scholarly journals Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

2018 ◽  
Vol 11 ◽  
pp. 38-42 ◽  
Author(s):  
Mukti Ram Paudel ◽  
Bijaya Pant
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Cell Growth Inhibition ◽  
Acid Methyl ◽  
High Cytotoxic Activity ◽  
Derivatives Of

 Dendrobium amoenum is an epiphytic orchid used as tonic because it has many derivatives of phenols. The crude extract of this orchid has been shown to have antioxidant activity. The objectives of this research are to explore the cytotoxic activity of antioxidant-rich crude extract against the human cervical carcinoma and glioblastoma cell lines by MTT assay and to detect the compounds by GC-MS. Methanol (DAM) extract of D. amoenum showed high cell growth inhibition percentage against the tested cell lines. DAM extract showed high cytotoxic activity against HeLa cells (IC50 – 110.22 μg/ml) and least activity against U-251 cells (IC50 – 550.55 μg/ml). Thirteen compounds were detected and identified in the extract. Based on abundance, four major compounds detected were: (E)-13-docosenoic acid; oleic acid; 11-octadecenoic acid, methyl ester; and hexadecanoic acid, 2,3-dihydroxypropyl ester. The cytotoxic activity of DAM extract is probably due to the presence of these bioactive compounds, confirmation of which needs further investigation. The result also highlighted the potential of this orchid as the source of natural anticancer drug and to explore their isolation, identification and characterization.Botanica Orientalis – Journal of Plant Science (2017) 11: 38–42

scholarly journals Bioassay-guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-resistant Staphylococcus aureus

2021 ◽  
Author(s):  
Suwaiba Muhammad Ladan ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Musa Yusuf ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Diethyl Ether ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Public Health Problem ◽  
Methicillin Resistant ◽  
Acid Methyl ◽  
Senna Alata

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

scholarly journals ANTIDIABETES ACTIVITIES EXTRACT HEXANE FROM THE PEELS OF ARTOCARPUS CAMANSI BLANCO FRUIT

2018 ◽  
Vol 11 (13) ◽  
pp. 12
Author(s):  
Rosnani Nasution ◽  
Chairani Nur Fitrah ◽  
Hira Helwati ◽  
Murniana Murniana ◽  
Bastian Arifin ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Methyl Ester ◽  
Crude Extract ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Activity Test ◽  
Acid Methyl ◽  
Test Result

 Objective: The purpose of this research is to know the chemical compound from the n-hexane extract of Kulu (Artocarpus camansi Blanco) fruit peels and to know its antidiabetes activity against male Swiss Webster mice.Methods: Samples were macerated with n-hexane solvent, then the extracts were obtained, was characterization with gas chromatography-mass spectrometry (GC-MS), and then the extract separated by column chromatography. The results obtained were tested by antidiabetes action with glucose tolerance method.Results: Anti-diabetic activity test result showed that the most active sample to decrease the blood glucose of mice was n-hexane crude extract, at minute 90th, activeness was 65.59%, compared to glibenclamide, then group of fraction A, at minute 90th, activeness 65.58% compared glibenclamide, whereas isolate D11, its activity is 60.11% and 60.12%, at minute 60th and 90 min compared to glibenclamide. Concentrated extracts were characterized by GC-MS, shows the major compounds are hexadecanoic acid methyl ester (30.14%), 9,12-octadecadienoyl chloride, (Z, Z) - (8.44%) and 9-octadecenoic acid, methyl ester (30,91%) also were obtained a compound like an β-amyrin acetate.Conclusion: The most active sample to decrease the blood glucose of mice was n-hexane crude extract, and the major compounds are hexadecanoic acid methyl ester (30.14%), 9,12-octadecadienoyl chloride, (Z, Z) - (8.44%) and 9-octadecenoic acid, methyl ester (30.91%).

scholarly journals Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 1 (3) ◽  
pp. 520-536
Author(s):  
Suwaiba Ladan Muhammad ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Yusuf Musa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Diethyl Ether ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Methicillin Resistant ◽  
Acid Methyl ◽  
Senna Alata

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study, suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

Identification of Potential Antimicrobial Compounds from A Marine Streptomyces Sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria

2021 ◽  
Vol 17 ◽  
Author(s):  
Nesrine Boublenza ◽  
Nadir Boudjlal Dergal ◽  
Larbi Belyagoubi ◽  
Noujoud Gabed ◽  
Djamel-Eddine Abdelouahid ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Acid Methyl Ester ◽  
Bioactive Metabolites ◽  
Streptomyces Sp ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Western Algeria ◽  
Marine Streptomyces

Background: Marine actinobacteria are a potential resource for natural products; their secondary bioactive metabolites have shown several biological activities. Most of the isolated and identified actinobacteria in Algeria were usually explored from caves, Saharan soil or palm groves. The marine ecosystem is poorly explored and documented. Methods: Five Streptomyces strains producing bioactive compounds were isolated from Rachgoun Island located in Western Algeria and characterised phenotypically and genotypically using microbiological and 16S rRNA sequencing methods, respectively. The crude extract of the most representative strain “Streptomyces sp. strain SM2.4” and its seven active fractions were characterised by GC/MS analysis. Results: Streptomyces sp. strain SM2.4 revealed the strongest activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis, the fungus Aspergillus niger and was inactive against Gram-negative bacteria. GC/MS analysis of the methylated crude extract of Streptomyces sp. strain SM2.4 revealed the presence of 11 major compounds including fatty acids methyl ester (12-methyltridecanoic acid methyl ester, 9-hexadecenoic acid methyl ester, hexadecanoic acid methyl ester, 14-methylhexadecanoic acid methyl ester and 16-methylheptadecanoic acid methyl ester), 2,4-di-tert-butylphenol, (4S,4aS,8aR)-4,8a-dimethyloctahydro-4a(2H)-naphthalenol (geosmin), 2,4-dimethylbenzaldehyde, 3,4-difluorobenzaldehyde, dimethylfuran-2,4-dicarboxylate and pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-. Partial purification of the crude extract by Thin-layer chromatography provided seven active fractions which were tested by radial diffusion assay. GC/MS analysis of the active TLC-fractions revealed the presence of a mixture of active compounds from which 2-(bromomethyl)-2-(2-methylphenyl)-1,3-dioxolane was found to be a new 1,3 dioxolane derivative. Furthermore, 3,4-dimethylbenzamide and pyrido[2,3-d] pyridazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, were extracted for the first time from a natural source. Conclusion: Our study reveals that marine Streptomyces sp. strain SM2.4 has an interesting antimicrobial potential due to its panel of bioactive compounds.

scholarly journals Sandalwood, an Indian medicinal plant attenuates the microbial growth and influence up/down regulation of the metabolites

2021 ◽  
Author(s):  
Romana Parveen ◽  
Tooba Naz Shamsi ◽  
Sadaf Fatima
Keyword(s):  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Trimethylsilyl Ester ◽  
E Coli ◽  
Acid Methyl ◽  
Soxhlet Apparatus ◽  
Antibacterial Assay

AbstractThe methanolic extract of sandalwood (SwME) was prepared by soxhlet apparatus and the antibacterial assay was performed. Further, the metabolite profiling of SwME and lysates of E. coli and E. coli grown in the presence of SwME was generated. SwME showed maximum inhibition against E. coli (MTCC 443) i.e. 82.71%, and minimal against B. subtilis (MTCC 736) i.e. 26.82%. The metabolome profiles of E. coli and SwME were generated using gas chromatography-mass spectrometry (GC-MS) technique. Comparative studies were done to understand to what extent metabolite modifications differ between SwME, E. coli lysate and the E. coli strain grown in presence of extract. Result revealed 23 peaks with major compounds present in E. coli were 9-Octadecenoic Acid (Z)-, Methyl Ester (26.85%), Hexadecanoic Acid, methyl ester (20.5%) and Hexadecanoic acid, trimethylsilyl ester (15.79%). When E. coli was grown in the presence of SwME, GC-MS analysis showed 25 peaks with major compounds such as 9-Octadecenoic Acid, Methyl Ester (21.97%), Hexadecanoic Acid, Methyl Ester (17.03%), and Hexadecanoic Acid, Trimethylsilyl Ester (14.96%). Correlating the metabolic profiles with the changes occurring is essential to progression their comprehension and in the development of new approaches to identify the metabolomics regulation in E. coli in response to SwME.

scholarly journals Arylsulfonamides From the Roots and Rhizomes of Tupistra chinensis Baker

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2092166
Author(s):  
Yue Xu ◽  
Xiaofei Liang ◽  
Yuze Li ◽  
Zhuofei Liang ◽  
Wenli Huang ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Cell Lines ◽  
Human Cancer ◽  
Acid Methyl Ester ◽  
Spectroscopic Methods ◽  
Tumor Cell Lines ◽  
Human Cancer Cell Lines ◽  
Acid Methyl ◽  
Amino Benzoic Acid

One new arylsulfonamide (1) and a novel natural product (2) were isolated from the roots and rhizomes of Tupistra chinensis Baker. Their structures were characterized by physicochemical properties and spectroscopic methods, as 2-[2-([1,1′-biphenyl]-4-ylsulfonylamino)-benzoylamino]-benzoic acid methyl ester (1), 2-[([1,1′-biphenyl]-4-ylsulfonyl)amino]-benzoic acid (2), respectively. Additionally, the cytotoxic activity of 1-2 was evaluated on human HCT116, HT29, A549, and H1299 tumor cell lines in vitro, respectively. Whereas, the result showed that these compounds displayed weak cytotoxicity in the human cancer cell lines.

scholarly journals Phytochemical Profiling and Larval Control of Erythrina variegata Methanol Fraction against Malarial and Filarial Vector

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mathalaimuthu Baranitharan ◽  
Barbara Sawicka ◽  
Jayapal Gokulakrishnan
Keyword(s):  
Methyl Ester ◽  
Methanol Extract ◽  
Mosquito Control ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Methanol Fraction ◽  
Mass Spectral ◽  
Erythrina Variegata ◽  
Acid Methyl

Erythrina variegata (E. variegata) bioactive chemical has been the potential to be utilized as a good, eco-friendly approach for the control of mosquito population. In the present investigation, methanol extract using insecticidal compounds isolated against mosquito larvae kill assay was carried out. Secondary metabolism was characterized by thin layer chromatography, column chromatography, Fourier transform-infrared spectroscopy, gas chromatography-mass spectral, and identification of compound. Mosquito immature third instar larval, Anopheles stephensi, and Culex quinquefasciatus have been exposed to different concentrations of 50-250 µg/ml. Totally, larvae were death rate 98.2% (significant value 0.001b) from methanol extract and it is significant toxicity against larvae of An. stephensi and Cx. quinquefasciatus with LC50/LC99 values were 157.69/339.55 µg/ml and 137.67/297.33 µg/ml, respectively. FT-IR analysis in the functional groups such as alcohol, amines, amides, alkenes, 1⁎ amines, aromatic amines, aliphatic amines, 1⁎,2⁎ amines, and alkyl halides searched the identity of secondary metabolites, which may act as 12-Octadecenoic acid, methyl ester compound and clearly indicates being phytochemical. Chemical constituents of twenty-five compounds were identified in the methanol extract. The major components were 12-Octadecenoic acid and methyl ester (37.31%). Compound molecules consist of carbon 19 atoms (gray), hydrogen 36 atoms (greenish blue), and oxygen 2 atoms (red), indicated by the different colors. The results were obtained suggesting that, in addition to their pharmaceutical and medicine sources, 12-Octadecenoic acid, methyl ester compound can also serve as a natural mosquito control.

scholarly journals Calendula officinalis: Potential Roles in Cancer Treatment and Palliative Care

2018 ◽  
Vol 17 (4) ◽  
pp. 1068-1078 ◽  
Author(s):  
Daniel Cruceriu ◽  
Ovidiu Balacescu ◽  
Elena Rakosy
Keyword(s):  
Palliative Care ◽  
Side Effects ◽  
Animal Models ◽  
Cancer Treatment ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Cancer Cell Lines ◽  
Calendula Officinalis

A continuous challenge in cancer management is to improve treatment efficacy and to diminish its side effects. Consequently, new conventional and unconventional drugs and bioactive compounds from plants are constantly developed, characterized, and used for in vitro and in vivo models. This review focuses on the antitumor properties of Calendula officinalis, its biological and molecular effects in tumor cells and animal models, as well as its role in cancer palliative care. A systematic review of studies describing the cytotoxic role of C officinalis and its therapeutic role on cancer cells were carried out using the PubMed database. Albeit C officinalis extracts have cytotoxic activity toward different cancer cell lines, a high grade of variation between studies was observed, depending on plant organ subjected to extraction, extraction method, and the cancer cell lines used for each study. Nevertheless, its cytotoxic activity is related to a few bioactive compounds, presenting multiple roles in both activation of proapoptotic proteins and decreasing the expression of the proteins that inhibit cell death. Moreover, due to its anti-genotoxic/protective as well as antitumor and antimetastatic effects proven in animal models, C officinalis could have important future implications in developing novel cancer treatment strategies, while until now it has been used especially for diminishing the side effects of radiotherapy.

scholarly journals Synthesis and Evaluation of Antimicrobial and Cytotoxic Activity of Oxathiine-Fused Quinone-Thioglucoside Conjugates of Substituted 1,4-Naphthoquinones

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3577
Author(s):  
Yuri E. Sabutski ◽  
Ekaterina S. Menchinskaya ◽  
Ludmila S. Shevchenko ◽  
Ekaterina A. Chingizova ◽  
Artur R. Chingizov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Hydroxyl Group ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
High Cytotoxic Activity ◽  
Derivatives Of

A series of new tetracyclic oxathiine-fused quinone-thioglycoside conjugates based on biologically active 1,4-naphthoquinones and 1-mercapto derivatives of per-O-acetyl d-glucose, d-galactose, d-xylose, and l-arabinose have been synthesized, characterized, and evaluated for their cytotoxic and antimicrobial activities. Six tetracyclic conjugates bearing a hydroxyl group in naphthoquinone core showed high cytotoxic activity with EC50 values in the range of 0.3 to 0.9 μM for various types of cancer and normal cells and no hemolytic activity up to 25 μM. The antimicrobial activity of conjugates was screened against Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), and fungus Candida albicans by the agar diffusion method. The most effective juglone conjugates with d-xylose or l-arabinose moiety and hydroxyl group at C-7 position of naphthoquinone core at concentration 10 µg/well showed antimicrobial activity comparable with antibiotics vancomicin and gentamicin against Gram-positive bacteria strains. In liquid media, juglone-arabinosidic tetracycles showed highest activity with MIC 6.25 µM. Thus, a positive effect of heterocyclization with mercaptosugars on cytotoxic and antimicrobial activity for group of 1,4-naphthoquinones was shown.

scholarly journals Antimicrobial Chlorinated 3-Phenylpropanoic Acid Derivatives from the Red Sea Marine Actinomycete Streptomyces coelicolor LY001

Marine Drugs ◽  
2020 ◽  
Vol 18 (9) ◽  
pp. 450 ◽  
Author(s):  
Lamiaa A. Shaala ◽  
Diaa T. A. Youssef ◽  
Torki A. Alzughaibi ◽  
Sameh S. Elhady
Keyword(s):  
Streptomyces Coelicolor ◽  
Acid Methyl Ester ◽  
Propanoic Acid ◽  
Ionization Mass ◽  
Acid Methyl ◽  
Marine Actinomycete ◽  
Actinomycete Strain ◽  
Derivatives Of ◽  
And Staphylococcus Aureus ◽  
Insight Into

The actinomycete strain Streptomyces coelicolor LY001 was purified from the sponge Callyspongia siphonella. Fractionation of the antimicrobial extract of the culture of the actinomycete afforded three new natural chlorinated derivatives of 3-phenylpropanoic acid, 3-(3,5-dichloro-4-hydroxyphenyl)propanoic acid (1), 3-(3,5-dichloro-4-hydroxyphenyl)propanoic acid methyl ester (2), and 3-(3-chloro-4-hydroxyphenyl)propanoic acid (3), together with 3-phenylpropanoic acid (4), E-cinnamic acid (5), and the diketopiperazine alkaloids cyclo(l-Phe-trans-4-OH-l-Pro) (6) and cyclo(l-Phe-cis-4-OH-d-Pro) (7) were isolated. Interpretation of nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HRESIMS) data of 1–7 supported their assignments. Compounds 1–3 are first candidates of the natural chlorinated phenylpropanoic acid derivatives. The production of the chlorinated derivatives of 3-phenylpropionic acid (1–3) by S. coelicolor provides insight into the biosynthetic capabilities of the marine-derived actinomycetes. Compounds 1–3 demonstrated significant and selective activities towards Escherichia. coli and Staphylococcus aureus, while Candida albicans displayed more sensitivity towards compounds 6 and 7, suggesting a selectivity effect of these compounds against C. albicans.

Sign in / Sign up

Export Citation Format

Share Document