scholarly journals The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts)

Sains Medika ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Ayyasi Izaz Almaz ◽  
Ratna Dama Purnawati ◽  
Hermawan Istiadi ◽  
Neni Susilaningsih
Keyword(s):  
Hydrogen Peroxide ◽  
Alternative Treatment ◽  
Wistar Rats ◽  
Vegf Expression ◽  
Group Design ◽  
Degree Burn ◽  
Group B ◽  
Post Hoc ◽  
Observation Results ◽  
Second Degree

Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats. Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given Bioplacenton�, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as Bioplacenton�

scholarly journals THE EFFECTIVENESS OF HYDROLYZED VCO CREAM ON VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) EXPRESSION AND COLLAGEN THICKNESS FOR HEALING 2ND DEGREE BURNS INJURY IN WISTAR RATS

2021 ◽  
Vol 8 (3) ◽  
pp. 273-280
Author(s):  
Fahmi Syarif ◽  
Neni Susilaningsih ◽  
Yuriz Bakhtiar
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Wistar Rats ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Burn Wounds ◽  
Degree Burn ◽  
Days Of Therapy ◽  
Endothelial Growth Factor ◽  
Second Degree

ABSTRACT    Burn wounds remain a serious problem in several countries. The presence of burn wounds might trigger local or in severe cases also triggers systemic response. Immediate treatment is required to prevent further tissue damage. Silver sulfadiazine cream is commonly used as a burn therapy. However, long-time usage might cause negative side effects. Several effective alternatives with better safety are opted, including virgin coconut oil (VCO). Originating from Cocos nucifera, commonly known as the coconut plant, VCO is a product that contains a lot of beneficial substances, such as lauric acid, polyphenol, and alpha-tocopherol. Hydrolyzed VCO can be an alternative to topical drugs in second-degree burns as it increases Vascular Endothelial Growth Factor (VEGF) expression and collagen thickness in the burn wound healing phase.    This randomized post-test only with parallel-group experiment was done using Wistar rats induced to a second-degree burn. The effect of 70% and 100% on second-degree burn healing, measured by hydrolyzed VCO on VEGF expression and collagen thickness was observed between 6 and 12 days of therapy. VEGF expression and collagen expression increased higher in groups treated with 70% and 100% hydrolyzed VCO compared with the control group treated with base cream.

scholarly journals Cyclophosphamide induced anemia in Wistar rats: can it be prevented by Sajna (Moringaoleifera)?

2020 ◽  
Vol 15 (1) ◽  
pp. 11-16
Author(s):  
Farhana Fatema ◽  
Sharmin Nahar ◽  
Qazi Shamima Akhter
Keyword(s):  
Wistar Rats ◽  
Dose Schedule ◽  
Free Access ◽  
Medical College ◽  
Before And After ◽  
The Mean ◽  
Level Of Significance ◽  
Group B ◽  
Access To Food ◽  
Post Hoc

Background: Chemotherapy-induced anemia is a multifactorial challenge to the treatment of malignancy. Herbal medicine may help totreat such anemia due to its natural compatibility. Moringaoleifera (Sajna), is well known for preventing and curing anemia. Objective: To evaluate the preventive effect of aqueous extract of Moringaoleiferaleaf (AEMOL) on cyclophosphamide (CP) induced anemia in Wistar rats. Method: The experimental study was carried out in the Department of Physiology, Dhaka Medical College, Dhaka from July 2015 to June 2016. Thirty Wistar ratsof both sexes weighing 175±25 gm were grouped as A[normal saline (1ml/kg); oral],and B [CP (3mg/kg);i. p.], C[AEMOL (300mg/kg);oral], D [AEMOL(300mg/kg); oral and CP(3 mg / kg) i. p. simultaneously for 14 consecutive days] and E [AEMOL(300mg/kg); oralfor 14 consecutive days and CP(30mg/ kg); i. p. on 12th, 13th,14th day] with 6 rats in each group.All the rats had free access to food and distilled water during the period of acclimatization (seven days) and experiment(fourteen days).Total count(TC)) of RBC, hemoglobin (Hb) level and packed cell volume (PCV)were estimated to assess anemia in all rats before and after experiment. The statistical analyses were done by one way ANOVA followed by Bonferroni post hoc test where p<0.05 was accepted as level of significance. Result: The mean TCof RBC, Hb% and PCV were significantly (p<0.001) lower in CP treated rats (group B), in comparison to all other rats of different groups (A, C, D and E). In addition, all these three variables were significantly higher in AEMOLtreated group(C) compared to saline treated rats (A)but almost similar in rats with two groups of different dose schedule of AEMOL and CP (D, E). Conclusion:From the study, it can be concluded that CP induced anemia can be prevented by AEMOL used either simultaneously or in an anticipating manner. J Bangladesh Soc Physiol. 2020, June; 15(1): 11-16

scholarly journals Positive Effects of Spray-Dried Streptococcus thermophilus on Healing of Second-Degree Burn Wounds in Wistar Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 23-29
Author(s):  
Amir Abbas Barzegari ◽  
Masood Hashemzaei ◽  
Ali Reza ASlihematti ◽  
◽  
◽  
...  
Keyword(s):  
Wistar Rats ◽  
Streptococcus Thermophilus ◽  
Burn Wounds ◽  
Positive Effects ◽  
Degree Burn ◽  
Spray Dried ◽  
Second Degree

scholarly journals Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Xiao-Feng Lin ◽  
Kai-Jie Chen ◽  
He-Kun Shi ◽  
Le Yu ◽  
Jin-Shan Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Wistar Rats ◽  
Molecular Mechanisms ◽  
Inflammatory Cells ◽  
Silver Sulfadiazine ◽  
Degree Burn ◽  
Immunohistochemical Assessment ◽  
Second Degree

Oxytropis falcata has long been used to treat inflammation, sores, and bleeding in Tibet. However, the burn remedy and underlying molecular mechanisms are not well understood. This study is aimed at assessing the effect of Oxytropis falcate gel (OFG) on deep second-degree burn rats and exploring its mechanism. Wistar rats with second-degree burn were treated with OFG and silver sulfadiazine. Immunohistochemical detections for EGF and VEGF were performed, and ELISA detections for EGF, VEGF, p38, and IL-1β in serum were determined. Rats treated with OFG (25, 50 g/kg) consisted of the major rhamnocitrin-3-O-β-neohesperidoside significantly accelerated incrustation (P<0.001) and decrustation (P<0.001). According to HE staining, edema and infiltration of inflammatory cells decrease apparently with good hyperplasia and incrustation in administration groups (7 d). The expressions of EGF and CD34 in OFG (25, 50 g/kg) treatment increased obviously from immunohistochemical assessment (7 d). Serum EGF expression reached 321.27±7.20 ng/mL by OFG treatment, while p38 (P<0.05) and IL-1β (P<0.05) levels were significantly lower than the model and vehicle groups from day 1 to day 7. OFG possesses potential wound healing activities. The mechanism may be related to the increasing of biosynthesis and the releasing of EGF and CD34 and the decreasing p38 and IL-1β levels.

scholarly journals Management of walled-off necrosis with nasocystic irrigation with hydrogen peroxide versus biflanged metal stent: randomized controlled trial

2021 ◽  
Vol 09 (07) ◽  
pp. E1108-E1115
Author(s):  
Sudhir Maharshi ◽  
Shyam Sunder Sharma ◽  
Sandeep Ratra ◽  
Bharat Sapra ◽  
Dhruv Sharma
Keyword(s):  
Hydrogen Peroxide ◽  
Adverse Events ◽  
Clinical Success ◽  
Controlled Trial ◽  
Procedure Time ◽  
Metal Stent ◽  
Technical Success ◽  
Group A ◽  
Group B ◽  
Walled Off Necrosis

Abstract Background and study aims Walled-off necrosis (WON) is a known complication of acute necrotizing pancreatitis (ANP). There is no study comparing nasocystic irrigation with hydrogen peroxide (H2O2) versus biflanged metal stent (BMS) in the management of WON. The aim of this study was to compare the clinical efficacy of both the treatment strategies. Patients and methods This study was conducted on patients with symptomatic WON who were randomized to nasocystic irrigation with H2O2 (Group A) and BMS placement (Group B). Primary outcomes were clinical and technical success while secondary outcomes were procedure time, adverse events, need for additional procedures, duration of hospitalization, and mortality. Results Fifty patients were randomized into two groups. Group A (n = 25, age 37.8 ± 17.6 years, 16 men) and Group B (n = 25, age 41.8 ± 15.2 years, 17 men). There were no significant differences in baseline characteristics between the two groups. The most common etiology of pancreatitis was alcohol, observed in 27 (54 %) patients. Technical success (100 % vs 96 %, P = 0.98), clinical success (84 % vs 76 %, P = 0.76), requirement of additional procedures (16 % vs 24 %, P = 0.70) and adverse events (4 vs 7, P = 0.06) were comparable in both the groups. The duration to clinical success (34.4 ± 12 vs 14.8 ± 10.8 days, P = 0.001) and procedure time (36 ± 15 vs 18 ± 12 minutes, P = 0.01) were longer in Group A compared to Group B. Conclusions Nasocystic irrigation with H2O2 and BMS are equally effective in the management of WON but time to clinical success and procedure time is longer with nasocystic irrigation.

scholarly journals Investigating the Impact of Collagen-Chitosan Derived from Scomberomorus Guttatus and Shrimp Skin on Second-Degree Burn in Rats Model

2021 ◽  
Vol 18 ◽  
pp. 12-20
Author(s):  
Mohammad Javad Fatemi ◽  
Soheila Naderi Garahgheshlagh ◽  
Tayyeb Ghadimi ◽  
Shahla Jamili ◽  
Mohammad Reza Nourani ◽  
...  
Keyword(s):  
Degree Burn ◽  
The Impact ◽  
Second Degree

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

Wound Healing Activity of Avocado Peel Ointment against Second Degree Burn Wound

2021 ◽  
Author(s):  
Ma I Yang ◽  
Ermi Girsang ◽  
Ali Napiah Nasution ◽  
Chrismis Novalinda Ginting
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Degree Burn ◽  
Wound Healing Activity ◽  
Second Degree

scholarly journals The effects of Roux-en-Y limb length on gastric emptying and enterogastric reflux in rats

2008 ◽  
Vol 23 (2) ◽  
pp. 179-183 ◽  
Author(s):  
Robson Azevedo Dutra ◽  
Wemberton Martins Araújo ◽  
José Ivan de Andrade
Keyword(s):  
Gastric Emptying ◽  
Wistar Rats ◽  
Limb Length ◽  
Roux Limb ◽  
Short Limb ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B ◽  
Enterogastric Reflux

PURPOSE: To assess the effects of Roux-en-Y jejunal limb length on gastric emptying and enterogastric reflux. METHODS: Seventy male Wistar rats were submitted to antrectomy with Roux-en-Y reconstruction and then were divided into two groups of 35 animals. Group A, short limb (7.5 cm) and Group B, standard limb (15 cm). Group A and B were subdivided into five subgroups each in order to study enterogastric reflux at 30 and 60 minutes and to evaluate gastric emptying at 5, 10 and 15 minutes. In order to measure gastric emptying and enterogastric reflux, radiotracers 99m Tc-Phytate and 99m Tc-DISIDA were respectively used. RESULTS: For gastric emptying, the radiotracer concentration was lower in Group A than in Group B after five minutes. The enterogastric reflux was present, but there were no significant differences between enterogastric reflux indexes concerning both A and B Groups. CONCLUSION: A standard Roux limb, besides being unable to protect the stomach from the enterogastric reflux, may become a functional barrier for gastric emptying.

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