EFFECTS OF DIFFERENT CONCENTRATIONS OF TITANIUM OXIDE NANOPARTICLES AND BULK ON GERMINATION CHARACTERISTICS AND ANTIOXIDANT ENZYMES ACTIVITY OF AVENA SATIVA L. SEEDS

2021 ◽  
Vol 24 (1) ◽  
Author(s):  
Moein Iranmanesh ◽  
◽  
Abolfazl Beyraghdar ◽  
Homa Mahmoodzadeh ◽  
◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Titanium Oxide ◽  
Germination Percentage ◽  
Shoot Length ◽  
Guaiacol Peroxidase ◽  
Seedling Vigor ◽  
Titanium Oxide Nanoparticles ◽  
Vigor Index ◽  
Germination Characteristics ◽  
Peroxidase Enzyme

The aim of this study is to compare the effects of different concentrations of nano and bulk titanium oxide on germination characteristics and the activity of antioxidant enzymes in Avena Sativa L. The titanium oxide nanoparticles diameter was determined about 40 nm using AFM, DLS, XRD analysis. In this study, based on a completely randomized design, seeds were placed in Petri dishes containing different concentrations of nano and bulk titanium oxide (50, 150, 250, 350 ppm). Three replications were considered for each concentration and the germination factors and antioxidant enzyme activity in the treated plant were compared with the control. The average data were compared using one-way ANOVA analysis and DUNCAN tests. According to the results, the following parameters of seedling vigor index, root length, dry weight, wet weight, guaiacol peroxidase enzyme, and polyphenol oxidase were statistically significant. The highest germination percentage, relative germination percentage, germination rate, weight germination index and shoot length were observed at 250 ppm bulk titanium oxide. In addition, the highest average germination time, seedling vigor index, optimum seedling index, and root length were observed in 50 ppm bulk titanium oxide treatment and the highest shoot length was observed in 250 ppm nano-titanium oxide treatment. In the enzyme activity, the highest activity of guaiacol peroxidase enzyme was observed in 150 ppm bulk titanium oxide treatment and the highest Polyphenol oxidase enzyme activity was observed in bulk titanium oxide 350 ppm treatment.

scholarly journals Improvement of Germination Characteristics and Enhancement of Antioxidant Enzymes Activity of Safflower (Carthamus tinctorius L.) Aged Seeds by Used of Gibberellin

2015 ◽  
Vol 48 (3) ◽  
pp. 33-41
Author(s):  
M. Khajeh ◽  
S.A. Tabatabaei ◽  
O. Ansari ◽  
F. Sharif Zadeh
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Ascorbate Peroxidase ◽  
Seed Priming ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Characteristics ◽  
Normal Seedling

AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.

scholarly journals Enhancement of rice and wheat seed germination and seedling vigor by biocontrol agent, Cladosporium cladosporioides

2020 ◽  
Vol 7 (1) ◽  
pp. 9-15
Author(s):  
Md Touhidul Islam
Keyword(s):  
Seed Germination ◽  
Biocontrol Agent ◽  
Germination Percentage ◽  
Cladosporium Cladosporioides ◽  
Germination Index ◽  
Rice Seed ◽  
Seedling Vigor ◽  
Wheat Seed ◽  
Mean Values ◽  
Vigor Index

The biocontrol agent, Cladosporium cladosporioides, strain BOU1 was used to evaluate the rice and wheat plant growth by germination percentage, germination index, vigor index- I and vigor index-II in rice and wheat seed. All the variables of germination and seedling vigor of rice and wheat were increased by the conidial suspension of C. cladosporioides. The rice seed treated with C. cladosporioides had the highest germination index and vigor index-I with average mean values of 8.2 and 4.5, respectively; while the treated wheat seed showed the highest germination percentage and vigor index- II with average mean values of 80.3 and 0.05, respectively. The research is concluded that the biocontrol agent C. cladosporioides improves seed germination and seedling vigor of wheat and rice, which can finally be converted into superior yield even in adverse conditions. Res. Agric., Livest. Fish.7(1): 09-15, April 2020

Evaluation of Herbicidal Potential of Eucalyptus spp for Biological Control of Dactyloctenium aegyptium L.

2021 ◽  
Vol 20 (1) ◽  
pp. 186-191
Author(s):  
Amal Ehtaiwesh
Keyword(s):  
Foliar Application ◽  
Growth Traits ◽  
Negative Impact ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Shoot Length ◽  
Dactyloctenium Aegyptium ◽  
Vigor Index ◽  
Root And Shoot Length ◽  
Germination Traits

Pots culture experiment was conducted to evaluates the herbicidal potential of Eucalyptus spp. (aceae) on growth of Dactyloctenium aegyptium L. (Poaceae). Leaves extract with concentrations of 10, 20 and 40% of Eucalyptus plant were applied as soil and foliar application to seeds and seedlings of D. aegyptium species.  The result indicated that the application of Eucalyptus leaves extracts caused significant reduction in germination traits, root and shoot length, seedling fresh and dry weights. Also, the result revealed that the Inhibitory effect of Eucalyptus extract on D. aegyptium L was more noticeable in plant growth traits than on germination traits. Results show that among the different concentration of Eucalyptus leave extracts used in this study, 40% was the most toxic and caused the significant effect on germination and growth traits of Dactyloctenium aegyptium. Application of 40% of Eucalyptus leave extract decreased germination percentage by 30%, mean daily germination by 45%, shoot length by 38%. root length by 54%, number of branches by 61%, number of leavesby59%, seedling fresh weights by 23%, seedling dry weights by 62% and seedling vigor index by 60%. The results of this study concluded that the use of Eucalyptus plants have the potential to be developed further as a bio-herbicide system to control weed such as Dactyloctenium aegyptium L. However, more researches are needed to evaluate the negative impact of Eucalyptus on crop growth.

scholarly journals Rice (Oryza sativa L.) Seed Priming: It’s Effect on Seed Germination and Seedling Emergence of Spring Varieties in Kapilvastu District, Nepal

2021 ◽  
Vol 8 (2) ◽  
pp. 196-201
Author(s):  
Sabita Ghimire ◽  
Hariom Yadav ◽  
Prabesh Acharya ◽  
Raju Kharel
Keyword(s):  
Vitamin C ◽  
Root Length ◽  
Oryza Sativa L ◽  
Tap Water ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Length ◽  
Superior Performance ◽  
Vigor Index

This study aimed to determine the effect of seed priming (on-farm, hydro, halo, osmo, vitamin c priming, and hardening) on the germination and seedling growth of spring rice varieties: hardinath-3, hardinath-1, and chaite-5 at ambient room temperature. A factorial completely randomized design (CRD) was carried out with two factors: methods of priming and varieties at agriculture knowledge center (AKC), kapilbastu during march 2020. There were, in total, 21 treatment combinations and four replications. Parameters observed were germination percentage, speed of germination, vigor index, root length, shoot length, fresh weight, and dry weight of rice seedlings. Hardinath-3 had superior performance in terms of germination percentage (95.35 %), vigor index (183.86), root length (11.51 cm) and shoot length (7.39 cm) compared to hardinath-1 and chaite-5; the speed of germination, however, was greater in hardinath-1. Seed hardening - alternate soaking (tap water for 24 hours) and drying - induced higher germination percentage (94.91 %), speed of germination (96.00), and vigor index (197.43) than did other priming methods; germination percentage of vitamin c primed seeds (94.75 %), nevertheless, were at statistical par with hardened seeds. Hardened hardinath-3 seeds were better in regards to germination percentage (97.50 %) and root length (12.92 cm), while the speed of germination (97.50) and vigor index (210.50) was greater in hardened hardinath-1. Vitamin c primed seeds of hardinath-3 and hardinath-1 were finer with dry weight, shoot length, and vigor index. In conclusion, farmers' are suggested to hardened seeds of hardinath-3 before sowing to promote uniform germination and growth.

scholarly journals Impact of seed size on seeds viability, vigor and storability of Hordeum vulgare (L.)

2018 ◽  
Author(s):  
M. Massimi
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Hordeum Vulgare L ◽  
Vigor Index ◽  
The Impact

Seed size may influence seed germination, and seedling vigor. Few investigations are available about the effect of seed size on barley seedling vigor in Jordan. The present study was designed to investigate the impact of seed size on germination percentage, seedling dry weight, seedling vigor index, and germination percentage after accelerated aging in barley. Three seed sizes i.e. large, medium, and small (having diameter of > 2.75 millimeter, 2.5 - 2.75 millimeter, and less than 2.5 millimeter, respectively) were tested in the experiment. Seed quality for different seed size categories was evaluated in the laboratory by measuring seed germination, germination after accelerated aging, as well seedling dry weight and vigor index. Results showed significant differences for large seeds in germination percentages, seedling dry weight and vigor index. It may be concluded that large seed size of barley showed best quality.

scholarly journals Temporal variation in seed quality of Indian spinach preserved in different containers

2016 ◽  
Vol 5 (2) ◽  
pp. 51-57 ◽  
Author(s):  
M Kamruzzaman ◽  
S Khatun ◽  
A Rakib ◽  
MI Hoque ◽  
MH Rani
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Fresh Weight ◽  
Seed Technology ◽  
Vigor Index

The experiment was conducted to investigate the seed quality of Indian spinach for three months stored at different containers (Plastic pot, polythene bag and cloth bag) during 2013-2014 at Seed Technology Laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur. The initial moisture content of the seed was 9.89% with 75.00% germination. However, after two months, the seed had a different range of increased moisture content with reduced germination percentage depending upon the packing materials. Among the three containers, seeds stored at cloth bag absorbed more moisture (14.36%) from surrounding atmosphere followed by the moisture content, 12.67% and 11.50% of polythene bag and airtight plastic pot, respectively. Seeds stored at plastic pot, polythene bag and cloth bag exhibited 65.00, 64.00 and 50.00 per cent germination, respectively. Similarly, the fresh weight and dry weight of seedling and seedling vigor were also decreased. The seeds of plastic pot were good in term of germination capacity, fresh and dry weight and vigor index in comparison to those stored at cloth bag and polythene bag.Int. J. Agril. Res. Innov. & Tech. 5 (2): 51-57, December, 2015

scholarly journals PENGARUH PERENDAMAN TERHADAP VIABILITAS BENIH TEMBAKAU (Nicotiana tabacum L.)

2020 ◽  
Vol 20 (2) ◽  
pp. 87
Author(s):  
SIWI SUMARTINI ◽  
SRI MULYANI ◽  
FATHKUR ROCHMAN
Keyword(s):  
Nicotiana Tabacum ◽  
Root Length ◽  
Seed Viability ◽  
Germination Percentage ◽  
Test Method ◽  
Seedling Vigor ◽  
Tobacco Seed ◽  
Vigor Index ◽  
Nicotiana Tabacum L ◽  
Tobacco Seeds

<p>ABSTRAK<br />Permasalahan dalam pengembangan tembakau rakyat adalah daya<br />berkecambah benih yang rendah. Penelitian ini bertujuan untuk<br />mengetahui pengaruh perendaman benih terhadap daya berkecambah<br />benih tembakau (Nicotiana tabacum L.). Penelitian dilaksanakan di<br />laboratorium Balai Penelitian Tanaman Pemanis dan Serat pada bulan Mei<br />sampai dengan Juli 2013. Perlakuan disusun dalam rancangan petak<br />terbagi dan diulang empat kali. Sebagai petak utama adalah tujuh varietas<br />tembakau lokal, yaitu V1 = Kemloko1; V2 = Kemloko2; V3 = Kemloko3;<br />V4 = Kasturi1; V5 = Kasturi2; V6 = Grompol Jatim1; dan V7 =<br />Bojonegoro1. Sebagai anak petak adalah: R1 = Tanpa perendaman<br />(kontrol); R2 = perendaman selama satu jam dalam air; R3 = perendaman<br />selama satu jam dalam larutan KNO 3  (0,1%), dan R4 = perendaman<br />selama satu jam dalam larutan KNO 3 (0,2%). Setelah perlakuan<br />perendaman, benih tembakau dikecambahkan menggunakan metode Uji di<br />Atas Kertas. Pada setiap ulangan, sebanyak 100 benih tembakau<br />dikecambahkan pada media kertas merang yang diletakkan di dalam<br />petridish berdiameter 9 cm. Perkecambahan dilakukan di dalam<br />germinator tipe IPB dengan suhu 23 o C dan kelembaban nisbi 87-93%.<br />Parameter yang diamati adalah daya berkecambah, panjang kecambah,<br />panjang akar kecambah, dan indeks vigor kecambah. Perendaman benih<br />tembakau menggunakan air, larutan KNO 3 0,1% dan larutan KNO 3 0,2%<br />selama satu jam sebelum benih disemaikan, dapat meningkatkan daya<br />berkecambah dan panjang kecambah varietas Kemloko1 dan Grompol<br />Jatim1. Perlakuan perendaman benih dengan air berpengaruh positif pada<br />varietas Kemloko1 yang ditunjukkan dengan daya berkecambah tertinggi,<br />sedangkan perendaman dengan larutan KNO 3 0,2% berpengaruh negatif<br />pada varietas Bojonegoro1 yang ditunjukkan dengan daya berkecambah<br />paling rendah. Perlakuan perendaman dengan air maupun larutan KNO 3<br />(0,1% dan 0,2%) menunjukkan pengaruh yang berbeda-beda terhadap<br />parameter daya berkecambah, panjang kecambah, panjang akar kecambah,<br />dan indeks vigor kecambah pada semua varietas tembakau yang diuji.<br />Kata kunci: Nicotiana tabacum L., perendaman, KNO 3 , viabilitas benih</p><p>ABSTRACT<br />Low germinability of seeds is one of major problems in tobacco<br />development. The aim of this study was to determine the effect of priming<br />on tobacco (Nicotiana tabacum L.) seed viability. The research was<br />conducted in the laboratory of the Indonesian Sweeteners and Fiber Crops<br />Research Institute during May to July 2013. The treatments were arranged<br />in a split plot design with four times of replication. The main plots were<br />seven tobacco varieties namely V1 = Kemloko1; V2 = Kemloko2; V3 =<br />Kemloko3; V4 = Kasturi1; V5 = Kasturi2; V6 = Grompol Jatim1; and V7<br />= Bojonegoro1. The subplots were priming seeds for one hour namely R1<br />= without priming (control); R2 = priming for one hour on water; R3 =<br />priming for one hour on KNO 3  (0,1%) solution, and R4 = priming for one<br />hour on KNO 3 (0,2%) solution. After priming, seeds were germinated using<br />the Upper Paper Test method. A hundred of seeds were sown on straw<br />paper media in a petridish diameter 9 cm of each replication. Parameters<br />measured were germination percentage, shoot and root length, and<br />seedling vigor index. Priming tobacco seed with water or KNO 3  (0.1 and<br />0.2%) solution for one hour before seeds were germinated significantly<br />improved germination percentage and shoot length of Kemloko1 and<br />Grompol Jatim1 varieties. Priming tobacco seed with water had positive<br />effect on Kemloko1 variety which resulted the highest germination<br />percentage but had adversely effect on Bojonegoro1 variety which resulted<br />the lowest germination percentage. Priming tobacco seeds with water or<br />KNO 3 (0.1 and 0.2%) solution resulted different effect on germination<br />percentage, shoot and root length, and seedling vigor index parameters for<br />all tobacco varieties were observed.<br />Key words: Nicotiana tabacum L., priming, KNO 3 , seed viability</p>

scholarly journals Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 516 ◽  
Author(s):  
Mihaiela Cornea-Cipcigan ◽  
Doru Pamfil ◽  
Cristian Radu Sisea ◽  
Rodica Mărgăoan
Keyword(s):  
Gibberellic Acid ◽  
Ornamental Plants ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Higher Plant ◽  
Vigor Index ◽  
Mean Germination Time ◽  
Number Of Leaves ◽  
Ornamental Crops

Cyclamen species (swinebread spp.) are perennial herbaceous plants which belong to the Primulaceae family, widely used as medicinal or ornamental plants. Currently, gibberellic acid (GA3), an environmentally friendly bioregulator is widely used to enhance the productivity and phenotypic characteristics of multiple ornamental crops. A dual experiment was conducted to evaluate the influence of gibberellic acid (50, 100 or 150 mg/L), on germination earliness of seeds, along with growth and ornamental quality of selected Cyclamen species. Additionally, a greenhouse experiment with long and short sunlight photoperiod was conducted to evaluate its importance in the development of Cyclamen plants. Our results indicated that under short sunlight exposure, seeds germinated earlier than those kept in long sunlight photoperiod. Plants treated with foliar applications of GA3 exhibited higher plant height, leaf area, number of leaves, root length and tuber development. Significant differences were found in germination percentage (GP), mean germination time (MGT) and seedling vigor index (SVI), within different GA3 supplementation and greenhouse sunlight intensity (short and long days). In conclusion, our study suggests that seedling vigor and ornamental quality can be increased by GA3 application, an economic strategy for enhancing germination and growth of selected Cyclamen species.

scholarly journals Effect of osmopriming on rice seed germination and seedling growth

2012 ◽  
Vol 10 (1) ◽  
pp. 15-20 ◽  
Author(s):  
R Islam ◽  
A Mukherjee ◽  
M Hossin
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Shoot Length ◽  
Rice Seed ◽  
Randomized Design ◽  
Vigor Index ◽  
Two Factors ◽  
Treated Seed ◽  
Germination Parameters

The experiment was conducted in the Agronomy Laboratory of Agrotechnology Discipline, Khulna University, Khulna from 29th August to 17th September, 2009. The experiment was laid out in a Completely Randomized Design (CRD) with two factors and three replications. The aim of the experiment was to study the efficacy of three osmopriming agents viz ., NaCl, KC1 and CaCb with control on seed germination and seedling growth of three T. aman rice varities viz., BRRI dhan40, BRRI dhan41 and BINA dhan7. Among the chemicals CaCb showed best as osmopriming agents and it enhanced all germination parameters. BRRI dhan41 showed the best performance. Better root length and shoot length were observed in control. Seeds of BRRI dhan41 when treated with NaCl showed the highest germination percentage (98.67%), germination energy (88%), germination speed (96.83%), but vigor index (10.63) was found highest in BRRI dhan40 when it was treated with KC1. BRRI dhan41 produced the largest root (8.56 cm) when seeds were treated with NaCl. BRRI dhan40 (10.51 cm) and BINA dhan7 (10.53 cm) produced the highest shoot length at controlled condition and CaCl2 treated seed, respectively.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12013   J. Bangladesh Agril. Univ. 10(1): 15–20, 2012  

scholarly journals Cryopreservation of Arachis Hypogaea L. Varieties, from the INIAP-Ecuador Germplasm Bank

2021 ◽  
Author(s):  
Marcelo Tacán ◽  
César Tapia ◽  
César Pérez ◽  
Eddie Zambrano ◽  
Alma Mendoza ◽  
...  
Keyword(s):  
Arachis Hypogaea ◽  
Root Formation ◽  
Germination Percentage ◽  
Shoot Length ◽  
Control Treatment ◽  
Good Survival ◽  
Seedling Vigor ◽  
Germplasm Bank ◽  
Embryonic Axes ◽  
Arachis Hypogaea L

The peanut (Arachis hypogaea L.) is recognized as one of the most important legume crops globally for its use in human food; it is widely distributed and cultivated in tropical and subtropical regions. The purpose of this study was to evaluate the cryopreservation of five peanut varieties conserved in the INIAP Germplasm Bank, testing cryopreservation methods, evaluating the germination percentage of whole seeds and embryonic shoots. Subsequently, two quantitative variables, shoot length and root, were evaluated. The average germination percentage of varieties and treatments was higher when embryonic axes were isolated with 99.31% than 86.06% seeds. The best germination percentage of the five varieties for seeds and embryonic shoots was obtained by the Peruvian variety with 88.13% and 92.50%. The best treatments by variety for the germination of whole seeds and embryonic axes were obtained by the treatment (desiccation and NL) for whole seeds (GS2) with 95.42% and embryonic axes with 92.83%. Ageing and cryopreservation treatments positively affected germination and seedling vigor in whole seeds and embryonic axes. The two quantitative variables, shoot and root length showed variability between the five varieties; significant differences were observed between the four treatments evaluated for whole seeds and embryonic axes. The three treatments for whole seeds (GS1, GS2 GS3) and the non-cryopreserved control treatment (GSC), as well as the treatments for embryonic axes (GEA1, GEA2 GEA3) and the non-cryopreserved control treatment (GEAC), obtained good survival. They germinate whole seeds and embryonic axes with sprout development (aerial part) and root formation. With the most effective treatments for whole seeds (GS2) and embryonic axes (GEA2), the cryopreservation of the national peanut collection of the INIAP Germplasm Bank could be started.

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