Acid formation in gastroenterological patients with colonization of the stomach with virulent and non-virulent strains of helicobacter

Objective. To prove that increased acid production in patients with gastric ulcer and duodenal ulcer is associated with the impact of the virulent strains of Helicobacter pylori but due not to the persistence of non-virulent strains.Materials and methods. Patients with active gastroduodenal ulcer and patients with active chronic pancreatitis accompanied by the gastritis were compared in the respect of the level of pH in the antrum and corpus gastricum, as well as Helicobacter pylori virulence according to the presence cagA gene, especially in combination with vacA allele s1 / m1 (if any of the strains were found in gastric biopsy specimen).Results. In patients with gastric ulcer the average values of pH were significantly lower, both in the antrum and corpus gastricum, than in patients with chronic pancreatitis accompanied by gastritis. Helicobacter pylori strains were found only in half of the patients, either in the gastric ulcer group or in the group of chronic pancreatitis accompanied by the gastritis. Significant difference was revealed after virulent genes identification: virulent strains prevailed in patients with gastric ulcer and in contrast to the prevalence of non-virulent strains in patients with chronic pancreatitis accompanied by the gastritis (Mann-Whitney test, p = 0.001). Since there is no available data that Helicobacter has an affinity for a highly acidic medium in comparison with moderately acidic medium, it is concluded that just primary colonization of the stomach with virulent strains results in hyperacidity (as the consequence of cytotoxicity) and that persistence of non-virulent strains hardly effects hyperacidity.

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A Retrospective Experience of Helicobacter pylori Histology in a Large Sample of Subjects in Northern Italy

Life ◽

10.3390/life11070650 ◽

2021 ◽

Vol 11 (7) ◽

pp. 650

Author(s):

Davide Giuseppe Ribaldone ◽

Carlo Zurlo ◽

Sharmila Fagoonee ◽

Chiara Rosso ◽

Angelo Armandi ◽

...

Keyword(s):

Helicobacter Pylori ◽

Large Population ◽

Population Data ◽

Signet Ring Cell ◽

Current Data ◽

Low Grade ◽

Gastric Cardia Adenocarcinoma ◽

Significant Difference ◽

H Pylori ◽

The Impact

Updated data about the prevalence of Helicobacter pylori (H. pylori) and its correlation with histological results are scarce. The aim of our study was to provide current data on the impact of H. pylori in a third-level endoscopy service. We performed a large, retrospective study analyzing the results of all histological samples of gastroscopy from the year 2019. In total, 1512 subjects were included. The prevalence of H. pylori was 16.8%. A significant difference between the prevalence in subjects born in Italy and those from eastern Europe, south America, or Africa was found (p < 0.0001, p = 0.006, and p = 0.0006, respectively). An association was found between H. pylori and active superficial gastritis (p < 0.0001). Current H. pylori and/or a previous finding of H. pylori was related to antral atrophy (p < 0.0001). Fifteen patients had low-grade dysplasia. There were no statistically significant associations with current or past H. pylori infection. One patient presented gastric cardia adenocarcinoma with regular gastric mucosa. One patient, H. pylori positive, was diagnosed with gastric signet ring cell adenocarcinoma in a setting of diffuse atrophy, without metaplasia.. Our study provides updated, solid (biopsy diagnosis and large population) data on the prevalence of H. pylori infection in a representative region of southern Europe.

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The impact of Helicobacter pylori on the presence of Barrett's esophagus in Azerbaijan, a high-prevalence area of infection

Diseases of the Esophagus ◽

10.1093/dote/doz053 ◽

2019 ◽

Vol 32 (11) ◽

Cited By ~ 2

Author(s):

S Aghayeva ◽

K C Mara ◽

D A Katzka

Keyword(s):

Helicobacter Pylori ◽

Intestinal Metaplasia ◽

Barrett’S Esophagus ◽

Barrett's Esophagus ◽

Erosive Esophagitis ◽

Control Group ◽

Significant Difference ◽

High Prevalence ◽

H Pylori ◽

The Impact

SUMMARY There is a strong evidence that Helicobacter pylori infection is inversely associated with Barrett's esophagus. In a high-prevalence region of H. pylori, low rates of esophageal cancer and its precursor BE may indicate its preventive effect. The aim of this study is to determine the impact of H. pylori on characteristics of Barrett's esophagus. A total of 3317 outpatient upper endoscopy reports from 2013 to 2015 from an urban center in Azerbaijan from all patients with dyspepsia were retrospectively analyzed for patients with Barrett's esophagus. This was matched in a 1:2 ratio to age and gender matched control patients without Barrett's esophagus. The prevalence of H. pylori on Barrett's esophagus and the randomly selected control group were compared. There were 83 patients with BE and 167 control group cases. Biopsy-proven BE was diagnosed in 83 patients: 39 (47%) females, with mean age 43.1 ± 13.3 years. Of these, 13 (15.7%) had long segment and 70 (84.3%) had short segment Barrett's esophagus. A control group included 167 patients: 78 (46.7%) females, with mean age (45.8 ± 13.9). All patients were Caucasians. The rates of gastric inflammation, the presence of atrophy, and intestinal metaplasia in gastric specimens did not differ in patients versus controls. The prevalence of H. pylori was determined as 63.2% in male and 61.5 in female groups (odd ratio (OR) = 0.99 95%CI 0.97, 1.01; P = 0.22). Inflammation of gastric mucosa was strongly associated with the infection (67% vs. 33%; OR = 4.46 95% CI: 2.01, 9.92, P < 0.001). Atrophy was noted in majority of H. pylori-positive cases (OR = 1.43, 95% CI: 0.36, 5.65; P = 0.61). Gastric intestinal metaplasia was observed in 55.6% of H. pylori-positive patients and in 44.4% of negative individuals (OR = 0.74, 95% CI: 0.28, 1.94; P = 0.54). There was not a significant difference in the prevalence of HP in BE and control groups; 63.9% were positive for infection in BE cases and 61.7% of controls (OR = 1.10, 95% CI: 0.64, 1.90; P = 0.74). We found that neither presence of erosive esophagitis, length of BE nor dysplasia (45.5% of H. pylori-positive group, whereas 54.5%) was associated with the presence of the H. pylori infection (Table 1). In a predominantly Caucasian nation with a high prevalence of H. pylori gastritis, the presence of H. pylori was not inversely associated with the presence of Barrett's esophagus. These data challenge the mechanistic implications of this association.

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Helicobacter pylori cagA gene Polymorphism in Patients with Gastroduodenal Diseases

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v43i1.35152 ◽

2018 ◽

Vol 43 (1) ◽

pp. 37-43

Author(s):

Mahbuba Chowdhury ◽

Sharmeen Ahmed ◽

A.F.M.A.L Masum Khan ◽

Shirin Tarafdar ◽

Ruhul Amin Miah

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Gastric Carcinoma ◽

Peptide Sequence ◽

Rapid Urease Test ◽

Caga Gene ◽

Urease Test ◽

H Pylori ◽

Epiya Motifs

Helicobacter pylori is a genetically diverse bacterial pathogen and its CagA gene is a major virulence factor that plays an important role in gastroduodenal pathologies. The biological function of cagA depends on tyrosine phosphorylation within the EPIYA (Glutamate-Proline-Isoleucine-Tyrosine-Alanine) motifs at the C-terminal region of the protein. This region may undergo polymorphism to give different types of EPIYA motifs. EPIYA motif diversity may provide a useful tool for prediction of H. pylori pathogenic activity and accurate determination of number and type of cagA EPIYA motifs could identify the virulent H. pylori. The aim of this study was to detect H. pylori cagA gene and its polymorphism in endoscopic gastroduodenal biopsy specimen from patients with gastroduodenal diseases in Bangladesh. This cross sectional study was carried out in the Department of Microbiology & Immunology, Bangabandhu Sheikh Mujib Medical University and Center for Advanced Research in Sciences, University of Dhaka during the period from March 2014 to February 2015. Gastric biopsies were collected from 78 patients with gastritis, duodenal ulcer, gastric ulcer and gastric carcinoma. H. pylori was identified by rapid urease test and ureC gene PCR. Presence of cagA gene and number and pattern of cagA EPIYA motif were determined by PCR. DNA sequencing was carried out to confirm the PCR detection method of cagA EPIYA motif and to analyse their peptide sequence. Among 31(39.7%) H. pylori positive cases, 19 (61.3%) were cagA gene positive in 11(55%) gastritis, 4(66.7%) duodenal ulcer, 2(66.7%) gastric ulcer and 2(100%) gastric carcinoma. A significant association was found between cagA gene and duodenal ulcer (p=˂0.05). EPIYA motif of all H. pylori cagA positive cases showed Western type cagA EPIYA ABC. No East Asian EPIYA ABD motif was found. Majority of gastroduodenal cases (57.9%) had 3 copies of EPIYA (ABC type), 26.3% had 4 copies (ABCC type) while remaining 10.5% had AC and 5.2% AB type EPIYA motif. EPIYA ABC was found in 75% of duodenal ulcer followed by 54.5% of gastritis and 50% of both gastric ulcer and gastric carcinoma patients. EPIYA ABCC motif was found in 50% of gastric ulcer and gastric carcinoma patients. Most of the EPIYA motif was EPIYA ABC and some were ABCC which has the risk of developing gastric carcinoma.

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The Impact Factor of Colon Polyp

10.21203/rs.3.rs-659158/v1 ◽

2021 ◽

Author(s):

Zheng Zhou ◽

Guangming Wang

Keyword(s):

Helicobacter Pylori ◽

Impact Factor ◽

Recurrence Rate ◽

Infection Rate ◽

Colonic Polyp ◽

Helicobacter Pylori Infection ◽

Control Group ◽

Significant Difference ◽

The Impact ◽

The Relationship

Abstract Objective: To evaluate the relationship between Helicobacter pylori and colonic polyp.Methods: The clinical data of 850 patients who underwent both colonoscopy and Helicobacter pylori check were collected in our hospital from January 2018 year to January 2019 year. Patients were divided into polyp group and control group. To analyze the helicobacter pylori infection of two groups, and the relationship among helicobacter pylori infection, eradication treatment and recurrence of colonic polyp. Further to analyze the impact factor of the recurrence of colonic polyp.Results: There were no significant difference in sex and age aspects of two groups. Through SPSS software analysis, the infection rate of helicobater pylori was higher in polyp group than in control group. The recurrence rate of helicobacter pylori positive patients were more higher than helicobacter pylori negative patients. The recurrence rate of helicobacter pylori eradication group was lower than helicobacter pylori no eradication group. the impact factor of the recurrence of colonic polyp include helicobacter pylori infection, pathological type of polyp and the number of polyps.Conclusion: The helicobacter pylori infection rate of colonic polyp group were more higher. The impact factor of colonic polyp recurrence include helicobacter pylori infection, pathological types of polyp and polyp number.

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The Impact of Gastric Juice pH on the Intraluminal Therapy for Helicobacter pylori Infection

Journal of Clinical Medicine ◽

10.3390/jcm9061852 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1852

Author(s):

Yu-Chio Wang ◽

Yen-Po Chen ◽

Cheng-Yu Ho ◽

Ting-Wen Liu ◽

Cheng-Hsin Chu ◽

...

Keyword(s):

Helicobacter Pylori ◽

Success Rate ◽

Gastric Juice ◽

Antimicrobial Agents ◽

Endoscopic Examination ◽

Significant Difference ◽

H Pylori ◽

Upper Abdominal ◽

Normal Acidity ◽

The Impact

Background: Helicobacter pylori (H. pylori) can be topically eradicated in stomach lumen on endoscopic examination. The procedures of intraluminal therapy for H. pylori infection (ILTHPI) include the control of intragastric pH, mucolytic irrigation of the gastric mucosal surface, and a single-dose medicament containing antimicrobial agents. Aims: To detect gastric juice pH and evaluate its impact on the success rate of ILTHPI. Methods: We enrolled 324 patients with upper abdominal discomfort for endoscopic examinations. Among them, 13C-urea breath test was positive in 218 patients, where 100 underwent ILTHPI, and negative in 106. All patients had their gastric juice pH detected and set into three ranges, including normal acidity (pH < 4.0), low-level hypoacidity (pH 4.0–5.5), and high-level hypoacidity (pH ≥ 6.0). The impact of gastric juice pH on the success rate of ILTHPI was evaluated. Results: Distribution of pH level showed no significant difference between two groups of H. pylori-infected patients (p = 0.942). The eradication rate of ILTHPI is significantly lower in patients with gastric juice pH below 4 (p < 0.001). Conclusions: Detection of gastric juice pH in ILTHPI is extremely important. Rapid control of stomach pH at or above 4 for patients prior to ILTHPI is strongly recommended. (NCT03124420).

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Association of Helicobacter pylori restriction endonuclease-replacing gene, hrgA with overt gastrointestinal diseases

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032008000300011 ◽

2008 ◽

Vol 45 (3) ◽

pp. 225-229 ◽

Cited By ~ 1

Author(s):

Manoj G ◽

Santosh K. Tiwari ◽

Vishwas Sharma ◽

Mohammed Aejaz Habeeb ◽

Aleem A. Khan ◽

...

Keyword(s):

Helicobacter Pylori ◽

Surrogate Marker ◽

Gastrointestinal Diseases ◽

Histopathological Analysis ◽

Caga Gene ◽

Oligonucleotide Primers ◽

New Genes ◽

Significant Difference ◽

The Status ◽

H Pylori

BACKGROUND and AIM: Helicobacter pylori has been proven to be responsible for causing various gastrointestinal disorders including gastric adenocarcinoma. Several genes of pathogen (the genes of the cag-PAI, vacA, iceA, and babA) either in combination or independently have been reported to significantly increase the risk of ulceration/gastric carcinoma, with the cagA gene having the strongest predictive value. Pursuit to identify new genes which could serve as a marker of overt disease progression, lead to the discovery of hrgA gene. METHODS: Fifty-six indigenous strains of H. pylori from subjects with various gastric disorder were screened to assess the status of hrgA gene along with the cagA gene using simple polymerase chain reaction using specific oligonucleotide primers. Post-amplification, amplicons were subjected for sequencing to identify any strain specific variations in sequences from the H. pylori isolated from different disease manifestations. Histopathological analysis was done to ascertain any significant change in the histological scores of subjects infected with cagA+/hrgA+ and cagA-/hrg+ strains. RESULTS: All the 56 (100%) subjects amplified with the oligonucleotide primers specific to hrgA gene, whereas 81.71% subjects showed the presence of cagA gene. Sequencing of the amplimers showed 99% homology. Histology of the cagA+/hrgA+ and cagA-/hrg+ subjects did not show any significant difference. CONCLUSION: hrgA gene of Helicobacter pylori is not a ideal surrogate marker for identifying individuals with higher risk of developing overt gastro-duodenal diseases such as neoplasia of the stomach.

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Etiological Factors in Urgent Gastroduodenal Ulcer

PRILOZI ◽

10.1515/prilozi-2015-0068 ◽

2015 ◽

Vol 36 (2) ◽

pp. 203-210

Author(s):

Goran Begovic ◽

Redzep Selmani

Keyword(s):

Helicobacter Pylori ◽

Serological Test ◽

Gastroduodenal Ulcer ◽

Significant Risk ◽

Significant Risk Factor ◽

Gastric Ulcers ◽

The One ◽

A Prospective Study ◽

The University ◽

The Impact

Abstract Aim: The goal of the study is to assess the impact of the etiological and risk factors in the cases of urgent gastroduodenal ulcer. Methods: This is a prospective study which included 67 patients selected randomly, all with urgent, life-threatening complications of the gastroduodenal ulcer, treated at the University Clinic for Digestive Surgery and the University Clinic for Gastroenterohepatology. For the purpose of the study the titer of IgG antibodies to Helicobacter pylori in serum is examined, as well as the use of NSAID and aspirin. We also tested the use of nicotine and alcohol. Besides these factors, we took into consideration the sex and the age. Results: The serological test of Helicobacter pylori was positive in 89.6% of the cases. 31.3% used NSAID and 16.4% used aspirin. Large number, 65.7% of the patients were smokers, while 25.4% used alcohol. With regard to the sex, 83.6% were male and 16.4% were female. The most frequent age group was the one from 41 to 60 years with 40.3% and those between 61 and 80 years of age with 35.8%. Conclusion: The presence of Helicobacter pylori with the urgent gastroduodenal ulcer was exceptionally high, in 89.6% of the duodenal and gastric ulcers. NSAID and aspirin are factors that also influence the etiology of this disease. Smoking is a significant risk factor. Alcohol is less present, but still an important factor.

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Mouse model of gastric infection with cytotoxin-expressing strain of Helicobacter pylori in studying of pathogenesis of chronic gastric ulcer

Journal of Gastroenterology and Hepatology ◽

10.1046/j.1440-1746.1998.01729.x ◽

1998 ◽

Vol 13 (11-s4) ◽

pp. S178-S184 ◽

Cited By ~ 1

Author(s):

PETER KONTUREK ◽

TOMASZ BRZOZOWSKI ◽

STANISLAW KONTUREK ◽

ELZBIETA KARCZEWSKA ◽

ROBERT PAJDO ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Ulcer ◽

Mouse Model ◽

Chronic Gastric Ulcer

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Diversity of Helicobacter pylori CagA gene and its clinical significance

Gastroenterology ◽

10.1016/s0016-5085(01)80277-8 ◽

2001 ◽

Vol 120 (5) ◽

pp. A56-A56

Author(s):

T AZUMA ◽

Y ITO ◽

M DOJO

Keyword(s):

Helicobacter Pylori ◽

Clinical Significance ◽

Caga Gene

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Measuring Impact of Stress in Sandwich Generation Caring for Demented Parents

GeroPsych ◽

10.1024/1662-9647/a000114 ◽

2014 ◽

Vol 27 (4) ◽

pp. 171-179 ◽

Cited By ~ 6

Author(s):

Laurence M. Solberg ◽

Lauren B. Solberg ◽

Emily N. Peterson

Keyword(s):

Factor Analysis ◽

Exploratory Factor Analysis ◽

Social Worker ◽

Resource Availability ◽

Well Being ◽

Sandwich Generation ◽

Item Questionnaire ◽

Significant Difference ◽

Adult Caregivers ◽

The Impact

Stress in caregivers may affect the healthcare recipients receive. We examined the impact of stress experienced by 45 adult caregivers of their elderly demented parents. The participants completed a 32-item questionnaire about the impact of experienced stress. The questionnaire also asked about interventions that might help to reduce the impact of stress. After exploratory factor analysis, we reduced the 32-item questionnaire to 13 items. Results indicated that caregivers experienced stress, anxiety, and sadness. Also, emotional, but not financial or professional, well-being was significantly impacted. There was no significant difference between the impact of caregiver stress on members from the sandwich generation and those from the nonsandwich generation. Meeting with a social worker for resource availability was identified most frequently as a potentially helpful intervention for coping with the impact of stress.

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