Analisis Uji Toksisitas Akut Logam Cu Terhadap Artemia salina dan Daphnia magna

Water pollution can become dangerous if consumed. River could be polluted with some materials namely heavy metal of chopper (Cu). Heavy metals that are accidentally consumed by human body through the skin surface, some through the respiratory or digestive tract and then accumulate in the body over time. To make an early detection of heavy metal and water quality in water bodies, some animals or plants were used. Several types of crustaceans such as Artemia salina and Daphnia magna or commonly referred to as bio indicators. This study analyze comparison of acute toxicity test that represented by LC50 value of Cu in Artemia salina and Daphnia magna. From the research result it was found that Artemia salina and Daphnia magna gave a toxic effect with LC50 values of 11.78 ppm and 7.87 ppm, respectively.

Download Full-text

Pengaruh Konsentrasi Kadmium Terhadap Perubahan warna Dan Persentase Jenis Kelamin Jantan Anakan Daphnia magna

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v1i1.11697 ◽

2019 ◽

Vol 1 (1) ◽

pp. 43

Author(s):

Alfi Hermawati W. S., Rahayu Kusdarwati, Setyawati Sigit, A. Shofy Mubarak

Keyword(s):

Water Pollution ◽

Water Quality ◽

Heavy Metal ◽

Daphnia Magna ◽

Significant Influence ◽

Cadmium Concentration ◽

Colour Changes ◽

Male Sex ◽

Ph Range ◽

Cadmium Target

Abstract Cadmium is one of dangerous heavy metal and it can cause water pollution. As an alternative early warning toward heavy metal in water, Daphnia magna can be used as biology indicator water pollution that caused by heavy metal. Daphnia magna is organism that ussualy used for bioassay in many progressing countries, because that organism have important role in freshwater ecology, short life cycle about 3 weeks and sensitive toward chemical environment. Grade of toxicity heavy metal can measured with lethal and sub lethal parameter. In Daphnia magna, sub lethal parameter that can be observe are colour changes and male sex percentage neonates. The purpose of this research was to know the colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. Target of this research is to gets information about level colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. The main parameter was colour changes and male sex percentage neonates and the secondary parameters were water quality (temperature, pH, dissolved oxygen and NH3) The result of research of giving different cadmium concentration to the adult female Daphnia magna not showing significant influence, nevertheless it is showing significant influence toward male sex percentage neonates Daphnia magna. The treatment with cadmium concentration 0,0004 mg/l produce 100% male neonates Daphnia magna. Water quality during research were in optimal conditions to support Daphnia magna life, those are temperature 26°C, pH range from 8,0 – 8,2 and dissolve oxygen (DO) range from 8,5 – 9,0 mg/l and ammonia 0 – 0,03 mg/l.

Download Full-text

Uji Toksisitas Akut Kulit Kentang (Solanum tuberosum L.) Terhadap Larva Artemia salina Leach Dengan Metode BSLT (Brine Shrimp Lethality Test)

THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST ◽

10.30651/jmlt.v2i2.3090 ◽

2019 ◽

Vol 2 (2) ◽

pp. 10

Author(s):

Baterun Kunsah

Keyword(s):

Brine Shrimp ◽

Solanum Tuberosum L ◽

Artemia Salina ◽

The Body ◽

Control Group ◽

Brine Shrimp Lethality ◽

Advantages And Disadvantages ◽

Lc50 Value ◽

Body Tissues ◽

Potato Skin

Tanggal Submit:28 Januari 2019 Tanggal Review:30 April 2019 Tanggal Publish Online:17 Mei 2019 Potatoes are one of the vegetables that contain substances that are important for the formation of body tissues, such as protein and fat. Potatoes also form red blood cells or hemoglobin (Ca, P and Fe). In Indonesia tend to process potatoes using only meat. The skin is removed, even though the nutritional content of the skin is five times greater than the meat. Potato peels that supply querestin, antioxidants and flavonoids that act as free radical acceptors. (Free radicals are reactive molecules that cause damage to the body that can lead to diseases such as heart disease and cancer) in the skin of potatoes found also effective antioxidants, chlorogenic acid. Potato skin is also proven to increase hemoglobin levels (Khomsan, A, 2009). However, that does not mean potato skin does not have adverse side effects, if the use is not appropriate. In order to use it optimally, you need to know enough information about the advantages and disadvantages and the possibility of abuse. However, the level of toxicity from potato skin is still unknown. Brine Shrimp Lethality Test (BSLT) is one of the oxicity test methods that is widely used in tracing bioactive compounds that are toxic from natural materials. This research is an experimental research with post test-only control group design approach. Treatment with giving potato skin juice to Artemia salina Leach larvae with the aim to determine the potential of plant biological activity based on the toxicity of secondary metabolites contained in it, and at the same time as the initial screening test of anticancer activity of chemical compounds in potato skin juice. The results showed the LC50 value was at a concentration of 0.5% to 1% and based on the results of SPSS 21 analysis with probit analysis obtained LC50 price data of 0.746 gr / ml (7460 μgr / ml), based on the results of research and data analysis it can be concluded that potato skin has an LC50 value at a concentration of 0.746 gr / ml (7460 µgr / ml). With an LC50 price of more than 1000 µg / ml according to the BST method the potato skin does not have the potential for toxicity so the skin of the potato has the potential as an alternative to increasing hemoglobin levels.Keywords: potato skin, toxicity, BSlT

Download Full-text

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Download Full-text

Analysis of Lead Heavy Metal (Pb) Contamination on Matte Lipstick Preparations for Sale in Banda Aceh City Aceh Market

Serambi Journal of Agricultural Technology ◽

10.32672/sjat.v2i1.2053 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Rizki Andalia ◽

Mulia Aria Suzanni ◽

Rini Rini

Keyword(s):

Heavy Metal ◽

Atomic Absorption ◽

Atomic Absorption Spectrophotometry ◽

The Body ◽

Permissible Limit ◽

The Sun ◽

Absorption Spectrophotometry ◽

Pb Contamination ◽

Body Organ ◽

Banda Aceh

Lipstick or lip dye is a cosmetic dose that serves to coloring, decorative the lips, as a moisturizing material and protect the lips from exposure the sun to provide optimum results. Lipstick should not contain chemicals such as lead (Pb) because the Pb is a heavy metal that is very dangerous when continuously used on the skin, because it will be absorbed into the blood and attack the body organ causing the onset of disease. According to BPOM that the lead rate on the lipstick does not exceed the permissible limit of 20 mg/kg or 20 ppm.This research aims to know the levels contained in the samples are 4 brands of matte lipstick that are sold in the Aceh market in Banda Aceh City with the method of atomic absorption spectrophotometry (AAS). The results showed that on the 4 brands of lipstick matte contain heavy metal lead (Pb) with a rate still qualified allowed by BPOM is samples A, B, C, and D, respectively at 0.24 ppm; 0.10 ppm; 2.87 ppm and 1.32 ppm, so that the 4 brands of lipstick matte are still used.

Download Full-text

Assessment of heavy metal river Ingulets surface water pollution

Collection of Scientific Publications NUS ◽

10.15589/jnn20160318 ◽

2017 ◽

Vol 0 (3) ◽

Author(s):

Ganna G. Trokhymenko ◽

Nina V. Tsyhanyuk

Keyword(s):

Water Pollution ◽

Heavy Metal ◽

Surface Water ◽

Surface Water Pollution

Download Full-text

The prevalence of underweight in 9–10-year-old schoolchildren in Liverpool: 1998–2006

Public Health Nutrition ◽

10.1017/s136898000800311x ◽

2009 ◽

Vol 12 (7) ◽

pp. 953-956 ◽

Cited By ~ 13

Author(s):

Lynne M Boddy ◽

Allan F Hackett ◽

Gareth Stratton

Keyword(s):

Mass Spectrum ◽

Body Mass ◽

The Body ◽

Fitness Testing ◽

Policy Makers ◽

Substantial Problem ◽

Prevalence Of Obesity ◽

Grade 3 ◽

The City ◽

Over Time

AbstractObjectiveTo estimate the prevalence of underweight between 1998 and 2006 in Liverpool schoolchildren aged 9–10 years using recently published underweight cut-off points.Design and settingStature and body mass data collected at the LiverpoolSportsLinx project’s fitness testing sessions were used to calculate BMI.SubjectsData were available on 26 782 (n13 637 boys, 13 145 girls) participants.ResultsOverall underweight declined in boys from 10·3 % in 1998–1999 to 6·9 % in 2005–2006, and all sub-classifications of underweight declined, in particular grade 3 underweight, with the most recent prevalence being 0·1 %. In girls, the prevalence of underweight declined from 10·8 % in 1998–1999 to 7·5 % in 2005–2006. The prevalence of all grades of underweight was higher in girls than in boys. Underweight showed a fluctuating pattern across all grades over time for boys and girls, and overall prevalence in 2005–2006 represents over 200 children across the city.ConclusionsUnderweight may have reduced slightly from baseline, but remains a substantial problem in Liverpool, with the prevalence of overall underweight being relatively similar to the prevalence of obesity. The present study highlights the requirement for policy makers and funders to consider both ends of the body mass spectrum when fixing priorities in child health.

Download Full-text

Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

Analytica ◽

10.3390/analytica2030008 ◽

2021 ◽

Vol 2 (3) ◽

pp. 66-75

Author(s):

Toshiki Horikoshi ◽

Chihiro Kitaoka ◽

Yosuke Fujii ◽

Takashi Asano ◽

Jiawei Xu ◽

...

Keyword(s):

Mass Spectrometry ◽

Glucuronic Acid ◽

Laser Desorption ◽

The Body ◽

Time Dependent ◽

Laser Desorption Ionization ◽

Fingerprint Analysis ◽

Desorption Ionization ◽

Acid Conjugate ◽

Over Time

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]+ peak started later than that of [AAP+H]+ peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

Download Full-text