EFFECT OF INJECTABLE COMBINATION ESTROGEN-PROGESTERON AND PROGESTERON-ONLY CONTRACEPTIVES ON GLUCOSE AND MALONDIALALDEHID SERUM

Injectable hormonal contraception has been the most favorable contraception method in Indonesia in 2013 and its number reaches 4,128,115 acceptors (48.56%). The estrogen and progesterone contents in injectable contraception could cause stress, increased appetite, decreased leptin level and insulin resistance, eventually leading to increased blood glucose and serum malondialaldehyde levels. This study aims to observe the main effect of use of combined estrogen-progesteron and only progesteron injectable hormonal contraceptions on glucose level in blood and malondialaldehyde (MDA) level in serum. This is an experimental laboratory study. It uses Randomized pre-post test group research design for glucose level and randomized post test with control for malondialaldehyde level. The data are analyzed using Anova. The significance value is p<0.01. 28 samples are divided into 7 groups and each group consists of 4 samples, namely one control group and 6 treatment groups consisting of P1 combined dose 0.54 mg, P2 combined dose 1.08 mg, P3 combined dose 1.62 mg, P4 DMPA dose 2.7 mg, P5 DMPA dose 5.4 mg, P6 DMPA dose 8.1 mg. Only DMPA contraception at 0.54 mg dose influences the blood glucose level increase by p = 0.00. Only DMPA contraception at 8.1 mg dose does not influence serum MDA level by p = 0.1. The conclusion is DMPA contraception has greater potential of causing blood glucose level increase than the combined contraception. Combined and DMPA contraceptions share the risk of increasing serum MDA level.

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Pharmacodynamics Drug Interactions of Metformin with Aspirin and Nifedipine

Asian Journal of Pharmaceutical Research and Health Care ◽

10.18311/ajprhc/2016/683 ◽

2016 ◽

Vol 8 (1) ◽

pp. 4 ◽

Cited By ~ 1

Author(s):

Khidir A. M. Hassan ◽

Mahmoud M. E. Mudawi ◽

Mansour I. Sulaiman

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Diabetic Control ◽

Serum Glucose ◽

Diabetic Rats ◽

Control Group ◽

Citrate Buffer ◽

Treatment Groups ◽

Diabetic Control Group

Metformin is now being recognized as the standard therapy in T2D patients who are overweight. Metformin has many drug-disease interactions that can increase the risk of metformin-associated lactic acidosis. Therefore this study was conducted to evaluate any possible pharmacodynamic interactions between metformin and drugs used to treat chronic diseases e.g. Hypertension. The rats were fasted overnight before inducing diabetes with streptozotocin. The rats were given an intraperitoneal injection of streptozotocin (50 mg kg−1) freshly prepared in 0.1M sodium citrate buffer. The diabetic state was confirmed 72 h after streptozotocin injection. Diabetic rats were grouped into seven groups each group of five rats and distributed among the normal control group diabetic control group and the treatment groups. The treatment continued for 10 days. Blood samples were taken before treatment and after 10 days and analyzed for serum glucose, cholesterol, HDL, LDL, and triglycerides. In the diabetic control group which was given STZ alone the blood glucose level decreased significantly (p < 0.05) after 10 days but still above the hyperglycemic level (200mg/dl). The same was observed in the group treated with metformin. The group treated with nifedipine and aspirin showed significant reduction (p < 0.01) in the glucose level below the hyperglycemic level (200mg/dl). While the groups treated with (Metformin + Nifedipine) and (Metformin +Aspirin) showed highly significant reduction (P<0.001) in blood glucose level. These results conclude that the combination of (metformin +Nifedipine) and the combination of (Metformin + Aspirin) have highly significant hypoglycemic effect. It also showed that Nifedipine has promising role in reducing blood glucose level, lipid profile especially LDL-cholesterol, and body weight.

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Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090103 ◽

2011 ◽

Vol 9 (1) ◽

pp. 12-16

Author(s):

RIZKY OCKTARINI ◽

DIDING HERI PRASETYO ◽

IPOP SJARIFAH

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

T Test ◽

Herbal Extract ◽

Control Group ◽

Buffer Solution ◽

Control Group Design ◽

Post Test

Ocktarini R, Prasetyo DH, Sjarifah I. 2011. Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin. Biofarmasi 9: 12-16. This study was conducted to determine the effect of herbal extract of anting-anting (Acalypha australis L.) on the decrease of blood glucose level of Balb/C mice induced with Streptozotocin. This research was an experimental laboratory with pre and post-test control group design, conducted in Laboratory of Biochemistry and Histology, Faculty of Medicine, Sebelas Maret University, Surakarta. The test animals used were 16 male mice, about 4-6 weeks of age with approximately 20-30 grams induced with Streptozotocin in 0.02 M citric buffer solution, and a dose of 65 mg/kg body weight. Furthermore, the mice were divided into two groups (Metformin in a dose of 1.3 mg/mice/day and herbal extract of anting-anting 1000 mg/kg BB/day) randomly, each group consisted of 8 mice. All mice were observed for GDS level after 14 days treatment period. The data obtained was processed by using the Independent-Samples T-test statistical test (unpaired t-test) with SPSS for Microsoft Windows Release 18.0. Mean of decreased GDS levels on Metformin group vs herbal extract of anting-anting (145.87 mg/dl vs 144.62 mg/dl, p=0.965). The treatment of herbal extract of anting-anting in a dose of 1000 mg/kg body weight/day could decrease blood glucose level of Balb/C mice induced with Streptozotocin that equal with Metformin.

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Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

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Effect of Zishen Jiangtang Pill, a Chinese Herbal Product, on Rats with Diabetic Osteoporosis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/7201914 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Huilin Li ◽

Shufang Chu ◽

Hengxia Zhao ◽

Deliang Liu ◽

Xuemei Liu ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Metabolic Disorders ◽

Herbal Product ◽

Control Group ◽

Treatment Groups ◽

Chinese Herbal ◽

Tracp 5B ◽

Diabetic Osteoporosis

Diabetic osteoporosis (DO) is a complication of diabetes. Zishen Jiangtang Pill (ZJP) is a Chinese herbal product which has been used in clinic to maintain blood glucose level and bone density for decades. However, the evidence about its mechanism on diabetes and osteoporosis is still unknown. The aim of this study is to investigate therapeutic effect of ZJP on DO in streptozotocin- (STZ-) induced rats. Rats were randomly assigned to 4 groups: one control group (CON), one model group (MOD), and two ZJP treatment groups (1.5 and 3.0 g/kg/d). All rats were treated for 8 weeks. Results showed that ZJP decreased the blood glucose level during OGTT and prevented the changes of FBG and Fins. Similarly, ZJP inhibited the changes of BCa, P, TRACP-5b, CTX-1, BALP, and BGP and the reduction of BMD. In parallel, 1H-NMR metabolomic studies showed that ZJP significantly altered the metabolic fingerprints of blood and urine level. These findings suggest that ZJP can effectively improve glucose metabolism, abnormal bone metabolism, and metabolic disorders in DO rats, which may be a useful alternative medicine for DO therapy.

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Anti-diabetic activity of diphenhydramine in diabetic rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3102 ◽

2020 ◽

Vol 11 (4) ◽

pp. 5067-5070

Author(s):

Pang Jyh Chayng ◽

Nurul Ain ◽

Kaswandi Md Ambia ◽

Rahim Md Noah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Insulin Level ◽

Diabetic Rats ◽

Group Iv ◽

Diabetic Rat ◽

Control Group ◽

Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p<0.05) and group IV (11.34 ±3.67, p<0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p<0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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EFFECTIVENESS OF AMLA JUICE IN REDUCING BLOOD GLUCOSE LEVEL AMONG PATIENTS WITH TYPE II DIABETES IN A SELECTED AREA OF KANYAKUMARI DISTRICT

THE RESEARCH RESERVOIR ◽

10.47211/trr.2021.v07i01.012 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Mrs. Vanitha. S s ◽

Dr. Pramjit kaur

Keyword(s):

Blood Glucose ◽

Standard Deviation ◽

Blood Glucose Level ◽

Glucose Level ◽

Type Ii Diabetes ◽

Control Group ◽

Type Ii ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Experimental Group

Challenges in lifestyle, such as increasesin energy intake and decreasesin physical activity are causing overweight and obesity leading to epidemic increases in type II Diabetes Mellitus. The research approach used for this study was evaluative approach and the research design was true experimental design. 60 patients with type II diabetes, 30 in experimental group and 30 in control group were selected for this study by using purposive sampling technique. Data was collected with the help of self-structured interview schedule. Descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (chi-square, paired ‘t’ test) were used to analyse the data and to test the hypotheses. In the experimental group,the pre-test mean score was 2.966, mean percentage was 59% and standard deviation was 1.129 and in post-testmean score was 2.533, mean percentage was 50.66% and standard deviation was 1.074 with effectiveness of 8.34% and paired‘t’ test value of t=3.971,which was statistically significant (p<0.05) which is an evidence ofthe effectiveness of Amla juice in reducing blood glucose level. Comparison of blood glucose levels in experimental and control groups, shows that the value is statistically highly significant, as was observed from the unpaired ‘t’ test value of 13.39 with P value of <0.05, which is an evidence indicatingthe effect of Amla juice in reducing postprandial blood glucose levels. The resultsfound that the administration of Amla juice did have aneffect in reducing blood glucose level in the experimental group. By comparing the findings of pre-test and post test between the experimental group and the control group,the effect was identified (assessed). The study concluded that the Amlajuice is effective in reducing blood glucose level.

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Urine Glucose Levels Are Disordered Before Blood Glucose Level Increase Was Observed in Zucker Diabetic Fatty Rats

Urine ◽

10.1007/978-981-13-9109-5_8 ◽

2019 ◽

pp. 75-82

Author(s):

Wei Yin ◽

Weiwei Qin

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Urine Glucose ◽

Glucose Levels ◽

Level Increase ◽

Zucker Diabetic Fatty Rats

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ANTI-HYPERGLYCEMIC EFFECT OF DIFFERENT FRACTIONS OF ANNONA RETICULATA LEAF

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13710 ◽

2016 ◽

pp. 256

Author(s):

Soumya Prakash Rout ◽

Durga Madhab Kar ◽

Laxmidhar Maharana

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Ethyl Acetate ◽

Glucose Level ◽

Ethyl Acetate Fraction ◽

Diabetic Control ◽

Control Group ◽

Alcoholic Extract ◽

Diabetic Control Group ◽

Annona Reticulata

ABSTRACT Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae) an interesting plant for investigating its anti-hyperglycemic potential. Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on Streptozotocin (STZ) induced hyperglycemic rats. Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential. Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level. Results: The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is significant (p<0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from 417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL. Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate certain secondary parameters associated with STZ induced hyperglycemia. Conclusion: This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic property in the tested experimental models and should be investigated further. Keywords: Streptozotocin, Diabetes, Dyslipidemia.

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UJI EFEKTIFITAS SINBIOTIK KEFIR TEPUNG PISANG BATU TERHADAP KADAR GLUKOSA DARAH DAN KADAR PROFIL LIPID TIKUS MODEL SINDROM METABOLIK Eﬀectiveness Test of banana (Musa balbisiana) Flour Kefir Synbiotic on Blood Glucose Level and Lipid Profile Level in Metabolic Syndrome Rats Model

Media Gizi Indonesia ◽

10.20473/mgi.v16i3.296-305 ◽

2021 ◽

Vol 16 (3) ◽

pp. 296

Author(s):

Taufik Maryusman ◽

Christine Diane Dien ◽

Santi Herlina Mail

Keyword(s):

Metabolic Syndrome ◽

Blood Glucose ◽

Blood Glucose Level ◽

Lipid Profile ◽

Glucose Level ◽

Intervention Group ◽

Control Group ◽

Musa Balbisiana ◽

Group I ◽

Bonferroni Test

Metabolic Syndrome is a metabolic disorder characterized by hyperglycemia, hypertriglyceridemia, and dyslipidemia which can be repaired through synbiotics. This study is to analyzed the eﬀects of banana (Musa balbisiana) flour kefir synbiotic on blood glucose level and lipid profile level of Metabolic Syndrome rats. This is true experimental study with pre-posttest with control goup design of 24 male Sprague Dawley rats which were divided into 4 groups randomly. Negative control goup (K-) given standard food only, positive control group (K +) given standard food with High Fat Fructose Diet (HFFD), intervention group I (PI) and intervention group II (PII) were given standard food with HFFD and banana (Musa balbisiana) flour kefir synbiotic 1,8 ml/200 g weight/day (PI) and 3,6 ml/200 g weight/day (PII) for three weeks. Blood glucose level was analyzed using glucose oxidase-peroxidase amino antipyrine method, cholesterol level was analyzed using cholesterol oxidase-peroxidase amino antipyrine, and triglyceride level was analyze using glycerol 3 phosphate oxidase phenol amino phenazone method. Result of Analysis of Varian test shows significant diﬀerences in mean between group (p=0,000) followed by PostHoc Bonferroni test or Post Hoc Games-Howel test which showed a mean diﬀerence in the PI and PII groups. Banana (Musa balbisiana) flour kefir synbiotic can reduce blood glucose level and improve lipid profile level.

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Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

Jurnal Kebidanan dan Kesehatan Tradisional ◽

10.37341/jkkt.v3i1.65 ◽

2018 ◽

Vol 3 (1) ◽

Author(s):

Dwi Retna Prihati

Keyword(s):

Body Weight ◽

Test Group ◽

Laboratory Research ◽

Control Group ◽

Female Rat ◽

Wistar Strain ◽

Significant Difference ◽

Post Test ◽

Experimental Laboratory ◽

Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

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