scholarly journals Changes in behavioural and locomotory activities of freshwater fish, Cirrhinus mrigala (Hamilton) in response to sublethal exposure of Chlorpyrifos

2018 ◽  
Vol 10 (2) ◽  
pp. 620-626
Author(s):  
Navneet Cheema ◽  
Anita Bhatnagar ◽  
Abhay Singh Yadav
Keyword(s):  
Acute Toxicity ◽  
Beat Frequency ◽  
Probit Analysis ◽  
Cirrhinus Mrigala ◽  
Pollution Management ◽  
Avoidance Tests ◽  
The Impact ◽  
Tail Beat ◽  
Sublethal Concentrations ◽  
Fish Avoidance

The aim of the present study was to analyze the impact of sub-lethal toxicity of chlorpyrifos, one of the largest market selling agrochemical on physiological parameters of teleost fish, Cirrhinus mrigala addressing the possible causative involvement in behavioural and locomotion responses. Primarily, the acute toxicity (96h LC50) test was carried out and the value calculated by probit analysis was found to be 0.44 mg L-1. Further, one-fifth, one-tenth and one-twentieth of 96 h LC50 were selected as sublethal concentrations for sub acute studies. The experiment was carried out for 21 days and the alternative behaviour was recorded in terms of Air Ingulping (AI), Operculum Beat Frequency (OBF), Surfacing Movement (SM), Vertical Hanging (VH) and Tail Beat Frequency (TBF) on duration day 2, 4, 7, 14 and 21. Significant effect of both the concentrations and duration was observed in fishes treated with selected doses of chlorpyrifos. It was found that AI, OBF, SM, VH, TBF was highest on day 7 at 0.08 mg L-1 concentration of Chlorpyrifos. The findings revealed that there is a need to control the use of chlorpyrifos because of its toxicity. All the fish avoidance tests proved to be an important predictive and sensitive biomarker in aquatic monitoring and pollution management.

scholarly journals Temperature changes alter the acute toxicity responses of cypermethrin in Zebrafish

2018 ◽  
Vol 29 (1) ◽  
pp. 64-70 ◽  
Author(s):  
MH Uddin ◽  
MS Alim ◽  
SMM Islam ◽  
H Rashid ◽  
M Shahjahan
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Probit Analysis ◽  
Effect Of Temperature ◽  
Temperature Changes ◽  
Temperature Regimes ◽  
Higher Temperature ◽  
T1 And T2 ◽  
Increasing Temperature ◽  
The Impact

The study was carried out to determine the effect of temperature changes on acute toxicity of pyrethroid pesticide cypermethrin in zebrafish. A two-day renewal bioassay system for 96h was conducted to find out LC50 value of cypermethrin at two temperature regimes i.e. 25°C and 30°C considering as T1 and T2, respectively. During the determination of LC50 in both temperatures, blood glucose (mg/dL) levels were measured at lower concentration (0.25 µg/L) of cypermethrin. The results of acute toxicity test at 96h LC50 values were calculated through probit analysis. It was found that 96h LC50 for T1 and T2 groups were about 2.1 and 1.4 µg/L, respectively. Significantly lower LC50 of cypermethrin at T2 compared to T1 showed that higher temperature increased the toxicity of cypermethrin. There was a significant increase (P<0.05) in blood glucose level (mg/dL) in 0.25 µg/L compared to 0 µg/L concentration of cypermethrin at both treatments. Dissolved oxygen decreased and free CO2 increased significantly (P<0.05) with increasing temperature, while the pH of the water was almost unchanged throughout the study period. The present study indicated the impact of increased temperature on pesticide toxicity in the aquatic ecosystem.Progressive Agriculture 29 (1): 64-70, 2018 

scholarly journals Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Sidra Perveen ◽  
Shalu Kumari ◽  
Himali Raj ◽  
Shahla Yasmin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drosophila Melanogaster ◽  
Sodium Fluoride ◽  
Ocimum Sanctum ◽  
Activity Assay ◽  
Lipid Peroxidation Assay ◽  
Climbing Ability ◽  
The Impact ◽  
Sublethal Concentrations

Abstract Background Fluoride may induce oxidative stress and apoptosis. It may also lead to neurobehavioural defects including neuromuscular damage. The present study aimed to explore the effects of sub lethal concentrations of sodium fluoride (NaF) on the lifespan and climbing ability of Drosophila melanogaster. In total, 0.6 mg/L and 0.8 mg/L of NaF were selected as sublethal concentrations of NaF for the study. Lifespan was measured and climbing activity assay was performed. Results The study showed significant decrease in lifespan of flies treated with fluoride. With increasing age, significant reduction in climbing activity was observed in flies treated with sodium fluoride as compared to normal (control) flies. Flies treated with tulsi (Ocimum sanctum) and NaF showed increase in lifespan and climbing activity as compared to those treated with NaF only. Lipid peroxidation assay showed significant increase in malondialdehyde (MDA) values in the flies treated with NaF as compared to control. The MDA values decreased significantly in flies treated with tulsi mixed with NaF. Conclusions The results indicate that exposure to sub lethal concentration of NaF may cause oxidative stress and affect the lifespan and climbing activity of D. melanogaster. Tulsi extract may help in reducing the impact of oxidative stress and toxicity caused by NaF.

scholarly journals Changes in the catch composition of artisanal fisheries attributable to dolphin depredation in a Mediterranean marine reserve

2009 ◽  
Vol 66 (4) ◽  
pp. 699-707 ◽  
Author(s):  
Delphine Rocklin ◽  
Marie-Catherine Santoni ◽  
Jean-Michel Culioli ◽  
Jean-Antoine Tomasini ◽  
Dominique Pelletier ◽  
...  
Keyword(s):  
Marine Reserve ◽  
Bottlenose Dolphins ◽  
Artisanal Fisheries ◽  
Catch Per Unit Effort ◽  
Natural Reserve ◽  
Catch Composition ◽  
The Impact ◽  
High Fish ◽  
Fishing Area ◽  
Fish Avoidance

AbstractRocklin, D., Santoni, M-C., Culioli, J-M., Tomasini, J-A., Pelletier, D., and Mouillot, D. 2009. Changes in the catch composition of artisanal fisheries attributable to dolphin depredation in a Mediterranean marine reserve. – ICES Journal of Marine Science, 66: 699–707. There is increasing evidence from previous studies, and from fishers’ observations, that coastal dolphins use fishing nets as an easily accessible feeding source, damaging or depredating fish caught in the nets. This study investigates the impact of dolphin depredation on artisanal trammelnets by analysing the catch composition of 614 artisanal fishing operations in the Bonifacio Strait Natural Reserve (France). Common bottlenose dolphins (Tursiops truncatus) attacked, on average, 12.4% of the nets and damaged 8.3% of the catch. However, attacked nets were characterized by statistically significantly higher catch per unit effort than unattacked ones. Catch composition also differed significantly after dolphin attacks; bentho-pelagic fish were more represented and reef-associated fish less represented. Our results suggest that (i) dolphins are attracted by high fish densities in the fishing area and/or nets, and (ii) their attacks induce specific fish-avoidance behaviour, according to the fish position in the water column. Although dolphins depredate a small part of the catch, damage to nets, not yet assessed in this area, could weaken the benefits that reserves can provide to artisanal fisheries.

THE EFFECT OF SOLID AND POROUS CHANNEL WALLS ON STEADY SWIMMING OF STEELHEAD TROUT ONCORHYNCHUS MYKISS

1993 ◽  
Vol 178 (1) ◽  
pp. 97-108 ◽  
Author(s):  
P. W. Webb
Keyword(s):  
Swimming Speed ◽  
Swimming Performance ◽  
Beat Frequency ◽  
Regression Coefficients ◽  
Steelhead Trout ◽  
Wall Effects ◽  
Fish Swimming ◽  
Wide Channel ◽  
Solid Walls ◽  
Tail Beat

Kinematics and steady swimming performance were recorded for steelhead trout (approximately 12.2 cm in total length) swimming in channels 4.5, 3 and 1.6 cm wide in the centre of a flume 15 cm wide. Channel walls were solid or porous. Tail-beat depth and the length of the propulsive wave were not affected by spacing of either solid or porous walls. The product of tail-beat frequency, F, and amplitude, H, was related to swimming speed, u, and to harmonic mean distance of the tail from the wall, z. For solid walls: FH = 1.01(+/−0.31)u0.67(+/−0.09)z(0.12+/−0.02) and for grid walls: FH = 0.873(+/−0.302)u0.74(+/−0.08)z0.064(+/−0.024), where +/−2 s.e. are shown for regression coefficients. Thus, rates of working were smaller for fish swimming between solid walls, but the reduction due to wall effects decreased with increasing swimming speed. Porous grid walls had less effect on kinematics, except at low swimming speeds. Spacing of solid walls did not affect maximum tail-beat frequency, but maximum tail-beat amplitude decreased with smaller wall widths. Maximum tail-beat amplitude similarly decreased with spacing between grid walls, but maximum tail-beat frequency increased. Walls also reduced maximum swimming speed. Wall effects have not been adequately taken into account in most studies of fish swimming in flumes and fish wheels.

The Kinematics of Swimming in Larvae of the Clawed Frog, Xenopus Laevis

1986 ◽  
Vol 122 (1) ◽  
pp. 1-12 ◽  
Author(s):  
KARIN VON SECKENDORFF HOFF ◽  
RICHARD JOEL WASSERSUG
Keyword(s):  
Xenopus Laevis ◽  
Swimming Speed ◽  
High Speed ◽  
Total Mass ◽  
Beat Frequency ◽  
Maximum Amplitude ◽  
Open Water ◽  
Computer Assisted ◽  
Anuran Larvae ◽  
Tail Beat

The kinematics of swimming in larval Xenopus laevis has been studied using computer-assisted analysis of high-speed (200 frames s−1) ciné records. The major findings are as follows. 1. At speeds below 6 body lengths (L) per second, tail beat frequency is approximately 10 Hz and, unlike for most aquatic vertebrates, is not correlated with specific swimming speed. At higher speeds, tail beat frequency and speed are positively correlated. 2. Xenopus tadpoles show an increase in the maximum amplitude of the tail beat with increasing velocity up to approximately 6Ls−1. Above that speed amplitude approaches an asymptote at 20 % of body length. 3. Anterior yaw is absent at velocities below 6Ls−1, unlike for other anuran larvae, but is present at higher speeds. 4. At speeds below 6Ls−1 there is a positive linear relationship between length of the propulsive wave (λ) and specific swimming speed. At higher speeds wavelength is constant at approximately 0.8L. 5. There is a shift in the modulation of wavelength and tail beat frequency with swimming speed around 5.6Ls−1, suggesting two different swimming modes. The slower mode is used during open water cruising and suspension feeding. The faster, sprinting mode may be used to avoid predators. 6. Froude efficiencies are similar to those reported for fishes and other anuran larvae. 7. Unlike Rana and Bufo larvae, the axial muscle mass of Xenopus increases dramatically with size from less than 10% of total mass for the smallest animals to more than 45% of total mass for the largest animals. This increase is consistent with maintaining high locomotor performance throughout development.

scholarly journals Evaluation on Toxicity Level of Terminalia catappa Leaves Extract on Selected Cyprinids under Different Bath Concentrations

2020 ◽  
Vol 43 (4) ◽  
Author(s):  
Emi Fazlina Hashim ◽  
Irence John ◽  
Intan Faraha A Ghani ◽  
Mohammad Noor Amal Azmai
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Safety Margin ◽  
Probit Analysis ◽  
Time Interval ◽  
Acute Toxicity Test ◽  
High Concentration ◽  
Terminalia Catappa ◽  
Cyprinid Species ◽  
Goldfish Carassius Auratus

This study aimed to determine the lethal concentration (LC50) of Terminalia catappa leaves extract on three cyprinid species; carp (Cyprinus carpio), goldfish (Carassius auratus) and tiger barb (Puntigrus tetrazona) through the acute toxicity test. The leaves of T. catappa were extracted with methanol and prepared in various immersion concentrations (40, 80, 100, 150, 200, 250, 300, and 350 mg/L). These extracts were immersed in the aquarium and left for 24 h before performing the acute toxicity test. The water quality was also analyzed before and after adding the extract immersions into the aquarium. The acute toxicity test conducted for 96 h with 10 fishes of each cyprinid species (4.0-6.0 cm length) in 30 L water capacity aquarium. The mortality of each cyprinid species was recorded at 24 h time interval and LC50 of the extracts throughout 96 hours was determined through the probit analysis application. Specifically, the LC50 of T. catappa leaves extract were 349.89, 338.65 and 318.48 mg/L exhibited for carp, goldfish and tiger barb, respectively. A high concentration range of any plant-based extract has the potential to become toxic to particular fishes. Thus, it is an effort from this study to identify the safety margin of T. catappa leaves extract before its therapeutic values can be further manipulated and elucidated in aquaculture research.

scholarly journals Acute toxicity of nanosized beet pectin

2015 ◽  
Vol 96 (2) ◽  
pp. 208-213
Author(s):  
N Yu Alimzhanov ◽  
I Sh Chakeev ◽  
Sh Zh Zhorobekova ◽  
I O Kudaybergenova ◽  
B N Lepshin
Keyword(s):  
Acute Toxicity ◽  
Structural Changes ◽  
Least Squares Method ◽  
Histological Study ◽  
Lethal Dose ◽  
Study Drug ◽  
Biological Properties ◽  
Probit Analysis ◽  
Pectin Substance ◽  
Hazard Class

Aim. To determine the acute toxicity and hazard class of nanosized low-esterified beet pectin.Methods. To study the acute toxicity of substances, Kerber’s method was used. Probit analysis for different values of lethal dose calculated by least squares method, as well as morphologic studies, statistical analysis (non-parametric methods - Wilcoxon-Mann-Whitney test) were used. Pectin toxicity was studies on 40 mature Wistar rats of both gender and body weight of 160-230 g.Results. Enteral administration of 12 000 mg/kg of pectin did not affect the general condition and did not lead to lethal outcome. The following values of lethal doses were calculated using probit analysis: LD16=34 990.6542056074≈35 g/kg, LD50=74 242.9906542057≈74 g/kg, LD84=113 495.327102804≈113 g/kg, LD100=133 121.495327103≈133 g/kg. Histological study of rat organ tissues that received 12 000 mg/kg of pectin showed no structural changes in tissues of examined organs. Study drug - nanosized low molecular weight pectin, might be referred to hazard class IV (low hazard substances) according to GOST 12.1.007-76. and classification K.K. Sidorov Pectin substance may be considered as practically nontoxic drug (LD50 >10,000 mg/kg), which corresponds to Class V compounds according to Hodge and Sterner classification and classification by K.K. Sidorov.Conclusion. The results indicate complete safety of nanosized forms of pectin, which opens up prospects for further studies of the biological properties of this substance.

scholarly journals Uji Toksisitas Akut Ekstrak Etanol Daun Sapu Jagad (Isotoma longiflora (L) Presl.) pada Mencit Galur Mus muculus

2017 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Ira Safitri ◽  
Inayah Inayah
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Herbal Medicine ◽  
Acute Toxicity ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Probit Analysis ◽  
Acute Toxicity Test ◽  
Pharmacological Effects ◽  
Per Oral

Sapu Jagad (Isotoma longiflora (L) Presl.) plant has been empirically used as traditional medicine. Some studies showthat this plant has pharmacological effects as antibiotic, anticancer, and analgetic. It is of importance to conduct studyin finding out the safetiness of this plant as herbal medicine. Therefore, we conducted study to find out lethal dose ofits leaves on mice (Mus muculus) using acute toxicity test. Several doses have been given to certain groups to find outits effect including death. The extract has been given one time per oral. Then, we recorded the clinical signs and deathof mice until 14 days. The data was analyzed using probit analysis to measure LD50. This study shows that ethanolextract of Sapu Jagad leaves has LD50 12.610 mg/kgBW and toxicity of central nervous system proven by seizureending with death. As conclusion, this extract has toxicity especially to central nervous system.

scholarly journals Effect of exposure to sublethal concentrations of zinc cyanide on tissue ATPase activity in the fresh water fish, Cirrhinus mrigala (Ham)

2012 ◽  
Vol 64 (1) ◽  
pp. 257-263 ◽  
Author(s):  
Shwetha Dube ◽  
B.B. Hosetti
Keyword(s):  
Adaptive Response ◽  
Aquatic Organisms ◽  
Fresh Water Fish ◽  
Cirrhinus Mrigala ◽  
Biomarkers Of Exposure ◽  
Maximum Inhibition ◽  
Water Fish ◽  
Treated Fish ◽  
Cyanide Intoxication ◽  
Sublethal Concentrations

The present study was conducted to evaluate the effects of zinc cyanide (Zn(CN)2) on the ATPase (Na+-K+-, Mg2+, Ca2+) activity of the freshwater fish Cirrhinus mrigala (Ham). Fish were exposed to two sublethal concentrations (0.114 and 0.068 mg/L) and the effects were studied in the liver, muscle and gill tissues at the end of 5, 10 and 15 days of exposure. Cyanide intoxication resulted in marked changes in ATPases, represented by a significant decrease in the activities. Maximum inhibition was observed at higher concentration. The order of inhibition in the activity was Na+-K+-> Ca2+ > Mg2+ ATPase. These results imply an immediate adaptive response to the stress, demonstrating alterations in ATPase activities in the tissues of cyanide-treated fish. The present study clearly proposes that ATPases can be used as biomarkers of exposure of aquatic organisms to cyanide intoxication.

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