scholarly journals PERTUMBUHAN ISOLAT BAL ASAL BEKATUL DAN PROBIOTIK KOMERSIAL (Lactobacillus acidophilus dan Lactobacillus casei) PADA MEDIA BEKATUL DAN SUSU SKIM

2014 ◽  
Vol 1 (1) ◽  
pp. 27
Author(s):  
Elok Zubaidah ◽  
Erryana Martati ◽  
Ampu Marojahan Resmanto
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Bran ◽  
Skim Milk ◽  
Short Chain Fatty Acids ◽  
Salmonella Typhi ◽  
Total Acid

This research was aimed to study the influence of rice bran and skim milk fermentation media on the growth of lactic acid bacteria and their ability in fermenting complex carbohydrates into short chain fatty acids (SCFA). Indigenous lactic acid bacteria (LAB) were isolated from rice bran and commercial probiotic separately and used for fermenting rice bran and skim milk media. Randomized block design was used with 2 factors i.e. fermenting media type and LAB type. The results showed that fermenting rice bran gave significant effect on the LAB growth, indicated by total LAB cell count, total acid concentration, pH and antibacterial activity. The best treatment was J2-B with total LAB count 1.01 ´ 1010 cfu/mL, total acid 1.14%, pH 3.88 and clear zone diameters against Staphylococcus aureus 13.04 mm, Listeria monocytogenes 12.88 mm, Escherichia coli 12.83 mm and Salmonella typhi 12.53 mm. LAB fermenting rice bran for 48 hours produced lactic acid and SCFA. The highest concentrations of lactic acid (122.1313 mM), acetic acid (10.503 mM), and butyric acid (1.56 mM) were produced by fermentation using LAB J2, L. acidophilus, and L. casei isolate, respectively; whereas the highest propionic acid concentration (6,07 mM) was produced by control fermentation.Keywords: Probiotic, indigenous isolate, rice bran, SCFA, skimmed milk ABSTRAKPeneltian ini bertujuan untuk mengetahui pengaruh dedak dan skim milk sebagai media fermentasi bakteri asam laktat, dan kemampuannya mengubah sumber karbon komplek dedak menjadi asam lemak rantai pendek (short chain fatty acids, SCFA). Bakteri asam laktat lokal diisolasi dari dedak dan probiotik. Desain percobaan adalah acak kelompok dengan 2 faktor, yaitu jenis media fermentasi dan jenis bakteri asam laktat. Hasil penelitian menunjukkan bahwa media fermentasi dengan menggunakan dedak menunjukkan pengaruh yang signifikan terhadap pertumbuhan bakteri yang ditunjukkan dari total sel bakteri asam laktat, total asam yang dihasilkan, pH dan aktivitas antibakteri. Fermentasi dengan menggunakan isolat J2-B menghasilkan total bakteri asam laktat 1,01 ´ 1010 cfu/mL, total asam 1,14%, pH 3,88 dan zona hambatan dengan bakteri uji Staphylococcus aureus 13,04 mm, Listeria monocytogenes 12,88 mm, Escherichia coli 12,83 mm dan Salmonella typhi 12,53 mm. Proses fermentasi bakteri asam laktat menggunakan media dedak selama 48 jam mampu menghasilkan asam laktat dan SCFA. Konsentrasi tertinggi asam laktat (122,13 mM), asam asetat (10,50 mM), dan asam butirat (1,56 mM) masing-masing dihasilkan oleh fermentasi menggunakan BAL J2, isolat L. acidophilus, dan isolat L. casei; sedangkan konsentrasi tertinggi asam propionat (6,07 mM) dihasilkan oleh fermentasi kontrol.Kata kunci: Probiotik, isolat lokal, dedak, SCFA, susu skim

scholarly journals Antibacterial activity of the bacteriocins producing- lactic acid bacteria isolated from some processed meat products against selected indicator bacterial strains

2021 ◽  
Vol 12 (2) ◽  
pp. 640-645
Author(s):  
Huda M Selman ◽  
Ahmed A Mahdi ◽  
Nagat A El Rofaei ◽  
Elnasri M Mutwali
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Meat Products ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Processed Meat ◽  
Bacterial Strains ◽  
Central Public Health

In this study, a total of 25 Lactic Acid Bacteria (LAB) isolates from 15 samples [4 burgers, 4frankfurters, 3 pastramis, 2 sausages and 2baby faeces] were screened for their ability to produce inhibitory substances against three microorganisms which were isolated from meat products: (Salmonella typhi, Staphylococcus aureus and Escherichia coli). Other indicators (reference target organisms) were obtained from the Central Public Health Laboratory, Khartoum State, Sudan. These were: Salmonella typhi ATCC14023, Staphylococcus aureus ATCC29213 and Escherichia coli ATCC25922. The antagonistic activities of the isolates were screened by the direct spot-on-lawn method and well- diffusion method. A total of 25 isolates of LAB were obtained, 15 of which produced promising inhibition zones against all or some of the indicator bacterial strains.

scholarly journals CHARACTERIZATION OF LACTIC ACID BACTERIA AND ANTIMICROBIAL ACTIVITY IN SUI WU’U FROM BAJAWA DISTRICT, NUSA TENGGARA TIMUR, INDONESIA

2020 ◽  
pp. 44-49
Author(s):  
ROSALINA YULIANA AYEN ◽  
ENDANG KUSDIYANTINI ◽  
SRI PUJIYANTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rrna ◽  
Diffusion Method ◽  
E Coli ◽  
Query Cover

Objective: This research aimed to isolate, determine the characteristics of lactic acid bacteria (LAB) of Sui Wu’u from Bajawa, Nusa Tenggara Timur and identify LAB using 16S rRNA potential as antimicrobial activity against pathogenic bacteria. Methods: Sui Wu’u which has been stored for 6 months was obtained from Bajawa district, inoculated on de Man Rogosa-Sharpe Agar (Merck) + 0.5% CaCO3, purification of LAB, characterization of selected isolates, biochemical test, tolerance test for pH, viability to test temperature, and content NaCl, determination of antimicrobial action by the agar well disk diffusion method using antibiotic (Amoxicillin) as a control and as indicator bacteria (Staphylococcus aureus and Escherichia coli) and isolation of genomic 16S rRNA; molecular identification. Results: Based on research results obtained five isolates of LAB, Gram staining the LAB isolated from Sui Wu’u showed that the isolated bacteria (bacilli and coccus) are Gram-positive, catalase-negative and the isolates have tolerance of viability at temperatures of 10°C, 45°C, and 50°C and to salinitas of 4% and 6.5%. The inhibitory zone LAB isolates (2PKT) against E. coli bacteria (20 mm) and S. aureus (12 mm), and (2PKB) against E. coli bacteria (17 mm) and S. aureus (10 mm). The two selected isolates were identified as Lactobacillus fermentum strain HB bacteria with 100% identification value and 98.93% query cover and L. fermentum strain HT with 100% identification value and 99.23% query cover. Conclusion: L. fermentum from Sui Wu’u has antibacterial activity against Staphylococcus aureus and Escherichia coli.

scholarly journals Determination of Antimicrobial Effects of Probiotic Lactic Acid Bacteria and Garlic Extract Against Some Foodborn Pathogenic Bacteria

2017 ◽  
Vol 5 (2) ◽  
pp. 125 ◽  
Author(s):  
Selin Kalkan ◽  
Elçin Taş ◽  
Zerrin Erginkaya ◽  
Emel Ünal Turhan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Meat Product ◽  
Starter Cultures ◽  
Garlic Extract ◽  
Resistant Bacteria

In this study, it was investigated that the inhibition effect of some lactic acid bacteria (Lactobacillus acidophilus NCC68, Lactobacillus casei Shirota, Lactobacillus rhamnosus (Ezal, commercial starter cultures)) which possessed with probiotic characteristics, against Bacillus cereus, Salmonella Enteritidis, Escherichia coli, Escherichia coli 0157:H7 ATCC 35150 and Staphylococcus aureus ATCC 25923. Besides, the inhibitory effect of probiotic cultures which used with meat and meat product additives that garlic extract over the antagonistic effects of sensitive pathogens were investigated in vitro. Consequently, the whole of lactic acid bacteria and garlic extract which were used in this study, showed inhibition effects against all selected pathogenic bacteria. Staphylococcus aureus ATCC 25923 was determined as the most sensitive pathogenic bacteria while Bacillus cereus was the most resistant bacteria against lactic acid bacteria and garlic extract. There was a distinctive increase in inhibition effects were observed by used of a combination with lactic acid bacteria and garlic extract.

Fatty Acid Profile of Synbiotic Cheese and its Effect on Intestinal Inflammation in Rats

2019 ◽  
Vol 14 (1) ◽  
pp. 45-50
Author(s):  
Nurliyani ◽  
Harmayani Eni ◽  
Rahmatulloh Satyaguna ◽  
Rakasivi Kanita Galih Julia
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Fatty Acid ◽  
Lactic Acid Bacteria ◽  
Fatty Acid Profile ◽  
Intestinal Inflammation ◽  
Lactobacillus Rhamnosus ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Chain Fatty Acids

Porang (Amorphophallus oncophyllus) is a local perennial plant rich in glucomannan. The objective of this study was to determine the effect of porang glucomannan addition during cheese processing on fatty acid profile, organic acid and vitamin B6 of goat milk cheese ripened with Lactobacillus rhamnosus. In addition, the effect of cheese consumption on short-chain fatty acid profile in the caecum digesta of inflammatory rats was evaluated. We found that the addition of glucomannan to the cheese during its ripening increased the levels of myristic, pentadecanoic acid, and cis-oleic acids. Rats consuming this cheese had elevated cecal levels of propionic, butyric, total short-chain fatty acids, and lactic acid bacteria. Consumption of synbiotic cheese also decreased the intestinal inflammation via increasing the total lactic acid bacteria, propionic, butyric, and total short-chain fatty acids.

scholarly journals Pengaruh Penambahan Skim terhadap Karakteristik Dadih Susu Sapi yang Dibuat dalam Ruas Bambu Wuluh (Schizostachyum silicatum) di Bali

2020 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Mariya Ulfa ◽  
I Made Sugitha ◽  
Luh Putu Trisna Darmayanti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Water Content ◽  
Skim Milk ◽  
Fermented Milk ◽  
Cow Milk ◽  
Total Acid ◽  
Bacterial Colony ◽  
Randomized Design ◽  
The Right

This study aims to determine the effect of skim additional on the characteristics and the amount of the right skim additional of cow milk dadih made in wuluh bamboo (Schizostachyum bluneiness) tubes. The completely randomized design with 5 levels of skim addition treatment were : 4%, 6%, 8%, 10% and 12%. The treatment was repeated 3 times for obtain 15 experimental units. The data were analyzed by variance, and the significant effect of the treatment was followed by Duncan. The additional of skim has effect to yield, water content, pH, total acid, total microbes, total lactic acid bacteria (LAB) and has effect to scoring test of aroma, taste and overall acceptance by hedonic test, but doesn’t significantly affect scoring test of color and texture. The addition of 10% skim produces the best characteritics with yield 65,31%, water content 63,72%, pH 5,50, total lactic acid 1,31%, total bacterial colony 4,38 x 109CFU/g, total lactic acid bacteria (LAB) 3,42 x 109 CFU/g, color cream, typical aroma of fermented milk, solid texture, sour taste and rather dislike on overall acceptance. Keywords : cow milk dadih, skim milk, wuluh bamboo.

Pengaruh Penambahan Starter Yogurt Dan Susu Skim Terhadap Kualitas Yogurt Susu Kacang Hijau (Vigna Radiata L)

2019 ◽  
Vol 6 (01) ◽  
pp. 1-16
Author(s):  
Aryati Rambu Nalu ◽  
Kukuk Yudiono ◽  
Sri Susilowati
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Protein Content ◽  
Raw Materials ◽  
Skim Milk ◽  
Amino Acid Content ◽  
Green Bean ◽  
Total Acid ◽  
Taste Test ◽  
Animal Milk

Yogurt is a type of beverage classified as a refreshment drink which is produced through a fermentation process that uses Lactic Acid Bacteria. Most legumes have been widely used as raw materials in the making of a milk known as vegetable milk. Legume milk contains amino acids that are almost equivalent to the amino acid  content of animal milk so it can be used as a base substitute material for fermented animal milk. Processed green bean milk yogurt is a form of food diversification. Skim milk is a low- fat milk added in the production of green bean milk yogurt, and yogurt starter is an important factor in the process of yogurt making. The purpose of the study is to determine the effects of the addition of yogurt starter and skim milk on the quality of green bean milk. The research design is 2-Factor Factorial Design arranged with Completely Randomized Design (CRD). Factor I consists of 2 levels, namely 12 grams of skim milk and 15 grams of skim milk; factor II consists of 3 levels, namely 15 ml, 20 ml, and 25 ml of starters. Observation variables consist of pH, total acid, viscosity, protein content, lactic acid bacteria, organoleptic aroma test, organoleptic texture test, and organoleptic taste test. From the results of this study, the highest results obtained in the treatment consist of: a) 12 grams of skim milk and 25 ml of 8.13%,Total Acid, Organoleptic Aroma Test 4.57, Organoleptic Texture Test 4.73; b) 15 grams of skim milk and 25 ml of starter of pH 4.17, Viscosity 8.67 cP, protein content 0.94%, Lactic Acid Bacteria 2933.33 x 103 CFU / mL, Organoleptic Aroma Test 4.57, Organoleptic Texture Test 4.61, Organoleptic Taste Test 5.02.

scholarly journals Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

2014 ◽  
Vol 66 (3) ◽  
pp. 940-948 ◽  
Author(s):  
L.B. Acurcio ◽  
M.R. Souza ◽  
A.C. Nunes ◽  
D.L.S. Oliveira ◽  
S.H.C. Sandes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gastric Ph ◽  
Probiotic Potential ◽  
Sheep Milk ◽  
Potential Evaluation ◽  
And Staphylococcus Aureus

Lactic acid bacteria species were molecularly identified in milk from Lacaune, Santa Inês and crossbred sheep breeds and their in vitro probiotic potential was evaluated. The species identified were Enterococcus faecium (56.25%), E. durans (31.25%) and E. casseliflavus (12.5%). No other lactic acid bacteria species, such as lactobacilli, was identified. Most of the isolated enterococci were resistant to gastric pH (2.0) and to 0.3% oxgall. All tested enterococci were resistant to ceftazidime, oxacillin and streptomycin and sensible to clindamycin, erythromycin and penicillin. The resistance to ciprofloxacin, gentamicin, tetracycline and vancomycin varied among tested species. All tested enterococci strongly inhibited (P<0.05) Escherichia coli and Listeria monocytogenes, moderately inhibited E. faecalis and Staphylococcus aureus and did not inhibit Pseudomonas aeruginosa, Salmonella enterica var. Typhimurium and also one E. durans sample isolated from sheep milk. Four samples of E. faecium, one of E. durans and one of E. casseliflavus presented the best probiotic potential.

scholarly journals POTENSI KOMBUCHA DAUN TEH (Camellia sinensis) DAN DAUN KOPI ROBUSTA (Coffea robusta) SEBAGAI MINUMAN PROBIOTIK

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Elok Zubaidah ◽  
Kiki Fibrianto ◽  
Soviandini Dwiki Kartikaputri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Lactic Acid ◽  
Bile Salts ◽  
Tea Leaves ◽  
Total Acid ◽  
Robusta Coffee ◽  
Multiple Attribute ◽  
Kombucha Tea

Kombucha is a plant-based fermented beverage that contains probiotic bacteria such as lactic acid bacteria (LAB). This research was conducted to prove the potential of probiotics in LAB isolates of kombucha tea leaves and robusta coffee leaves with various concentrations. This research used a completely randomized design (CRD). The variables tested were the measurement of total lactic acid bacteria, total acid, and probiotic characterization of LAB isolates (LAB resistance to low pH and bile salts, and antibacterial activity test). The results were analyzed and discussed using ANOVA with a significance level of P < 0.05 only for total acid and descriptive analysis on microbiological response. The best probiotic potency was selected using the multiple attribute method. The results showed that LAB isolates from kombucha tea leaves and robusta coffee leaves with various concentrations had resistance to pH 2 and pH 3, resistance to bile salts 3%, and antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. The LAB isolate of kombucha robusta coffee leaves at a concentration of 0.6% has the best probiotic potential. Kombucha merupakan minuman fermentasi berbasis tanaman yang mengandung bakteri probiotik seperti bakteri asam laktat (BAL). Penelitian ini dilakukan untuk membuktikan potensi probiotik pada isolat BAL kombucha daun teh dan daun kopi robusta dengan berbagai konsentrasi. Penelitian laboratorium menggunakan rancangan acak lengkap (RAL). Variabel yang diuji adalah pengukuran total bakteri asam laktat, total asam, ketahanan BAL terhadap pH rendah dan garam empedu, serta uji aktivitas antibakteri. Hasil penelitian dianalisa dan dibahas menggunakan ANOVA dengan signifikansi P < 0.05 hanya untuk total asam dan analisa deskriptif pada respons mikrobiologis. Potensi probiotik terbaik dipilih menggunakan metode multiple attribute. Hasil penelitian menunjukkan bahwa isolat BAL dari kombucha daun teh dan daun kopi robusta dengan berbagai konsentrasi memiliki ketahanan terhadap pH 2 dan pH 3, ketahanan terhadap garam empedu 3%, serta aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolat BAL kombucha daun kopi robusta pada konsentrasi 0,6% mempunyai potensi probiotik terbaik.

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