scholarly journals Antioxidant and Antibacterial Activities of ethanol fruit extract of Cherry Plum - Prunus cerasifera Ehrh.

2020 ◽  
Vol 10 (1-s) ◽  
pp. 45-50 ◽  
Author(s):  
K Saraswathi ◽  
C Sivaraj ◽  
P Arumugam

Foods that have disease-preventing potential are designated as functional foods. Functional foods are foods that provide health benefits beyond basic nutrition. A large number of antioxidants, both nutritive and nonnutritive, occur in foods. The potency of antioxidants present in foods in vivo will depend not only on their levels in the foods but also on their bioavailability, that is, the extent to which the active forms of antioxidants are released from the food and absorbed through the gut. Most of phytochemicals, components of food, beverages, and herbal products are often reported in literature as “nutraceutical”, emphasizing their health promoting properties, including the prevention and treatment of pathologies like cancer, cardiovascular diseases, neural disorders, and Alzheimer’s disease. Phytochemicals are virtually present in all the fruits, vegetables, pulses/legumes and grains which are commonly consumed, so it is quite easy to incorporate them in our daily diet. Current research studies were carried out for evaluating the antioxidant and antibacterial activities of ripe fruits of ethanol extract of Prunus cerasifera. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for ripe fruits of ethanol extract of Prunus cerasifera. The maximum DPPH˙ radical and Superoxide (O2.-) radical scavenging activities were 82.11±0.42% and 46.26±0.28% at 120 µg/mL concentration and the IC50 values were 45.40 µg/mL and 129.70 µg/mL concentrations respectively. The maximum ABTS●+ radical cation scavenging activity was 88.64±0.43% at 30 µg/mL concentration and the IC50 value was 10.09 µg/mL concentration respectively. The maximum Mo6+ reduction and Fe3+ reduction were 86.29±0.29% and 79.82±0.20% at 120 µg/mL concentration and the RC50 values were 20.45 µg/mL and 24.21 µg/mL concentrations respectively. The antibacterial activity of ripe fruits of ethanol extract of Prunus cerasifera showed maximum zone of inhibition of 20 mm for Bacillus subtilis at 500 µg/mL concentration.  Keywords: Antioxidant, Superoxide (O2.-) radical, ABTS●+ radical cation, Fe3+ reduction and phenolic compounds.

2019 ◽  
Vol 9 (5) ◽  
pp. 82-88
Author(s):  
K Saraswathi ◽  
C Sivaraj ◽  
B Fransila ◽  
P Arumugam

The genus Nelumbo is endowed with a number of medicinally important activities antidiabetic, antipyretic, anti-inflammatory, anti-cancerous, antimicrobial, antiviral and anti-obesity properties. Furthermore, Nelumbo nucifera flowers are served as healthy beverages to treat hypertension, cancer, diarrhea, fever, weakness, infection and body heat imbalance. It has been widely used in folk medicine for the treatment of various inflammatory and infectious diseases. Current research studies were carried out for evaluating the antioxidant, antidiabetic and antibacterial activities of fresh aqueous pink petals of Nelumbo nucifera. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for fresh aqueous pink petals of Nelumbo nucifera. The maximum DPPH˙ radical scavenging activity was 88.33±0.47% at 300 µg/mL concentration and the IC50 value was 131.68 µg/mL concentration. The maximum superoxide (O2.-) radical scavenging activity was 86.76±0.31% at 120 µg/mL concentration and the IC50 value was 21.31 µg/mL concentration. The maximum ABTS●+ radical cation scavenging activity was 69.55±0.26% at 30 µg/mL concentration and the IC50 value was 10.82 µg/mL concentration respectively. The maximum Mo6+ reduction was 84.54±0.21% at 45 µg/mL concentration and the RC50 value of Mo6+ reduction was 25.79 µg/mL concentration. The maximum Fe3+ reduction was 56.00±0.38% at 300 µg/mL concentration and the RC50 value of Fe3+ reduction was 177.30 µg/mL concentration respectively. The maximum alpha amylase enzyme inhibition was 66.37±0.10% at 300 µg/mL concentration and the IC50 value was 63.84 µg/mL concentration respectively. The antibacterial activity of fresh aqueous pink petals of Nelumbo nucifera showed maximum zone of inhibition of 14 mm for B. subtilis and minimum zone of inhibition of 10 mm for E. coli at 375 µg/mL concentration.  Keywords: Antioxidant, Superoxide (O2.-) radical, ABTS●+ radical cation, Fe3+ reduction and alpha-amylase.


Author(s):  
Intan Athirah Muhamed ◽  
Wan Amir Nizam Wan Ahmad ◽  
Nurul Syahida Ramli ◽  
Noraini A. Ghafar

Background: Curcuma longa Linn. (Zingiberaceae family) plant or turmeric is known to possess pharmacological properties at relatively nontoxic doses. The study was carried out with the aim to evaluate the antimicrobial and antioxidant activities of C. longa L.Methods: Ethanolic rhizome extract was applied against Staphylococcus aureus and Salmonella typhi using disc and well diffusion methods. Antioxidant of C. longa L. was determined by measuring 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.Results: The ethanolic extract of rhizomes showed inhibitory activity against Gram-positive S. aureus bacteria, whereas the Gram-negative S. typhi bacteria were resistant toward the ethanol extract. Zone of inhibitions against S. aureus were 7 mm and 11 mm by disc and well method, respectively. For antioxidant activity, DPPH radical scavenging activity of ethanolic extract of C. longa L. was set against butylated hydroxytoluene (BHT). It was shown that the value of half maximal inhibitory concentration (IC50) of rhizome extract was higher than standard control. The dose response curve was utilized to determine the half maximal IC50 value for each sample. The IC50 of rhizome extract is 21.25 µg/ml, while the value IC50 for BHT is 12.42 µg/ml.Conclusions: The finding suggested turmeric rhizome-extract as a good candidate in the search for a natural antimicrobial and antioxidant agent.


2020 ◽  
Vol 10 (4-s) ◽  
pp. 167-175
Author(s):  
Perumal Arumugam ◽  
Krishna Saraswathi ◽  
Dhanasekar Dhivya ◽  
Chandrasekaran Sivaraj

In recent year’s trend towards authentication, standardization and quality assurance of selected medicinal plants has been rapid increase for potential therapeutic significance. Despite the modern techniques, identification of plant drug by pharmacognostic study is more reliable. In Ayurveda, a traditional system of medicine Musa Paradisiaca is commonly used in Asthma, Diabetes, Anthelmintic, Hypertension, Insomnia, Snake bite. The whole plant as well as specific parts (leaves, ripe and unripe fruits, stems) of plant extract and its active constituents have been used for the treatment of large number of human ailments. Phytochemicals are virtually present in all the fruits, vegetables, pulses/legumes and grains which are commonly consumed, so it is quite easy to incorporate them in our daily diet. Current research studies were carried out for evaluating the antioxidant activities of ethanol bract extract of Musa paradisiaca. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, Nitric Oxide (NO) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for ethanol bract extract of Musa paradisiaca. The maximum DPPH˙ radical, Superoxide (O2.-) radical and Nitric Oxide (NO) radical scavenging activities were 52.05±0.38%, 57.71±0.45% and 49.63±0.32% at 120 µg/mL concentration and the IC50 values were 68.47 µg/mL, 71.37 µg/mL and 93.94 µg/mL concentrations respectively. The maximum ABTS●+ radical cation scavenging activity was 84.28±0.13% at 30 µg/mL concentration and the IC50 value was 10.04 µg/mL concentration respectively. The maximum Mo6+ reduction and Fe3+ reduction were 45.38±0.40% and 57.06±0.32% at 120 µg/mL concentration and the RC50 values were 121.44 µg/mL and 85.43 µg/mL concentrations respectively. Z-2-Tridecen-1-ol, Nonadecane-2,4-dione, a-Ketostearic acid, 2,5-Furandione, dihydro-3-(2-tetradecenyl)- were found to be the potent antioxidant compounds detected from GC-MS analysis. Keywords: Antioxidant, phenolic compounds, Nitric Oxide (NO) radical, ABTS●+ radical cation, Fe3+ reduction and a-Ketostearic acid.


2018 ◽  
Vol 7 (1) ◽  
pp. 72-78
Author(s):  
Vardhini SP ◽  
◽  
Sivaraj C ◽  
Arumugam P ◽  
Himanshu Ranjan ◽  
...  

Aegle marmelos (Bael) is an important medicinal plant which belongs to family Rutaceae. Leaves, fruit, stem, bark of this plant is used because of its medicinal properties like astringent, antidiarrheal, antipyretic, antiinflammatory activities. Compounds purified from A.marmelos have been proven biologically active against various several major diseases like cancer, diabetes, cardiovascular diseases. The aim of the present study was to evaluate the antioxidant, anticancer and antibacterial activities of aqueous extract of A. marmelos and to identify the bioactive compounds by GC-MS analysis. Antioxidant activities such as DPPH radical, ABTS·+ radical cation, Fe3+ reducing power and phosphomolybdenum reduction assays were carried out for aqueous extract. The maximum DPPH radical and ABTS·+ radical cation scavenging activities were 60.70±4.24% at 300 µg/mL and 95.77±6.70% at 30 µg/mL concentrations. The IC50 values of DPPH˙ radical and ABTS·+ radical cation scavenging activities were 183.58 µg/mL and 10.81 µg/mL concentration. The maximum reduction of Fe3+ and Mo6+ were 52.05±3.64% and 87.81±6.19% at 120 µg/mL concentration and the IC50 values were 102.83 µg/mL and 32.76 µg/mL concentration. The anticancer activity was carried out by MTT assay method, which showed the IC50 value of 47.92 µg/mL concentration


2019 ◽  
Author(s):  
Chem Int

In this study, we determined the chemical composition and antioxidant activities of the essential oils from two different varieties of khat (Catha edulis Forsk) cultivated in Ethiopia. The essential oils were extracted by hydrodistillation using the Clevenger type apparatus, identifications of compounds were made by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Seventy seven different compounds were identified from essential oils of the two different khat cultivars. The essential oils in the samples from Bahir Dar and Wendo were composed of 50 and 34 compounds, respectively. The major compound identified in khat essentials oils include: limonene, 1-phenyl-1,2-propanedione, 1-hydroxy,1-phenyl-2-propanone, camphor, (sulfurous acid)-2-propylundecyl ester, hexadecane, O-mentha-1(7), 8-dien-3-ol, heptadecane, 10-methylnonadecane, (phthalic acid)-isobutyl octadecyl ester, and tritetracontane. The antioxidant and free radical scavenging activity of the oils were assessed by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activities of the oils were 23.5-23.6 μg AAE/kg of fresh khat sample.


Author(s):  
Atefeh Jalali ◽  
Mohammadreza Kiafar ◽  
Masih Seddigh ◽  
Mohammad M. Zarshenas

Background: The consumption of natural antioxidants is increasing due to the demand and tendency to natural foods. Punica granatum L. [Punicaceae] is a fruit with various bioactive ingredients. The effectiveness of this plant has been proved against various disorders such as hyperglycemia, hyperlipidemia, blood coagulation, infections, cancer, and dentistry. Among them, there are numerous researches on antimicrobial and antioxidant properties. Subsequently, the present study aimed to compile a review of those properties to outline this herb as a possible natural antioxidant and preservative. Methods: Synchronically, keywords "Punica granatum" with antimicrobial, or antibacterial, antifungal, antiviral, antioxidant and radical scavenging were searched through "Scopus" database up to 31st September 2019. Papers focusing on agriculture, genetics, chemistry, and environmental sciences were excluded and also related papers were collected. Results: Among 201 papers focusing on related activities, 111 papers have dealt with antioxidant activities focusing based on DPPH assay, 59 with antibacterial, on both gram+ and gram- bacteria, 24 with antifungal effects, mostly on Aspergillus niger and Candida albicans, and 7 papers with antiviral activities. There were about 50 papers focusing on in-vivo antioxidant activities of this plant. Conclusion: Taken together, botanical parts of P. granatum have possessed notable radical scavenging and antimicrobial activities that with these properties, this plant can be introduced as a natural safe source of preservative and antioxidant. Accordingly, P. granatum can be applied as excipient with the aforementioned properties in the pharmaceutical and food industries.


Author(s):  
Ganiyu Oboh ◽  
Veronica O. Odubanjo ◽  
Fatai Bello ◽  
Ayokunle O. Ademosun ◽  
Sunday I. Oyeleye ◽  
...  

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.


2021 ◽  
Author(s):  
E.K. Soltani ◽  
K. Zaim ◽  
K. Mokhnache ◽  
N. Haichour ◽  
S. Mezaache-Aichour ◽  
...  

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.


2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.


2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.


Sign in / Sign up

Export Citation Format

Share Document