Isolasi Bakteri Endofit Dari Sea Grass Yang Tumbuh Di Kawasan Pantai  Pulau Lombok Dan Potensinya Sebagai Sumber Antimokroba  Terhadap Bakteri Patogen

ABSTRAK Telah dilakukan isolasi bakteri endofit dari seagrass jenis Enhalus acoroides (Linneaus f.) Royle, yang tumbuh di perairan pantai Kuta Lombok Tengah. Media yang digunakan adalah NA, BHI, TSA dan Mac Conkey’ Agar, dengan menggunakan bagian akar batang, dan daun sebagai sumber isolat. Dilakukan subkultur berulang hingga diperoleh isolat endofit yang membentuk koloni tunggal.Terhadap koloni tunggal ini dilakukan identifikasi morfologi dan uji biokimia. Selanjutnya dilakukan uji aktivitas antibakteri dengan metode Difusi Agar (Disk diffusion test) dengan teknik sumuran menggunakan lima bakteri uji klinis yaitu : Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Shygella dysentriae, dan Staphylococcus. aureus. Kategori sensitivitas bakteri uji klinis terhadap ekstrak bakteri endofit mengacu kepada Mukherjee (1989). Identifikasi morfologi, uji biokimia dan uji aktifitas antibakteri dilakukan terhadap 11 isolat dari 28 isolat awal yang diperoleh diperoleh, Hasil menunjukkan bahwa semua isolat bakteri endofit tergolong dalam bentuk batang, 8 diantaranya termasuk Gram Positif. Hasil uji antibakteri menggunakan metode Difusi agar dengan teknik sumuran menunjukkan bahwa hampir semua isolat memiliki aktivitas antibakteri terhadap bakteri uji dengan daya hambat yang bervariasi. Diperoleh satu isolat yang palingkuat efeknya dan paling luas spektrumnya yaitu isolate 1 yang mampu menghambat pertumbuhan 4 jenis bakteri uji (E. coli, P. aeruginosa, K. pneumonia, S. dysentriae, dan S. aureus) hingga sampai pada kategori sensitif (dimeter zona hambat lebih dari 12 mm). Dapat disimpulkan bahwa isolat bakteri endofit yang berasal dari seagrass sukup potensial untuk dikembangkan sebagai salah satu alternatif sumber antibakteri untuk mengatasi penyakit yang disebabkan oleh bakteri pathogen. Kata Kunci: Antimikroba, bakteri endofit, lamun (sea grass), aktivitas antibakteri.ABSTRACTHave done the isolation of endophytic bacteria on seagrass kind Enhalus acoroides (Linnaeus f.) Royle, who grew up in the coastal waters of Kuta Lombok. The medium used is NA, BHI, TSA and Mac Conkey 'Agar, using the roots stems and leaves as a source of isolates. Subcultures performed repeatedly to obtain isolates endophytic form colonies tunggal.Terhadap single colony is to identify morphological and biochemical tests. Furthermore, the antibacterial activity test by the method Diffusion Agar (Disk diffusion test) with wells technique uses five clinical trials that bacteria: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Shygella dysentriae, and Staphylococcus. aureus. Categories sensitivity of bacteria clinical trials of endophytic bacteria extract refers to Mukherjee (1989). Identification of morphological, biochemical tests and antibacterial activity test conducted on 11 isolates of 28 isolates obtained initial obtained results showed that all isolates of endophytic bacteria belong in the form of rods, 8 of which include Gram Positive. Antibacterial test results using the diffusion method in order to pitting technique showed that almost all isolates possess antibacterial activity against bacteria inhibition test with varied. Retrieved palingkuat one isolate its effects and the most extensive spectrum that isolate one capable of inhibiting the growth of four kinds of test bacteria (E. coli, P. aeruginosa, K. pneumoniae, S. dysentriae, and S. aureus) to arrive at a sensitive category (dimeter inhibitory zone more than 12 mm). It can be concluded that endophytic bacteria isolates originating from sukup seagrass potential to be developed as an alternative source of antibacterial to treat diseases caused by pathogenic bacteria.Keywords: Antimicrobial, endophytic bacteria, seagrass (sea grass), antibacterial activity.

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SENSITIVITAS ANTIMIKROBA TERHADAP BAKTERI ESCHERICHIA COLI YANG DIISOLASI DARI UDANG DI PASAR KEPUTRAN SURABAYA

JURNAL KAJIAN VETERINER ◽

10.35508/jkv.v7i2.1978 ◽

2020 ◽

Vol 7 (2) ◽

pp. 93-100

Author(s):

Reina Puspita Rahmaniar ◽

Dyah Widhowati ◽

Nurul Hidayah

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antibiotic Sensitivity ◽

Mechanisms Of Resistance ◽

Diffusion Test ◽

Biochemical Tests ◽

Disk Diffusion Test ◽

E Coli ◽

Gram Staining ◽

Antibiotic Sensitivity Test

The aimed of this study was to identify antibiotic Resistance of Escherichia coli isolated from Shrimp. There were 20 samples taken and isolated on surface of Eosin Methilen Blue Agar. E.coli produced metalic green colonies. Samples identified as E.coli based on macroscopic features and morphology of colonies, microscopic examination with gram staining and biochemical tests. The antibiotic sensitivity was determined through a standard antimicrobial disk diffusion test. The results of this study showed that 9 samples were bacteria of the spesies E. coli. Based on the results of antibiotic sensitivity test, it was found that 100 % of E. coli isolates were resistant to Amoxycillin 20 µg and 22,2 % of isolates are resistant to ampicillin 10 µg. Antibiotics Tetracycline 30 µg and Chloramphenicol 30 µg were 100 % sensitive. Gentamicin 10 µg showed that 77,8 % sensitive and 22,2 % intermediet. The precence of antibiotic resistance of betalaktam antibiotics makes it especially important to monitor antimicrobial susceptibility and mechanisms of resistance of E.coli isolated from shrimp, because new mechanisms of resistance occurring in animals may enter the food chain and its be transferred to the human. This importance of cooperation between sectors in order to monitor antimicrobial resistance.

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Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽

10.35799/jm.3.2.2014.5989 ◽

2014 ◽

Vol 3 (2) ◽

pp. 129

Author(s):

Megawati S. Saroinsong ◽

Febby E. F. Kandou ◽

Adelfia Papu ◽

Marina F. O. Singkoh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methanol Extract ◽

Inhibition Zone ◽

E Coli ◽

Activity Test ◽

And Staphylococcus Aureus ◽

Paper Disc ◽

Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽

10.22487/bioceb.v15i2.15540 ◽

2022 ◽

Vol 15 (2) ◽

pp. 90-97

Author(s):

Gaby Maulida Nurdin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Lantana Camara ◽

Diffusion Method ◽

E Coli ◽

Activity Test ◽

Growth Inhibitory ◽

One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

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ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI ENDOFIT KULIT BATANG SRIKAYA (ANNONA SQUAMOSA) DAN POTENSINYA SEBAGAI ANTIBAKTERI

Jurnal Penelitian Pendidikan IPA ◽

10.29303/jppipa.v4i1.98 ◽

2018 ◽

Vol 4 (1) ◽

Author(s):

Lalu Zulkifli ◽

Dwi Soelistya Dyah Jekti ◽

Samsul Bahri

Keyword(s):

Antibacterial Activity ◽

Endophytic Bacteria ◽

Pathogenic Bacteria ◽

Positive Control ◽

Spore Formation ◽

Bacterial Isolates ◽

Annona Squamosa ◽

Biochemical Tests ◽

Bacillus Brevis ◽

E Coli

The objective of this study was to isolate endophytic bacteria from bark of srikaya, analyzing antibacterial activity of endophytic bacteria in S. aureus, B. cereus and E. coli pathogenic bacteria, characterizing endophytic bacteria capable of inhibiting the growth of pathogenic bacteria and identification of endophytic bacteria able to inhibit the growth of pathogenic bacteria. Isolation of endophytic bacteria using TSA and NA media, bioassay on pathogen bacteria with concentration of 106 cells / ml with using Ø 6 mm wells and entering supernatant of 100 μl. Supernatant was obtained by growing endophytic bacteria in NB media shaken with shaker 150 cycles / min for 48 h at 32ᵒC then culture centrifuge at 5000 g for 30 min. Positive control using cyprofloxacin. Characterization is based on the nature of the colony, Gram paint, spore formation, and biochemical tests. The results of the study yielded 13 endophytic bacterial isolates and 4 endophytic isolates capable of inhibiting the growth of 8 pathogenic bacteria with sensitive criteria, 2 pathogenic bacteria with resistant criteria and 1 pathogen bacteria can not be inhibited its growth. Gram's paint results show that 4 endophytic isolates belong to Gram-positive, rod-shaped and spore forming cells. From the character possessed by the bacteria can be identified that the 4 bacteria endofit capable of inhibiting pathogenic bacteria are Bacillus brevis, Bacillus latesporus, Virgibacillus pantothenticus, and Bacillus circulansKeywords: Characterization, Endophytic Bacteria, Bark of Srikaya, Antibacterial

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Molecular detection of multidrug and colistin-resistant Escherichia coli isolated from house flies in various environmental settings

Future Microbiology ◽

10.2217/fmb-2019-0053 ◽

2019 ◽

Vol 14 (10) ◽

pp. 847-858 ◽

Cited By ~ 7

Author(s):

Abdus Sobur ◽

Zobayda F Haque ◽

Abdullah AM Sabuj ◽

Samina Ievy ◽

AMM Taufiqur Rahman ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistant ◽

Diffusion Test ◽

Isolation And Identification ◽

Antibiotic Resistant ◽

Disk Diffusion Test ◽

House Flies ◽

E Coli ◽

Pathogenic Escherichia Coli ◽

Antimicrobial Resistance Genes

Aim: To detect antibiotic-resistant pathogenic Escherichia coli in house flies captured from various environmental settings. Materials & methods: Isolation and identification of E. coli was performed based on culture and PCR. Antimicrobial susceptibility testing was conducted using disk diffusion test. Detection of virulence and antimicrobial resistance genes was carried out using PCR methods. Results: The prevalence of E. coli in flies was 61% with highest in dairy farms (70.67%) followed by food centers (65.33%), dustbins (64%) and area close to hospital (44%). The prevalence rates of tetA, tetB, ereA, CITM, SHV, mcr-3 and catA1 resistance determinants were 88.75, 48.52, 41.67, 37.17, 27.77, 18.37 and 14.81%, respectively. Conclusion: Multidrug-resistant pathogenic E. coli are widely distributed in house flies, which requires a one-health approach to delineate their control.

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Antibacterial Activities of Rough Lemon (Citrus jambhiri Lush) Rind Essential Oil

Journal of Chemical Natural Resources ◽

10.32734/jcnar.v1i1.830 ◽

2019 ◽

Vol 1 (1) ◽

pp. 12-18

Author(s):

Helmina Sembiring

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Chemical Compounds ◽

Antibacterial Activities ◽

Diffusion Test ◽

Citrus Jambhiri ◽

Disk Diffusion Test ◽

Rough Lemon ◽

Activity Test ◽

Paper Disk

The isolation of rough lemon (Citrus jambhiri Lush) rind essential oil has been done by using hydrodistillation method and paper disk diffusion test for antibacterial activity test. 2.3 gram (1.51%) of essential oil was obtained from 450 gram of dried rough lemon rind powder. Based on the analysis using GC-MS, the essential oil from rough lemon rind contains 5 chemical compounds, but only 4 of them can be interpreted. They are Limonene (71.88%), 1,4-cyclohexadiene (13.93%), β-Pinene (10.57%) and β-Ocimene (2.37%). Antibacterial activity was tested to Bacillus cereus and Escherichia coli at 40% concentration (v/v) with 12.7 mm and 12.9 mm of clear zones respectively.

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Aktivitas Antibakteri TiO2-Anatas Terdadah -Vanadium dan -Kromium

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.12.2.1744.112-127 ◽

2016 ◽

Vol 12 (2) ◽

pp. 112

Author(s):

Hari Sutrisno ◽

Ariswan Ariswan ◽

Dyah Purwaningsih

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Dilution Method ◽

Test Results ◽

E Coli ◽

Activity Test ◽

Growth Of Bacteria

Samples of vanadium- and -chromium doped TiO2-anatas have been conducted antibacterial activity against the bacteria Escherichia coli (E. Coli). The minimum kill concentration (MBC) against the bacteria of E. Coli is determined by liquid dilution method. The antibacterial activity test of 0; 2.3; 3.3; 4.9% wt. vanadium doped TiO2-anatas and 0; 1.1; 3.9; 4.4% wt. chromium doped TiO2-anatas have been performed against bacteria of E. Coli in the absence of light (dark). The test results indicate that the presence of 3.3 and 4.9 in %wt. vanadium-TiO2-anatas are able to inhibit the growth of bacteria E. Coli, contrary all chromium doped TiO2-anatas are not able to inhibit the growth of bacteria of E. Coli.

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Antibacterial activity of granulated carbon sorbents

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2017.03.76-82 ◽

2017 ◽

pp. 76-82

Author(s):

В.Т. Долгих ◽

Л.Г. Пьянова ◽

Т.И. Долгих ◽

В.А. Лихолобов ◽

О.В. Корпачева ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Effect ◽

Antibacterial Properties ◽

Petri Dish ◽

Klebsiella Pneumonia ◽

Carbon Sorbents ◽

E Coli ◽

Petri Dishes

Цель исследования - изучение антибактериальной активности гранулированных углеродных сорбентов по отношению к патогенной микрофлоре в условиях различной продолжительности контакта с сорбентом. Методика. Исследовали антибактериальные свойства гранулированного сорбента ВНИИТУ-1, обработанного гидромеханически, окисленного и стабилизированного до нормативных значений рН и модифицированного поли-N-винилпирролидоном (ПВП) ВНИИТУ-1 по отношению к патогенным микроорганизмам: Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia, Escherichia coli, Streptococcus agalactiae, а также к их смесям. Все микроорганизмы представляли собой клинические штаммы, выделенные из ран пациентов. Предварительно тест-микроорганизмы были исследованы на чувствительность к современным антибиотикам. Опытным путем подбирали такие разведения и количество засеваемого материала, чтобы выросшие на чашке Петри колонии можно было сосчитать. Бактерии сорбировали из физиологического раствора с концентрацией микробных клеток 3 х 10 колониеобразующих единиц в 1 мл исследуемой пробы (КОЕ/мл). В пробирку типа Эппендорф вносили сорбент в количестве 0,5 мл, добавляли 1 мл микробной взвеси, встряхивали для удаления пузырьков воздуха и выдерживали в термостате в течение 1, 3, 6 и 24 ч. По истечении заданного времени контакта сорбента с патогенной микрофлорой отбирали надосадочную жидкость в объеме 100 мкл, засевали на стерильные агаровые пластины чашек Петри с питательной средой ГМФ-агар. Засеянные чашки Петри помещали в СО-инкубатор 15АС (Япония) вверх дном и инкубировали при температуре 37 ± 1°С в течение 24 ± 2 ч. При подсчете колоний, выросших на чашке Петри, учитывали только те чашки, на которых выросло более 300 изолированных колоний. Результаты. Гранулированный сорбент ВНИИТУ-1 обладает антибактериальным действием в отношении как грамположительных, так и грамотрицательных бактерий, а также их смеси. Сроки проявления антибактериального эффекта сорбента зависят от вида микроорганизмов и времени их контакта с образцом. Присутствие в составе сорбента модификатора ПВП усиливает его антибактериальные свойства. Полимермодифицированный образец обладает пролонгированным антибактериальным эффектом в отношении широкого спектра микроорганизмов (E. coli, St. aureus, P. aeruginosae и St. aureus) и смеси их культур. Заключение. Показана возможность повышения антибактериальных свойств гранулированных углеродных сорбентов по отношению к патогенной микрофлоре путем их модификации поли-N-винилпирролидоном. Дальнейшая разработка и применение углеродных сорбентов как средств сорбционной терапии является перспективным подходом к местному лечению гнойных ран. The purpose: Study the antibacterial activity of granulated carbon sorbents in relation to the pathogenic microflora under the conditions of different period of contact with sorbent. Methods. It has been investigated the antibacterial properties of VNIITU-1 granulated sorbent, which was hydromechanically treated, oxidized and stabilized up to pH standard parameters and VNIITU-1 modified poly-N-vinylpyrrolidone (PVP) against the pathogenic micro-organisms, including Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia, Escherichia coli, Streptococcus agalactiae, and their mixes. All bacteria were clinical strains derived from the wounds of patients. Dilutions and amount of inoculated material were experimentally selected for further calculation of the grown colonies on the Petri dish. Bacteria were sorbed from the saline solution with bacteria concentration of 3 х 10 of the colony forming units in 1 ml of test sample (CFU/ ml). Eppendorf tube was added the sorbent 0.5 ml and bacterial suspension 1 ml; then the tube was shaken to remove the air bubbles and incubated for 1, 3, 6 and 24 hr. Upon the expiry of the target time for sorbent with pathogenic flora contact the supernatant fluid was collected in 100 ul and inoculated on the sterile agar plates of the Petri dishes with GMF agar nutrient medium. Inoculated Petri dishes were placed upside down in СО incubator 15AC (Japan) and incubated at 37 ± 1°C for 24 ± 2 hours. When counting the colonies growing on the Petri dish, they took into consideration only the dishes where more than 300 isolated colonies were grown. If more than 300 colonies were grown, «> 3x102 CFU/ml» was recorded in the protocol. Results. Granulated sorbent VNIITU-1 has an antibacterial effect against both gram-positive and gram-negative bacteria, and their mixes. Time period for manifestations of the sorbent antibacterial effect depends on the type of microorganisms and time of contact. PVP presence in sorbent composition enhances the antibacterial effect of the sorbent. Resin-modified sample has a prolonged antibacterial effect against a broad spectrum of microorganisms (E. coli, St. aureus, P. aeruginosae and St. aureus) and mixtures of their cultures. Conclusion. It has been determined the possibility for increasing of antibacterial properties of granulated carbon sorbents against the pathogenic microflora by their modification with poly-N-vinylpyrrolidone. Further development and application of carbon sorbents as the means for sorption therapy is a perspective approach for the local treatment of purulent wounds.

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Molecular characterization of enterohemorrhagic Escherichia coli isolated from diarrhea samples from human, livestock, and ground beef in North Jordan

Veterinary World ◽

10.14202/vetworld.2021.2827-2832 ◽

2021 ◽

pp. 2827-2832

Author(s):

Yaser H. Tarazi ◽

Saeb N. El-Sukhon ◽

Zuhair Bani Ismail ◽

Amani A. Almestarehieh

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogen ◽

Ground Beef ◽

Enterohemorrhagic Escherichia Coli ◽

Diffusion Test ◽

Disk Diffusion Test ◽

Fecal Samples ◽

E Coli ◽

Beef Meat ◽

Meat Samples

Background and Aim: Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen with worldwide distribution. Data regarding its presence, distribution, virulence, and antimicrobial susceptibility among various animal species and humans in Jordan are lacking. Therefore, the objectives of this study were to isolate and characterize EHEC from human and animal diarrhea fecal samples and ground beef samples. Materials and Methods: A total of 100 and 270 diarrhea fecal samples from humans and animals, respectively, were collected. In addition, 40 ground beef meat samples were collected from retail markets. EHEC was positively identified by detecting Shiga toxins (stx1 and stx2) genes using multiplex polymerase chain reaction (PCR). Antimicrobial susceptibility patterns were determined using the disk diffusion test. Beta-lactamase production was detected using the double disk diffusion test and the extended-spectrum beta-lactamases (ESBLs) were identified by detection of blaTEM, blaSHV, and OXA-1 genes using multiplex PCR. Pulsed-field gel electrophoresis (PFGE) was used to investigate the relatedness of EHEC isolates from different sources. Results: Out of 410 samples, 194 E. coli isolates were positively identified, of which 57 isolates (29%) were classified as EHEC. Thirty-five (61%) of EHEC isolates were serotyped as O157 (19: O157:H7 and 16: O157:NM). The stx1 gene was detected only among the sheep and goats isolates at a rate of 7.6% and 5.2%, respectively, while the stx2 gene was detected in only one ground beef meat sample. EHEC isolates showed high resistance patterns against amoxicillin, gentamycin, cephalexin, and doxycycline. Twenty-four out of 32 EHEC isolates were determined as ESBL producers, among which 14 isolates expressed the blaSHV gene and 19 isolates expressed the blaTEM while four expressed both genes. PFGE analysis revealed two clusters with high similarity (92%) originated from ground beef meat and cattle fecal samples. No similarities were found between human and animal E. coli isolates. Conclusion: Results of this study indicate widespread ESBL EHEC among humans, animals, and ground beef meat samples. These results represent an important alarm that requires the implementation of appropriate preventative measures by both human and animal health sectors to prevent the transmission of this important foodborne pathogen.

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SINTESIS DAN KARAKTERISASI NANOPARTIKEL CuFe2O4 SERTA APLIKASINYA SEBAGAI ANTIBAKTERI

CHEMISTRY PROGRESS ◽

10.35799/cp.12.2.2019.27929 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Almendo G. Lasera ◽

Henry Aritonang ◽

Harry Koleangan

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Gram Negative Bacteria ◽

E Coli ◽

Activity Test ◽

Sem Imaging ◽

Scanning Electron

ABSTRAKNanopartikel CuFe2O4 disintesis menggunakan metode kopresipitasi. Material yang telah disintesis, kemudian dikarakterisasi dengan menggunakan Scanning Electron Microscopy (SEM) dan Xray-Diffraction (XRD) serta di uji aktifitasnya sebagai antibakteri dengan menggunakan bakteri Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil citra SEM memperlihatkan morfologi nanopartikel CuFe2O4 berbentuk persegi yang tidak beraturan. Difraktogram XRD menunjukkan bahwa ukuran sampel CuFe2O4 yang diperoleh adalah sebesar 20,136 nm. Uji aktifitas antibakteri yang telah dilakukan menunjukkan bahwa, nanopartikel CuFe2O4 jauh lebih baik dalam menghambat pertumbuhan bakteri E. coli dibandingkan dengan bakteri S.aureus, masing-masing 24 mm dan 6,5 mm. ABSTRACTCuFe2O4 nanoparticles were synthesized using the coprecipitation method. The synthesized material was then characterized using Scanning Electron Microscopy (SEM) and Xray-Difraction (XRD) and tested as antibacterial activity using Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) bacteria. SEM imaging showed morphology of CuFe2O4 nanoparticles shaped like a rod. XRD diffractogram showed that the size of CuFe2O4 nanoparticles that obtained at 20.13 nm. The antibacterial activity test that has been done shows, CuFe2O4 nanoparticles are much better in inhibiting the growth of E. coli bacteria compared with S. aureus bacteria, 24 mm and 6,5 respertivelly.

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