scholarly journals ISOLATION, IDENTIFICATION AND SENSITIVITY OF AMILOLITIC BACTERIA FROM MANGROVE ECOSYSTEM SEDIMENT IN PURNAMA MARINE STATION DUMAI ON THE PATHOGENIC BACTERIA

2020 ◽  
Vol 2 (3) ◽  
pp. 257-266
Author(s):  
Lamtiur Rotua Silitonga ◽  
Nursyirwani Nursyirwani ◽  
Irwan Effendi
Keyword(s):  
16S Rrna ◽  
Pathogenic Bacteria ◽  
Dna Analysis ◽  
Bacterial Species ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
16S Rrna Sequence ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Marine Station

Litter from the weathering of dead mangrove stems and leaves contains a lot of starch which has potential to be degraded by amylolytic bacteria into simple compounds with the help of the amylase enzyme. Amylolytic bacteria are bacteria that hydrolyze starch into simpler compounds namely glucose with the help of the amylase enzyme. This study aims to 1) isolate, identify and test sensitivity of amylolytic bacterial isolates found at the Purnama Dumai Marine Station, 2) the ability of amylolytic bacterial isolates to inhibit the growth of pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Vibrio alginolyticus) and 3) to determine the of amylolytic bacterial species by 16S rRNA sequence analysis. The results showed 10 bacterial isolates (TR 2, TR 6, TR 7, TR 9, TR 11, TR 13, TR 15, TR 16, TR 18 and TR 20) were able to inhibit the growth of pathogenic bacteria (E.coli, P.aeruginosa and V.alginolyticus). The sensitivity test of isolate TR 20 against E.coli was categorized into weak with inhibition zone diameter of 4.65 mm. Sensitivity of isolate TR 6 against P.aeruginosa was categorized into medium with inhibition zone diameter of 5.22 mm. Then sensitivity of isolate TR 11 against V.algynolyticus was categorized into medium with inhibition zone diameter of 5.55 mm. DNA analysis using 16S rRNA method and BLAST analysis showed similarity of each isolate. Isolate TR 6 was similar to Bacillus paramycoides strain MCCC 1A04098, isolate TR 11 was in a group of Enterobacter cloacae strain ATCC 13047 and TR 20 was in a group of Vibrio harveyi strains of NBRC 15634.

scholarly journals Use of metabolites of therapeutic Lactic acid bacteria to inhibit four species veterinary pathogenic bacteria

2005 ◽  
Vol 29 (2) ◽  
pp. 49-55
Author(s):  
Ali H. A . Hamid
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Inhibition Zone ◽  
Culture Cell ◽  
Inhibition Activity ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Mrs Broth

This study aimed to detect metabolites inhibition activity for three therapeuticLactic acid bacteria Lactobacillus acidophilus (Lb. acid.) , Lactobacillus reuteri(Lb. reut.) and Bifidobacterium ssp. (Bif.) which were grown in whole milk andMan Reqosa and Sharp (MRS) broth as single or double (Lb. acid. + Bif.) ormixed (Lb. reut + Bif. + Lb. acid) culture Cell Free Extract (CFE) of theseculture which contained their metabolites of Lactic acid bacteria (exclusion Lb.reut.) showed good inhibition against four species of pathogenic Escherichiacoli ; Salmonella typhimurium ; Proteus volgaris and Staphylococcus aureus.inhibition activity against E. coli culture showed that 72 hours was the bestincubation time for Lactic acid bacteria to produce more metabolite with bestinhibition activity. Metabolite (CFE) of Lb. Acid culture grown in milk andMRS broth showed the best inhibition activity against all of Gram Positive andGram negative test bacteria with over all inhibition zone diameter was 14.6 and15.3 mm for milk and MRS broth supernatant respectively and this activityremained for more than one week as inhibition zone diameter were 13.4 and14.5 mm for supernatant of milk and MRS respectively.

scholarly journals Evaluation of Toxicity and Antimicrobial Activity of Watery and Alcoholic extracts of some plant on growth of pathogenicBacteria isolatedfrom diarrhea

2012 ◽  
Vol 36 (1) ◽  
pp. 25-32
Author(s):  
Hanan Adnan Shaker Al- Naemi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Bacterial Species ◽  
Inhibition Zone ◽  
Thymus Vulgaris ◽  
Acidic Ph ◽  
Bacterial Isolates ◽  
Active Components ◽  
Biochemical Tests ◽  
Inhibition Zone Diameter

In This study, the bacteria Escherichia Coli, Salmonella typhimurium and Shigella dysentarea were isolated from diarrhea in infants. Microbiological and biochemical tests were conducted to identify these bacteria. In the Identification of bacterial Species the API-System was used. Sensitivity test of bacterial isolates revealed high resistance to many Antibiolics like Ampicillin, Tetracycline, Ceftazidine, Cephalothin ,Vancomycin and Rifamipicin.The effects of extracts of (Citrus aurantifolia ,Zingiber offienals, Glycyrrhiza glabra, Pimpinellaanisum, Camellia ,sinessis Coffeiaarabica, Elattariacar domomum, Cumminum cumminum, Thymus vulgaris and Trigonella foenum geoecum in inhibiting bacteria isolated from diarrhea were studied , also indication of presence of chemical active components in extracts was observed . The Preliminary chemical tests revealed acidic PH of all extracts, and the best antibacterial was effect that of Citrus aurantifolie an lcoholic extract on growth of E.coli with inhibition zone diameter 47mm.

scholarly journals Enterobacterial Repetitive Intergenic Consensus (ERIC) as a tool for genetic characterisation of bacterial isolates in Nigeria

2020 ◽  
Vol 37 (1) ◽  
pp. 122-128
Author(s):  
K. Otokunefor ◽  
C.J. Ogugbue ◽  
B.U. Fajoyomi
Keyword(s):  
16S Rrna ◽  
Bacterial Species ◽  
Pcr Analysis ◽  
Bacterial Identification ◽  
Bacterial Isolates ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
16S Rrna Sequence ◽  
Genetic Characterisation ◽  
Polymerase Chain ◽  
Identification Tool

Genetic characterisation as a tool for identification of bacterial isolates in Nigeria has been on the increase in recent years, and the 16s rRNA typing has been a preferred method. Due to cost limitations, there is a need to explore other genetic options. Enterobacterial Repetitive Intergenic Consensus (ERIC) polymerase chain reaction (PCR) analysis is a PCR- only based system which offers the advantage of reduced cost. This study set out to explore the use of ERIC-PCR in genetic characterisation of some selected bacterial isolates from Nigeria and compare it with genetic characterisation using 16s rRNA sequence typing. ERIC-PCR and 16s rRNA typing were carried out on 15 isolates following previously described protocols. Using 16s rRNA typing, thirteen different bacterial species were identified of which majority (85.7%) were Gram negative, with 57.1% belonging to the Enterobacteriaceae family. Using ERIC-PCR, only 13 of the 15 isolates (86.7%) could be typed, resulting in the identification of the 13 different types. ERIC-PCR was able to accurately differentiate between two members of the Proteus species, as well as identify the organisms as similar based on the banding pattern. The results show that ERIC-PCR may play a role as a bacterial identification tool but this role might be more suited to differentiating closely related members of a genus or typing within species rather than general bacterial identification. Keywords: Genetic characterisation, 16s rRNA, ERIC-PCR, Nigeria

scholarly journals Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)

2018 ◽  
Vol 26 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Hansani N.K.S. Pathirana ◽  
Sudu H.M.P. Wimalasena ◽  
Benthotage C.J. De Silva ◽  
Sabrina Hossain ◽  
Gang-Joon Heo
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Paralichthys Olivaceus ◽  
Inhibition Zone ◽  
Olive Flounder ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
Citrus Aurantifolia ◽  
Zone Diameter

Abstract The antibacterial activity of lime (Citrus aurantifolia) essential oil (LEO) and limonene was tested against seven Gram-negative and nine Gram-positive fish pathogenic bacteria isolated from cultured olive flounder, Paralichthys olivaceus (Temminck & Schlegel) in Korea. Limonene was >99% concentrated and LEO consisted of eleven chemical compounds including 56.22% of limonene. Disk diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests were done. LEO and limonene inhibited the growth of both Gram-negative and Gram-positive bacteria. LEO and limonene (MBC/MIC= 2-8) were both bactericidal and bacteriostatic for the strains tested. In every fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100% (V/V) concentrations of LEO and limonene. The antibiogram pattern indicated that all the bacterial strains, excluding three strains of S. iniae (S186, S530, and S131), showed resistance to one or more antibiotics. The percentage of the relative inhibition zone diameter (RIZD %) exhibited high values at higher concentrations of all the agents. Since antibacterial activities of LEO and limonene were considerably effective against fish pathogenic bacteria, they could be used as alternatives to treat bacterial infections in aquaculture.

scholarly journals Antibacterial activity of cinnamon (Cinnamomum zeylanicum) essential oil and cinnamaldehyde against fish pathogenic bacteria isolated from cultured olive flounder Paralichthys olivaceus

2019 ◽  
Vol 66 (2) ◽  
Author(s):  
H.N.K.S. Pathirana ◽  
S.H.M.P. Wimalasena ◽  
B.C.J. De Silva ◽  
S. Hossain ◽  
G.J. Heo
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Paralichthys Olivaceus ◽  
Inhibition Zone ◽  
Cinnamomum Zeylanicum ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Olive Flounder Paralichthys Olivaceus

Antibacterial activity of cinnamon (Cinnamomum zeylanicum) essential oil (CEO) and cinnamaldehyde was tested against seven Gram negative and nine Gram positive fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus) in Korea. Disk diffusion assay, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) tests were done. Cinnamon and cinnamaldehyde inhibited the growth of both Gram negative and Gram positive bacteria. Cinnamaldehyde showed lower MIC values than CEO which ranged from 0.001 to 0.015% (V/V). CEO (MBC/MIC 2-4) was bactericidal for all tested strains while cinnamaldehyde was bactericidal for all except 6 strains. For all fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100% (V/V) concentration of CEO and cinnamaldehyde.  The antibiogram pattern indicated that all the bacterial strains excluding three strains of Streptococcus iniae(S186, S530 and S131) showed resistance to one or more antibiotics. Higher percentages of relative inhibition zone diameter (RIZD %) were exhibited at higher concentrations of CEO and cinnamaldehyde. Since antibacterial activities of CEO and cinnamaldehyde were considerably effective against fish pathogenic bacteria, these could be used as alternatives to treat bacterial infections in aquaculture.

scholarly journals Antifungal and growths regulation activity of new metal-organic compounds

2019 ◽  
Vol 16 (2) ◽  
pp. 143-149
Author(s):  
O. V. Bashta ◽  
L. P. Pasichnyk ◽  
N. M. Voloshchuk ◽  
G. G. Repich ◽  
O. O. Zholob ◽  
...  
Keyword(s):  
Seed Germination ◽  
Organic Compounds ◽  
Copper Complex ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chelate Complexes ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Grain Crops ◽  
Metal Organic

The aim of present paper is to study the antifungal activity of the new platinum, palladium and copper chelate complexes with different organic ligands against Fusarium strains and to determine the influence of these compounds on the germination energy and seed germination of investigated grain crops. Methods. In vitro antifungal activities of new chelate complexes were studied by the agar disk diffusion method. New complexes were tested against pathogenic Fusarium strains such as — F. culmorum 3260/4, F. verticillioides and F. sporotrichioides. The toxic effect of new complexes was evaluated according to changes in germination power and seed germination of grain crops — winter wheat, vernal barley and maize. The determination of these characteristics was carried out in accordance with the standard demands. Results. Our results revealed that palladium complex H exhibited fungicidal activity against F. culmorum 3260/4 and fungistatic activity against F. verticillioides with growth inhibition zone diameter 25 mm ad 20 mm respectively. Copper complex 2 showed a moderate fungicidal effect against F. culmorum 3260/4 strain (inhibition zone diameter 12 mm). The treatment of grain crops seeds with the H and 2 complexes demonstrated the less toxicity than standard agent — commercial fungicide tebuconazol. These complexes had also less negative influence on the germination power for all test cultures than tebuconazol. Copper complex 2 showed a noticeable growth promoting effect on studied seeds compared to seeds under conditions without treatment. Conclusions. Thus, the studied complexes can be considered as promising antifungal agents with growth regulating properties. The directional modification of the complexes to obtain the more potent derivatives will be performed.Keywords: metal-organic compounds, antifungal, growths regulation activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals Bioactivity of Selected Phenolic Acids and Hexane Extracts from Bougainvilla spectabilis and Citharexylum spinosum on the Growth of Pectobacterium carotovorum and Dickeya solani Bacteria: An Opportunity to Save the Environment

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 482 ◽  
Author(s):  
Nader A. Ashmawy ◽  
Said I. Behiry ◽  
Asma A. Al-Huqail ◽  
Hayssam M. Ali ◽  
Mohamed Z. M. Salem
Keyword(s):  
Phenolic Acids ◽  
Tannic Acid ◽  
Defense Mechanisms ◽  
Pathogenic Bacteria ◽  
Soft Rot ◽  
Inhibition Zone ◽  
Pectobacterium Carotovorum ◽  
Bacterial Isolates ◽  
Dickeya Solani

Phenolic acids and natural extracts, as ecofriendly environmental agents, can be used as bio bactericides against the growth of plant pathogenic bacteria. In this study, isolation trails from infected potato tubers and stems that showed soft rot symptoms in fields revealed two soft rot bacterial isolates and were initially identified through morphological, physiological, and pathogenicity tests. The molecular characterization of these isolates via PCR, based on the 16S rRNA region, was carried out by an analysis of the DNA sequence via BLAST and Genbank, and showed that the soft rot bacterial isolates belong to Pectobacterium carotovorum subsp. carotovorum (PCC1) and Dickeya solani (Ds1). The in vitro results of the tested phenolic acids against the cultured bacterial isolates proved that concentrations of 800, 1600, and 3200 μg/mL were the most effective. Ferulic acid was the potent suppressive phenolic acid tested against the Ds1 isolate, with an inhibition zone ranging from 6.00 to 25.75 mm at different concentrations (25–3200 μg/mL), but had no effect until reaching a concentration of 100 μg/mL in the PCC1 isolate, followed by tannic acid, which ranged from 7.00 to 25.50 mm. On the other hand, tannic acid resulted in a significant decrease in the growth rate of the PCC1 isolate with a mean of 9.11 mm. Chlorogenic acid was not as effective as the rest of the phenolic acids compared with the control. The n-hexane oily extract (HeOE) from Bougainvillea spectabilis bark showed the highest activity against PCC1 and Ds1, with inhibition zone values of 12 and 12.33 mm, respectively, at a concentration of 4000 μg/mL; while the HeOE from Citharexylum spinosum wood showed less activity. In the GC/MS analysis, nonanal, an oily liquid compound, was found ata percentage of 38.28%, followed by cis-2-nonenal (9.75%), which are the main compounds in B. spectabilis bark HeOE, and 2-undecenal (22.39%), trans-2-decenal (18.74%), and oleic acid (10.85%) were found, which are the main compounds in C. spinosum wood HeOE. In conclusion, the phenolic acids and plant HeOEs seem to raise the resistance of potato plants, improving their defense mechanisms against soft rot bacterial pathogens.

Sign in / Sign up

Export Citation Format

Share Document