scholarly journals Біохімічні особливості формування поросят першої доби життя

Author(s):  
І. І. Панікар

Рівень майже всіх показників клінічного метаболі-зму, а особливо білкових фракцій, у сироватці крові поросят у «домолозивному» періоді значно відріз-няється від показників поросят першої доби життя, які отримували молозиво. Зміна показни-ків білкового обміну пов’язана з надходженням в організм білків молозива. Підвищення активності АлАт, ЛДГ рівня вмісту глюкози, концентрації загального білірубіну за зниження лужної фосфа-тази та сечовини в крові свідчить про стресовий стан організму, що можна пояснити стресовим фактором, отриманим організмом під час та відразу після народження. The level of many indicators of clinical metabolism, especially protein fractions in the blood serum of piglets in the «pre-colostrum» period significantly differ from that of the piglets of the first day of life fed by colostrum. Changing the parameters of protein metabolism is associated with intake of colostrum proteins. Increase of ALT, LDH levels of glucose, serum total bilirubin with a decrease of alkaline phosphatase and urea in the blood indicates a stressful condition of the body, which can be explained by a stress factor, obtained by the body during and immediately after birth.

2020 ◽  
Vol 3 (2) ◽  
pp. 42-46
Author(s):  
V. G. Stoyanovskyy ◽  
M. O. Shevchuk ◽  
I. A. Kolomiiets ◽  
V. A. Kolotnytskyy

The body of  broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.


2020 ◽  
Vol 8 (2) ◽  
pp. 205-212
Author(s):  
Tunji Akande ◽  
Timothy Olugbenga Ogundeko ◽  
Solomon Oyewale Opajobi ◽  
Samaila Garba Yaura ◽  
Isiaiah Iorkyase Idyu ◽  
...  

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.


2020 ◽  
Vol 8 (2) ◽  
pp. 205-212
Author(s):  
Tunji Akande ◽  
Timothy Olugbenga Ogundeko ◽  
Solomon Oyewale Opajobi ◽  
Samaila Garba Yaura ◽  
Isiaiah Iorkyase Idyu ◽  
...  

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.


2021 ◽  
Author(s):  
Roman Kryukovsky ◽  
Aleksei Alekseev ◽  
Igor Glamazdin ◽  
Natalia Sysoeva ◽  
Lev Ananyev ◽  
...  

White-tailed deer have been introduced into Russia, andthey are exposed to stress factors. The aim of this article was to study the clinical and biochemical blood parameters of white-tailed deer under stress. The experiment was conducted on 24 adult animals. Under stress the content of the red blood cells and hemoglobin increased, and the content of the leukocytes decreased with an increase in the share of segmented neutrophils and a decrease in the share of lymphocytes. In the blood serum, the content of glucose, total bilirubin, creatinine, urea, cholesterol, and enzymes KFK, LDH and GGT increased, and the content of calcium, phosphorus, magnesium and iron decreased. Keywords: odocoileus virginianus, adaptation, introducers, stress factor, immunosuppression


Author(s):  
N. I. Burmas ◽  
L. S. Fira ◽  
P. H. Lyhackyy

<p>Background. Currently, the growing incidence of toxic lesions of the liver is associated with<br />industrial chemicalization and uncontrolled use of hepatotoxic drugs in everyday life. There are about<br />one thousand drugs with high or low hepatotoxicity, such as anti-TB drugs.<br />Objective. In this research we studied the intracellular enzymes activity and bile formation function<br />of the liver in rats of different ages in cases of tuberculostatic (isoniazid and rifampicin) affection and<br />chromium (potassium dichromate) intoxication.<br />Methods. The experimental affection of rats of different ages was performed by combined injection<br />of hexavalent chromium compounds (a solution of potassium dichromate, 3 mg/kg), isoniazid<br />(0.05 g/kg) and rifampicin (0.25 g/kg). On the 7th and 14th days the rats were injected with enterosorbent<br />Sorbex (150 mg/kg). Enzyme markers activity of the liver was evaluated due to alanine and aspartate<br />aminotransferases (ALT and AST) and alkaline phosphatase (ALP) rates. Bile formation function of the<br />liver was evaluated by total bilirubin and bile acids content in blood.<br />Results. The disorders in hepatocytes plasma membranes permeability were defined by the<br />increased rates of ALT, AST and alkaline phosphatase in blood serum which were decreased in the<br />liver. It was determined that total bilirubin and bile acids content in blood serum of the affected<br />animals increased. It influenced hepatocytes excretion in bile capillaries and caused cholestasis and<br />revenues decrease in bile.<br />Conclusions. The most significant metabolic disorders in cases of chrome-isoniazid-rifampicin<br />affection were defined in immature and senior animals in comparison with mature animals.<br />KEY WORDS: isoniazid, rifampicin, hexavalent chromium compounds, liver enzymes,<br />bile formation.</p>


Author(s):  
L.A. Nikanova ◽  
◽  
E.N. Kolodina ◽  
R.A. Rykov ◽  
◽  
...  

This article presents the results of studies conducted on piglets with the inclusion of natural feed additives in the diet in order to prevent and correct metabolism. The first experimental group of piglets received a total diet consisting of fullfledged compound feed, a complex micro-feed product consisting of blue-green algae Spirulina, dihydroquercetin and organic iodine, the second experimental group additionally received Spirulina and dihydroquercetin. The introduction of these natural feed additives into the diet of piglets helped to increase the adaptive ability , correction and prevention of metabolic disorders in the body. In these studies, dacha feed supplements prevented hyperbilirubinemia. As a result, the concentration of total bilirubin in the blood serum of pigs of the first experimental group was 1% lower, in the second experimental group it was 13,7% lower than in the control group. They also had a positive effect on the functional state of the liver, as evidenced by a lower content of AlAT and AsAT activity in the blood serum compared to those in piglets of the control group with a close cholesterol-forming fraction. The animals of the experimental group were less ill and the safety of this group was 100%, in the control group it was 90%. The average daily increase in live weight of pigs for 90 days in the first experimental group was 1% higher than in the control group, and in the second experimental group it was 21,7% higher.


Author(s):  
В. В. Мельничук ◽  
О. С. Назаренко ◽  
С. І. Назаренко

Наведені результати гематологічних дослідженьщодо визначення впливу збудника трихурозу свинейна біохімічні показники сироватки крові інвазованихтварин. Встановлено, що паразитування трихурисівв організмі поросят призводить до достовірногозниження вмісту загального білка, альбумінів і зрос-тання вмісту загального білірубіну, непрямого біліру-біну й активності ферментів у сироватці крові.Отримані дані свідчать про залучення у патологіч-ний процес паренхіми печінки, серцевого м'яза і гла-денької мускулатури кишечника. The results of hematological studies to determine the impact of the pathogen of trichurosis of pigs on blood serum biochemical parameters infested animals are presented. We established that trichurises’ parasites in the body of pigs leads to a significant reduction of total protein, albumin and increase of total bilirubin, indirect bilirubin and enzyme activity in blood serum. These data suggest about involvement of liver parenchyma in the pathological process, cardiac muscle and smooth muscles of the intestine.


2019 ◽  
Vol 60 (11) ◽  
pp. 48-55
Author(s):  
Mikhail Y. Vlasov ◽  
◽  
Elena V. Pisareva ◽  
Yulia V. Golub ◽  
Pyotr P. Purygin ◽  
...  

Collagen is an intercellular substance of connective tissue, it plays an important role in the physiological processes of the body. Collagen consists of three polypeptide chains twisted in a spiral and in order to stabilize this structure, hydroxyproline is needed, its hydroxyl groups are involved in the formation of hydrogen bonds between the chains of polypeptides. The peculiarity of collagen is that 14% of the amino acids it contains are hydroxyproline, an amino acid that is not contained in other proteins. In this regard, the content of hydroxyproline in blood serum was used to assess the metabolism of collagen in the body. We determined the content of hydroxyproline fractions in blood serum. Calcium, fibrinogen, erythrocyte settling rate and alkaline phosphatase level in children and adults with joint disease were also determined. The control group included healthy children and adults. The total hydroxyproline content in children with the disease was 9-11% lower than in healthy children. In adults with joint diseases, the total hydroxyproline content was 11% higher than in healthy patients. In children and adults with rheumatic diseases, the peptide-bound hydroxyproline content increased, while the protein-bound hydroxyproline content decreased in comparison with the control group control parameters. No significant changes in the content of calcium and fibrinogen were noted, but the level of alkaline phosphatase and the rate of erythrocyte sedimentation was increased, which is typical for people with inflammatory diseases.


Author(s):  
I. A. Bandas ◽  
M. I. Kulitska ◽  
M. M. Korda

Nanoparticles are known to facilitate transport of chemicals and medications through membrane barriers into cells. This results in the raising of toxic effect when two contaminants act on the body at the same time. The aim of this study was to determine how silicon dioxide (SiO2) nanoparticles affect the hepatotoxic properties of lead. Alanine and aspartate transaminases, alkaline phosphatase activities, as well as total protein, total bilirubin, creatinine and urea contents were measured in rat blood plasma. It was shown that SiO2 nanoparticles did not significantly affect the above listed markers. Administration of lead acetate to the animals caused cytolysis of hepatocytes, as evidenced by significant increase of alanine and aspartate transaminases, alkaline phosphatase activities, along with significant decrease of total plasma protein and increase of total bilirubin levels. The increase of aminotransferases and alkaline phosphatase activities in animals that received both SiO2 nanoparticles and lead acetate was significant comparedto the animals that received only lead acetate. This suggests that SiO2 nanoparticles, which are normally present inthe environment and food products as contaminants, can enhance the negative hepatotoxic effects of lead.


2017 ◽  
Vol 51 (3) ◽  
Author(s):  
Florence Giannina F. San Juan ◽  
Annavi Marie G. Villanueva ◽  
Germana Emerita V. Gregorio ◽  
Catherine Lynn T. Silao

Objective. To determine if the CD14/-159 and the TNFα/-308 single nucleotide polymorphisms (SNPs) are associated with the development of Idiopathic Neonatal Hepatitis (INH) in Filipino children. Methods. Genomic DNA from 33 patients diagnosed with INH and 33 age- and sex-matched controls, children without any liver disease, were recruited. Baseline serum total bilirubin (TB), direct bilirubin (DB), and alkaline phosphatase (ALP) of the patients were obtained from their medical records. Genotypes for CD14/159 and TNFα/-308 were determined via PCR and direct sequencing. Results. No significant difference was seen between the frequency of the CD14/-159 T allele (p=0.86) nor the TNFα/-308 A allele (p=0.62) between INH patients and controls. There was also no significant difference between the genotypic distribution of the INH and control populations for both CD14/-159 (p=0.54) and TNFα/-308 (p=0.62). There were also no significant differences noted between the different genotypes of CD14/159 and TNFα/-308 and levels of alkaline phosphatase (p=0.65, p=0.91), total bilirubin (p=0.89, p=0.75), and direct bilirubin (p=0.93, p=0.68). Conclusion. In this preliminary study, CD14/-159 and TNFα/-308 showed no association with the development of INH among Filipinos.


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