discoloration with focal necrosis. Old raised hemorrhages in the caecal tonsil and congested deformed ova were other important findings. There was catarrhal inflammation in the intestine. Microscopically, the section of livers showed congestion, hemorrhages, focal necrosis with infiltration of mononuclear cells. The pulmonary lesions consisted of sero-fibrinous exudation with mononuclear cell infiltration. The intestinal mucosa exhibited congestion, hemorrhages and infiltration of plasma cells, heterophils and macrophages. Out of 70 samples, 8 isolates were identified as Salmonella (11.42 %). Of them, five isolates were identified as Salmonella gallinarum, causative agent of fowl typhoid, one isolate was characterized as Salmonella pullorum, causative agent of pullorum disease and other two motile salmonella were identified as paratyphoid infection. Keywords: Fowl typhoid, pullorum disease, paratyphoid, histopathology, isolation, identification doi:10.3329/bjvm.v4i1.1518 Bangl. J. Vet. Med. (2006). 4 (1): 07-12

scholarly journals Chitosan-Coated Gold Nanoparticles Induce Low Cytotoxicity and Low ROS Production in Primary Leucocytes, Independent of Their Proliferative Status

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 942
Author(s):  
Helen Yarimet Lorenzo-Anota ◽  
Diana G. Zarate-Triviño ◽  
Jorge Alberto Uribe-Echeverría ◽  
Andrea Ávila-Ávila ◽  
José Raúl Rangel-López ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Death ◽  
Cancer Cells ◽  
Cell Lines ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Lymphoid Cells ◽  
Ros Production ◽  
Biomedical Sciences ◽  
Peripheral Blood Mononuclear

(1) Background: Chitosan-coated gold nanoparticles (CH-AuNPs) have important theranostic applications in biomedical sciences, including cancer research. However, although cell cytotoxicity has been studied in cancerous cells, little is known about their effect in proliferating primary leukocytes. Here, we assessed the effect of CH-AuNPs and the implication of ROS on non-cancerous endothelial and fibroblast cell lines and in proliferative lymphoid cells. (2) Methods: The Turkevich method was used to synthetize gold nanoparticles. We tested cell viability, cell death, ROS production, and cell cycle in primary lymphoid cells, compared with non-cancer and cancer cell lines. Concanavalin A (ConA) or lipopolysaccharide (LPS) were used to induce proliferation on lymphoid cells. (3) Results: CH-AuNPs presented high cytotoxicity and ROS production against cancer cells compared to non-cancer cells; they also induced a different pattern of ROS production in peripheral blood mononuclear cells (PBMCs). No significant cell-death difference was found in PBMCs, splenic mononuclear cells, and bone marrow cells (BMC) with or without a proliferative stimuli. (4) Conclusions: Taken together, our results highlight the selectivity of CH-AuNPs to cancer cells, discarding a consistent cytotoxicity upon proliferative cells including endothelial, fibroblast, and lymphoid cells, and suggest their application in cancer treatment without affecting immune cells.

scholarly journals Specific adsorption of HTLV-I to various target human and animal cells

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1303-1311 ◽  
Author(s):  
K Krichbaum-Stenger ◽  
BJ Poiesz ◽  
P Keller ◽  
G Ehrlich ◽  
J Gavalchin ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mononuclear Cells ◽  
Cultured Cells ◽  
Interleukin 2 ◽  
Flow Cytometric Analysis ◽  
Lymphoid Cells ◽  
Core Antigen ◽  
Target Cells ◽  
Animal Cells ◽  
Homologous Virus

In this report, we describe a flow cytometric analysis of HTLV-I specific binding to fresh and cultured cells on a single cell basis. This assay uses rhodamine hydrocarbon tagged, purified HTLV-I virions according to the procedure originally described for avian retroviruses. Successful HTLV-I transmission was detected by analysis of integrated HTLV-I DNA, virion-associated reverse transcriptase, and/or intracellular HTLV-I core antigen p19 expression. Only a specific virus- cell interaction was detected because nonrhodamine-tagged homologous virus or related HTLV-II interfered with tagged HTLV-I binding. In contrast, an unrelated, nonlabeled animal retrovirus was unable to block tagged HTLV binding. Of the cell lines tested, 2 nonlymphoid mammalian and 3 human lymphoid bound significantly high to moderate levels of HTLV-I-tagged virions. The other three human lymphocyte cell lines were insensitive to HTLV-I adsorption. A direct correlation was observed between HTLV-I binding sites and infectivity of human lymphoid cells alone and not other nonlymphoid animal cells. Fresh normal human mononuclear cells bound low levels of HTLV-I virions. As expected, T lymphocytes demonstrated more binding than did the non-T cell population. Enhancement of HTLV-I cell binding in a subpopulation of mononuclear target cells was achieved with phytohemagglutinin (PHA) activation and interleukin 2 (IL2) stimulation, which correlates well with previously published infectivity studies.

scholarly journals Liposome/gold hybrid nanoparticle encoded with CoQ10 (LGNP-CoQ10) suppressed rheumatoid arthritis via STAT3/Th17 targeting

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241080
Author(s):  
Jooyeon Jhun ◽  
Jeonghyeon Moon ◽  
Jaeyoon Ryu ◽  
Yonghee Shin ◽  
Seangyoun Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Mononuclear Cells ◽  
Therapeutic Potential ◽  
Human Peripheral Blood ◽  
Treated Group ◽  
Hybrid Nanoparticles ◽  
Peripheral Blood Mononuclear ◽  
Hematoxylin And Eosin ◽  
Anti Inflammatory ◽  
Safranin O

Coenzyme Q10 (CoQ10), also known as ubiquinone, is a fat-soluble antioxidant. Although CoQ10 has not been approved as medication by the Food and Drug Administration, it is widely used in dietary supplements. Some studies have shown that CoQ10 has anti-inflammatory effects on various autoimmune disorders. In this study, we investigated the anti-inflammatory effects of liposome/gold hybrid nanoparticles encoded with CoQ10 (LGNP-CoQ10). Both CoQ10 and LGNP-CoQ10 were administered orally to mice with collagen-induced arthritis (CIA) for 10 weeks. The inflammation pathology of joint tissues of CIA mice was then analyzed using hematoxylin and eosin and Safranin O staining, as well as immunohistochemistry analysis. We obtained immunofluorescence staining images of spleen tissues using confocal microscopy. We found that pro-inflammatory cytokines were significantly decreased in LGNP-CoQ10 injected mice. Th17 cell and phosphorylated STAT3-expressed cell populations were also decreased in LGNP-CoQ10 injected mice. When human peripheral blood mononuclear cells (PBMCs) were treated with CoQ10 and LGNP-CoQ10, the IL-17 expression of PBMCs in the LGNP-CoQ10-treated group was significantly reduced. Together, these results suggest that LGNP-CoQ10 has therapeutic potential for the treatment of rheumatoid arthritis.

scholarly journals Atypical hodgkin and reed-sternberg cells in peripheral blood of a patient with advanced stage of Hodgkin's disease - a case report

2003 ◽  
Vol 131 (9-10) ◽  
pp. 400-402 ◽  
Author(s):  
Rajko Milosevic ◽  
Milica Colovic ◽  
Vesna Cemerikic-Martinovic ◽  
Natasa Colovic ◽  
Marina Bogunovic
Keyword(s):  
Bone Marrow ◽  
Lymph Nodes ◽  
Peripheral Blood ◽  
Hodgkin's Disease ◽  
Mononuclear Cells ◽  
Hodgkin’S Disease ◽  
Lymphoid Cells ◽  
High Dose ◽  
Advanced Stage ◽  
Neck Lymph Nodes

The occurrence of abnormal Hodgkin's and Reed-Sternberg cells in the peripheral blood in a patient suffering from Hodgkin's disease has been noticed exceptionally rare in a previous period, and especially rare in last ten years primarily due to successfull treatment of this disease. The presence of atypical mononuclear cells in peripheral blood which cytomorphologically resembled Reed-Sternberg cells was registered in 8 patients till 1966. During the last decade, the presence of atypical mononuclear cells in the peripheral blood was used for their isolation cultivation, and detailed immunophenotypic and genetic analysis. The analysis of mononuclear cells in rare patients with Hodgkin's disease was established that they belong to the B-lymphoid cells with expression of CD30 and CD15 antigens. The examination of presence of Hodgkin's cells in the peripheral blood of patients with Hodgkin's disease is important for patients with advanced stage of the disease in which autologous stem cell transplantation and high dose chmeotherapy is planned. The authors present a 33-year-old patient, who noticed enlarged neck lymph nodes in September 2000, high temperature and loss in weight. On physical examination enlarged neck lymph nodes 5x8 cm and hepatosplenomegaly were found. There was anemia and thrombo-cytopenia, and normal WBC count with 24% of lymphoid elements in differential formula. On histologic examination of lymph nodes Hodgkin?s disease, type nodular sclerosis with mixed cellularity was found. Histology of bone marrow showed nodal lymphomatous infiltration. Immunohistochemistry with monoclonal antibodies of concentrate of peripheral blood cells showed expression of CD30+ and CD15+, immunophenotypically and morphologically matching Reed-Sternberg cells. Cytogentic analysis of mononuclear cells of the bone marrow showed normal karyotype. The patient was in clinical stage IV/V of the disease and chemotherapy with 9 cycles of ABVD+Mp protocol was applied. He is still in remission.

Histomorphometric Analysis of Pre- and Post-Denosumab–Treated Giant Cell Tumor of Bone

2020 ◽  
Vol 28 (8) ◽  
pp. 859-867
Author(s):  
Nasir Ud Din ◽  
Masood Umer ◽  
Yong-Koo Park
Keyword(s):  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Mononuclear Cells ◽  
Giant Cells ◽  
Cell Tumor ◽  
P Value ◽  
Osteoblastic Activity ◽  
Woven Bone ◽  
Hematoxylin And Eosin

Context. Denosumab is a monoclonal antibody against RANK ligand. Its administration in giant cell tumor of bone (GCTB) cases results in elimination of giant cells and new bone formation. Neoplastic stromal cells of GCTB harbor mutation of histone 3.3 and have pre-osteoblastic properties and thus express SATB2. Objectives. To (1) analyze histological changes in post-denosumab–treated GCTB, (2) analyze expression of H3.3G34W and SATB2 in pre- and post-denosumab–treated samples, and (3) to discuss why changes occur in the expression of not only H3.3G34W but also SATB2. Materials and Methods. Hematoxylin and eosin slides of 19 cases of denosumab-treated GCTB were reviewed. Immunohistochemical stains H3.3G34W and SATB2 were performed. The number of positive mononuclear cells were counted and graded. Results. Complete absence of osteoclast-like giant cells (OCLGCs) was noted in most cases along with a fibro-osseous component merging with peripheral shell of reactive bone. Irregular trabeculae of woven bone and osteoid with focal osteoblastic rimming was seen. Spindle cells were arranged predominantly in fascicular pattern. Morphometric analysis of H3.3G34W showed a mean of 68.8% positive stromal cells in pretreatment and a mean of 26.9% positive stromal cells in posttreated specimens with a statistically significant P value (.001). Mean percentage of SATB2-positive stromal cells in the pre- and posttreatment specimens was 36.46% and 20.8%, respectively. Conclusions. Our study validates that denosumab treatment results in marked reduction of OCLGCs with increased osteoblastic activity. Decreased expression of H3.3G34W in posttreatment may be a result of decreased antigenicity of neoplastic mononuclear cells. No significant change in SATB2 expression was noted.

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