scholarly journals Effect of Vitamin C and Selenium on Some Physiological and Reproductive Characters in Adult Roosters Exposed to Oxidative Stress Induced by Hydrogen Peroxide

2012 ◽  
Vol 36 (0A) ◽  
pp. 32-42
Author(s):  
محمودسالم محمدشيت المعاضيدي
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Body Weight ◽  
Vitamin C ◽  
Sodium Selenite ◽  
Sperm Concentration ◽  
Experimental Period ◽  
The Body ◽  
Abnormal Sperm ◽  
Study Results

The present study was designed to investigate the effect of Vitamin C (450 mg/kg diet) and sodium selenite (0.5 mg/kg diet) in adult white Leghorn male chickens (30 weeks of age), whose concomitantly exposed to oxidative stress induced by hydrogen peroxide (0.5%) supplemented with drinking water for 6 weeks on reproductive performance. Semen were collected at 0, 2, 4 and 6weeks, The study results showed that hydrogen peroxide treatment caused a significant decrease in the body weight, sperm concentration, mass motility and individual motility during experimental period. Also a decrease in testosterone and testis glutathione concentration at the 6th week of the treatment, accompanied with a significant increase in dead and abnormal sperm percentage, testis malondialdehyde level compared with the control group. Histopathological changes revealed presence of necrosis and sloughing in the epithelial lining of the semineferous tubules and vacuolar degeneration of the supporting cells that fall in the lumen of the semineferous tubules and necrosis of interstitial cells. Vitamin C and sodium selenite with hydrogen peroxide caused a significant increase in body weight, sperm concentration, mass motility and individual motility during the experimental period, testosterone and glutathione level, accompanied with a significant decrease in dead and abnormal sperm percentages and Malondialdehyde level compared with hydrogen peroxide. In addition to the improvement in the histological picture of the semineferous tubules, mitosis germ cells were observed through their arrangement in circular tubules. It was concluded from this study that Vitamin C and Sodium selenite reverse the adverse effects produced by hydrogen peroxide on certain physiological and reproductive aspects in adult male chickens.

Growth performance, correlation and regression estimates of seven-chicken strains in South-Western Nigeria

2020 ◽  
Vol 45 (2) ◽  
pp. 40-48
Author(s):  
A. J. Atansuyi ◽  
U. C. Ihendu ◽  
C. A. Chineke
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Correlation Coefficients ◽  
Tail Length ◽  
Experimental Period ◽  
The Body ◽  
Coefficient Of Determination ◽  
Indigenous Chicken ◽  
Randomized Design ◽  
Study Results

This study was conducted to determine the growth performance, correlation and regression estimates of seven-chicken strains in South-western Nigeria using a total of 300 day-old chicks. The birds were divided into seven groups based on their strain. The seven strains are Normal feather (NF), Fulani ecotype (FE), Frizzle feather (FF), naked neck (NN) and Transylvania indigenous strains while Hubbard and Marshal were meat-type exotic chickens. There were forty- five (45) unsexed day-old chicks in each strain except the Frizzle feather that were 30 in number. Completely randomized design (CRD) was used for the trial that lasted for 8 weeks. The birds were fed experimental diets ad libitum throughout the period of the study. Results showed that there were significant differences (p<0.05) in the initial and final weights of the birds. It was observed that exotic strains weighed heavier (3569.73gHB) than their indigenous counterparts (1391.11gNF). However, the Fulani ecotype weighed heaviest (1840.99g) among Nigeria indigenous strains during the experimental period. This showed that FE strains are generally heavy breed chicken and could be incorporated into a meat producing indigenous chicken if improved upon. The result of the correlation coefficients showed that a very strong, positive and highly significant (P<0.001) relationship existed between body weights and linear body measurements as most of the values are (>0.40). All the body parameter examined had significant (p<0.01) and direct relationship with the body weight. Shoulder-to-tail length (STL) had the highest coefficient of 0.98.The high correlation estimates obtained in this study could be as a result of pleiotropy, heterozygosity or linkage of genes in the birds. The three functions were highly significant (p<0.05) for all the parameters studied. This shows that the functions well described the parameters. On the basis of coefficient of determination (R ), the body weight of poultry birds at any age can be predicted most accurately with BRG using cubic function.

scholarly journals Evaluation of Body Weight, Serum Lipid Profile, Glucose Homeostasis, Oxidative Stress and Hepatic Function in Healthy Mice Fed With Zinc Sulphate Supplementation

2021 ◽  
Vol 20 (1) ◽  
pp. 59-66
Author(s):  
Md Atiqur Rahman ◽  
Mohammad Tohidul Amin ◽  
Sayema Arefin ◽  
Dipty Rani Bhowmik ◽  
Md Abdur Rahman Ripon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Vitamin C ◽  
Glucose Homeostasis ◽  
Zinc Sulphate ◽  
Zinc Supplementation ◽  
Serum Triglyceride ◽  
Hepatic Function ◽  
The Body ◽  
Control Treatment

The present research study was designed to evaluate the effect of zinc supplementation on body weight, serum triglyceride, cholesterol, glucose homeostasis, oxidative stress, and hepatic function in mice. Mice were treated with zinc sulphate at an equivalent weight of 6.5 mg/kg-body weight elemental zinc for four weeks. Bodyweight, serum glucose, triglyceride, cholesterol, serum MDA, nitric oxide, vitamin C, and hepatic enzymes level were determined at the end of the study period. Data from this study showed that supplementation with zinc in mice maintained a balanced blood glucose homeostasis throughout the experimental period. Moreover, treatment with zinc showed a significant (p <0.05) decrease in serum triglyceride and cholesterol level along with a decrease in the body weight compared to control. Treatment with zinc significantly attenuated the rate of lipid peroxidation whereas increased the level of vitamin C and NO level. The protective effect of zinc on liver activity was observed. Treatment with zinc showed a strong negative association with serum total cholesterol (r= -0.934, p = 0.02), triglycerides level (r= -0.709, p = 0.05), and body weight (r= -0.899, p = 0.01). The present findings demonstrate that zinc supplementation can be helpful to maintain a glucose homeostasis, ameliorate hyperlipidemia, oxidative stress, and liver dysfunction. Therefore, zinc supplementation can be suggested to alleviate diseases associated with metabolic syndrome and oxidative stress. Dhaka Univ. J. Pharm. Sci. 20(1): 59-66, 2021 (June)

scholarly journals Rap1a Overlaps the AGE/RAGE Signaling Cascade to Alter Expression of α-SMA, p-NF-κB, and p-PKC-ζ in Cardiac Fibroblasts Isolated from Type 2 Diabetic Mice

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 557
Author(s):  
Stephanie D. Burr ◽  
James A. Stewart
Keyword(s):  
Oxidative Stress ◽  
Heart Failure ◽  
Hydrogen Peroxide ◽  
Cardiac Fibroblasts ◽  
The Body ◽  
Signaling Cascade ◽  
Diabetic Mice ◽  
Pkc Ζ ◽  
Type 2 Diabetic

Cardiovascular disease, specifically heart failure, is a common complication for individuals with type 2 diabetes mellitus. Heart failure can arise with stiffening of the left ventricle, which can be caused by “active” cardiac fibroblasts (i.e., myofibroblasts) remodeling the extracellular matrix (ECM). Differentiation of fibroblasts to myofibroblasts has been demonstrated to be an outcome of AGE/RAGE signaling. Hyperglycemia causes advanced glycated end products (AGEs) to accumulate within the body, and this process is greatly accelerated under chronic diabetic conditions. AGEs can bind and activate their receptor (RAGE) to trigger multiple downstream outcomes, such as altering ECM remodeling, inflammation, and oxidative stress. Previously, our lab has identified a small GTPase, Rap1a, that possibly overlaps the AGE/RAGE signaling cascade to affect the downstream outcomes. Rap1a acts as a molecular switch connecting extracellular signals to intracellular responses. Therefore, we hypothesized that Rap1a crosses the AGE/RAGE cascade to alter the expression of AGE/RAGE associated signaling proteins in cardiac fibroblasts in type 2 diabetic mice. To delineate this cascade, we used genetically different cardiac fibroblasts from non-diabetic, diabetic, non-diabetic RAGE knockout, diabetic RAGE knockout, and Rap1a knockout mice and treated them with pharmacological modifiers (exogenous AGEs, EPAC, Rap1a siRNA, and pseudosubstrate PKC-ζ). We examined changes in expression of proteins implicated as markers for myofibroblasts (α-SMA) and inflammation/oxidative stress (NF-κB and SOD-1). In addition, oxidative stress was also assessed by measuring hydrogen peroxide concentration. Our results indicated that Rap1a connects to the AGE/RAGE cascade to promote and maintain α-SMA expression in cardiac fibroblasts. Moreover, Rap1a, in conjunction with activation of the AGE/RAGE cascade, increased NF-κB expression as well as hydrogen peroxide concentration, indicating a possible oxidative stress response. Additionally, knocking down Rap1a expression resulted in an increase in SOD-1 expression suggesting that Rap1a can affect oxidative stress markers independently of the AGE/RAGE signaling cascade. These results demonstrated that Rap1a contributes to the myofibroblast population within the heart via AGE/RAGE signaling as well as promotes possible oxidative stress. This study offers a new potential therapeutic target that could possibly reduce the risk for developing diabetic cardiovascular complications attributed to AGE/RAGE signaling.

scholarly journals Milk yield and composition, feed intake and stocking rate of crossbread cows in tropical grasses managed in a rotational grazing system

2010 ◽  
Vol 39 (7) ◽  
pp. 1548-1557 ◽  
Author(s):  
Nelson Massaru Fukumoto ◽  
Julio Cesar Damasceno ◽  
Fermino Deresz ◽  
Carlos Eugênio Martins ◽  
Antônio Carlos Cóser ◽  
...  
Keyword(s):  
Body Weight ◽  
Milk Yield ◽  
Dry Matter ◽  
Experimental Period ◽  
The Body ◽  
Dry Matter Intake ◽  
Panicum Maximum ◽  
Stocking Rate ◽  
Rotational Grazing ◽  
Grazing System

The objective of this study was to evaluate milk yield and composition, dry matter intake, and stocking rate in pastures with tanzania grass (Panicum maximum cv. Tanzânia), star grass (Cynodon nlemfuensis cv. Estrela-Africana), and marandu grass (Brachiaria brizantha cv. Marandu). The grasses were managed in a rotational grazing system with Holstein x Zebu crossbreed cows, with a 30-day resting period and three days of paddock occupation. The pastures were fertilized with 1,000 kg/ha/year using the 20:05:20 (NPK) formula, split in three applications during the rainy season. It was used a complete random block experimental design with three factors being studied and two replications. In the experiment, four cows/paddock were used and, when it was necessary, regulator animals were added in order to obtain a supply of 7% body weight green forage dry matter. The animals were individually fed concentrate at 2 kg/day during the experimental period. Milk yield did not differ among the three grasses, with values of 9.1; 9.1; and 8.7 kg/cow/day for pastures with tanzania grass, star grass and marandu grass, respectively. Similarly, grass did not affect milk chemical composition. Stocking rate was similar among the three grasses, with values of 4.6; 4.5 and 5.0 UA/ha for tanzania grass, star grass and marandu grass, respectively. The highest dry matter intake was observed for tanzania grass with 2.6% of the body weight while stargrass (2.3%) and marandu grass (2.4%) did not differ among each other. The highest dry matter intake on tanzania grass pasture was not reflected on milk yield per animal. Milk yield and composition and stocking rate are similar among the evaluated grasses.

scholarly journals Health, body condition and blood metabolites in reindeer after submaintenance feed intake and subsequent feeding

Rangifer ◽  
2000 ◽  
Vol 20 (4) ◽  
pp. 187 ◽  
Author(s):  
A. Nilsson ◽  
Ö. Danell ◽  
M. Murphy ◽  
K. Olsson ◽  
B. Åhman
Keyword(s):  
Body Weight ◽  
Plasma Protein ◽  
Rangifer Tarandus ◽  
Plasma Concentrations ◽  
Experimental Period ◽  
Diet Group ◽  
The Body ◽  
Plasma Metabolites ◽  
Control Group ◽  
Feeding Period

The transition from experimentally induced poor nutritional conditions to feeding was studied with 69 eight-month-old female reindeer (Rangifer tarandus tarandus). During a pre-experimental period, all reindeer were fed a simulated winter diet with 80% lichens Cladina spp. and 20% Vaccinum myrtillus shrubs and Salix spp. leaves (lichen diet) ad lib. The reindeer were divided into five groups. A control group (group C) was fed the lichen diet ad lib. throughout the experiment. Four groups were fed half of that ration for eight days and were then totally deprived of feed for one day (restriction period). During the following 34 days (feeding period) the groups were re-fed the lichen diet (group L), fed pelleted reindeer feed combined with either lichen (group PL) or grass silage (group PS), or fed silage with a gradually increasing addition of pellets (group SP). Weekly measurements of blood samples and body weighr showed that the control group remained clinically healthy and had stable blood plasma concentrations of protein, urea, glucose and insulin throughout the experiment, but they lost weight. At slaughter, before and after the restriction period, all animals had lost rumen-free body weight, but the reindeer fed a restricted amount of feed lost more than the control group. Also the plasma metabolites were affected by the restricted feeding, with increased concentrations of urea and decreased concentrations of glucose. Group L responded immediately to the ad lib. feeding with blood metabolite levels rapidly approaching those of group C. The body weight developments were similar in groups L and C. Although the feed rations were increased gradually, diarrhoea occurred in some animals belonging to groups PL and PS within the first week of the feeding period. All reindeer recovered, after antibiotic treatment of the worst affected animals. The PL and PS groups, which had high contents of metabolisable energy and crude protein in their diets, showed increased con-centtations of plasma protein, urea and insulin. At the end of the feeding period, these groups had increased their body and carcass weights and gained fat, whereas reindeer fed the lichen diet had lost weight. Severe health problems (malnutrition and so-called wet belly) occurred in group SP during the first weeks of feeding and led to loss of animals, and consequently the SP group was excluded from the remainder of rhe experiment. The general conclusion is that the lichen diet did not cause any digestive problems, but resulted in a continuous decline in body weight and small or deficient fat reserves. After the initial diarrhoea, feeding with diets comprising pellets from the start resulted in improved condition, expressed as increased body weight, fat gain and higher concentrations of plasma protein, urea and insulin in relation to the control group. The diet initially based on grass in the form of silage of the given quality seemed insufficient as feed to reindeer calves in a poor nutritional state.

scholarly journals Oxidative Stress in Preterm Neonates: An Analysis of Oxidative Stress Biomarkers and Antioxidant Profiles

2020 ◽  
Author(s):  
Shobha S Pajai ◽  
Apurva P Bezalwar
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Cord Blood ◽  
Defense Mechanism ◽  
Preterm Neonates ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Study Results ◽  
Preterm Babies

Introduction: Oxidative stress is a complex event determined genetically and induced by an in- utero stressor. Oxidants are composed of reactive free radicals like Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) which are manifested by several macromolecules of lipid, protein and DNA, causing deleterious effects in several organs. Antioxidant defense mechanism and its ability to be induced by hyperoxia is relatively impaired in preterm neonates. Aim: To study oxidative stress and antioxidants in preterm neonates. Materials and Methods: This study is an observational analytical study, which included preterm babies (25 males and 20 females) delivered vaginally from October 2012 to October 2013. Cord blood was collected in citrate bulbs immediately after vaginal delivery and stored at 4°C until processed. Malondialdehyde (MDA), Nitrates, Vitamin C and Vitamin E, levels were measured in cord blood. Statistical z-test was applied. Results: High levels of oxidative stress biomarkers like MDA and Nitrites along with decreased levels of antioxidants, Vitamin C and Vitamin E in preterm neonates was observed. MDA and Nitrates levels were significantly higher in males (p<0.05) than females. Vitamin C and Vitamin E levels were not significant (p>0.05) in both. Conclusion: This study results may conclude that preterm neonates have more oxidative stress especially in males affecting their life survival.

Response of HepG2/C3A cells supplemented with sodium selenite to hydrogen peroxide-induced oxidative stress

2018 ◽  
Vol 50 ◽  
pp. 209-215 ◽  
Author(s):  
Thalita Alves Zanetti ◽  
Bruna Isabela Biazi ◽  
Adrivanio Baranoski ◽  
Gláucia Fernanda Rocha D’Epiro ◽  
Amanda Cristina Corveloni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Sodium Selenite

Butylated hydroxyanisole alleviates ferric nitrilotriacetate induced nephrotoxicity in rats by abrogation of oxidative stress

2013 ◽  
Vol 3 (2) ◽  
pp. 65-70
Author(s):  
Sabah Ansar ◽  
Mohammad Iqbal ◽  
Noura Al Jameil
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Body Weight ◽  
Antioxidant Enzymes ◽  
Control Group ◽  
Butylated Hydroxyanisole ◽  
Chemopreventive Agent ◽  
Microsomal Lipid Peroxidation ◽  
Hydrogen Peroxide Generation

In this study the effect of butylated hydroxyanisole (BHA), a phenolic antioxidantused in food on Ferric‐Nitrilotriacetate (Fe–NTA) induced nephrotoxicity is reported. Fe‐NTA (9 mg Fe/kg body weight, intraperitoneally) treatment enhanced the renal microsomal lipid peroxidation and hydrogen peroxide generation to ~2‐2.5 folds compared to saline‐treated control and glutathione levels and the activities of antioxidant enzymes decreased to a range of 2–2.5 fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHA. Pretreatment with BHA prior to Fe‐ NTA treatment reduced microsomal lipid peroxidation and hydrogen peroxide generation to 1.3‐1.5 fold compared to control group and glutathione and the activities of antioxidant enzymes increased to a range of 1.5‐2 folds in kidney. Fe‐NTA administration enhanced value of blood urea nitrogen and creatinine to 3.7 and 2.5 fold respectively as compared to their corresponding control group. Administration of Fe‐NTA to rats receiving a pretreatment of BHA led to a significant diminution in both of these values. The results indicate that BHA is a potent chemopreventive agent and suppresses Fe‐NTA induced nephrotoxicity in rats.

Maintenance rations for Merino sheep. IV. The performance of adult Merino ewes fed daily and weekly at three levels of energy intake

1957 ◽  
Vol 8 (1) ◽  
pp. 75 ◽  
Author(s):  
PK Briggs ◽  
MC Franklin ◽  
GL McClymont
Keyword(s):  
Body Weight ◽  
Sodium Chloride ◽  
Energy Intake ◽  
Survival Rates ◽  
Experimental Period ◽  
The Body ◽  
Merino Sheep ◽  
Wool Production ◽  
Body Weights ◽  
The Mean

Dry adult Merino ewes were fed at daily or weekly intervals on oat grain at levels which provided 4.0, 3.0, or 2.0 lb starch equivalent (S.E.) per sheep per week. The experimental periods were 223, 223, and 181 days respectively. Differences between the mean body weights of the ewes a t the three levels of feeding were highly significant (P < 0.001). Body weight varied only slightly and no losses occurred in ewes fed weekly at the rate of 4.0 lb S.E. per head. Ewes fed daily a t this level had a significantly greater mean body weight (P < 0.001) a t the conclusion of the 223-day experimental period. The addition of a sodium chloride supplement did not improve the body weight or wool production of ewes fed weekly a t the level of 4.0 lb S.E. The mean body weight of ewes fed at the levels of 3.0 or 2.0 lb S.E. per head declined over the first 12 and 18 respectively and thereafter remained relatively constant. There were no significant differences at these levels of feeding between groups fed daily and weekly in respect of body weight, wool production, or survival rates. Ewes fed a t the level of 4.0 lb S.E. grew significantly more wool than those given 3.0 lb S.E. (P < 0.001). Losses were negligible in all groups except those fed a t the level of 2.0 lb S.E. In these groups there were few deaths in the first 16 weeks, but in the subsequent 10 weeks losses totalled 17.1 per cent. Ewes fed a t the level of 2.0 lb S.E. consumed their rations at a significantly slower rate (P < 0.01) than did those fed a t the level of 4.0 lb S.E.

scholarly journals 127 Dietary supplementation with glycine improves the post-weaning growth of low-birth-weight pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 112-112
Author(s):  
Wenliang He ◽  
Erin A Posey ◽  
Guoyao Wu
Keyword(s):  
Body Weight ◽  
Birth Weight ◽  
Growth Rates ◽  
Corn Starch ◽  
Low Cost ◽  
Dietary Supplementation ◽  
Experimental Period ◽  
The Body ◽  
Economic Returns ◽  
On Farm

Abstract Pigs with intrauterine growth restriction (IUGR) represent 20–25% of all pigs born and are culled on farm, resulting in enormous losses. This study tested the hypothesis that dietary supplementation with glycine enhanced the growth of IUGR pigs after weaning. Healthy pigs [14 IUGR pigs (birth weight = 0.98±0.03 kg, mean ± SEM) and 20 NBW pigs (birth weight = 1.44±0.02 kg, mean ± SEM)] were used for the trial. At weaning (21 d of age), pigs within each birth weight group were assigned randomly into corn- and soybean meal-based diets supplemented with 1% glycine plus 0.19% corn starch or 1.19% alanine (isonitrogenous control). There were 7 IUGR pigs and 10 NBW pigs per subgroup. Crude protein content in basal diets was 20% between d 21 and 64, 18% between d 65 and 108, and 16% between d 109 and 120 of age. During the 100-d period of feeding, feed intake per kg body weight did not differ (P &gt; 0.05) between IUGR and NBW pigs or between control and glycine groups. Growth rates of NBW pigs supplemented with 1% glycine did not differ (P &gt; 0.05) from those for NBW pigs without glycine supplementation. In contrast, growth rates of IUGR pigs supplemented with 1% glycine were 28%, 15%, and 10% greater (P &gt; 0.05) than those for IUGR pigs without glycine supplementation during d 21–35, d 35–64, and d 65–120 of age, respectively. Growth rates of NBW pigs were greater (P &gt; 0.05) than those for IUGR pigs without glycine supplementation during any experimental period. By d 120 of age, the body weight of IUGR pigs with glycine supplementation did not differ (P &gt; 0.05) from that of NBW pigs. Collectively, our results indicate that dietary supplementation with 1% glycine (a low-cost supplement) beneficially improves their growth rate and economic returns. Supported by a USDA/NIFA grant.

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