scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

scholarly journals DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2015 ◽  
Vol 17 (1) ◽  
pp. 149
Author(s):  
Fahri Bian ◽  
Febby E.F. Kandou ◽  
Marhaenus J. Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta  terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan  29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF LIME JUICE EXTRACT AGAINST ESCHERICHIA COLI

2020 ◽  
Vol 3 (1) ◽  
pp. 135-139
Author(s):  
Nerdy Nerdy ◽  
Palas Tarigan ◽  
Elysa Elysa ◽  
Puji Lestari ◽  
Siti Nurmalisa
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Lime Juice ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Escherichia Coli Bacteria

Lime (Citrus aurantifolia Swingle) is known to have seceral phytochemical contents (flavonoids and saponins) that can be memiliki berbagai aktivitas farmakologis yang bermanfaat. Salah satu aktivitas farmaokologis yang diberikan oleh flavonoid adalah sebagai antibacterial activity. The aims of this research was to determine the antibacterial activity of lime juice against Escherichia coli bacteria. This research used an experimental laboratory research method using antibacterial effectiveness trials against the growth of Escherichia coli bacteria. The research design for antibacterial effectiveness used the lime fruit extract method with 4 treatment groups, positive control and negative control. Antibacterial effectiveness was observed the inhibition zone diameter by disc diffusion method. Results of the Escherichia coli antibacterial effectiveness test obtained at a concentration of 100% had antibacterial activity with an average diameter of the inhibition zone 19.9 mm. The lowest concentration that still provides antibacterial resistance against Escherichia coli is at a concentration of 12.5% ​​with an average diameter of the inhibition zone of 17.9 mm. The results obtained indicated that the antibacterial effectiveness test on lime fruit extract is very strong. At a concentration of 100% it has the best antibacterial activity, the inhibition zone diameter similar with the inhibition zone of 500 mg tetracycline.

scholarly journals Uji Efek Antibakteri Chromodoris annae terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Aqueline N. Faidiban ◽  
Jimmy Posangi ◽  
Pemsi M. Wowor ◽  
Robert A. Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Escherichia Coli Bacteria

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris annae taken from Bunaken waters. This was an experimental study. The antibacterial effect was tested by using the Kirby-Bauer method. Chromodoris annae extract was made by maceration using 95% ethanol and was tested to Staphylococcus aureus and Escherichia coli bacteria. Ciprofloxacin was used as the positive control and aquadest as the negative control. The results showed that the mean inhibition zone diameter of Chromodoris annae extract to Staphylococcus aureus was 22.3 mm meanwhile of ciprofloxacin was 34.7 mm. The mean inhibition zone diameter of Chromodoris annae extract to Escherichia coli was 23.0 mm meanwhile of ciprofloxacin was 40.3 mm. Moreover, aquadest showed no inhibition zone. In conclusion, Chromodoris annae had very strong antibacterial effect to the growths of Staphylococcus aureus and Escherichia coli.Keywords: Chromodoris annae, Staphlococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk mengevaluasi efek antibakteri dari Chromodoris annae yang diambil dari perairan Bunaken. Jenis penelitian ialah eksperimental. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer. Ekstrak Chromodoris annae dibuat dengan cara maserasi menggunakan pelarut etanol 95% dan diujikan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sebagai kontrol positif digunakan antibiotik ciprofloxacin dan sebagai kontrol negatif digunakan akuades. Hasil penelitian memperlihatkan rerata zona hambat ekstrak Chromodoris annae terhadap Staphylococcus aureus sebesar 22,3 mm sedangkan zona hambat ciprofloxacin sebesar 34,7 mm. Rerata zona hambat ekstrak Chromodoris annae terhadap Escherichia coli sebesar 23,0 mm sedangkan zona hambat ciprofloxacin sebesar 40,3 mm. Akuades tidak memperlihatkan adanya zona hambat. Simpulan penelitian ini ialah Chromodoris annae memiliki efek antibakteri kategori sangat kuat terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris annae, Staphylococcus aureus, Escherichia coli

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Nurkayah Nurkayah ◽  
Elisa Nurnawati ◽  
Hary Widjajanti
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Trichoderma Harzianum ◽  
Inhibitory Concentration ◽  
Pathogenic Fungus ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Colletotrichum Capsici ◽  
Layer Chromatography

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.

scholarly journals UJI RESISTENSI BAKTERI ESCHERICHIA COLI YANG DIISOLASI DARI PLAK GIGI TERHADAP MERKURI DAN ANTIBIOTIK KLORAMFENIKOL

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Ronal Dian ◽  
Fatimawali . ◽  
Fona Budiarso
Keyword(s):  
Escherichia Coli ◽  
Dental Plaque ◽  
Bacterial Infections ◽  
Anaerobic Bacteria ◽  
Inhibition Zone ◽  
Chemical Substance ◽  
Luria Bertani ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Dental Filling

Abstract: Mercury is a liquid element at room temperature. Some main effects of mercury are dysfunctional of brain, kidneys and lungs, tremor, anxiety or nervous, insomnia, fetal growth restriction, and liver damage in pregnancy. Dental plaque is a soft material that attach on tooth’s surface, dental filling, or calculus. Antibiotic is a chemical substance that has the ability to inhibit the growth and kill microorganisms. Chloramphenicol is a broad-spectrum antibiotic that useful for treatment of a number of bacterial infections and anaerobic bacteria. The research using descriptive method. Subject of the research was mercury resistence Escherichia coliwhich isolated from dental plaque with dental filling and grew at broth Luria Bertani (LB) and solid LB. The result of observation on bacteria after incubated at 370C for 24 hours showed that at HgCl2, with available dilution, found bacteria at dental plaque with higher amounts of growth at 10ρρm concentration, intermediate amount of growth at 20ρρm concentration, and less amount of growth at 40ρρm concentration. As the result of observation on Escherichia coli after 24 hours of incubation, researcher found that the inhibition zone diameter of Eschericia coli for 30μg chloramphenicol was 20mm (>12mm) during first, second, and third treatment and included in sensitive category. Conclusion: As the result of this research, the inhibition zone diameter of Eschericia coli for 30μg chloramphenicol was 20mm (>12mm) during first, second, and third treatments and included in sensitive category.Keywords: mercury, dental plaque, chloramphenicol, Eschericia coli.Abstrak: Merkuri adalah suatu unsur berbentuk cair keparatan dalam suhu kamar. Resiko utama merkuri adalah tidak berfungsinya otak, ginjal, tremor, rasa gelisah atau gugup, insomnia, gangguan fungsi paru-paru, memperlambat pertumbuhan janin, kerusakan liver pada kehamilan. Plak gigi adalah deposit lunak yang melekat pada permukaan gigi, tumpatan, maupun kalkulus. Antibiotik adalah zat kimia yang mempunyai kemampuan untuk menghambat pertumbuhan dan membunuh mikroorganisme. Kloramfenikol adalah antibiotik spektrum luas yang efektif terhadap beberapa jenis bakteri dan bakteri anaerob. Penelitian adalah metode deskriptif eksploratif. Sampel yang diambil dalam penelitian adalah bakteri Escherichia coli resistensi merkuri yang diisolasi dari plak gigi dengan tumpatan amalgam dan tumbuh pada media Luria Bertani (LB) broth dan media LB padat.Pengamatan setelah bakteri diinkubasi pada suhu 370C selama 24 jam didapatkan hasil bahwa pada HgCl2 dengan pengeceran yang ada, di dapatkan bakteri isolat yang diidentifikasi dari plak gigi dengan hasil pertumbuhan yang banyak terdapat pada konsentrasi 10ρρm, pertumbuhan sedang terdapat pada konsentrasi 20ρρm, dan pertumbuhan sedikit terdapat pada konsentrasi 40ρρm. Pengamatan setelah bakteri Escherichia coli diinkubasi selama 24 jam didapatkan bahwa diameter zona hambat Escherichia coli terhadap antibiotik kloramfenikol 30μg pada pengulangan pemberian I, II, dan III sebesar 20mm (>12mm) dan termasuk dalam kategori sensitif. Simpulan: Hasil uji yang tekah dilakukan didapatkan bahwa diameter zona hambat Escherichia coli terhadap antibiotik kloramfenikol 30μg pada pemberian I,II,dan III sebesar 20mm (>12mm) termasuk dalam kategori sensitif.Kata kunci: merkuri, plak gigi, kloramfenikol, bakteri Echerichia coli

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

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