Antioxidant, anticancer, antibacterial activities and GCMS analysis of aqueous extract of pulps of Aegle marmelos (L.) Correa

Aegle marmelos (Bael) is an important medicinal plant which belongs to family Rutaceae. Leaves, fruit, stem, bark of this plant is used because of its medicinal properties like astringent, antidiarrheal, antipyretic, antiinflammatory activities. Compounds purified from A.marmelos have been proven biologically active against various several major diseases like cancer, diabetes, cardiovascular diseases. The aim of the present study was to evaluate the antioxidant, anticancer and antibacterial activities of aqueous extract of A. marmelos and to identify the bioactive compounds by GC-MS analysis. Antioxidant activities such as DPPH radical, ABTS·+ radical cation, Fe3+ reducing power and phosphomolybdenum reduction assays were carried out for aqueous extract. The maximum DPPH radical and ABTS·+ radical cation scavenging activities were 60.70±4.24% at 300 µg/mL and 95.77±6.70% at 30 µg/mL concentrations. The IC50 values of DPPH˙ radical and ABTS·+ radical cation scavenging activities were 183.58 µg/mL and 10.81 µg/mL concentration. The maximum reduction of Fe3+ and Mo6+ were 52.05±3.64% and 87.81±6.19% at 120 µg/mL concentration and the IC50 values were 102.83 µg/mL and 32.76 µg/mL concentration. The anticancer activity was carried out by MTT assay method, which showed the IC50 value of 47.92 µg/mL concentration

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Antioxidant and Antibacterial Activities of ethanol fruit extract of Cherry Plum - Prunus cerasifera Ehrh.

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i1-s.3851 ◽

2020 ◽

Vol 10 (1-s) ◽

pp. 45-50 ◽

Cited By ~ 1

Author(s):

K Saraswathi ◽

C Sivaraj ◽

P Arumugam

Keyword(s):

Radical Cation ◽

Functional Foods ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Dpph Radical ◽

Herbal Products ◽

Prunus Cerasifera

Foods that have disease-preventing potential are designated as functional foods. Functional foods are foods that provide health benefits beyond basic nutrition. A large number of antioxidants, both nutritive and nonnutritive, occur in foods. The potency of antioxidants present in foods in vivo will depend not only on their levels in the foods but also on their bioavailability, that is, the extent to which the active forms of antioxidants are released from the food and absorbed through the gut. Most of phytochemicals, components of food, beverages, and herbal products are often reported in literature as “nutraceutical”, emphasizing their health promoting properties, including the prevention and treatment of pathologies like cancer, cardiovascular diseases, neural disorders, and Alzheimer’s disease. Phytochemicals are virtually present in all the fruits, vegetables, pulses/legumes and grains which are commonly consumed, so it is quite easy to incorporate them in our daily diet. Current research studies were carried out for evaluating the antioxidant and antibacterial activities of ripe fruits of ethanol extract of Prunus cerasifera. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for ripe fruits of ethanol extract of Prunus cerasifera. The maximum DPPH˙ radical and Superoxide (O2.-) radical scavenging activities were 82.11±0.42% and 46.26±0.28% at 120 µg/mL concentration and the IC50 values were 45.40 µg/mL and 129.70 µg/mL concentrations respectively. The maximum ABTS●+ radical cation scavenging activity was 88.64±0.43% at 30 µg/mL concentration and the IC50 value was 10.09 µg/mL concentration respectively. The maximum Mo6+ reduction and Fe3+ reduction were 86.29±0.29% and 79.82±0.20% at 120 µg/mL concentration and the RC50 values were 20.45 µg/mL and 24.21 µg/mL concentrations respectively. The antibacterial activity of ripe fruits of ethanol extract of Prunus cerasifera showed maximum zone of inhibition of 20 mm for Bacillus subtilis at 500 µg/mL concentration. Keywords: Antioxidant, Superoxide (O2.-) radical, ABTS●+ radical cation, Fe3+ reduction and phenolic compounds.

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Phytochemical Analysis, Antioxidant Activities and GC-MS Profiling of Ethanol Bract Extract of Musa paradisiaca L.

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4-s.4273 ◽

2020 ◽

Vol 10 (4-s) ◽

pp. 167-175

Author(s):

Perumal Arumugam ◽

Krishna Saraswathi ◽

Dhanasekar Dhivya ◽

Chandrasekaran Sivaraj

Keyword(s):

Nitric Oxide ◽

Radical Cation ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antioxidant Compounds ◽

Phytochemical Analysis ◽

Dpph Radical ◽

Traditional System ◽

Musa Paradisiaca ◽

Ic50 Values

In recent year’s trend towards authentication, standardization and quality assurance of selected medicinal plants has been rapid increase for potential therapeutic significance. Despite the modern techniques, identification of plant drug by pharmacognostic study is more reliable. In Ayurveda, a traditional system of medicine Musa Paradisiaca is commonly used in Asthma, Diabetes, Anthelmintic, Hypertension, Insomnia, Snake bite. The whole plant as well as specific parts (leaves, ripe and unripe fruits, stems) of plant extract and its active constituents have been used for the treatment of large number of human ailments. Phytochemicals are virtually present in all the fruits, vegetables, pulses/legumes and grains which are commonly consumed, so it is quite easy to incorporate them in our daily diet. Current research studies were carried out for evaluating the antioxidant activities of ethanol bract extract of Musa paradisiaca. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, Nitric Oxide (NO) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for ethanol bract extract of Musa paradisiaca. The maximum DPPH˙ radical, Superoxide (O2.-) radical and Nitric Oxide (NO) radical scavenging activities were 52.05±0.38%, 57.71±0.45% and 49.63±0.32% at 120 µg/mL concentration and the IC50 values were 68.47 µg/mL, 71.37 µg/mL and 93.94 µg/mL concentrations respectively. The maximum ABTS●+ radical cation scavenging activity was 84.28±0.13% at 30 µg/mL concentration and the IC50 value was 10.04 µg/mL concentration respectively. The maximum Mo6+ reduction and Fe3+ reduction were 45.38±0.40% and 57.06±0.32% at 120 µg/mL concentration and the RC50 values were 121.44 µg/mL and 85.43 µg/mL concentrations respectively. Z-2-Tridecen-1-ol, Nonadecane-2,4-dione, a-Ketostearic acid, 2,5-Furandione, dihydro-3-(2-tetradecenyl)- were found to be the potent antioxidant compounds detected from GC-MS analysis. Keywords: Antioxidant, phenolic compounds, Nitric Oxide (NO) radical, ABTS●+ radical cation, Fe3+ reduction and a-Ketostearic acid.

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Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

Phytothérapie ◽

10.3166/phyto-2021-0282 ◽

2021 ◽

Author(s):

E.K. Soltani ◽

K. Zaim ◽

K. Mokhnache ◽

N. Haichour ◽

S. Mezaache-Aichour ◽

...

Keyword(s):

Aqueous Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antibacterial Properties ◽

Natural Antioxidants ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Biological Properties ◽

Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

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Efficient Synthesis of Novel N-[4-Methyl-3-(4-(5-phenyl-1,3,4-oxadiazol-2-yl)phenyl)thiazol-2(3H)-ylidene]benzamide Hybrid Ring System as Potential Antibacterial Agents

Medicinal Chemistry ◽

10.2174/1573406417666210923103209 ◽

2021 ◽

Vol 17 ◽

Author(s):

Hummera Rafique ◽

Aamer Saeed ◽

Muhammad Naseem ◽

Tauqeer Riaz ◽

Fouzia Perveen ◽

...

Keyword(s):

Bacterial Resistance ◽

Antioxidant Activities ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Structural Features ◽

Ring System ◽

Biologically Active ◽

Thiazole Ring ◽

Spectroscopic Techniques ◽

Hybrid Ring

Background: Heterocyclic compounds display versatile biological applications, so the aim of this paper was to prepare biologically important heterocycles with enhanced bacterial resistance and to evaluate for their various structural features that are responsible for their biological properties. Objective: The objective was to synthesize bacterial resistance compounds with enhanced antibacterial properties. Method: Ester moiety containing thiazole ring was converted into its hydrazide derivatives. These heterocyclic derivatives were cyclized into another ring oxadiazole; hence a hybrid ring system of two biologically active rings was prepared. Result: All the synthesized compounds were characterized by spectroscopic techniques and were screened for their antibacterial potential; they possess significant antibacterial activities. Conclusion: New hybrid heterocyclic ring systems were synthesized by cyclization of hydrazide derivatives by adopting two step strategy in good yields. All the synthesized compounds were evaluated for their antioxidant activities; they showed moderate to significant activities. QSAR and Molecular docking studies were performed to determine the mode of interaction. Experimental and computational data is in accordance with the determined antibacterial activities.

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Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

The Scientific World JOURNAL ◽

10.1155/2018/7470384 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Kenza Ammor ◽

Dalila Bousta ◽

Sanae Jennan ◽

Bahia Bennani ◽

Abdellah Chaqroune ◽

...

Keyword(s):

Antibacterial Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antibacterial Activities ◽

Diffusion Method ◽

Antioxidant Power ◽

Negative Effect ◽

Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

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Antioxidant Activities of Flavonoids from Canavalia maritime

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.834-836.577 ◽

2013 ◽

Vol 834-836 ◽

pp. 577-581

Author(s):

Jian Chao Deng ◽

Gai Gai Niu ◽

Lai Hao Li ◽

Xian Qing Yang ◽

Yong Chuan Deng ◽

...

Keyword(s):

Solvent Extraction ◽

Hydroxyl Radical ◽

High Speed ◽

Superoxide Radical ◽

Antioxidant Activities ◽

Reducing Power ◽

Dpph Radical ◽

Inhibitory Effects ◽

Counter Current

Flavonoids was isolated from canavalia maritime by accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC). The antioxidant activities of flavonoids were investigated in vitro and evaluated by IC50. The results indicated that flavonoids possessed obvious reducing power and significant inhibitory effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggest that canavalia maritime flavonoids could be a suitable natural antioxidant for humans.

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The Study of Interactions between Active Compounds of Coffee and Willow (Salixsp.) Bark Water Extract

BioMed Research International ◽

10.1155/2014/386953 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Agata Durak ◽

Urszula Gawlik-Dziki

Keyword(s):

Antioxidant Activities ◽

Raw Materials ◽

Water Extract ◽

Reducing Power ◽

Biologically Active ◽

Membrane Phospholipids ◽

Bioactive Constituents ◽

Active Compounds ◽

Salix Purpurea ◽

Salix Myrsinifolia

Coffee and willow are known as valuable sources of biologically active phytochemicals such as chlorogenic acid, caffeine, and salicin. The aim of the study was to determine the interactions between the active compounds contained in water extracts from coffee and bark of willow (Salix purpureaandSalix myrsinifolia). Raw materials and their mixtures were characterized by multidirectional antioxidant activities; however, bioactive constituents interacted with each other. Synergism was observed for ability of inhibition of lipid peroxidation and reducing power, whereas compounds able to scavenge ABTS radical cation acted antagonistically. Additionally, phytochemicals from willow bark possessed hydrophilic character and thermostability which justifies their potential use as an ingredient in coffee beverages. Proposed mixtures may be used in the prophylaxis or treatment of some civilization diseases linked with oxidative stress. Most importantly, strong synergism observed for phytochemicals able to prevent lipids against oxidation may suggest protective effect for cell membrane phospholipids. Obtained results indicate that extracts from bark testedSalixgenotypes as an ingredient in coffee beverages can provide health promoting benefits to the consumers; however, this issue requires further study.

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Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.393 ◽

2012 ◽

Vol 506 ◽

pp. 393-396 ◽

Cited By ~ 4

Author(s):

A. Thakam ◽

N. Saewan

Keyword(s):

Ascorbic Acid ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Scavenging Activity ◽

Dpph Radical ◽

Etoac Extract ◽

Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

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Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

10.21203/rs.3.rs-930356/v1 ◽

2021 ◽

Author(s):

V. Renuka ◽

Chandragiri Nagaraja Rao Ravishankar ◽

Elavarasan Krishnamoorthy ◽

Abubacker Aliyamveetil Zynudheen

Keyword(s):

Thermal Behavior ◽

Antioxidant Activities ◽

Proteolytic Enzymes ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Structural Difference ◽

Dpph Radical ◽

Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

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Synthesis of Biologically Active Novel Indole Fused Heterocyclic Derivatives: Molecular Modeling Studies

Asian Journal of Chemistry ◽

10.14233/ajchem.2021.23179 ◽

2021 ◽

Vol 33 (7) ◽

pp. 1677-1684

Author(s):

Adamshafi Shaik ◽

Allaka Tejeswara Rao ◽

S.V.M. Mohan Rao ◽

Pilli V.V.N. Kishore

Keyword(s):

Dissociation Constants ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Biologically Active ◽

Mass Spectral ◽

E Coli ◽

Heterocyclic Derivatives ◽

C 30 ◽

Dehydrosqualene Synthase ◽

Indole Analogues

A novel series of fluoro/methoxy indole analogues 6 was synthesized and the final targets were confirmed by IR, 1H & 13C NMR and mass spectral analysis. Novel 3-substituted indole derivatives estimate for their antibacterial, antioxidant activities particularly the parent core combined with benzamides ring significantly. From antibacterial activities, compounds 6c, 6e and 6b show the highest bacterial activity against S. epidermidis, S. aureus, E. coli, with zone of inhibition 34, 30, 28 mm, respectively. Novel fluoroindole derivatives 6c, 6b, 6i shows an excellent antioxidant activity with % of inhibition 150.12, 139.04, 137.08 mmol/mL, respectively. The calculations for ligand-protein flexible of crystal structure of C(30) carotenoid dehydrosqualene synthase from S. aureus complexed with bisphosphonate BPH-700 (2ZCS). Among the designed compounds 6c exhibited highest hydrogen bonding interactions 2.06 Å, 1.85 Å with amino acids Asp27, Lys273 and binding energy -6.38 kcal/ mol, respectively. Fluoroindoles 6i, 6e and 6f shows highest ΔG = -7.90, -7.66, -7.47 kcal/mol with dissociation constants 10.32, 21.77, 22.68 μM and amino acid Lys273 interactions.

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