scholarly journals Identifikasi Genetik Mutan Talinum paniculatum JACQ. (GAERTN.) Berdasarkan Marka RAPD

2012 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
Yuyu Suryasari Poerba
Keyword(s):  
Clustering Analysis ◽  
Room Temperature ◽  
Genetic Variance ◽  
Plant Root ◽  
Ethyl Methane Sulphonate ◽  
The Third ◽  
Induce Mutation ◽  
Methane Sulphonate ◽  
Putative Mutant

Javasom Talinum paniculatum Jacq. (Gaertn.) is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetableand as a substitute for purslane (Portulaca olaraeae L.). An effort to improve genetic quality of the plant was madethrough induced mutation with ethyl methane sulphonate (EMS). A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%,1.2%, 1.5% dan 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. Selectedfive putative mutant genotypes of the third generation after mutagen treatment (M3) was analyzed using Ramdomamplified polymorphic DNA (RAD) marker. Twenty RAPD primers generated 185 scorable bands with 78.92% ofthem were polymorphic. Size of the bands varied from 200bp to 2.5kbp. Clustering analysis was performed basedon RAPD profiles using the UPGMA method. The range of genetic distance among individual genotypes was fromfrom 0.31 to 0.58, while genetic variance was 0.29 + 0.17. The five genotypes were proof to be solid mutants.

scholarly journals INDUKSI MUTASI KULTUR IN VITRO Amorphophallus muelleri Blume DENGAN IRRADIASI GAMMA

2016 ◽  
Vol 10 (3) ◽  
pp. 355
Author(s):  
Yuyu S Poerba ◽  
MARIA IMELDA ◽  
AIDA WULANSARI ◽  
DIYAH MARTANTI
Keyword(s):  
Gamma Irradiation ◽  
Gamma Rays ◽  
Clustering Analysis ◽  
Genetic Variance ◽  
Paper Industry ◽  
Conservation Strategy ◽  
Induce Mutation ◽  
Polymorphic Bands

Amorphophallus muelleri Blume (Araceae) is valued for its glucoman content for use infood industry (heathy diet food), paper industry, pharmacy and cosmetics. The cultivationof A. muelleri is hampered by limited genetic quality of seed. The species is triploid(2n=3x=39), the seed is developed apomictically, and pollen production is low. Thespecies is only propagated vegetatively. This may explain that the species is difficultto breed conventionally and genetic variability in the existing landraces cultivars israther limited. Conservation of this species, therefore, is important for availability of thespecies in the future use of this plant. The objective of present research is to increasegenetic variation by induce mutation using gamma-rays irradiation of shoot culturesof A. muelleri and to identify DNA polymorphism induced by gamma irradiation usingrandom amplified polymorphic DNA (RAPD), so the mutants produced can be used forbreeding purposes and for conservation program. Results of the experiment showedthat gamma irradiation less than 5 gray was effective to induce mutation of A. muelleri.Four RAPD primers generated 35 scorable bands with 100% polymorphic bands. Sizeof the bands varied from 350bp to 2.0kbp. Clustering analysis was performed based onRAPD profiles using the UPGMA method. The range of genetic distance among individualgenotypes was from from 0.00 to 0.72, while genetic variance of the population was0.21 + 0.13. The eighteen genotypes were proof to be mutants. The mutants producedin this experiment could be used as new germplasms for breeding purposes as well asfor use in conservation strategy

The prediction of the cyclic mechanical behavior of stainless steel 304L at room temperature

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Hala Messai ◽  
Salim Meziani ◽  
Athmane Fouathia
Keyword(s):  
Stainless Steel ◽  
Design Methodology ◽  
Room Temperature ◽  
Type Model ◽  
Good Prediction ◽  
304L Stainless Steel ◽  
Content Type ◽  
The Third ◽  
Chaboche Model

Purpose The purpose of this paper is to highlight the performance of the Chaboche model in relation to the database identification, tests with imposed deformations were conducted at room temperature on 304L stainless steel specimens. Design/methodology/approach The first two tests were performed in tension-compression between ±0.005 and ±0.01; in the third test, each cycle is composed of the combination of a compression tensile cycle between ±0.01 followed by a torsion cycle between ±0.01723 (non-proportional path), and the last, uniaxial ratcheting test with a mean stress between 250 MPa and −150 MPa. Several identifications of a Chaboche-type model were then performed by considering databases composed of one or more of the cited tests. On the basis of these identifications, the simulations of a large number of ratchet tests in particular were carried out. Findings The results present the effect of the optimized parameters on the prediction of the behavior of materials which is reported in the graphs, Optimizations 1 and 2 of first and second tests and Optimization 4 of the third test giving a good prediction of the increasing/decreasing pre-deformation amplitude. Originality/value The quality of the model's predictions strongly depends on the richness of the database used for the identification of the parameters.

scholarly journals PENGARUH PENGEMASAN VAKUM TERHADAP KUALITAS BANDENG PRESTO SELAMA PENYIMPANAN

2018 ◽  
Vol 11 (2) ◽  
pp. 63
Author(s):  
Lukita Purnamayati ◽  
Ima Wijayanti ◽  
Apri Dwi Anggo ◽  
Ulfah Amalia ◽  
Sumardianto Sumardianto
Keyword(s):  
Fatty Acids ◽  
High Pressure ◽  
Free Fatty Acids ◽  
Shelf Life ◽  
Room Temperature ◽  
Vacuum Packaging ◽  
The Third ◽  
Fishery Product

Softbone milkfish is a fishery product that easily damaged during storage. One of the efforts to inhibit the<br />damage of softbone milkfish was using vacuum packaging. The purpose of this study was to determine the<br />changes quality of softbone milkfish which were vacuum packed during storage at room temperature. Fresh<br />milkfish was heating with high pressure by using a pressure cooker. Softbone milkfish was then stored at room<br />temperature for six days. Analysis was carried out every three days for the parameters of free fatty acids, TVBN,<br />and organoleptics. The results showed that the number of free fatty acids and TVBN increased during storage.<br />Free fatty acids and TVBN of softbone milkfish with vacuum packaging after sixth days of storage were 2.95%<br />and 15.95 mgN/100g, respectively. This value was lower than the non-vacuum softbone milkfish. Based on<br />organoleptic analysis, softbone milkfish with vacuum packaging was still suitable for consumption until the third<br />day of storage. This showed that vacuum packaging can extend the shelf life of softbone milkfish.

scholarly journals Changes in quality of natural orange juice pasteurised by high pressure during storage

2000 ◽  
Vol 18 (No. 5) ◽  
pp. 187-193 ◽  
Author(s):  
J. Strohalm ◽  
H. Valentová ◽  
M. Houška ◽  
P. Novotná ◽  
A. Landfeld ◽  
...  
Keyword(s):  
High Pressure ◽  
Sensory Evaluation ◽  
Orange Juice ◽  
Room Temperature ◽  
Preference Test ◽  
Quality Parameters ◽  
The Third ◽  
Heat Treated ◽  
Acceptable Quality

Natural orange juice pasteurised (pascalised) by high pressure was stored for 180 days at room temperature in dark conditions. Sensory evaluation of several quality parameters was made. At the same time the frozen and heat pasteurised parallel samples of the juice of the same origin were evaluated for comparison. The sensory evaluation consists of overall image, flavour, taste and sensorial viscosity. The instrumental evaluation of colour, pH and kinematic viscosity was made. The best overall image was received for frozen juice. The pressurised and heated samples were nearly the same as regards the flavour. The sensorial viscosity of all samples was evaluated as thin with very small differences during storage. The preference test was also made – the best quality for the test panel was received for samples of frozen juice followed by pressurised juice. The third rank was given to heat treated samples. The pressurised samples exhibited the acceptable quality for 150 days of storage at room temperature.

scholarly journals Aktivitas Peroksidase Mutan Pisang Kepok dengan Ethyl Methane Sulphonate (EMS) secara In Vitro 1

2012 ◽  
Vol 14 (1) ◽  
pp. 32
Author(s):  
Yulmira Yanti
Keyword(s):  
Leaf Tissue ◽  
Band Pattern ◽  
Ethyl Methane Sulphonate ◽  
Migration Distance ◽  
Ethyl Methane ◽  
The Third ◽  
Methane Sulphonate ◽  
And Control ◽  
Relative Migration

The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments EMS. The phenotype of peroxidase wasanalyzed from the banana clones resulting the induced by EMS and without induction after inoculated with pathogen Banana Blood deasesBacterium (BBD). The objectives of research are know variation of activity and band pattern of peroxidase. Induced mutation treatmentsconsist of control, 0.2% EMS for 1 and 3 hours, 0.5% for 1 and 3 hours, each treatments was provided five banana clones. The result showedthat variant value and coefisien variant of peroxidase activity in leaf tissue of clone treated by EMS increased compare to the control. Avariant control is 0.28 with coefisien variant is 29.92%, while variant value of treatment is 8.45 with coefisien variant is 75.75%.Appearance of peroxidase bands on clone resulting by EMS treatments were emerged four band pattern. The first and control band patternhas relative migration distance is 20 and 30, the second 15, 40 and 60, the third is 15, 35 and 50. There are four bands with different relativemigration distance that indicated polymorphic.

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

THE STUDY ON TA VAT WINE PRODUCTION FROM SUGAR PALM (ARENGA PINNATA) SAP

2014 ◽  
Vol 83 (5) ◽  
Author(s):  
Nguyễn Thị Hồng Thu ◽  
Đặng Minh Nhật ◽  
Nguyễn Hoàng Dung
Keyword(s):  
Room Temperature ◽  
Fermentation Process ◽  
Inoculum Size ◽  
Wine Fermentation ◽  
Wine Production ◽  
Factors Affecting ◽  
Natural Fermentation ◽  
Tropical Asia ◽  
Best Parameters

Sugar palm (Arenga pinnata) is a feather palm native to tropical Asia. In Vietnam, it is named Búng Báng or Đoác and grown only on the highlands in the central or northern part of Vietnam. It is utilized for many purposes, especially for Ta Vat wine production - a characteristic and unique product of Co Tu ethnic minority. However, because of the natural fermentation used in the production, the product quality is inconsistent. The purpose of this study was to examine a new procedure of using palm sap for making Ta Vat wine. Some characteristics of the sap, which was collected at Nam Giang district, Quang Nam province are determined, proving the potential of the sap for making wine product. The quality of sap changes quickly at room temperature. At low temperature (4 - 60C), the changes in sap quality are apparently slower. Examining some factors affecting its quality during the wine fermentation process, we determined the best parameters for the fermentation process as follows: inoculum size of 3% with cell density of about 1x108 cells/ml, the addition of the extract from the bark of Ceylon ironwood (Mesua ferrea L.) 4%. Keywords: Arenga pinnata, sap, Ceylon ironwood bark, Mesua ferrea L., wine fermentation.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

Typical errors and violations of biotechnics of artificial breeding of pacific salmon

2020 ◽  
pp. 13-23
Author(s):  
Ekaterina Grinberg
Keyword(s):  
Production Process ◽  
Preventive Measures ◽  
Pacific Salmon ◽  
Fish Farming ◽  
Important Condition ◽  
The Third ◽  
Fish Breeding ◽  
Artificial Breeding ◽  
Strict Compliance

In two parts of the article, more than 70 errors and violations of the Biotechnics of artificial breeding of Pacific salmon in salmon hatcheries are collected and summarized, which lead to an immediate or delayed deterioration of the quality of reared fry and a decrease in their survival rate. Deviations from the biological basis of fish farming are shown and possible consequences of such errors or violations are predicted. In the first part of the article (#7,2020) there were General errors (throughout the entire fish-breeding process), as well as violations of Biotechnics during the work with producers, collecting eggs and preparing them for incubation, in the second part-violations of Biotechnics during the incubation of eggs, holding pre-larvae, rearing and release of young. Their correction, prevention or minimization of consequences will significantly improve the quality of sexual products and offspring from producers, increase the linear and weight gain of juveniles during rearing, improve the coefficient of feed payment, reduce the time of rearing, improve the epizootic situation by developing immunity in fry and mobilizing other protective reactions of their body, significantly reduce waste at all stages of the production process, increasing survival by 10–15%. In conclusion, it is noted that the first and most important condition for an efficient salmon hatchery is the creation of optimal environmental conditions at each stage of the production process. The second condition is strict compliance with the Biotechnics of artificial salmon breeding and the complex of veterinary and sanitary, fish-breeding and meliorative and therapeutic and preventive measures. The third is the availability of competent specialists who regularly improve their skills in accredited Universities to implement, control and manage the first two conditions.

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