scholarly journals Multidrug resistant Staphylococcus aureus; its incidence and antibiotic sensitivity pattern.

2021 ◽  
Vol 28 (11) ◽  
pp. 1590-1594
Author(s):  
Saeeda Nabat ul Hassan ◽  
Khushbu Farva ◽  
Ghulam Asghar Bhutta
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Conventional Technique ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Characterization Methods ◽  
Sensitivity Pattern

Objective: To determine incidence of MRSA in our community and its antibiotic susceptibility pattern. Study Design: Cross Sectional study. Setting: Department of Pathology, Sahara Medical College Narowal. Period: January 2020 to June 2020. Material & Methods: Bacterial isolates were taken from the samples of blood, pus and other body fluids sent to the hospital laboratory to determine culture and sensitivity pattern. Those samples positive for staphylococcus aureus were included in the study for further examination. MRSA were detected using conventional technique (catalase, coagulase and DNase methods) and confirmed by phenotypic and molecular characterization techniques (cefoxitin and oxacillin sensitivity, mec-A gene and 16S rRNA genes sequencing methods). Their antibiotic sensitivity was determined using standard Kirby Bauer disc diffusion technique. Chi square test was applied on collected data using SPSS-24. Results: Total 158 bacterial isolates containing staphylococcus aureus were included in the study. MRSA (multi drug resistant staphylococcus aureus) was detected in 33.5% samples and MSSA (multi drug sensitive staphylococcus aureus) was detected in 66.5% samples. Frequency of MSSA was more than MRSA. Male gender was more affected (60%) than female (40%). All bacterial isolates containing staphylococcus aureus were resistant to oxacillin and cefoxitin. MRSA isolates were sensitive to vancomycin, linezolid, teicoplanin, fosfomycin and fusidic acid, rifampicin, clindamycin, minocyclin and chloramphenicol, while resistant to penicillin, azithromycin, ciprofloxacin, erythromycin and co-trimoxazole. Conclusion: MRSA is detected by conventional technique followed by phenotypic and molecular characterization methods for confirmation. Methicillin sensitive staphylococcus aureus was detected more commonly than methicillin resistant staphylococcus aureus in our study sample. Proper culture and antibiotic sensitivity pattern is necessary to deal with MRSA.

scholarly journals Profile and Antibiotic Susceptibility Pattern of Bacterial Isolates from Mobile Phones of Healthcare Workers in a Tertiary Care Centre of Nepal

2020 ◽  
Vol 42 (2) ◽  
pp. 29-32
Author(s):  
Jyotshna Sapkota ◽  
Beena Jha ◽  
Bhavesh Mishra ◽  
Deepti Shrestha ◽  
Achut Barakoti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mobile Phones ◽  
Healthcare Workers ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Bacterial Isolates ◽  
Tertiary Care Centre ◽  
Cross Sectional ◽  
Sensitivity Pattern

Introduction Nosocomial infection is a serious problem worldwide. Healthcare workers mobile phones may act as vehicle for nosocomial infections. Mobile phones are rarely cleaned and healthcare workers frequently use them thorough out their time in hospitals. The objective of this study is to determine the rate of contamination of mobile phones, prevalent microorganisms and their antibiotic sensitivity pattern. MethodsThe descriptive cross-sectional study was carried out in Clinical Microbiology laboratory from July 2019 to September 2019 after ethical approval. Samples were collected aseptically by rolling sterile swabs over the exposed surfaces of the mobile phones and inoculated on the agar plates and incubated aerobically. After incubation, plates were examined for growth. Bacteria were identified by standard microbiological procedure and antibiotic sensitivity test of isolated pathogens was done using Muller Hinton Agar by Kirby-Bauer method. Statistical analysis was done by Excel 2018. ResultsOf the 198 mobile phones sample cultures, 112 (56.6%) samples showed 7 different types of bacteria. Mixed growth was observed in 3 mobile phones and total of 115 bacterial isolates were identified. Staphylococcus species (74.7%) were predominant organism followed by Acinetobacter spp, Klebsiella pneumoniae, E. coli, Citrobacter and Pseudomonas aeruginosa. Total 37.5% of Staphylococcus aureus were methicillin-resistant Staphylococcus aureus (MRSA). ConclusionMobile phones may have potential pathogenic microorganisms on their surface and can act as vector in spread of infectious agents in hospital settings. It is recommended to disinfect mobile phones regularly and to implement hand washing practices and regulations around the use of mobile phones in hospital settings.

scholarly journals Frequencies, sensitivity pattern and molecular characterization of bacterial isolates in blood in neonatal sepsis.

2021 ◽  
Vol 28 (7) ◽  
pp. 987-992
Author(s):  
Saeeda Nabat ul Hassan ◽  
◽  
Ghulam Asghar Bhutta ◽  
Khushbu Farva ◽  
◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bacterial Growth ◽  
Neonatal Sepsis ◽  
Antibiotic Sensitivity ◽  
Sampling Technique ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Gram Negative ◽  
Antimicrobial Drug Resistance ◽  
Sensitivity Pattern

Objective: To determine frequency, molecular characterization and sensitivity of bacterial isolates against commonly used antibiotics in neonatal sepsis. Study Design: Cross Sectional study. Setting: Department of Pathology Sahara Medical College Narowal. Period: October 2019 to March 2020. Material & Methods: Neonates admitted in neonatal intensive care unit (NICU) of study institution having signs and symptoms of neonatal symptoms such as fever, irritability, seizures, anorexia and lethargy, were included in the study using consecutive sampling technique. Blood sample from all study patients taken and sent for culture to determine bacterial isolates and antibiotic sensitivity against commonly used antibiotics for neonatal sepsis. Bacterial isolates identification was done using standard bacteriological technique performed by modified Kirby & Bauer disc diffuse method as per Clinical and Laboratory standards institute (CLSI) guidelines. Results: Total 200 cases were studied having neonatal sepsis and admitted in NICU including 58% female and 42% male children. Bacterial growth occurred in 10.5% samples and in 89.5% samples no bacterial growth seen. There were 1% samples with gram positive and 9.5% samples with gram negative bacterial isolates. Klebsiella was the commonest organism isolated in 38.1% cases out of total positive isolates. There were 72.5% neonates having age 1-14 days and 27.5% neonates having age 15-28 days. Conclusion: Gram negative bacteria are common cause of neonatal sepsis, out of which Klebsiella is the commonest organism. Antimicrobial drug resistance in different infections is a serious emerging issue.

scholarly journals Bacteria Sensitivity Pattern of Caesarean Section Wound on Selected Antibiotics in Ulin General Hospital Banjarmasin

2020 ◽  
Vol 16 (2) ◽  
pp. 73
Author(s):  
Nurhafizah Rafiani ◽  
Renny Aditya ◽  
Noor Muthmainah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Caesarean Section ◽  
Staphylococcus Epidermidis ◽  
Antibiotic Sensitivity ◽  
Cross Sectional ◽  
Surgical Wounds ◽  
Sensitivity Pattern ◽  
Antibiotic Sensitivity Test ◽  
Bacterial Sensitivity

Abstract: A cesarean delivery increases the risk of wound infection it should be prevented by using antibiotics. This study was to determine the pattern of bacterial sensitivity in surgical wounds of cesarean section patients for selected antibiotics, i.e ceftriaxone, cefixime, sulbactam ampicillin, ciprofloxacin, clindamycin and gentamicin.This observational study was conducted at the Ulin Public Hospital in Banjarmasin from July to September 2019. Using a cross sectional approach Samples of bacteria were identidied from 36 that were pusposively sampled, i.e., Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. Antibiotic sensitivity test showed that Staphylococcus aureus was sensitive against gentamicin (100%),whereas Staphylococcus epidermidis and Escherichia coli. Were intermediately sensitive towards gentamicin (62.5%) and ceftriaxone (80%) Keywords: Antibiotic susceptibility, caesarean section, surgical wound

scholarly journals Antibiotic Sensitivity Pattern of Bacterial Isolates in Patients with Chronic Rhinosinusitis in Kaduna, Nigeria

2018 ◽  
Vol 23 (02) ◽  
pp. 152-156
Author(s):  
Emmanuel Musa ◽  
Aliyu Kodiya ◽  
Abdullahi Kirfi ◽  
Onyekwere Nwaorgu
Keyword(s):  
Chronic Rhinosinusitis ◽  
Anaerobic Bacteria ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Bacterial Isolates ◽  
Statistical Parameters ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Sensitivity Tests ◽  
Aerobic And Anaerobic

Introduction A common practice in the management of patients with chronic rhinosinusitis (CRS), the empirical use of antibiotics may contribute to treatment failure and to the development of antimicrobial resistance. Objective To determine the antibiotic sensitivity pattern of aerobic and anaerobic bacteria associated with CRS. Methods This was a prospective cross-sectional study in which endoscopically guided middle meatal swabs (IBM Spss, version 16.0, Chicago, IL, USA) were aseptically taken from patients diagnosed with CRS after obtaining informed consent and ethical clearance. The samples were sent to the laboratory for qualitative and semiquantitative analysis via gram stain, aerobic, anaerobic cultures and antibacterial sensitivity tests. The collected data was analyzed using SPSS for Windows, version 16 (SPSS Inc, Chicago, IL, USA). Simple statistical parameters and paired sample t-test were used, as appropriate. Results There were 74 (56.92%) bacterial growths, out of which 55 (74.32%) were aerobic and 19 (25.68%) were anaerobic isolates, from a total of 130 patients. About 13 (17.5%–18%) of these bacterial growths yielded a mixed growth of aerobic and anaerobic isolates. The most common bacterial isolates were 26 (35.14%) Staphylococcus aureus, Haemophilus influenzae 9 (12.16%), Streptococcus viridians 8 (10.81%), and Streptococcus pneumoniae 5 (6.76%). Augmentin, ciprofloxacin, and Peflacine were found to be most effective, followed by levofloxacin, Rocephin, erythromycin and Zinat in that order. Conclusion Augmentin, ciprofloxacin and Peflacine have a sensitivity of 100%, while most of the organisms show resistance to Ampiclox, amoxicillin, and Septrin.

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

scholarly journals Neonatal Septicemia: Isolation, Identification and Antibiotic Sensitivity Pattern of Bacteria in a Tertiary Hospital in Bangladesh

2017 ◽  
Vol 11 (2) ◽  
pp. 58-61
Author(s):  
Mahmuda Afrin ◽  
Md Abdullah Siddique ◽  
Abdullah Akhtar Ahmed ◽  
Md Nazrul Islam ◽  
Parimol Chandra Sarker ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Brain Heart Infusion ◽  
Antibiotic Sensitivity ◽  
Gram Positive ◽  
College Hospital ◽  
Cross Sectional ◽  
Neonatal Septicemia ◽  
Microbiological Methods ◽  
Sensitivity Pattern ◽  
Highly Sensitive

A cross sectional descriptive study was done to find out the causative organisms and their antibiotic sensitivities in the Department of Microbiology in collaboration with the Department of Paediatrics, Rajshahi Medical College Hospital (RMCH), Rajshahi during the period of July 2014 to June 2015. A total of 116 blood samples were taken aseptically from patients who were suspected of neonatal septicemia. Blood was then inoculated in Brain heart infusion broth. Bacterial isolation, identification and antimicrobial susceptibility testing were done by standard microbiological methods. Among 116 cases, 33(28.4%) were found to be culture positive. The most commonly isolated causative agents of neonatal septicemia were Staphylococcus aureus 17(51.5%) followed by Escherichia coli 10(30.3%), Streptococcus pneumoniae 03(9.09%), Klebsiella pneumoniae 02(6.06%) and Pseudomonas aeruginosa 01(3.03%). In general, all the Gram positive and Gram negative isolates were highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin. Gram positive bacteria were found to be particularly sensitive to vancomycin. They were moderately sensitive to ceftazidime followed in amoxicillin but were totally resistant to ampicillin. This study revealed that Staphylococcus aureus and E.coli are predominant causative organisms in neonatal septicemia and these are highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin.Faridpur Med. Coll. J. Jul 2016;11(2): 58-61

scholarly journals Multi-Drug Resistance of Bacterial Isolates among Dental Caries Patients

2016 ◽  
Vol 3 (1) ◽  
pp. 37-44 ◽  
Author(s):  
K Yadav ◽  
S Prakash ◽  
NP Yadav ◽  
RS Sah
Keyword(s):  
Dental Caries ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Natural Phenomenon ◽  
Multi Drug Resistance ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Background and Objectives: Dental caries is a well known major oral health problem in most developing countries which has multifactorial etiology caused by many facultatively anaerobes. S. mutans is the main pathogen associated with this disease. Recently Multi-Drug Resistant (MDR) species of S. mutans were identified from the dental caries patients against many commercial antibiotics. MDR is a natural phenomenon, posing a serious worldwide threat to public health. Several therapeutic agents are available to treat or prevent tooth decay, but still global burden of the disease with MDR are emerging. Therefore, the present study was designed for assessing the antibiotic sensitivity pattern of commercially available antibiotics.Material and Methods: This cross-sectional study was carried out by following Standard protocols of Bergey’s Manual of Systematic Bacteriology to isolate and identify the organism and further followed by antibiotic susceptibility test of bacterial isolates by disc diffusion method.Results: Streptococcus mutans (40%) was the most predominant to cause dental caries followed by S. aureus with 28.92. Gram positive isolates were found to be frequently resistant towards penicillin and tetracycline whereas Gram negative isolates were found to be Cotrimoxazole resistant.Conclusion: A high frequency of penicillin resistance in oral isolates and its co-resistance to erythromycin, tetracycline, gentamycin and amipicillin among the pateints was observed. The various awareness programmes should be facilitating the appropriate use of antibiotic to re-establish dominance over diseases must be implemented.Janaki Medical College Journal of Medical Sciences (2015) Vol. 3 (1):37-44

Bacteriology and antibiotic sensitivity pattern of ear discharge in patients with chronic otitis media; A cross sectional study conducted in tertiary care hospital of Peshawar.

2021 ◽  
Vol 28 (05) ◽  
pp. 671-676
Author(s):  
Mubassir Ullah ◽  
Muhammad Saleh Faisal ◽  
Ali Zaki ◽  
Azam Khan ◽  
Mamoor Khan ◽  
...  
Keyword(s):  
Otitis Media ◽  
Proteus Mirabilis ◽  
Chronic Otitis Media ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Bacterial Isolates ◽  
Sectional Study ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Klebsiella Spp

Objective: To determine the frequency of common bacteria and antibiotic sensitivity pattern of ear discharge in patients with chronic otitis media. Study Design: Descriptive Cross Sectional study. Setting: Department of ENT, Northwest General Hospital & Research Centre, Peshawar. Period: February to September 2019. Material & Methods: A total of 196 patients of both gender and age between 13 to 60 years with clinical diagnosis of active chronic otitis media were recruited through consecutive sampling technique. After detailed history and relevant examination, demographic data was recorded and pus specimens were collected from the infected ear on cotton swabs. They were sent to pathology laboratory of hospital for culture of common bacteria (E.coli, Pseudomonas aueroginosa, Proteus mirabilis, Staphylococcus aureus and Klebsiella spp) and their respective antibiotic sensitivity (Co-trimoxazole, Co-Amoxiclav, Ciprofloxacin, Ampicillin, Imipenem, Ceftriaxone and Cefixime). Results: Bacterial isolates were analyzed as 48% Staph aureus, 28% Pseudomonas, 12% Proteus mirabilis, 8% E. coli and 4% Klebsiella spp. Imipenem was sensitive to 94%, ceftriaxone 84%, ciprofloxacin 80%, co-trimoxazole 66%, ampicillin 63%, cefixime 45% and co-amoxiclav against 43% of total bacterial isolates. Conclusion: The common causative organism for active chronic otitis media was Staph aureus followed by Pseudomonas. Imipenem was the most sensitive antibiotic against majority of bacterial isolates followed by ceftriaxone and ciprofloxacin.

scholarly journals Prevalence of Methicillin Resistant Staphylococcus aureus and Antibiotic Susceptibility Pattern in a Tertiary Hospital in Nepal

2018 ◽  
Vol 16 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Laxmi Kant Khanal ◽  
Ram Prasad Adhikari ◽  
Ankita Guragain
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibiotic Sensitivity ◽  
College Teaching ◽  
Tertiary Hospital ◽  
Cross Sectional Study ◽  
Resistance Pattern ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Sensitivity Pattern

Background: Methicillin resistant Staphylococcus aureus is a global health challenge nowadays creating problem in antibiotic therapy. This study was aimed to generate resistance pattern of Methicillin resistant Staphylococcus aureus to various antibiotics in order to formulate antibiotic policy for control of Methicillin resistant Staphylococcus aureus in Nepal.Methods: This was a cross-sectional study conducted at the department of Clinical Microbiology, Nepal Medical College Teaching Hospital, from April 2015 to March 2016. A total of 142 S. aureus isolated from various clinical specimens. were screened for Methicillin resistant Staphylococcus aureus by cefoxitin disc method according to Clinical and Laboratory Standards Institute guidelines.Results: Out of 142 S. aureus isolates, 30 (21.1%) were detected as Methicillin resistant Staphylococcus aureus by cefoxitin disc method. Most of the Methicillin resistant Staphylococcus aureus (25/30) were isolated from pus which were collected from OPD patients. Antibiotic sensitivity pattern showed all Methicillin resistant Staphylococcus aureus isolates were sensitive to vancomycin.Conclusions: Prevalence of Methicillin resistant Staphylococcus aureus was found to be 21.1%, and all Methicillin resistant Staphylococcus aureus appear 100% sensitive to vancomycin. Keywords: .

scholarly journals Bacterial Assessment and Their Antibiotic Sensitivity Patterns of Nasal and Hand Swab Specimens From Food Handlers in Hospital Compounds in Mandalay, Myanmar: A Cross-sectional Descriptive or Observational Study

2020 ◽  
Author(s):  
Si Thu Aung ◽  
Aung Aung Nwe
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Food Handler ◽  
Food Handlers ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Nasal Swabs ◽  
The Government

Abstract Background: Microbial contamination of food can occur from contaminated or infected food handlers.Methods: This study aimed to assess type of bacteria and their antibiotic sensitivity pattern of nasal and hand swab samples from food handlers working in hospital compounds, Mandalay city, Myanmar. All eligible food handlers working in the government hospital compound, Mandalay City, Myanmar, were subjected to a cross-sectional study conducted from May to August 2018. Hand and nasal swabs were collected from of food handlers for bacterial isolation and identification. Antibiotic sensitivity patterns of isolated bacteria were determined.Results: Among 111 participants, 92 (82.9%) were bacteria-positive by hand swabs and 77(69.37%) were positive by nasal swab. Among Staphylococcus aureus isolated from nasal swabs, just over a half (51.2%) were resistant to oxacillin (probably methicillin resistant Staphylococcus aureus). There were association between hand contamination and nasal bacterial growth (p<0.001).Conclusion: Bacterial contamination status of the food handler working in hospital compound, Mandalay city, Myanmar, was extremely high.

Sign in / Sign up

Export Citation Format

Share Document