scholarly journals Phytochemical screening and antibacterial activity of Cola nitida seed on selected bacterial isolates

2021 ◽  
Vol 14 (3) ◽  
pp. 001-007
Author(s):  
Dantani Dauda Odonye͙͙͙͙͙ ◽  
Peter Uteh Upla ◽  
Adegbe Lucy Ladi ◽  
Enoch Peter Odonye ◽  
Abisabo Adamu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Bacterial Species ◽  
Ethanol Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Bacterial Isolates ◽  
Zones Of Inhibition ◽  
Cola Nitida

Kola nut (Cola nitida) is chewed in many West African cultures and it is used ceremonially, industrially, medicinally and economically. The aim of this study was to assess the phytochemical properties and antibacterial activity of Cola nitida seeds. The seeds were purchased, dried and then powdered for phytochemical screening and extraction. Ethanol and aqueous (hot water) extracts were used for this study. The antibacterial activity of each extract at 30 mg/ml and 60 mg/ml was tested on four bacterial isolates; Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli using the disc diffusion method on Mueller Hinton agar. The results revealed the presence of various potent phytochemicals such as alkaloids, polyphenols, tannins, flavonoids and glycosides. The zones of inhibition ranged from 0.0 mm to 28.0 mm for both concentrations of the ethanol extract and from 0.0 mm to 31.0 mm for both concentrations of the aqueous extract. The Minimum Inhibitory Concentration (MIC) ranged from 7.5 mg/ml to 30.0 mg/ml for the ethanol extract and 3.75 mg/ml to 30.0 mg/ml for the aqueous extract. No minimum bactericidal concentration was recorded. Analysis of variance (ANOVA) performed on the data showed that there were significant differences in the zones of inhibition for each concentration of the extracts (P<0.05). The purified extract of Cola nitida seeds can be used to treat infections caused by these selected bacterial species.

IN VITRO EVALUATION OF ANISOPUS MANNII AND LEPTADENIA HASTATA FOR ANTIBACTERIAL POTENTIAL AGAINST BACTERIAL ISOLATES FROM DIABETIC WOUND

2021 ◽  
Vol 5 (2) ◽  
pp. 109-114
Author(s):  
Sakina B. Sani ◽  
B. M. Usman ◽  
M. Hayatu ◽  
A. Y. Hardiyya ◽  
A. Bilkisu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Diabetic Wound ◽  
Phytochemical Screening ◽  
Bacterial Isolates ◽  
Toxicological Effect ◽  
Diabetic Wound Healing ◽  
Healing Property

Leptadenia hastata and Anisopus mannii – are perennial plants of family Asclepiadaceae. They are widely distributed in West Africa and are locally used as anti-diabetic agents in Northern Nigeria. This study was conducted to investigate the phytochemical constituent and antibacterial activity of the crude ethanol extract of the Leptadenia hastata and Anisopus mannii against some bacterial isolates from diabetic wound. The phytochemical screening was carried out using standard protocol and antibacterial activity was determined by agar well diffusion method followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on the plant extract that showed activity. Result of phytochemical screening reveals the present of tannins, phenols, flavonols, saponins, and alkaloids in all the plants extract except in Anisopus mannii where alkaloid is absent. Also, result from antibacterial activity of leptadenia hastata demonstrated inhibition zones ranging from 11.5±0.71 to 20.0±0.00 at various concentrations (8mg/ml and 4mg/ml) against the tested organisms (Citrobacter specie, E.coli, Proteus vulgaris.) with MIC and MBC values ranging from 2mg/ml-8mg/ml. Hence, the ethanol extract of Leptadenia hastata can be considered as new therapeutic agent for the treatment of diabetic wound infection. Further studies need to be carried out to investigate toxicological effect and diabetic wound healing property of the plant.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals Evaluation of Antibacterial Activity of Zobo and Bay Leaf Extracts on Enteropathogenic Bacteria

2019 ◽  
pp. 1-7
Author(s):  
S. A. Wemedo ◽  
N. P. Akani ◽  
A. D. Amadiali
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Hot Water ◽  
Medical Laboratory ◽  
Alcoholic Extract ◽  
Mannitol Salt Agar ◽  
E Coli ◽  
Enteropathogenic Bacteria ◽  
Zones Of Inhibition

Aim: The antibacterial activity of Bay leaf (Laurus nobilis L.) and Zobo leaf (Hibiscus sabdariffa L.) extracts on enteropathogenic bacteria was investigated. Study Design: The study utilized well in agar diffusion to investigate the antimicrobial properties of the extracts. Place and Duration of Study: Department of Microbiology, Rivers State University and the study was carried out in August, 2018 to October, 2018. Methodology: Faecal samples were collected from a medical laboratory and inoculated on eosin methylene blue and mannitol salt agar plates for Escherichia coli and Staphylococcus aureus using standard microbiological techniques. The bacterial isolates were subjected to biochemical and molecular (PCR) identification so as to ascertain the distinctiveness of the isolates. Hot water and absolute alcohol were used as the extracting solvents. Concentrations of the extracted solvents was tested against E. coli and S. aureus using the well in agar method. Results: The result showed that both hot aqueous and alcoholic extracts of Bay leaf showed no sensitivity against the tested bacteria, whereas the extracts of hot dry aqueous and alcohol of Zobo leaf showed remarkable zones of inhibition against the tested bacteria. The zones of inhibition in the dry hot aqueous extract of zobo leaf with concentrations of 0.25 µg/mL, 0.125 µg/mL and 0.063 µg/mL were 31.3±0.1, 25.6±1.2 and 10.0±0.0, respectively. The minimal inhibitory concentration of the dry hot aqueous of zobo extract was observed at 0.063 µg/mL for E. coli, while zones of inhibition of 33.3±0.0, 30.1±0.3, 17.2±1.0 and 15.0±0.1 mm were recorded from the dry alcoholic extract of zobo leaf on E. coli given similar concentrations and the MIC was observed at the 0.031 µg/mL concentration. The result also showed that out of the four concentrations of the dry hot aqueous extract, only the 0.25 µg/mL concentration was able to show 14.2±0.0 mm inhibition on S. aureus, while the concentrations of 0.25 µg/ml and 0.125 µg/mL were the only two concentrations of the dry alcohol that showed levels of sensitivity with zone diameters of 29.3±1.0 and 25.2±0.0, respectively. Conclusion: The plant extracts of zobo leaves which displayed remarkable activity at fairly-low concentrations could be recommended for use against similar bacteria. Thus, investigation and adoption of plant extracts in modern medicine should be encouraged as this may be the break through needed to combat the ever-increasing resistance to commonly used antibiotics.

scholarly journals Evaluation of antibacterial efficacy of Ocimum sanctum L. against certain bacterial species and phytochemical analysis

2021 ◽  
Vol 22 (3) ◽  
pp. 341-345
Author(s):  
Aparajita Gupta ◽  
Mandeep Kaur
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Aqueous Extract ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Phytochemical Analysis ◽  
Antibacterial Efficacy ◽  
Minimum Value

In the present study antibacterial efficacy of  aqueous and methanol extract obtained from leaves and stem of Ocimum sanctum L. was evaluated against certain test bacterial species viz. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus cereus. It is a traditional medicinal herb, commonly used for the treatment of various human health disorders.  The antibacterial activity was determined by using agar well diffusion method. Among the solvent extracts tested, aqueous extract of leaves exhibited higher antibacterial activity as compared to methanolic extract of leaves. Maximum antibacterial activity was recorded 17.6 mm against Bacillus cereus and minimum value was recorded 14 mm against S. aureus. Maximum antibacterial efficacy of O. sanctum leaves was found 7.3mm against P. aeruginosa and minimum value was 5mm against B. cereus in methanolic extract. Similar trend has been observed in extract of stem of O. sanctum in aqueous and methanolic solvent. The antibacterial efficacy has been found nil in methanolic extract. However in aqueous extract moderate activity has been recorded i.e. 9.3 mm against E.coli and minimum 7.3 mm against K. pneumoniae. The trend of phytochemicals revealed non significant variation. Phytochemical analysis of leaves and stem extracts of O. sanctum revealed the presence of various phytoconstituents i.e. alkaloids, flavonoids, saponins, tanins, glycosides.

scholarly journals Potential Antibacterial Activity of Two Important Local Chewing Sticks “Fagara zanthoxyloides and Distemonanthus benthamianus” along with Antioxidant Capacities

2019 ◽  
Vol 18 (2) ◽  
pp. 223-232
Author(s):  
AO Shittu ◽  
A Aliyu ◽  
MS David ◽  
NS Njinga ◽  
HI Ishaq
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Zones Of Inhibition ◽  
Chewing Sticks

In order to authenticate and ascertain the various claim by the rural dwellers that depend on chewing sticks for their oral hygiene and protection against innumerable diseases, the antibacterial activity of the roots of Fagara zanthoxyloides and Distemonanthus benthamianus were evaluated. Preliminary phytochemical screening was carried out using standard methods. In-vitro antibacterial activity of the methanol extracts of both plants was also carried out using the agar well diffusion method against standard strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090 and also some clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis at different concentrations and ratios. Preliminary phytochemical screening showed that the extracts contained alkaloids, saponin, tannins, steroids, flavonoids, anthraquinones, cardiac glycosides and phenol. All the test microorganisms were susceptible to the inhibitory effect of the extracts at concentrations of 200 mg/ml and 500 mg/ml. F. zanthoxyloides had better antimicrobial activity with zones of inhibition ranging from 21.0 to 26.0 mm at 200 mg/ml and 24.3 to 29.3 mm at 500 mg/ml while D. benthamianus had zones of inhibition ranging from 17.7 to 26.7 mm at 200 mg/mL and 19.0 to 26.7 mm at 500 mg/mL. Some of the test microorganisms were resistant to the standard antibiotics (cefuroxime, ciprofloxacin, ofloxacin and gentamicin). Combinations of the extracts in ratios 50:50 and 75:25 yielded no increase in activity. The MIC and MBC for D. benthamianus ranged from 6.25 mg/ml to 100 mg/mL and 25 to 200 mg/ml, respectively while that of F. zanthoxyloides ranged from 1.56 mg/ml to 12.5 mg/mL and 50 to 200 mg/ml, respectively. The antimicrobial activity demonstrated by F. zanthoxyloides and D. benthamianus indicates that they would be valuable in the management of urinary, respiratory and gastrointestinal tracts infections while at the same time helping to mitigate the problem of antimicrobial resistance. Phenolic compounds of plants, among them flavonoids are the chief constituents, which have potent antioxidant activities Dhaka Univ. J. Pharm. Sci. 18(2): 223-232, 2019 (December)

scholarly journals In vitro antibacterial activity of Terminalia chebula leaf gall extracts against some human pathogenic strains

2012 ◽  
Vol 1 (8) ◽  
pp. 217-220 ◽  
Author(s):  
BE Ravi Shankara ◽  
YL Ramachandra ◽  
S Sundara Rajan ◽  
J Preetham ◽  
PS Sujan Ganapathy
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Terminalia Chebula ◽  
Bacterial Strains ◽  
South Indian

The leaf galls of Terminalia chebula is used widely as Karkatasringi in south Indian markets. Karkatashringi is an important crude drug employed in various indigenous systems of medicine against several diseases and the drug has diverse medicinal properties. The present study was carried out to understand the antimicrobial activity of various extracts. The antibacterial activity of T. chebula (leaf gall) was evaluated against ten bacterial strains including Gram-positive and Gram-negative bacteria using the agar-well diffusion method. Among the two extracts tested, the ethanol extract presented the best results against all the bacteria while aqueous extract showed moderate inhibition of the microbial growth. Each extract is unique against different microorganisms; Staphylococcus aureus was more susceptible to both extract among the tested organisms, whereas Serratia marcescens and Proteus mirabilis were less susceptible for ethanol and aqueous extract respectively. The inhibitory effect of the extracts was compared with standard antibiotic Ciprofloxacin.DOI: http://dx.doi.org/10.3329/icpj.v1i8.11254 International Current Pharmaceutical Journal 2012, 1(8): 217-220 

scholarly journals ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 8 (12) ◽  
pp. 262-269
Author(s):  
M. A. Garga ◽  
U. M. Garasin ◽  
M. Abdullahi ◽  
B. A. Muhammed ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Gram Staining ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BATANG MANGGA KWENI (Mangifera odorata Griff) TERHADAP Escherichia coli ATCC 25922 DAN Staphylococcus aureus ATCC 25923

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Wuri Prihatiningtiyas ◽  
Yeni Mariani ◽  
H A Oramahi ◽  
Fathul Yusro ◽  
Lolyta Sisilia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Positive Control ◽  
Plate Count ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Plate Count Agar

The aims of this research are to determine the secondary metabolite content found in the ethanol extract of kweni mango bark (Mangifera odorata Griff) and analyze its potency as a natural antibacterial against Escherichia coli and Staphylococcus aureus. The research was started by maceration process using 96% ethanol solvent, then evaporated at 40-50oC and obtain yield of 20,61% with powder content of 8,34%. Furthermore, phytochemical screening was performed qualitatively to determine the secondary metabolite of the extract. The results showed that ethanol extract of M. odorata Griff bark contained secondary metabolites such as alkaloids, flavonoids, saponins, tannins, terpenoids and phenolics. In this study antibacterial activity was carried out using disc diffusion method in Plate Count Agar media and incubated for 24-48 hours. The results showed that the largest diameter of inhibitory zones formed at a concentration of 15 mg/ml for S. aureus was 12,33 mm with strong classified and for E. coli bacteria with a concentration of 200 mg/ml of 23,67 mm with very strong classified, and almost equal with the result shown by 30 µg tetracycline as positive control, which is 25 mm. The results of this study it can be concluded that the ethanol extract of the kweni mango bark (M. odorata Griff) is bacteriostatic.Keywords: antibacterial activity, Mangifera odorata Griff, phytochemical screening, Escherichia coli, Staphylococcus aureus.

scholarly journals In Vitro Antibacterial Activity, Preliminary Phytochemical Screening Profile, and In Vivo Toxicity of Seven Traditional Medicinal Plants in Ethiopia

2022 ◽  
Author(s):  
Eyerus Mekuriaw ◽  
Enat Mengistu ◽  
Ayana Erdedo ◽  
Hassen Mamo
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Phytochemical Content

The threat of antibiotic-resistance calls for novel antibacterial agents. This study was aimed at screening medicinal plants for their antibacterial properties, phytochemical content and safety. Leaves of Allophylus abyssinicus (Hochst.) Radlk., Dicliptera laxata C.B.Clarke, Ligustrum vulgare L., Solanecio gigas (Vatke) c. Jeffrey and Gymnanthemum myrianthum (Hook.f.) H.Rob.; leaf and stem-bark of Olinia rochetiana A. Juss. and the seed of Cucurbita pepo L. were used. Chloroform and ethanol were used to extract G. myrianthum, D. laxata and O. rochetiana; ethyl acetate and methanol for the rest, and water for all. The extracts were tested against clinical/standard strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus by the agar-diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined. Acute toxicity to mice was checked and preliminary phytochemical screening was done. Thirteen extracts, out of 24, were active (inhibition zone >7 mm) at differing levels (9.67±0.33-25.66±0.57 mm) against at least one bacterial strain. The MICs and MBCs were 1.95-15.6 mg/mL and 7.8-125 mg/mL respectively. The aqueous extract of S. gigas, methanol extracts of L. vulgare and A. abyssinicus, and ethanol extract of O. rochetiana leaf were the most active (MIC 1.95mg/ml) against S. aureus. Ethyl acetate extracts of A. abyssinicus, L. vulgare and S. gigas; aqueous of C. pepo, O. rochetiana and G. myrianthum; and all D. laxata had no antibacterial activity. P. aeruginosa was the least susceptible to any extract, although the methanol and aqueous extracts of S. gigas performed better against it. Preliminary phytochemical screening of selected extracts for phenols, flavonoids, tannins, steroids, terpenoids, steroidal glycosides, alkaloids, saponins, resins and glycosides showed positivity at least for four of these phytochemicals with glycoside and terpenoids in nearly all extracts and resin in none. The plants were not toxic to mice at 2000 mg/kg. Further consideration of S. gigas, L. vulgare, A. abyssinicus and O. rochetiana is recommended in light of their promising potential and safety.

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