The Effect of Bakiko (Spinach- Chitosan- Collagen) Membrane on Total Fibroblast in Burn Wound Grade II

2018 ◽  
Vol 15 (2) ◽  
pp. 93
Author(s):  
SHOFI IQDA ISLAMI ◽  
AL MUNAWIR ◽  
IDA SRI SURANI WIJI ASTUTI
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Collagen Membrane ◽  
Fibroblast Cells ◽  
Burn Wound ◽  
Control Group Design ◽  
One Way Anova ◽  
Homogeneous Data ◽  
Second Degree

<pre><strong><em>Background:</em></strong><em> According to WHO data in 2016, every year, about 265,000 deaths occurred due to burns and 38% of burn patients got pathological scars due to delayed wound healing. Glutamine, vitamin C, and linoleic acid in spinach play an important role in wound healing by increasing the formation of granulation tissues including increasing the proliferation of fibroblasts. Chitosan-collagen dressings can also increase the formation of granulation tissue. Increased proliferation of fibroblasts will increase extra cellular matrix synthesis so that the wound heals quicker. The purpose of this research is to explain the effect of merging Spinach-Chitosan-Collagen (Bakiko) as membrane form on total fibroblas in healing process of second degree burns. </em></pre><pre><strong><em>Methods:</em></strong><em> This research is true experimental laboratories with posttest only control group design. The samples were 27 Rattus Novergicus divided into 9 groups: 3 negative control group (without therapy), 3 positive control group (Bioplacenton), and 3 treatment groups (Bakiko membrane). Second degree burns made by contacting the skin to hot metal plates. Bioplacenton was applied daily on wounds while Bakiko membrane is attached to the wound and replaced every 3 days. The tissue retrieval was done after the termination of the animals on the 3rd, 7th, and 21st days post-burn induction. The tissue was made as histopathologic preparations with HE staining then calculated the total fibroblast cells using imageJ software. Data analysis was done using One Way Anova for homogeneous data and Kruskal Wallis for non homogeneous data. </em></pre><pre><strong><em>Result:</em></strong><em> One Way Anova and Kruskal Wallis test  showed significant results on the 3rd day (p = 0,000) and 7th day (p = 0.004). </em></pre><strong><em>Conclussion:</em></strong><em> There is an effect of Bakiko membrane (spinach- chitosan- collagen) on the total fibroblasts in second degree burns by increasing the number of fibroblast cells at 3rd and 7th day on the proliferation phase of the burn-healing process.</em>

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Effect of haruan (channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 89
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals EFEK EKSTRAK TANAMAN ASHITABA PADA KADAR SERUM ALKALIN FOSFATASE (ALP) PADA TIKUS YANG TERPAPAR LUKA BAKAR

2021 ◽  
Vol 5 (1) ◽  
pp. 26-34
Author(s):  
Andrew Johan ◽  
Kusmiyati Tjahjono ◽  
Wenda Yoanda ◽  
Dwi Ngestiningsih ◽  
Lusiana Batubara
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Serum Alkaline Phosphatase ◽  
Healing Process ◽  
T Test ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Control Group Design

Serum alkaline phosphatase (ALP) levels increases in burn tissue damage. Several bioactive compounds found in Ashitaba, are expected to reduce serum ALP levels and enhance the process of wound healing. This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn trauma. This study uses true experimental post-test control group design with a total of 20 Sprague dawley rats as samples. All samples were inflicted with 2nd degree burn wound and divided into 2 groups, treatment group (Ashitaba extract 300 mg/KgBW) and control group. Blood serum were analyzed for ALP levels on the 2nd, 8th and 14th days. Kinetic-IFCC method was used to find serum ALP levels. Data was analyzed using Mann-Whitney Test, paired T-test amd independence T-test. Scalded wound size was measured macroscopically over the course of 21 days to find contracture rate. In conclusion, Ashitaba extract is not proven to significantly reduce the serum ALP levels, increase contracture rate and enhance burn wound healing process. However, there was slight increase in contracture rate in treatment group as compared to control group. In addition, there was a lower ALP levels in treatment group as compared to control group in day 8.

scholarly journals Healing Effect of Sesame Ointment on Second-degree Burn Wound in Rats

2016 ◽  
Vol 5 (2) ◽  
pp. 56-62
Author(s):  
Setareh Tehrani ◽  
Parisa Lotfi ◽  
Sepideh Tehrani ◽  
Ehsan Jangholi ◽  
Hoda Aryan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Sesame Oil ◽  
Control Group ◽  
Burn Wound ◽  
Matrix Deposition ◽  
Degree Burn ◽  
Anti Inflammatory ◽  
Second Degree

Background: Wound healing requires processes like cell migration, proliferation of extracellular matrix deposition, remodeling and angiogenesis. Also, anti-oxidative and anti-inflammatory reactions play key roles in the progression of wound healing. Previous studies showed that sesame oil has anti-inflammatory, antimicrobial and antioxidant activities. In addition, it is shown that sesame oil stimulates fibroblast production in vitro and in skin wounds. Therefore, in this study we investigated the effects of sesame ointment on the process of second-degree burn wound healing in rat models according to stereological parameters. Materials and Methods: In this experimental study, forty male Wistar rats (200±20 g) were randomly divided into four groups (n=10): ointment-base treated group (vehicle), silver sulfadiazine (SDD) treated group, sesame treated group (E1) and the control group which received no treatment. A 2×3 cm2 standard second-degree burn wound was induced on the posterior surface of animal’s neck under general anesthesia. After sacrifice, the animal’s skin sample was fixed in buffered formaldehyde for stereological evaluations. The data was analyzed by SPSS statistical software (version 14.0). P<0.05 was considered statistically significant. Results: According to our results, the mean of reduction in wound areas, volume density of collagen bundles and hair folicules, fibroblast populations, length density of vessels in E1 group was significantly higher than control group (P<0.05). The differences between E1 and SSD were not statistically noticeable regarding the stereological parameters. Conclusion: According to stereological analysis, administration of sesame ointment showed the ability to improve wound healing process and tissue regeneration in the treatment of skin damages.[GMJ.2016;5(2):56-62]

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing)

2020 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Desty Ayu Dwiyanti ◽  
Irham Taufiqurrahman ◽  
Didit Aspriyanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Group Design ◽  
Inflammatory Phase ◽  
Significant Difference

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Unsaturated Fat ◽  
Post Test ◽  
Channa Striata ◽  
The Mean

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

scholarly journals The Effect of Educated Platelets on the Healing Process of Burn Wounds in Rats: Educated Platelets in Rat Burn Wound Healing

2021 ◽  
Vol 8 ◽  
Author(s):  
Orkun Ilgen ◽  
Sefa Kurt ◽  
Osman Yilmaz ◽  
Murat Celiloglu
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Recovery Period ◽  
Control Group ◽  
Study Group ◽  
Second Line ◽  
Burn Wound ◽  
Wound Area ◽  
Burn Wound Healing

Burns are serious life-threatening health problems. This study was performed to determine the effect of educated platelets on burn wound healing process. 28 female wistar albino 200-220 gr rats were randomly divided into four groups. Group A1 rats(n:7) were the first-line burnt group from which blood samples are extracted to develop platelet-rich plasma(PRP) with the educated platelets that have a response to burn injury. Group B1 rats(n:7) were the unburnt group with ordinary platelets. Group A2 rats(n:8) were the second-line burnt group which was given PRP with educated platelets. Group B2 rats(n:6), as control group, were the second-line burnt group which was given PRP with ordinary platelets. Photos of rats' dorsum were taken by digital camera on the first day and 21st day of the study. Wound healing was determined by scar surface area. In the study group (Group A2) mean wound area was  53±37 mm², in the control group (Group B2) mean wound area was 114±55 mm² on the last day of the experiment. The sizes of the wounded areas were significantly lower in the study group compared with the control group (p: 0.039). Educated platelets seem to facilitate the recovery period of burn wound healing in rats.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

Sign in / Sign up

Export Citation Format

Share Document