scholarly journals The effect of the drug “Amiksyn” on the immune status of carp

2021 ◽  
Vol 23 (95) ◽  
pp. 167-171
Author(s):  
S. I. Kravets ◽  
O. V. Krushelnytska
Keyword(s):  
B Lymphocytes ◽  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Fish Farming ◽  
Optimal Dose ◽  
Negative Effects ◽  
Hydrochemical Parameters ◽  
Immunological Parameters ◽  
Fish Weight ◽  
Series Of Experiments

The productivity of pond fish, in particular carp, largely depends on the intensity of use of aquatic bioresources. The aim of the study was to investigate the effect of “Amiksyn” on the immunological parameters of carp fish, namely: the number of T- and B-lymphocytes, immunoglobulins, the level of circulating immune complexes, lysozyme, phagocytic activity and bactericidal activity of serum. 10 fish were selected for hematological and biochemical studies. The research was conducted in the aquarium of the Department of Aquatic Bioresources of Stepan Gzhytskyi NUVMB Lviv. Fish were kept in the pool for 21 days to adapt to the new conditions, and at the beginning of the series of experiments were placed in aquariums with a volume of 200 liters. Aeration and mechanical filtration of water were provided in the tanks. The temperature during the experiments fluctuated slightly and was 18 ± 1.5 °C. The main hydrochemical parameters corresponded to fish farming standards. In order to adjust the immune response, and thus prevent possible manifestations of fish damage by pathogens, we studied the possibility of using the immunostimulant “Amiksyn”, which was used for 5 – 10 – 15 – 20 days and then dosing 5 – 10 – 15 mg/kg body weight Pisces. The use of “Amiksyn” at a dose of 10 mg/kg of fish weight four times during the fifteen-day period with an interval of 5 days provided a decrease in the number of T-lymphocytes by 9.1 % and B-lymphocytes by 23.0 %; caused the activation of non-specific resistance indicators, namely lysozyme activity increased by 28.7 %, phagocytic activity of leukocytes increased by 14.0 % and bactericidal activity of serum increased by 19.0 %. Therefore, to adjust the immune system and stimulate the metabolic processes of fish in order to prevent the negative effects of various pathogens, the most optimal dose was 10 mg/kg of fish weight within 15 days of use.

NEW BIOLOGICAL PREPARATION FOR ALFALFA SILAGE MAKING

1970 ◽  
pp. 36-40
Author(s):  
V. P. Klimenko ◽  
V. М. Kosolapov ◽  
V. G. Kosolapova ◽  
К. Е. Yurtaeva
Keyword(s):  
Optimal Dose ◽  
Energy Value ◽  
New Development ◽  
Basic Parameters ◽  
Excess Moisture ◽  
Series Of Experiments ◽  
Alfalfa Silage ◽  
Biological Additive ◽  
Technical Centre ◽  
Nutrients Digestibility

The data on assessment the preservative action of new enzymatic multisystem in combination with bacterial silage additive Silzak at alfalfa ensiling are presented in this study. It is known that alfalfa is a nonensiling legume grass because of excess moisture, sugar deficit and high content of crude protein. The experiments on alfalfa ensiling were conducted with application the enzymatic multisystem FM-1 under laboratory and research-and-production conditions. The multisystem was developed by the specialists of science-and-technical centre «Lecbiotech» in cooperation with scientists of Williams Fodder Research Institute. The optimal dose of FM-1 application and its preservative effectiveness in comparison with chemical conservant were determined in technological experiments. Obtained silage was evaluated on basic parameters: nutrients and organic acids content, ammonia, active acidity. The influence of the tested biological additive on the nutrients digestibility and energy value of alfalfa silage was estimated in experiments with hog lambs. Total results have shown the efficiency of the new development. Lucerne silage, prepared with application of mixture FM-1 and Silzak, was characterized by the better parameters of nutrients digestibility and energy value than at using a chemical conservant AIV 3 plus. The optimal dose of enzymatic multisystem application, as 90 gram per ton of fresh plant mass, was determined in the series of experiments.

TO STUDY THE DYNAMICS OF IMMUNITY IN BLACK-AND-WHITE COWS DEPENDING ON AGE

2020 ◽  
pp. 49-52
Author(s):  
М.В. ДОВЫДЕНКОВА
Keyword(s):  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Physiological State ◽  
Age Groups ◽  
Statistical Processing ◽  
Natural Resistance ◽  
Black And White ◽  
Feeding Methods ◽  
High Degree ◽  
The Relationship

Проведена статистическая обработка сформированной базы данных показателей неспецифической резистентности MS «Excel» с целью изучения динамики иммунитета по параметрам бактерицидной, лизоцимной и фагоцитарной активности у крупного рогатого скота черно-пестрой породы разных возрастов. Было установлено, что средние значения показателей лизоцимной и бактерицидной активности у телят в 6 мес были ниже (47,89% и 49,09%, соответственно), чем у животных в 3—4 года (53,75% и 52,81%). Выведено уравнение регрессии, описывающее взаимосвязь между средними показателями фагоцитарной активности и возрастом при высокой степени достоверности (R2=0,90). Изменения лизоцимной и бактерицидной активности в зависимости от возраста были недостоверными (R2=0,58 и R2=0,49, соответственно). Установлена высокая корреляция между бактерицидной и лизоцимной активностью. Отмечена динамика изменения неспецифического иммунитета у коров различных возрастных групп. Создание баз данных и постоянный мониторинг основных показателей естественной резистентности в зависимости от различных факторов (породы, возраста, стадий лактации, кормления, способов содержания), которые влияют на данные показатели, необходимо для оценки физиологического состояния организма животных и их устойчивости к болезням. Statistical processing of the generated MS "Excel" on indicators of non-specific resistance database was carried out with the aim of studying the dynamics of the development of immunity in terms of bactericidal, lysozyme and phagocytic activity in black-and-white cattle of different ages. It was found that the average values of indicators of lysozyme and bactericidal activity in calves at 6 months were lower (47.89% and 49.09%, respectively) than in animals at the age of 3—4 years (53.75% and 52.81%). The equation of regression was derived, it describes the relationship between the average indicators of phagocytic activity and the age with a high degree of reliability (R2=0.90). The changes in lysozyme and bactericidal activity depending on age were dubious (R2=0.58 and R2=0.49, respectively). The high correlation between bactericidal and lysozyme activity was established. It was noticed that the cows of different age groups experienced the dynamics of changes in nonspecific immunity. The creation of databases and constant monitoring of the main indicators of natural resistance, depending on various factors (breed, age, stages of lactation, feeding, methods of keeping) that affect these indicators, is necessary for assessing the physiological state of the animals’ body of and their resistance to diseases.

scholarly journals EVIDENCE FOR IDENTITY OR CLOSE ASSOCIATION OF THE Fc RECEPTOR OF B LYMPHOCYTES AND ALLOANTIGENS DETERMINED BY THE Ir REGION OF THE H-2 COMPLEX

1974 ◽  
Vol 140 (3) ◽  
pp. 779-796 ◽  
Author(s):  
Howard B. Dickler ◽  
David H. Sachs
Keyword(s):  
B Lymphocytes ◽  
B Lymphocyte ◽  
Close Association ◽  
Surface Membrane ◽  
Fc Receptor ◽  
F1 Hybrid ◽  
Ia Antigens ◽  
Series Of Experiments ◽  
Mouse Lymphocytes ◽  
Mouse Antibody

Immunoglobulin complexes, composed of heat-aggregated human Ig, were shown to bind to mouse B lymphocytes of a variety of strains, but not to either thymocytes or thymus-derived (T) lymphocytes under a variety of conditions. It was shown that this binding was not due to either natural human antibodies against mouse nor to nonspecific binding of human Ig by mouse lymphocytes. Such complexes were shown to bind to the same sites which bind mouse antibody-antigen complexes. This site is known as the Fc receptor. The binding of Ig complexes to mouse B lymphocytes was markedly inhibited by pretreatment of the lymphocytes with anti-H-2 antisera. A series of experiments indicated the specificity of this result, including the fact that this inhibition was shown not to be due to the artifact of shedding of H-2 antibody-antigen complexes, nor to nonspecific steric inhibition. The antibodies within anti-H-2 antisera which were responsible for this inhibition were specific for alloantigens associated with the Ir region of the H-2 complex (Ia antigens). Antiserum specific for these Ia antigens produced inhibition, whereas antisera specific for antigens determined by the K or D regions of the H-2 complex did not. Evidence was obtained using F1 hybrid cells that at least some Ia antigens of both parental types are expressed on every B lymphocyte (i.e. codominant expression). These data indicate that the Fc receptor and a series of alloantigens controlled by the Ir region of the H-2 complex are identical or closely associated on the B-lymphocyte surface membrane. This observation may have implications for the mechanism of control of the immune response.

scholarly journals An Improved Robust Method for Pose Estimation of Cylindrical Parts with Interference Features

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2234 ◽  
Author(s):  
Jieyu Zhang ◽  
Yuanying Qiu ◽  
Xuechao Duan ◽  
Kangli Xu ◽  
Changqi Yang
Keyword(s):  
Laser Scanning ◽  
Robust Method ◽  
Negative Effects ◽  
Profile Fitting ◽  
Straight Line ◽  
Intelligent Measurement ◽  
Cylindrical Parts ◽  
Point Set ◽  
Aerospace Assembly ◽  
Series Of Experiments

Horizontal docking assembly is a fundamental process in the aerospace assembly, where intelligent measurement and adjustable support systems are urgently needed to achieve higher automation and precision. Thus, a laser scanning approach is employed to obtain the point cloud from a laser scanning sensor. And a method of section profile fitting is put forward to solve the pose parameters from the data cloud acquired by the laser scanning sensor. Firstly, the data is segmented into planar profiles by a series of parallel planes, and ellipse fitting is employed to estimate each center of the section profiles. Secondly, the pose of the part can be obtained through a spatial straight line fitting with these profile centers. However, there may be some interference features on the surface of the parts in the practical assembly process, which will cause negative effects to the measurement. Aiming at the interferences, a robust method improved from M-estimation and RANSAC is proposed to enhance the measurement robustness. The proportion of the inner points in a whole profile point set is set as a judgment criterion to validate each planar profile. Finally, a prototype is fabricated, a series of experiments have been conducted to verify the proposed method.

scholarly journals Evaluation of Standard- and High-Dose Daptomycin versus Linezolid against Vancomycin-Resistant Enterococcus Isolates in anIn VitroPharmacokinetic/Pharmacodynamic Model with Simulated Endocardial Vegetations

2012 ◽  
Vol 56 (6) ◽  
pp. 3174-3180 ◽  
Author(s):  
Ashley D. Hall ◽  
Molly E. Steed ◽  
Cesar A. Arias ◽  
Barbara E. Murray ◽  
Michael J. Rybak
Keyword(s):  
Bactericidal Activity ◽  
Optimal Dose ◽  
Pharmacodynamic Model ◽  
Colony Count ◽  
High Dose ◽  
Vancomycin Resistant Enterococcus ◽  
Content Type ◽  
Sustained Reduction ◽  
Vancomycin Resistant

ABSTRACTDaptomycin MICs for enterococci are typically 1- to 2-fold higher than those forStaphylococcus aureus, and there is an imminent need to establish the optimal dose for appropriate treatment of enterococcal infections. We investigated the bactericidal activity of daptomycin at various dose exposures compared to that of linezolid against vancomycin-resistant enterococcus (VRE) in anin vitropharmacokinetic/pharmacodynamic model utilizing simulated endocardial vegetations over 96 h. Daptomycin at doses of 6, 8, 10, and 12 mg/kg of body weight/day and linezolid at a dose of 600 mg every 12 h were evaluated against two clinical vancomycin-resistantEnterococcus faeciumstrains (EFm11499 and 09-184D1051), one of which was linezolid resistant (09-184D1051), and one clinical vancomycin-resistantEnterococcus faecalisstrain (EFs11496). Daptomycin MICs were 4, 2, and 0.5 μg/ml for EFm11499, 09-184D1051, and EFs11496, respectively. Bactericidal activity, defined as a ≥3 log10CFU/g reduction from the initial colony count, was demonstrated against all three isolates with all doses of daptomycin; however, bactericidal activity was not sustained with the daptomycin 6- and 8-mg/kg/day regimens. Linezolid was bacteriostatic against EFm11499 and displayed no appreciable activity against 09-184D1051 or EFs11496. Concentration-dependent killing was displayed with more sustained reduction in colony count (3.58 to 6.46 and 5.89 to 6.56 log10CFU/g) at 96 h for the simulated regimen of daptomycin at doses of 10 and 12 mg/kg/day, respectively (P≤ 0.012). NoE. faeciummutants with reduced susceptibility were recovered at any dosage regimen; however, theE. faecalisstrain developed reduced daptomycin susceptibility with daptomycin at 6, 8, and 10 but not at 12 mg/kg/day. Daptomycin displayed a dose-dependent response against three VRE isolates, with high-dose daptomycin producing sustained bactericidal activity. Further research is warranted.

scholarly journals Antibiotic Intervention Affects Maternal Immunity During Gestation in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Marilen Benner ◽  
Alejandro Lopez-Rincon ◽  
Suzan Thijssen ◽  
Johan Garssen ◽  
Gerben Ferwerda ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Treated Group ◽  
Mesenteric Lymph Nodes ◽  
Negative Effects ◽  
T Helper 17 ◽  
Immunological Parameters ◽  
Maternal Immunity ◽  
Immune Parameters ◽  
Ensemble Strategy ◽  
The Impact

BackgroundPregnancy is a portentous stage in life, during which countless events are precisely orchestrated to ensure a healthy offspring. Maternal microbial communities are thought to have a profound impact on development. Although antibiotic drugs may interfere in these processes, they constitute the most frequently prescribed medication during pregnancy to prohibit detrimental consequences of infections. Gestational antibiotic intervention is linked to preeclampsia and negative effects on neonatal immunity. Even though perturbations in the immune system of the mother can affect reproductive health, the impact of microbial manipulation on maternal immunity is still unknown.AimTo assess whether antibiotic treatment influences maternal immunity during pregnancy.MethodsPregnant mice were treated with broad-spectrum antibiotics. The maternal gut microbiome was assessed. Numerous immune parameters throughout the maternal body, including placenta and amniotic fluid were investigated and a novel machine-learning ensemble strategy was used to identify immunological parameters that allow distinction between the control and antibiotic-treated group.ResultsAntibiotic treatment reduced diversity of maternal microbiota, but litter sizes remained unaffected. Effects of antibiotic treatment on immunity reached as far as the placenta. Four immunological features were identified by recursive feature selection to contribute to the most robust classification (splenic T helper 17 cells and CD5+ B cells, CD4+ T cells in mesenteric lymph nodes and RORγT mRNA expression in placenta).ConclusionIn the present study, antibiotic treatment was able to affect the carefully coordinated immunity during pregnancy. These findings highlight the importance of inclusion of immunological parameters when studying the effects of medication used during gestation.

scholarly journals Показники неспецифічної резистентності однорічок коропа за інвазії ектопаразитами

2013 ◽  
pp. 104-106
Author(s):  
Ю. В. Лобойко
Keyword(s):  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Lernaea Cyprinacea ◽  
Carp Serum

Наведено дані щодо лізоцимної і бактерицидноїактивності сироватки крові та фагоцитарноїактивності нейтрофілів за різної інтенсивностіінвазії ектопаразитами. Встановлено зниженнялізоцимної, бактерицидної та фагоцитарної ак-тивності сироватки крові коропів за ураженняектопаразитами Lernaea cyprinacea та Dactylogyrusvastator, порівняно з клінічно здоровимирибами. За високої інвазії ектопаразитами (>0,26лерней/г м.т. та >0,53 дактилогірусів /г м.т.)спостерігали вірогідне зниження показників неспе-цифічної резистентності однорічок коропа. The data on lyzocymic and bactericidal activity of serum and blood phagocytic activity ofneutrophils by different intensity of infestation by ectoparasites. The decrease lyzocymic, bactericidal andphagocytic activity of carp serum by lesions ectoparasites Lernaea cyprinacea and Dactylogyrus vastatorcompared with clinically healthy fish.

scholarly journals Influence of the prebiotic Bio-active on natural resistance, safety and productivity of young pigs

2021 ◽  
pp. 37-49
Author(s):  
V. Lyasota ◽  
V. Malyna ◽  
L. Bondarenko ◽  
V. Bolokhovska ◽  
Y. Balatsky ◽  
...  
Keyword(s):  
Body Weight ◽  
Veterinary Medicine ◽  
T Lymphocytes ◽  
Research Methods ◽  
B Lymphocytes ◽  
Optimal Dose ◽  
Immunocompetent Cells ◽  
Young Pigs ◽  
Immunobiological Reactivity ◽  
First Time

The primary task of the livestock industry is to provide the population with high-quality food products, and the industry with raw materials. For this, it is necessary to ensure a high level of productivity and safety of young pigs. Studies carried out in recent years indicate an increase in the incidence of gastrointestinal diseases among young farm animals, leading to a decrease in the immunobiological reactivity of the piglets' organism and significant damage to farms. When performing the research, we used zootechnical, zoohygienic, biochemical, immunological and variational-statistical research methods. Research work was carried out during 2010-2018 at the Department of Animal Hygiene and Fundamentals of Veterinary Medicine of the Bila Tserkva National Agrarian University. Scientific and economic experiments and production tests were carried out in the farms of Progress LLC (Uzin, Bila Tserkva district) and Denisenko LLC (Skvirsky district, Kyiv region). Experimental studies were carried out in the Problem Laboratory of Immunology, Department of Animal Hygiene and Fundamentals of Veterinary Medicine, interfaculty laboratory of biochemical and histochemical research methods. For the first time, the prebiotic Bio-active was used as a feed additive in the diet of young pigs on growing at industrial keeping, its positive effect on the safety, productivity, physiological and immunological state of their body was proved. For the first time, the optimal dose of the prebiotic Bio-active for growing pigs was established and a method of its use was developed. The optimal dose of the prebiotic Bio-active for growing young pigs is 5 g (5x107 CFU) per 10 kg of body weight when fed with compound feed once a day for 30 days, which contributes to an increase in the average daily weight gain of piglets by 17.2 % (P <0.01) and 16.6 % (P <0.05) on the 30th and 60th days of the study, respectively. Feeding pigs with Bio-active promotes an increase in the content of total blood serum protein by 7.2 % (P <0.05), albumin – by 5.2 %, γ-globulins – by 6.3 %, an increase in AST activity – by 23, 1 % (P <0.05) and ALT – by 22.4 % (P <0.01). In the peripheral blood of rearing pigs under the influence of the prebiotic Bio-active, the proliferation, differentiation and specialization of immunocompetent cells increase: an increase in the total number of T-lymphocytes by 5.43 % (P <0.05), B-lymphocytes by 2.85 %, a decrease in the content of 0-lymphocytes – by 8.29 % (P <0.05). The number of medium-avid T-lymphocytes also increases – by 9.19 % (P <0.05) and B-lymphocytes – by 10.28 % (P <0.05) due to a decrease in the level of low-avid immunocompetent cells. The use of the prebiotic Bio-active in an optimal dose promotes the activation of metabolic processes, antigen-nonspecific immunity and an increase in the body weight of pigs. Key words: industrial pig breeding, rearing young pigs, housing conditions, gastrointestinal canal, prebiotic, immunobiological reactivity, metabolism, safety.

scholarly journals THE EFFECT OF «ARCUSIT» ON BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS BLOOD SERA IN SILVER-BLACK FOX (SILVER-BLACK FOX)

2021 ◽  
Vol 247 (3) ◽  
pp. 192-197
Author(s):  
I.I. Okulova ◽  
◽  
I.A. Domsky ◽  
Yu.A. Berezina ◽  
Z.N. Beltyukova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Lysozyme Activity ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Immunological Parameters ◽  
Nonspecific Resistance ◽  
Experimental Group

The use of «Arkusit» at a dose of 20 μg / kg of body weight indicates a beneficial effect on the factors of nonspecific resistance of the silver-black fox. The concentration of alkaline phosphatase in the blood serum in the experimental group decreased by 1.8 times (P <0.001), the ALT index - by 1.2 (P <0.05). The concentration of glucose in the experimental group increased 2 times (P <0.05) compared with the control group. In animals of the experimental group, the lysozyme activity of blood serum increased 1.3 times (P <0.05), the OPR reaction and the phagocytic index - 1.4 times (P <0.05), the bactericidal activity of blood serum - 1.8 times (P <0.05). «Arcusit» stimulates the synthesis of gamma globulins, increases the opsonophagocytic, bactericidal activity of blood serum. Thus, the analysis of experimental data indicates that the drug «Arcusit» has a pronounced immunostimulating effect, providing the activation of factors of nonspecific resistance of the body of the silver-black fox.

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