Показники неспецифічної резистентності однорічок коропа за інвазії ектопаразитами

Вісник Полтавської державної аграрної академії ◽

10.31210/visnyk2013.02.29 ◽

2013 ◽

pp. 104-106

Author(s):

Ю. В. Лобойко

Keyword(s):

Bactericidal Activity ◽

Phagocytic Activity ◽

Lernaea Cyprinacea ◽

Carp Serum

Наведено дані щодо лізоцимної і бактерицидноїактивності сироватки крові та фагоцитарноїактивності нейтрофілів за різної інтенсивностіінвазії ектопаразитами. Встановлено зниженнялізоцимної, бактерицидної та фагоцитарної ак-тивності сироватки крові коропів за ураженняектопаразитами Lernaea cyprinacea та Dactylogyrusvastator, порівняно з клінічно здоровимирибами. За високої інвазії ектопаразитами (>0,26лерней/г м.т. та >0,53 дактилогірусів /г м.т.)спостерігали вірогідне зниження показників неспе-цифічної резистентності однорічок коропа. The data on lyzocymic and bactericidal activity of serum and blood phagocytic activity ofneutrophils by different intensity of infestation by ectoparasites. The decrease lyzocymic, bactericidal andphagocytic activity of carp serum by lesions ectoparasites Lernaea cyprinacea and Dactylogyrus vastatorcompared with clinically healthy fish.

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TO STUDY THE DYNAMICS OF IMMUNITY IN BLACK-AND-WHITE COWS DEPENDING ON AGE

Molochnoe i miasnoe skotovodstvo ◽

10.33943/mms.2020.33.88.011 ◽

2020 ◽

pp. 49-52

Author(s):

М.В. ДОВЫДЕНКОВА

Keyword(s):

Bactericidal Activity ◽

Phagocytic Activity ◽

Physiological State ◽

Age Groups ◽

Statistical Processing ◽

Natural Resistance ◽

Black And White ◽

Feeding Methods ◽

High Degree ◽

The Relationship

Проведена статистическая обработка сформированной базы данных показателей неспецифической резистентности MS «Excel» с целью изучения динамики иммунитета по параметрам бактерицидной, лизоцимной и фагоцитарной активности у крупного рогатого скота черно-пестрой породы разных возрастов. Было установлено, что средние значения показателей лизоцимной и бактерицидной активности у телят в 6 мес были ниже (47,89% и 49,09%, соответственно), чем у животных в 3—4 года (53,75% и 52,81%). Выведено уравнение регрессии, описывающее взаимосвязь между средними показателями фагоцитарной активности и возрастом при высокой степени достоверности (R2=0,90). Изменения лизоцимной и бактерицидной активности в зависимости от возраста были недостоверными (R2=0,58 и R2=0,49, соответственно). Установлена высокая корреляция между бактерицидной и лизоцимной активностью. Отмечена динамика изменения неспецифического иммунитета у коров различных возрастных групп. Создание баз данных и постоянный мониторинг основных показателей естественной резистентности в зависимости от различных факторов (породы, возраста, стадий лактации, кормления, способов содержания), которые влияют на данные показатели, необходимо для оценки физиологического состояния организма животных и их устойчивости к болезням. Statistical processing of the generated MS "Excel" on indicators of non-specific resistance database was carried out with the aim of studying the dynamics of the development of immunity in terms of bactericidal, lysozyme and phagocytic activity in black-and-white cattle of different ages. It was found that the average values of indicators of lysozyme and bactericidal activity in calves at 6 months were lower (47.89% and 49.09%, respectively) than in animals at the age of 3—4 years (53.75% and 52.81%). The equation of regression was derived, it describes the relationship between the average indicators of phagocytic activity and the age with a high degree of reliability (R2=0.90). The changes in lysozyme and bactericidal activity depending on age were dubious (R2=0.58 and R2=0.49, respectively). The high correlation between bactericidal and lysozyme activity was established. It was noticed that the cows of different age groups experienced the dynamics of changes in nonspecific immunity. The creation of databases and constant monitoring of the main indicators of natural resistance, depending on various factors (breed, age, stages of lactation, feeding, methods of keeping) that affect these indicators, is necessary for assessing the physiological state of the animals’ body of and their resistance to diseases.

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The effect of the drug “Amiksyn” on the immune status of carp

Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies ◽

10.32718/nvlvet-a9525 ◽

2021 ◽

Vol 23 (95) ◽

pp. 167-171

Author(s):

S. I. Kravets ◽

O. V. Krushelnytska

Keyword(s):

B Lymphocytes ◽

Bactericidal Activity ◽

Phagocytic Activity ◽

Fish Farming ◽

Optimal Dose ◽

Negative Effects ◽

Hydrochemical Parameters ◽

Immunological Parameters ◽

Fish Weight ◽

Series Of Experiments

The productivity of pond fish, in particular carp, largely depends on the intensity of use of aquatic bioresources. The aim of the study was to investigate the effect of “Amiksyn” on the immunological parameters of carp fish, namely: the number of T- and B-lymphocytes, immunoglobulins, the level of circulating immune complexes, lysozyme, phagocytic activity and bactericidal activity of serum. 10 fish were selected for hematological and biochemical studies. The research was conducted in the aquarium of the Department of Aquatic Bioresources of Stepan Gzhytskyi NUVMB Lviv. Fish were kept in the pool for 21 days to adapt to the new conditions, and at the beginning of the series of experiments were placed in aquariums with a volume of 200 liters. Aeration and mechanical filtration of water were provided in the tanks. The temperature during the experiments fluctuated slightly and was 18 ± 1.5 °C. The main hydrochemical parameters corresponded to fish farming standards. In order to adjust the immune response, and thus prevent possible manifestations of fish damage by pathogens, we studied the possibility of using the immunostimulant “Amiksyn”, which was used for 5 – 10 – 15 – 20 days and then dosing 5 – 10 – 15 mg/kg body weight Pisces. The use of “Amiksyn” at a dose of 10 mg/kg of fish weight four times during the fifteen-day period with an interval of 5 days provided a decrease in the number of T-lymphocytes by 9.1 % and B-lymphocytes by 23.0 %; caused the activation of non-specific resistance indicators, namely lysozyme activity increased by 28.7 %, phagocytic activity of leukocytes increased by 14.0 % and bactericidal activity of serum increased by 19.0 %. Therefore, to adjust the immune system and stimulate the metabolic processes of fish in order to prevent the negative effects of various pathogens, the most optimal dose was 10 mg/kg of fish weight within 15 days of use.

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THE EFFECT OF PREBIOTIC HEMIV ON THE NATURAL RESISTANCE AND QUALITY OF MEAT OF BROILER CHICKENS

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.202103014 ◽

2021 ◽

Vol 1 (3) ◽

pp. 329-334

Author(s):

A.A. Reznichenko ◽

◽

V.I. Dorozhkin ◽

Keyword(s):

Body Weight ◽

Blood Serum ◽

Meat Quality ◽

Bactericidal Activity ◽

Phagocytic Activity ◽

Broiler Chickens ◽

Natural Resistance

We studied the effect of prebiotic hemiv on natural resistance and meat quality of broiler chickens. As a result of the conducted studies, it was found that after using the drug at a dose of 0,6 g/kg of body weight, there was an increase in the phagocytic activity of pseudoeosinophils and the bactericidal activity of blood serum by 34,3 and 22,4%, respectively, compared with the control. After the use of hemiv at a dose of 0,9 g/kg, there was also a statistically confirmed increase in the bactericidal activity of blood serum and phagocytic activity of pseudoeosinophils by 20,6 and 29,1%. At studying the effect of the drug on the quality of meat, it was found that it belongs to the mature and benign and can be eaten without restrictions.

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COMPLEX EVALUATION OF RESISTANCE AND STRESS STEADINESS OF CALVES

Animal Breeding and Genetics ◽

10.31073/abg.51.13 ◽

2018 ◽

Vol 51 ◽

pp. 101-106

Author(s):

N. N. Makovska ◽

O. D. Biryukova ◽

K. V. Bodriashova

Keyword(s):

Analysis Of Variance ◽

Bactericidal Activity ◽

Phagocytic Activity ◽

Live Weight ◽

Natural Resistance ◽

Farm Animals ◽

Positive Correlation ◽

Skin Fold ◽

Complex Evaluation

The aim of the work was to conduct the complex evaluation of the state of heterospecific resistance and stress steadiness of organism of calves. Materials and methods of researches. A complex scheme was approved for determination of heterospecific resistance of farm animals, which includes application of haematological and immunobiological researches. The work was carried out in "Khrystynivske" breeding farmat the calves of Ukrainian Red-and-White Dairy breed (n = 20) at the age of 2 months. The father of all the investigated calves was bull Inhibitor 402151. Heterospecific resistance of calves was determined by generally accepted methods. The amount of leucocytes and erythrocytes of blood, phagocytic activity of leucocytes and phagocytosis intensity, lysozyme and bactericidal activity of serum were determined. The adapted eosinophilic test on stress steadiness and histamine test were applied for determination of general immunoreactiveness as a screening test for farm animals. The biometrical processing of data was carried out using М. A. Plokhinskiy method and software Microsoft Excel and Statistica6.0. Results of researches. Phagocytic activity of blood leucocytes, on average, was about 56%, and phagocytosis intensity– 3,5±0,56 microbial cells absorbed by an active leucocyte. The average relative bulge of skin fold (Rv) was about 52% at carrying out histamine test. It is stated that 57% of the investigated animals had a normal immune response, about 24% – mionectic, and 2 heifers were in immunosuppression state. There were 3 individuals with high immunoreactiveness (Rv≥100%). Indexes of bactericidal activity of serum at the investigated calves were from 2,6% to 82%, and on average it was 33,7%±4,9 (р<0,001), and activity of lysozyme was from 1,4% to 83,1% and 21,6%±4,8 (р<0,001) on average. It is noted, that animals which had lower live weight at birth were characterized by lower level of bactericidal activity of serum. Rectilinear correlation dependence between the indexes of phagocytic activity of leucocytes and bactericidal activity of serum (r=+0,54±0,16; р<0,001) was revealed. Positive correlation between bactericidal and lysozyme activity of serum (r=+0, 61±0,15; р<0,001) was found. Weak, but significant correlation between the relative bulge of skin fold (Rv) and phagocytic activity of leucocytes indicates that a histamine test is the adequate marker of heterospecific resistance. The animals which had the greatest live weight at birth were characterized by the greatest level of phagocytic activity of leucocytes of blood, and positive correlation between live weight of new-born calves and indexes of phagocytic activity of leucocytes of blood (r = +0,28 ± 0,13 р<0,01) indicates it. An inverse correlation was also revealed between live weight at the age of 2 months and relative bulge of skin fold (r=-0, 18±0,11; р<0,05), which specifies in the presence of growth delay of calves which are characterized by mionectic reactivity in relation to a heterospecific irritant (histamine). The data of eosinophilic test showed that stress-sensitive individuals were about 58 %. The analysis of variance showed reliable influence of a stress status on live weight at the age of 2 months (Р<0,05) and influence of immunoreactiveness on live weight at the age of 6 months (Р<0,05). It should be noted in addition, that impact of a stress status on live weight at the age of 12 months approached to reliable value (Р=0,06). In general, there was declining of force of influence of both factors on live weight with age. Reliable dependence of a stress status and immunoreactiveness on age of first insemination and age of first calving wasn’t revealed. Conclusions. Individual variability on the indexes of natural resistance and high level of heterospecific resistance in the most of the investigated calves were revealed in progeny of bull Inhibitor. The analysis of variance revealed direct influence of heterospecific resistance on the indexes of individual development of animals. There are pre-conditions in relation to screening application of histamine test and eosinophilic test as informing and economic accessible ones for determination of level of heterospecific resistance and stress steadiness in the system of complex estimation of specifics of pedigree resources.

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Phagocytic and bactericidal activity of blood thrombocytes in carps (Cyprinus carpio)

Veterinární Medicína ◽

10.17221/5798-vetmed ◽

2012 ◽

Vol 47 (No. 1) ◽

pp. 21-25 ◽

Cited By ~ 11

Author(s):

M. STOSIK ◽

W. DEPTUŁA ◽

M. TRÁVNIČEK ◽

K. BALDY-CHUDZIK

Keyword(s):

Staphylococcus Aureus ◽

Cyprinus Carpio ◽

Bactericidal Activity ◽

Phagocytic Activity ◽

Ontogenetic Development ◽

Phagocytic Index ◽

Intracellular Killing

The studies aimed at monitoring development of phagocytic and bactericidal activity in carps in the course of their ontogenetic development. The studies were performed using the techniques described by Mantur et al. (1986a, b), adapted to fish. Results were expressed in thrombocyte phagocytic index (Ipt), percentage of phagocyting thrombocytes (%tp), percentage of ingested bacteria (%bp) and in the index of intracellular killing by thrombocytes (Ibt). Number of thrombocytes was also examined using the technique of Dessi. Results of testing thrombocyte capacity to ingest the standard Staphylococcus aureus 209P strain showed that Ipt and %tp increased gradually in carps between the age of 3 and 17 months. In older carps, 19 to 29 months of age, as well as in spawners the growth in activity paralelled the ontogenetic development within Ipt values while %tp values remained at a similar level. Phagocytic activity of thrombocytes expressed in Ipt was reflected also by %bp values, which was particularly evident in fish aging 23 months to 5 years. In the case of Ibt, values of the index were not related in any way to stage of ontogeny or sex of the fish. Number of thrombocytes in carps aged 3 months to 5 years was increasing in parallel to their ontogenetic development.

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Nonspecific reactivity of the rabbits organism when exposed to cysticercosis

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9424 ◽

2019 ◽

Vol 21 (94) ◽

pp. 132-135

Author(s):

Y. V. Duda

Keyword(s):

Blood Serum ◽

Bactericidal Activity ◽

Immune Complexes ◽

Research Group ◽

Phagocytic Activity ◽

Lysozyme Activity ◽

Circulating Immune Complexes ◽

Control Group ◽

E Coli ◽

Test Culture

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of individual invasive diseases, especially cysticercosis, which is caused by the larval Cysticercus pisiformis stage of the mature cestode Taenia pisiformis. The study was conducted on 38 male rabbits age 3–4 months, weighing 3.5–4.0 kg of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). After blood collection, slaughter was conducted and the presence of cysticercus cysts was visually determined in the organisms of the rabbits. Control animals received a balanced standard granular feed and water without limit; research group in addition to the standard granulated feed with water received flaccid hay. The animals were kept in mesh single-tier cages in the room, according to the current veterinary and sanitary standards. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2500000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by the method Smirnova A.V. and Kuzmina T.A. in relation to the E. coli microbial test-culture 055K59№3912/41. The serum lysozyme activity was determined by the Nephelometric method using the Dorofachuk V.G. method to the microbial test culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined using polyethylene glycol in borate buffer (pH 8.4). It was found that in blood of sick animals phagocytic activity is lower than in blood of healthy ones (respectively, 41.82 ± 1.51% versus 47.38 ± 1.10%, P < 0.01). The main cells involved in phagocytosis are leukocytes, in particular neutrophils and eosinophils. A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from cysticercosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 0.51 units (P < 0.05) as compared to the control group and correlated with the index of phagocytic activity. An important element of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 12.88% (P < 0.01) and lysozyme activity in rabbits with Cysticercus pisiformis also indicates a weakening of the factors of nonspecific natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium (9.14 ± 0.40 vs 5.58 ± 0.50) and small (9.21 ± 1.35 vs 4.64 ± 0.68) CIC for cysticercosis, respectively, 1.64 (P < 0.01) and 1.98 times (P < 0.001) against the control. This indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the combination of specific antibodies with the products of the exchange of helminths.

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Use of probiotic lactobacilli to increase non-specific resistance and productivity of rabbits

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-136 ◽

2020 ◽

Author(s):

A.N. Ovcharova ◽

Keyword(s):

Growth Rate ◽

Bactericidal Activity ◽

Phagocytic Activity ◽

Specific Resistance ◽

Live Weight ◽

Phagocytic Index ◽

Control Group ◽

Control Parameters ◽

Probiotic Lactobacilli ◽

Experimental Group

The article presents the results of the effectiveness of probiotic lactobacilli on the productivity and non-specific resistance of rabbits. The phagocytic activity of neutrophils increased by 10 %, which turns out to be statistically significant. The bactericidal activity of blood increased by 8 % in comparison with the control group; the phagocytic index was also higher in the experimental group by 2.02. The content of lysozyme in the blood serum of rabbits in the experimental group was significantly higher than the control parameters by 18.6 micrograms/ml. As tentative results, we report an increase in the live weight and growth rate in the experimental group of rabbits.

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