PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

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AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽

10.33751/ekol.v19i1.1660 ◽

2020 ◽

Vol 19 (1) ◽

pp. 34-38

Author(s):

Siti Warnasih ◽

Diana Widiastuti ◽

Uswatun Hasanah ◽

Laksmi Ambarsari ◽

Purwantiningsih Sugita

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soxhlet Extraction ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Palm Fruit ◽

Ic50 Value ◽

Dpph Method ◽

Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.433 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41-47

Author(s):

Poppy Anjelisa Zaitun Hasibuan ◽

Mardiana

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Ethanol Extract ◽

Positive Control ◽

Hexane Extract ◽

Phytochemical Screening ◽

Ic50 Value ◽

Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)

Jurnal Sains Natural ◽

10.31938/jsn.v11i2.309 ◽

2021 ◽

Vol 11 (2) ◽

pp. 79

Author(s):

Bhayu Gita Bhernama ◽

Witri Maulidy Ayu ◽

Cut Nuzlia

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Vitamin C ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Positive Control ◽

Red Seaweed ◽

Oxidation Reactions ◽

Ic50 Value ◽

Dpph Method

Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH

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ANTIOXIDANT ACTIVITY AND CARDIOPROTECTIVE ACTIVITY OF BANGUN-BANGUN LEAVES (PLECTRANTHUS AMBOINICUS LOUR.) ETHANOLIC EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.26907 ◽

2018 ◽

Vol 11 (9) ◽

pp. 165

Author(s):

Modesta Harmoni Tarigan ◽

Urip Harahap ◽

Aminah Dalimunthe ◽

Nerdy Nerdy

Keyword(s):

Antioxidant Activity ◽

Troponin T ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Heart Tissue ◽

Positive Control ◽

Control Group ◽

Activity Test ◽

Ic50 Value ◽

Plectranthus Amboinicus

Objectives: The objectives of this study were to determine the antioxidant activity and cardioprotective activity of bangun-bangun leaves ethanolic extract.Methods: Bangun-bangun leaves ethanolic extract was obtained by maceration process. The antioxidant activity test was performed by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging method with various concentrations of extract. The absorbance was measured by visible spectrophotometric method and calculated the inhibitory concentration (IC50) value for antioxidant activity analysis. Cardioprotective activity test was performed by measuring the cardiac troponin T (cTnT) level, creatine kinase-muscle/brain (CK-MB) level, and histology of the heart tissue. Animals induced with doxorubicin at the 8th day and the 9th day, bangun-bangun leaves ethanolic extract was administered from the 1st day to the 9th day with various doses of extract.Results: Bangun-bangun leaves ethanolic extract had IC50 value of 57.79 μg/mL. Difference dose of bangun-bangun leaves ethanolic extract shows difference cardioprotective activity. Bangun-bangun leaves ethanolic extract at dose 300 mg/kg bw did not differ significantly to the positive control group and normal group. The higher the dose of an extract the greater the decrease in cTnT and CK-MB levels and increase protection against heart damage.Conclusion: Bangun-bangun leaves ethanolic extract had strong antioxidant and had cardioprotective activity.

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SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽

10.35799/pha.8.2019.29297 ◽

2019 ◽

Vol 8 (2) ◽

pp. 315

Author(s):

Selin Widjaya ◽

Widdhi Bodhi ◽

Adithya Yudistira

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Brine Shrimp ◽

Ethanol Extract ◽

Brine Shrimp Lethality ◽

Phytochemical Content ◽

Lc50 Value ◽

Ic50 Value ◽

Dpph Method ◽

Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol. Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

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Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.13662 ◽

2020 ◽

Vol 6 (3) ◽

pp. 198-205

Author(s):

Nur Faidah ◽

Nurhaeni ◽

Ahmad Ridhay ◽

Jusman ◽

Abd. Rahman Razak ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Allium Cepa ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Root Extract ◽

Polar Solvents ◽

Ic50 Value ◽

Dpph Method ◽

Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.51 ◽

2021 ◽

Vol 3 (2) ◽

pp. 53-61

Author(s):

Angga Crystal Loasana Yami ◽

Irmanida Batubara ◽

Kholis Abdurachim Audah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Vibrio Cholerae ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Antibacterial Activities ◽

Diffusion Method ◽

Ic50 Value ◽

Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

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Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

Jurnal Kesehatan Masyarakat ◽

10.15294/kemas.v15i3.19521 ◽

2020 ◽

Vol 15 (3) ◽

pp. 382-389

Author(s):

Mirwa Adiprahara Anggarani ◽

Roy Januardi Irawan

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Ethyl Acetate ◽

Cell Structure ◽

Oxygen Species ◽

Meat Extract ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Reactive Molecule

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

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Aktivitas antioksidan krim kombinasi ekstrak Eucheuma Cottonii Sumbawa dan ekstrak Citrus lemon L. impor dengan metode DPPH

Health Sciences and Pharmacy Journal ◽

10.32504/hspj.v3i1.95 ◽

2019 ◽

Vol 3 (1) ◽

pp. 29

Author(s):

Dwi Kurniawati Sambodo ◽

Nandia Arlesia

Keyword(s):

Antioxidant Activity ◽

Negative Impact ◽

Positive Control ◽

Test Results ◽

Negative Effects ◽

High Exposure ◽

Ic50 Value ◽

Dpph Method ◽

Qualitative Test ◽

Eucheuma Cottonii

Cerrenty, The high exposure for free radicals has a negative impact on the health and aesthetics of the skin, especially facial skin, because it requires cosmetic that contain antioxidants to counteract and reduce the negative effects of it. Eucheuma cottonii and Citrus lemon L have bioactive compounds that have the potential as antioxidants. This research was conducted to find out antioxidant activity and the most optimal formula of the three cream formulas by DPPH method. Eucheuma cottonii and Citrus lemon L peel were extracted by remaseration method. The cream is formulated in 3 formulas with a ratio of extracts of 1: 2, 2: 1, and 1: 1 in 100 grams of cream with positive control of vitamin C. The three formulas were tested qualitatively and quantitatively by DPPH method with spectrophotometry visible at maximum wavelength 517 nm uses IC50 value. Qualitative test shown that the three cream formulas had antioxidant activity by DPPH damping which were characterized by reduced intensity of purple color. Quantitative test results in IC50 value formula 1 was 11251.5 ppm, formula 2 was 8592.2 ppm, and 10025.5 ppm for formula 3. From too concluded that the three creams have antioxidant activity and formula 2 has the most optimal antioxidant than 2 other formulas

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Uji Aktivitas Antioksidan Hidrolisat Protein Kerang Pasir (Semele cordiformis) dengan Metode DPPH (Antioxidant Activity of Protein Hydrolisate from Semele cordiformis Using DPPH Methode)

MEDULA ◽

10.46496/medula.v7i2.11969 ◽

2020 ◽

Vol 7 (2) ◽

Author(s):

Ummi Kalsum ◽

Indria Hafizah ◽

Pranita Aritrina ◽

Sulastrianah Sulastrianah

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Protein Hydrolysate ◽

Protein S ◽

Positive Control ◽

Control Group ◽

Control Group Design ◽

Ic50 Value ◽

Dpph Method ◽

Post Test

Background: Sand shell (Semele cordiformis) is commonly found in South East Sulawesi marine area. This species traditionally used for treatment of several disease. Nevertheless, there is less publication about bioactivity of S. cordiformis as asource of drugs. Purpose: The aims of this research is to determine the activity of protein hydrolysate of S. cordiformis as an antioxidant. Methods: This research was conducted by pre-experimetal study with post test only control group design. Antioxidant activity was measure by DPPH method and spectrophotometry. The sample was hydrolyzed by using papain enzyme. The sample was made in to concentration 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm and vitamin C was used as positive control. The IC50 was used to measure antioxidant activity. Results: Concentration of 200 ppm showed 36,68% of inhibition percentage, 400 ppm showed 47,69%, 600 ppm showed 60,19%, 800 ppm showed 65,63%, and 1000 ppm showed 72,69 %. The IC50 value of was obtained for 453,777 ppm. Conclusion: The protein hydrolysate of S. cordiformis have an antioxidant activity and the hydrolisat concentration is directly proportional with inhibition activity.Keyword: antioxidant, protein hydrolysate, Semele cordiformis ABSTRAKPendahuluan: Kerang pasir atau Semele ccordiformis merupakan jenis kerang yang banyak ditemukan di perairan Sulawesi Tenggara. Kerang ini merupakah salah satu jenis kerang yang telah dimanfaatkan secara turun-temurun oleh masyarakat lokal untuk pengobatan. Meskipun demikian, belum ditemukan publikasi ilmiah mengenai bioaktifitas S. ccordiformis dalam bidang kesehatan. Tujuan: Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan hidrolisat protein S. cordiformis. Metode: Penelitian menggunakan desain pra-eksperimental dengan pendekatan post-test only control group. Uji aktivitas antioksidan menggunakan metode DPPH dengan prinsip spektrofotometri. Sampel dari penelitian ini adalah S. cordiformis yang diperoleh dari perairan Pulau Bokori, Kabupaten Konawe, Provinsi Sulawesi Tenggara yang kemudian dihidrolisis menggunakan enzim papain. Sampel dibuat menjadi beberapa konsentrasi yaitu 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm dan vitamin C digunakan sebagai kontrol positif. Nilai IC50 digunakan untuk mengetahui aktifitas antioksidan. Hasil: Konsentrasi 200 ppm menghasilkan persen inhibisi sebesar 36,68%, 400 ppm sebesar 47,69%, 600 ppm sebesar 60,19%, 800 ppm sebesar 65,63%, dan 1000 ppm sebesar 72,69 %. Nilai IC50 yang diperoleh adalah 453,777 ppm. Simpulan: hidrolisat protein S. cordiformis memiliki aktivitas antioksidan dengan konsentrasi hidrolisat berbanding lurus terhadap aktifitas inhibisi.Kata Kunci: antioksidan, hidrolisat protein, Semele cordiformis

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